(Circular) (Six-base) MAPSORT of: pFN21AB9479.seq Check: 3978 from: 1 to: 6898 pFN21AB9479 6898 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3931 3931 Size: 6898 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4708 278 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4241 1937 Size: 2304 4594 Acc65I G'GTAC_C Cuts at: 4219 4219 Size: 6898 AclI AA'CG_TT Cuts at: 1563 5025 5398 1563 Size: 3462 373 3063 Fragments arranged by size: 3462 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 3167 4054 4118 4961 6345 6613 1632 Size: 1244 291 887 64 843 1384 268 1917 Fragments arranged by size: 1917 1384 1244 887 843 291 268 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6701 AflIII A'CryG_T Cuts at: 1203 2592 5797 6774 1203 Size: 1389 3205 977 1327 Fragments arranged by size: 3205 1389 1327 977 AgeI A'CCGG_T Cuts at: 5729 5729 Size: 6898 AhdI GACnn_n'nnGTC Cuts at: 5627 5627 Size: 6898 AleI CACnn'nnGTG Cuts at: 1166 2333 2462 1166 Size: 1167 129 5602 Fragments arranged by size: 5602 1167 129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6898 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3126 3158 3126 Size: 32 6866 AlwNI CAG_nnn'CTG Cuts at: 2024 2105 2365 2392 2663 2899 3004 3025 3238 Size: 81 260 27 271 236 105 21 213 Cuts at: 3238 3322 3331 3847 4641 6213 2024 Size: 84 9 516 794 1572 2709 Fragments arranged by size: 2709 1572 794 516 271 260 236 213 105 84 81 27 21 9 ApaI G_GGCC'C Cuts at: 2081 2611 2676 3013 3050 3617 4155 2081 Size: 530 65 337 37 567 538 4824 Fragments arranged by size: 4824 567 538 530 337 65 37 ApaLI G'TGCA_C Cuts at: 4955 6111 4955 Size: 1156 5742 ApoI r'AATT_y Cuts at: 1493 3406 4207 4433 4585 6694 1493 Size: 1913 801 226 152 2109 1697 Fragments arranged by size: 2109 1913 1697 801 226 152 AseI AT'TA_AT Cuts at: 160 1032 5452 160 Size: 872 4420 1606 Fragments arranged by size: 4420 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 AvaI C'yCGr_G Cuts at: 1870 1943 2711 4223 1870 Size: 73 768 1512 4545 Fragments arranged by size: 4545 1512 768 73 BamHI G'GATC_C Cuts at: 4228 4595 4228 Size: 367 6531 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2249 2524 3384 3754 4219 Size: 325 217 627 462 275 860 370 465 Cuts at: 4219 5674 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 860 627 465 462 370 325 275 217 BanII G_rGCy'C Cuts at: 1391 2081 2116 2381 2611 2676 2953 2983 3013 Size: 690 35 265 230 65 277 30 30 Cuts at: 3013 3050 3617 4139 4155 4217 1391 Size: 37 567 522 16 62 4072 Fragments arranged by size: 4072 690 567 522 277 265 230 65 62 37 35 30 30 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2804 928 Size: 971 905 5022 Fragments arranged by size: 5022 971 905 BbvCI CC'TCA_GC Cuts at: 2514 3171 2514 Size: 657 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5089 5123 1903 Size: 34 3152 34 3678 Fragments arranged by size: 3678 3152 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4790 6000 4790 Size: 1210 5688 BclI T'GATC_A Cuts at: 1548 1548 Size: 6898 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 2917 3377 Size: 107 122 71 631 605 448 797 460 Cuts at: 3377 5509 136 Size: 2132 1525 Fragments arranged by size: 2132 1525 797 631 605 460 448 122 107 71 BglII A'GATC_T Cuts at: 6893 6893 Size: 6898 BlpI GC'TnA_GC Cuts at: 3294 3465 4315 3294 Size: 171 850 5877 Fragments arranged by size: 5877 850 171 Bme1580I G_kGCm'C Cuts at: 1790 2081 2252 2529 2611 2676 3013 3050 3617 Size: 291 171 277 82 65 337 37 567 Cuts at: 3617 3757 3790 4155 4167 4959 6115 1790 Size: 140 33 365 12 792 1156 2573 Fragments arranged by size: 2573 1156 792 567 365 337 291 277 171 140 82 65 37 33 12 BmgBI CAC'GTC Cuts at: 1318 2464 1318 Size: 1146 5752 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2084 2679 2765 Size: 416 53 83 399 24 667 595 86 Cuts at: 2765 3349 3526 3824 4629 5587 442 Size: 584 177 298 805 958 1753 Fragments arranged by size: 1753 958 805 667 595 584 416 399 298 177 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3827 1056 Size: 2771 4127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3868 3868 Size: 