Restriction map for
pFN21AB9498
(Linear) (Six-base) MAPSORT of: KIBB9498.seq Check: 7244 from: 1 to: 1833
KIBB9498.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AgeI A'CCGG_T
Cuts at: 0 692 1833
Size: 692 1141
ApoI r'AATT_y
Cuts at: 0 340 427 494 1833
Size: 340 87 67 1339
Fragments arranged by size:
1339 340 87 67
AvaI C'yCGr_G
Cuts at: 0 239 950 1064 1199 1833
Size: 239 711 114 135 634
Fragments arranged by size:
711 634 239 135 114
BanI G'GyrC_C
Cuts at: 0 192 1833
Size: 192 1641
BanII G_rGCy'C
Cuts at: 0 603 934 1492 1833
Size: 603 331 558 341
Fragments arranged by size:
603 558 341 331
BbeI G_GCGC'C
Cuts at: 0 196 1833
Size: 196 1637
BbsI GAAGACnn'nnnn_
Cuts at: 0 43 259 902 1833
Size: 43 216 643 931
Fragments arranged by size:
931 643 216 43
BbvCI CC'TCA_GC
Cuts at: 0 9 65 1833
Size: 9 56 1768
Fragments arranged by size:
1768 56 9
BclI T'GATC_A
Cuts at: 0 513 1401 1833
Size: 513 888 432
Fragments arranged by size:
888 513 432
BglII A'GATC_T
Cuts at: 0 1711 1833
Size: 1711 122
Bme1580I G_kGCm'C
Cuts at: 0 459 1833
Size: 459 1374
BmrI ACTGGGnnnn_n'
Cuts at: 0 1050 1289 1628 1833
Size: 1050 239 339 205
Fragments arranged by size:
1050 339 239 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 918 1523 1692 1833
Size: 615 303 605 169 141
Fragments arranged by size:
615 605 303 169 141
Bpu10I CC'TnA_GC
Cuts at: 0 9 65 1721 1833
Size: 9 56 1656 112
Fragments arranged by size:
1656 112 56 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1652 1833
Size: 1652 181
BsaAI yAC'GTr
Cuts at: 0 382 1833
Size: 382 1451
BsaBI GATnn'nnATC
Cuts at: 0 563 707 719 746 1833
Size: 563 144 12 27 1087
Fragments arranged by size:
1087 563 144 27 12
BsaHI Gr'CG_yC
Cuts at: 0 193 1833
Size: 193 1640
BsaWI w'CCGG_w
Cuts at: 0 257 692 1833
Size: 257 435 1141
Fragments arranged by size:
1141 435 257
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 588 618 1308 1338 1833
Size: 588 30 690 30 495
Fragments arranged by size:
690 588 495 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 890 1833
Size: 890 943
BseYI C'CCAG_C
Cuts at: 0 116 388 1833
Size: 116 272 1445
Fragments arranged by size:
1445 272 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1700 1833
Size: 386 1314 133
Fragments arranged by size:
1314 386 133
BsiEI CG_ry'CG
Cuts at: 0 258 925 1252 1833
Size: 258 667 327 581
Fragments arranged by size:
667 581 327 258
BsiHKAI G_wGCw'C
Cuts at: 0 934 1492 1833
Size: 934 558 341
Fragments arranged by size:
934 558 341
BsmI GAATG_Cn'
Cuts at: 0 1449 1605 1833
Size: 1449 156 228
Fragments arranged by size:
1449 228 156
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1637 1833
Size: 1637 196
Bsp1286I G_dGCh'C
Cuts at: 0 459 603 934 1492 1833
Size: 459 144 331 558 341
Fragments arranged by size:
558 459 341 331 144
BsrFI r'CCGG_y
Cuts at: 0 692 1833
Size: 692 1141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 116 1833
Size: 116 1717
BstYI r'GATC_y
Cuts at: 0 1711 1833
Size: 1711 122
Bsu36I CC'TnA_GG
Cuts at: 0 1025 1736 1833
Size: 1025 711 97
Fragments arranged by size:
1025 711 97
BtgI C'CryG_G
Cuts at: 0 229 1632 1833
Size: 229 1403 201
Fragments arranged by size:
1403 229 201
BtsI GCAGTG_nn'
Cuts at: 0 221 287 374 831 852 1833
Size: 221 66 87 457 21 981
Fragments arranged by size:
981 457 221 87 66 21
ClaI AT'CG_AT
Cuts at: 0 750 1833
Size: 750 1083
EaeI y'GGCC_r
Cuts at: 0 552 759 1833
Size: 552 207 1074
Fragments arranged by size:
1074 552 207
EarI CTCTTCn'nnn_
Cuts at: 0 320 467 636 828 1833
Size: 320 147 169 192 1005
Fragments arranged by size:
1005 320 192 169 147
EcoICRI GAG'CTC
Cuts at: 0 932 1490 1833
Size: 932 558 343
Fragments arranged by size:
932 558 343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 918 1523 1692 1833
Size: 615 303 605 169 141
Fragments arranged by size:
615 605 303 169 141
EcoNI CCTnn'n_nnAGG
Cuts at: 0 641 1833
Size: 641 1192
EcoO109I rG'GnC_Cy
Cuts at: 0 435 979 1283 1833
Size: 435 544 304 550
Fragments arranged by size:
550 544 435 304
EcoRI G'AATT_C
Cuts at: 0 340 494 1833
Size: 340 154 1339
Fragments arranged by size:
1339 340 154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 360 1833
Size: 360 1473
HaeII r_GCGC'y
Cuts at: 0 196 1833
Size: 196 1637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 344 376 588 620 907 931 939 963
Size: 344 32 212 32 287 24 8 24
Cuts at: 963 1024 1056 1357 1389 1492 1524 1690 1696
Size: 61 32 301 32 103 32 166 6
Cuts at: 1696 1722 1728 1833
Size: 26 6 105
Fragments arranged by size:
344 301 287 212 166 105 103 61
32 32 32 32 32 26 24 24
8 6 6
HincII GTy'rAC
Cuts at: 0 1101 1833
Size: 1101 732
KasI G'GCGC_C
Cuts at: 0 192 1833
Size: 192 1641
MfeI C'AATT_G
Cuts at: 0 200 1833
Size: 200 1633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 1833
Size: 1402 431
MspA1I CmG'CkG
Cuts at: 0 116 1108 1170 1833
Size: 116 992 62 663
Fragments arranged by size:
992 663 116 62
NarI GG'CG_CC
Cuts at: 0 193 1833
Size: 193 1640
PfoI T'CCnGG_A
Cuts at: 0 814 1638 1833
Size: 814 824 195
Fragments arranged by size:
824 814 195
PmlI CAC'GTG
Cuts at: 0 382 1833
Size: 382 1451
PpuMI rG'GwC_Cy
Cuts at: 0 435 979 1283 1833
Size: 435 544 304 550
Fragments arranged by size:
550 544 435 304
PspXI vC'TCGA_Gb
Cuts at: 0 1064 1833
Size: 1064 769
PvuII CAG'CTG
Cuts at: 0 116 1108 1170 1833
Size: 116 992 62 663
Fragments arranged by size:
992 663 116 62
SacI G_AGCT'C
Cuts at: 0 934 1492 1833
Size: 934 558 341
Fragments arranged by size:
934 558 341
SapI GCTCTTCn'nnn_
Cuts at: 0 828 1833
Size: 828 1005
ScaI AGT'ACT
Cuts at: 0 1020 1833
Size: 1020 813
SfcI C'TryA_G
Cuts at: 0 611 870 1138 1833
Size: 611 259 268 695
Fragments arranged by size:
695 611 268 259
SfoI GGC'GCC
Cuts at: 0 194 1833
Size: 194 1639
SmlI C'TyrA_G
Cuts at: 0 1064 1833
Size: 1064 769
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 794 911 914 1226 1440 1833
Size: 794 117 3 312 214 393
Fragments arranged by size:
794 393 312 214 117 3
TatI w'GTAC_w
Cuts at: 0 1018 1833
Size: 1018 815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 810 842 1248 1280 1308 1340 1833
Size: 810 32 406 32 28 32 493
Fragments arranged by size:
810 493 406 32 32 32 28
XbaI T'CTAG_A
Cuts at: 0 1335 1833
Size: 1335 498
XhoI C'TCGA_G
Cuts at: 0 1064 1833
Size: 1064 769
XmnI GAAnn'nnTTC
Cuts at: 0 344 1833
Size: 344 1489
Enzymes that do cut:
AgeI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BclI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrFI BstXI BstYI Bsu36I
BtgI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MfeI
MmeI MspA1I NarI PfoI PmlI PpuMI PspXI PvuII
SacI SapI ScaI SfcI SfoI SmlI TaqII TatI
TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsiWI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraI DraIII
DrdI EagI EciI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MluI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XcmI XmaI ZraI