6898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2425 3002 3005 3047 3185 3260 3353 3678 Size: 1186 577 3 42 138 75 93 325 Cuts at: 3678 4013 5558 1239 Size: 335 1545 2579 Fragments arranged by size: 2579 1545 1186 577 335 325 138 93 75 42 3 Bpu10I CC'TnA_GC Cuts at: 2514 2750 3171 3769 2514 Size: 236 421 598 5643 Fragments arranged by size: 5643 598 421 236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5023 5888 6186 5023 Size: 865 298 5735 Fragments arranged by size: 5735 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2307 2318 3364 3447 5561 882 Size: 617 808 11 1046 83 2114 2219 Fragments arranged by size: 2219 2114 1046 808 617 83 11 BsaAI yAC'GTr Cuts at: 493 1204 4689 493 Size: 711 3485 2702 Fragments arranged by size: 3485 2702 711 BsaBI GATnn'nnATC Cuts at: 6892 6892 Size: 6898 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4705 5087 5722 275 Size: 53 83 186 936 3172 382 635 1451 Fragments arranged by size: 3172 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3456 5331 5729 6003 6150 6561 3456 Size: 1875 398 274 147 411 3793 Fragments arranged by size: 3793 1875 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2286 2316 1178 Size: 30 165 30 717 30 136 30 5760 Fragments arranged by size: 5760 717 165 136 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2246 2441 2627 2939 2942 3259 3593 3643 Size: 1060 195 186 312 3 317 334 50 Cuts at: 3643 4022 1186 Size: 379 4062 Fragments arranged by size: 4062 1060 379 334 317 312 195 186 50 3 BseYI C'CCAG_C Cuts at: 2034 2162 2549 2575 2792 2865 2937 3217 3274 Size: 128 387 26 217 73 72 280 57 Cuts at: 3274 3298 3890 6101 6597 2034 Size: 24 592 2211 496 2335 Fragments arranged by size: 2335 2211 592 496 387 280 217 128 73 72 57 26 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2583 1163 Size: 573 847 5478 Fragments arranged by size: 5478 847 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4348 5109 6137 1540 Size: 275 186 2347 761 1028 2301 Fragments arranged by size: 2347 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3471 4959 5044 6115 3471 Size: 1488 85 1071 4254 Fragments arranged by size: 4254 1488 1071 85 BsmI GAATG_Cn' Cuts at: 2220 2938 4409 4502 2220 Size: 718 1471 93 4616 Fragments arranged by size: 4616 1471 718 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2479 2479 Size: 6898 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2116 2252 2381 2529 2611 2676 Size: 399 291 35 136 129 148 82 65 Cuts at: 2676 2953 2983 3013 3050 3471 3617 3757 3790 Size: 277 30 30 37 421 146 140 33 Cuts at: 3790 4139 4155 4167 4217 4959 5044 6115 1391 Size: 349 16 12 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 349 291 277 148 146 140 136 129 85 82 65 50 37 35 33 30 30 16 12 BspEI T'CCGG_A Cuts at: 6561 6561 Size: 6898 BspHI T'CATG_A Cuts at: 3955 4787 6517 3955 Size: 832 1730 4336 Fragments arranged by size: 4336 1730 832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2325 2686 3931 4253 844 Size: 1481 361 1245 322 3489 Fragments arranged by size: 3489 1481 1245 361 322 BsrBI CCG'CTC Cuts at: 1394 1823 2692 4785 1394 Size: 429 869 2093 3507 Fragments arranged by size: 3507 2093 869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5393 5567 65 Size: 1183 4145 174 1396 Fragments arranged by size: 4145 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2504 5542 5729 1700 Size: 110 694 3038 187 2869 Fragments arranged by size: 3038 2869 694 187 110 BsrGI T'GTAC_A Cuts at: 96 3226 96 Size: 3130 3768 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6898 BssSI C'ACGA_G Cuts at: 4958 5970 4958 Size: 1012 5886 BstAPI GCAn_nnn'nTGC Cuts at: 794 3004 794 Size: 2210 4688 BstBI TT'CG_AA Cuts at: 5702 5702 Size: 6898 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6898 BstXI CCAn_nnnn'nTGG Cuts at: 3353 3656 4082 3353 Size: 303 426 6169 Fragments arranged by size: 6169 426 303 BstYI r'GATC_y Cuts at: 1972 3235 4228 4595 4980 4997 6449 6535 6547 Size: 1263 993 367 385 17 1452 86 12 Cuts at: 6547 6893 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1263 993 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2331 2520 2676 3265 513 Size: 552 939 165 162 189 156 589 4146 Fragments arranged by size: 4146 939 589 552 189 165 162 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6159 Fragments arranged by size: 6159 683 56 BtsI GCAGTG_nn' Cuts at: 2452 4414 5208 5228 2452 Size: 1962 794 20 4122 Fragments arranged by size: 4122 1962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6898 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3662 3697 529 Size: 35 3098 35 3730 Fragments arranged by size: 3730 3098 35 35 DraI TTT'AAA Cuts at: 4202 4202 Size: 6898 DraIII CAC_nnn'GTG Cuts at: 3089 3089 Size: 6898 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2303 3356 5905 809 Size: 507 987 1053 2549 1802 Fragments arranged by size: 2549 1802 1053 987 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2829 3496 4345 Size: 54 1425 50 275 18 999 667 849 Cuts at: 4345 5234 6863 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 999 889 849 667 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4345 1537 Size: 275 2533 4090 Fragments arranged by size: 4090 2533 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 2645 2880 4097 4828 1353 Size: 842 450 235 1217 731 3423 Fragments arranged by size: 3423 1217 842 731 450 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5485 5859 6005 387 Size: 1064 138 3896 374 146 1280 Fragments arranged by size: 3896 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2876 3002 3005 3047 3167 3185 Size: 393 793 451 126 3 42 120 18 Cuts at: 3185 3260 3353 3678 4013 4054 4118 4961 5558 Size: 75 93 325 335 41 64 843 597 Cuts at: 5558 6345 6613 1239 Size: 787 268 1524 Fragments arranged by size: 1524 843 793 787 597 451 393 335 325 268 126 120 93 75 64 42 41 18 3 EcoNI CCTnn'n_nnAGG Cuts at: 2289 2289 Size: 6898 EcoO109I rG'GnC_Cy Cuts at: 2122 2362 2607 2608 2632 2698 2767 3046 3113 Size: 240 245 1 24 66 69 279 67 Cuts at: 3113 3271 3328 3349 3613 3799 4151 2122 Size: 158 57 21 264 186 352 4869 Fragments arranged by size: 4869 352 279 264 245 240 186 158 69 67 66 57 24 21 1 EcoRI G'AATT_C Cuts at: 3406 4207 4585 3406 Size: 801 378 5719 Fragments arranged by size: 5719 801 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3415 3415 Size: 6898 FseI GG_CCGG'CC Cuts at: 2508 2508 Size: 6898 FspI TGC'GCA Cuts at: 5404 5404 Size: 6898 HaeII r_GCGC'y Cuts at: 1678 6045 1678 Size: 4367 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 4112 4144 5539 Size: 32 135 32 515 32 1993 32 1395 Cuts at: 5539 5571 5613 5645 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1993 1395 515 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4242 4488 669 Size: 988 281 2304 246 3079 Fragments arranged by size: 3079 2304 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6898 HpaI GTT'AAC Cuts at: 4488 4488 Size: 6898 KpnI G_GTAC'C Cuts at: 4223 4223 Size: 6898 MfeI C'AATT_G Cuts at: 4497 4497 Size: 6898 MluI A'CGCG_T Cuts at: 6774 6774 Size: 6898 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6012 6196 1305 Size: 4707 184 2007 Fragments arranged by size: 4707 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2831 10 Size: 54 1425 343 999 4077 Fragments arranged by size: 4077 1425 999 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 2462 4856 5215 5374 518 Size: 648 1167 129 2394 359 159 2042 Fragments arranged by size: 2394 2042 1167 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2436 2761 2869 2977 3004 3163 3267 3898 Size: 830 325 108 108 27 159 104 631 Cuts at: 3898 4314 4991 6139 6384 1606 Size: 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 830 677 631 416 325 245 159 108 108 104 27 NaeI GCC'GGC Cuts at: 2506 2506 Size: 6898 NcoI C'CATG_G Cuts at: 513 1065 2004 2169 2520 2676 513 Size: 552 939 165 351 156 4735 Fragments arranged by size: 4735 939 552 351 165 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 6898 NgoMIV G'CCGG_C Cuts at: 2504 2504 Size: 6898 NheI G'CTAG_C Cuts at: 1052 3823 1052 Size: 2771 4127 NotI GC'GGCC_GC Cuts at: 4345 4345 Size: 6898 NspI r_CATG'y Cuts at: 1132 1229 2596 4256 5801 1132 Size: 97 1367 1660 1545 2229 Fragments arranged by size: 2229 1660 1545 1367 97 PasI CC'CwG_GG Cuts at: 1348 3117 3353 3760 3885 4155 1348 Size: 1769 236 407 125 270 4091 Fragments arranged by size: 4091 1769 407 270 236 125 PciI A'CATG_T Cuts at: 2592 5797 2592 Size: 3205 3693 PflMI CCAn_nnn'nTGG Cuts at: 1108 3025 3310 1108 Size: 1917 285 4696 Fragments arranged by size: 4696 1917 285 PfoI T'CCnGG_A Cuts at: 2087 3972 2087 Size: 1885 5013 PmeI GTTT'AAAC Cuts at: 4202 4202 Size: 6898 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4967 4999 6480 6512 4967 Size: 32 1481 32 5353 Fragments arranged by size: 5353 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2632 2698 3271 3349 2362 Size: 270 66 573 78 5911 Fragments arranged by size: 5911 573 270 78 66 PshAI GACnn'nnGTC Cuts at: 1613 2240 1613 Size: 627 6271 PsiI TTA'TAA Cuts at: 4468 4468 Size: 6898 PspOMI G'GGCC_C Cuts at: 2077 2607 2672 3009 3046 3613 4151 2077 Size: 530 65 337 37 567 538 4824 Fragments arranged by size: 4824 567 538 530 337 65 37 PstI C_TGCA'G Cuts at: 830 2121 2322 2766 2862 2874 2958 3000 3327 Size: 1291 201 444 96 12 84 42 327 Cuts at: 3327 4250 830 Size: 923 3478 Fragments arranged by size: 3478 1291 923 444 327 201 96 84 42 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6898 PvuII CAG'CTG Cuts at: 2761 2869 3004 3163 3898 2761 Size: 108 135 159 735 5761 Fragments arranged by size: 5761 735 159 135 108 SacII CC_GC'GG Cuts at: 3268 3268 Size: 6898 SalI G'TCGA_C Cuts at: 1936 4240 1936 Size: 2304 4594 SanDI GG'GwC_CC Cuts at: 3271 3271 Size: 6898 SapI GCTCTTCn'nnn_ Cuts at: 2195 2880 2195 Size: 685 6213 SbfI CC_TGCA'GG Cuts at: 2121 2322 4250 2121 Size: 201 1928 4769 Fragments arranged by size: 4769 1928 201 ScaI AGT'ACT Cuts at: 5146 6858 5146 Size: 1712 5186 SfcI C'TryA_G Cuts at: 826 1045 2117 2318 2762 2858 2870 2954 2996 Size: 219 1072 201 444 96 12 84 42 Cuts at: 2996 3323 4246 5381 6062 6253 826 Size: 327 923 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 923 681 444 327 219 201 191 96 84 42 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6898 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6898 SmaI CCC'GGG Cuts at: 1872 4225 1872 Size: 2353 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5002 5903 6165 820 Size: 197 926 3059 901 262 1553 Fragments arranged by size: 3059 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4689 493 Size: 4196 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6898 SphI G_CATG'C Cuts at: 1132 4256 1132 Size: 3124 3774 SspI AAT'ATT Cuts at: 5 52 4822 5 Size: 47 4770 2081 Fragments arranged by size: 4770 2081 47 StuI AGG'CCT Cuts at: 1517 2343 3380 1517 Size: 826 1037 5035 Fragments arranged by size: 5035 1037 826 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2520 2602 2676 2832 3569 Size: 552 939 165 351 82 74 156 737 Cuts at: 3569 3629 3903 4337 513 Size: 60 274 434 3074 Fragments arranged by size: 3074 939 737 552 434 351 274 165 156 82 74 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 4925 4942 5095 5280 5725 325 Size: 1255 446 2899 17 153 185 445 1498 Fragments arranged by size: 2899 1498 1255 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3226 5144 6856 Size: 274 80 33 51 1443 1249 1918 1712 Cuts at: 6856 96 Size: 138 Fragments arranged by size: 1918 1712 1443 1249 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2120 2152 3377 3409 3422 3454 6858 6890 2120 Size: 32 1225 32 13 32 3404 32 2128 Fragments arranged by size: 3404 2128 1225 32 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 2786 3038 1316 Size: 1470 252 5176 Fragments arranged by size: 5176 1470 252 XbaI T'CTAG_A Cuts at: 4234 4234 Size: 6898 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2683 3549 2527 Size: 156 866 5876 Fragments arranged by size: 5876 866 156 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6898 XmaI C'CCGG_G Cuts at: 1870 4223 1870 Size: 2353 4545 XmnI GAAnn'nnTTC Cuts at: 2889 5027 2889 Size: 2138 4760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4706 276 Size: 53 83 186 936 3172 2468 Fragments arranged by size: 3172 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI