(Circular) (Six-base) MAPSORT of: pFN21AB9467.seq Check: 2052 from: 1 to: 8248 pFN21AB9467 8248 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2503 2503 Size: 8248 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6058 278 Size: 53 83 186 936 4522 2468 Fragments arranged by size: 4522 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3585 3762 4991 5591 1937 Size: 1648 177 1229 600 4594 Fragments arranged by size: 4594 1648 1229 600 177 Acc65I G'GTAC_C Cuts at: 5569 5569 Size: 8248 AclI AA'CG_TT Cuts at: 1563 5267 6375 6748 1563 Size: 3704 1108 373 3063 Fragments arranged by size: 3704 3063 1108 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4030 4330 4694 4949 6311 7695 7963 1632 Size: 2398 300 364 255 1362 1384 268 1917 Fragments arranged by size: 2398 1917 1384 1362 364 300 268 255 AfeI AGC'GCT Cuts at: 2058 2058 Size: 8248 AflII C'TTAA_G Cuts at: 820 1017 4741 820 Size: 197 3724 4327 Fragments arranged by size: 4327 3724 197 AflIII A'CryG_T Cuts at: 1203 2276 2393 7147 8124 1203 Size: 1073 117 4754 977 1327 Fragments arranged by size: 4754 1327 1073 977 117 AgeI A'CCGG_T Cuts at: 7079 7079 Size: 8248 AhdI GACnn_n'nnGTC Cuts at: 6977 6977 Size: 8248 AleI CACnn'nnGTG Cuts at: 1166 3453 3908 1166 Size: 2287 455 5506 Fragments arranged by size: 5506 2287 455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4228 1184 Size: 3044 5204 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3921 3953 3921 Size: 32 8216 AlwNI CAG_nnn'CTG Cuts at: 2807 5991 7563 2807 Size: 3184 1572 3492 Fragments arranged by size: 3492 3184 1572 ApaLI G'TGCA_C Cuts at: 3540 4886 6305 7461 3540 Size: 1346 1419 1156 4327 Fragments arranged by size: 4327 1419 1346 1156 ApoI r'AATT_y Cuts at: 1493 2290 2581 2594 2974 3084 3282 3748 4791 Size: 797 291 13 380 110 198 466 1043 Cuts at: 4791 4815 4843 4913 5125 5238 5557 5783 5935 Size: 24 28 70 212 113 319 226 152 Cuts at: 5935 8044 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1043 797 466 380 319 291 226 212 198 152 113 110 70 28 24 13 AseI AT'TA_AT Cuts at: 160 1032 6802 160 Size: 872 5770 1606 Fragments arranged by size: 5770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8248 AvaI C'yCGr_G Cuts at: 1870 1943 3375 3641 4217 5573 1870 Size: 73 1432 266 576 1356 4545 Fragments arranged by size: 4545 1432 1356 576 266 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5000 5033 5000 Size: 33 8215 BamHI G'GATC_C Cuts at: 3821 5578 5945 3821 Size: 1757 367 6124 Fragments arranged by size: 6124 1757 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3920 4173 5569 7024 618 Size: 325 217 627 2133 253 1396 1455 1842 Fragments arranged by size: 2133 1842 1455 1396 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 4245 5541 5567 1391 Size: 2854 1296 26 4072 Fragments arranged by size: 4072 2854 1296 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3242 4336 928 Size: 971 1343 1094 4840 Fragments arranged by size: 4840 1343 1094 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6439 6473 1903 Size: 34 4502 34 3678 Fragments arranged by size: 4502 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6140 7350 6140 Size: 1210 7038 BclI T'GATC_A Cuts at: 1548 2893 1548 Size: 1345 6903 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3645 6859 136 Size: 107 122 71 631 605 1973 3214 1525 Fragments arranged by size: 3214 1973 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4829 8243 4829 Size: 3414 4834 BlpI GC'TnA_GC Cuts at: 5665 5665 Size: 8248 Bme1580I G_kGCm'C Cuts at: 1790 2413 3544 3788 4890 6309 7465 1790 Size: 623 1131 244 1102 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 1131 1102 623 244 BmgBI CAC'GTC Cuts at: 1318 3526 1318 Size: 2208 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5034 5979 6937 Size: 416 53 83 399 24 3617 945 958 Cuts at: 6937 442 Size: 1753 Fragments arranged by size: 3617 1753 958 945 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2405 2405 Size: 8248 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2230 2400 3235 3363 3774 3833 4126 6908 Size: 991 170 835 128 411 59 293 2782 Cuts at: 6908 1239 Size: 2579 Fragments arranged by size: 2782 2579 991 835 411 293 170 128 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4416 6373 7238 7536 4416 Size: 1957 865 298 5128 Fragments arranged by size: 5128 1957 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 5522 6911 882 Size: 617 609 3414 1389 2219 Fragments arranged by size: 3414 2219 1389 617 609 BsaAI yAC'GTr Cuts at: 493 1204 6039 493 Size: 711 4835 2702 Fragments arranged by size: 4835 2702 711 BsaBI GATnn'nnATC Cuts at: 4126 5073 8242 4126 Size: 947 3169 4132 Fragments arranged by size: 4132 3169 947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4721 6055 6437 7072 Size: 53 83 186 936 3188 1334 382 635 Cuts at: 7072 275 Size: 1451 Fragments arranged by size: 3188 1451 1334 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2126 6681 7079 7353 7500 7911 2126 Size: 4555 398 274 147 411 2463 Fragments arranged by size: 4555 2463 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8023 Fragments arranged by size: 8023 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3129 3363 1186 Size: 1943 234 6071 Fragments arranged by size: 6071 1943 234 BseYI C'CCAG_C Cuts at: 3065 3190 7451 7947 3065 Size: 125 4261 496 3366 Fragments arranged by size: 4261 3366 496 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 3177 3216 1163 Size: 573 444 997 39 6195 Fragments arranged by size: 6195 997 573 444 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5698 6459 7487 1540 Size: 275 186 3697 761 1028 2301 Fragments arranged by size: 3697 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3373 3531 3544 4224 4890 5541 6309 6394 7465 Size: 158 13 680 666 651 768 85 1071 Cuts at: 7465 3373 Size: 4156 Fragments arranged by size: 4156 1071 768 680 666 651 158 85 13 BsmI GAATG_Cn' Cuts at: 2310 4024 5759 5852 2310 Size: 1714 1735 93 4706 Fragments arranged by size: 4706 1735 1714 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4728 4728 Size: 8248 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2413 3373 3531 3544 3788 4224 4245 Size: 399 623 960 158 13 244 436 21 Cuts at: 4245 4890 5541 5567 6309 6394 7465 1391 Size: 645 651 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 960 742 651 645 623 436 399 244 158 85 26 21 13 BspEI T'CCGG_A Cuts at: 7911 7911 Size: 8248 BspHI T'CATG_A Cuts at: 6137 7867 6137 Size: 1730 6518 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2503 5189 5603 844 Size: 1659 2686 414 3489 Fragments arranged by size: 3489 2686 1659 414 BsrBI CCG'CTC Cuts at: 1394 1823 6135 1394 Size: 429 4312 3507 Fragments arranged by size: 4312 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2423 6743 6917 65 Size: 1183 1175 4320 174 1396 Fragments arranged by size: 4320 1396 1183 1175 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3389 4573 6892 7079 1700 Size: 110 1579 1184 2319 187 2869 Fragments arranged by size: 2869 2319 1579 1184 187 110 BsrGI T'GTAC_A Cuts at: 96 2599 3549 96 Size: 2503 950 4795 Fragments arranged by size: 4795 2503 950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8248 BssSI C'ACGA_G Cuts at: 6308 7320 6308 Size: 1012 7236 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8248 BstBI TT'CG_AA Cuts at: 7052 7052 Size: 8248 BstEII G'GTnAC_C Cuts at: 1184 3455 1184 Size: 2271 5977 BstXI CCAn_nnnn'nTGG Cuts at: 2371 2741 3857 2371 Size: 370 1116 6762 Fragments arranged by size: 6762 1116 370 BstYI r'GATC_y Cuts at: 1972 3821 4829 5578 5945 6330 6347 7799 7885 Size: 1849 1008 749 367 385 17 1452 86 Cuts at: 7885 7897 8243 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1849 1452 1008 749 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3763 4992 3763 Size: 1229 7019 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6757 Fragments arranged by size: 6757 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4647 487 Size: 683 56 3421 4088 Fragments arranged by size: 4088 3421 683 56 BtsI GCAGTG_nn' Cuts at: 2163 2268 3204 5764 6558 6578 2163 Size: 105 936 2560 794 20 3833 Fragments arranged by size: 3833 2560 936 794 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8248 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4351 4386 529 Size: 35 3787 35 4391 Fragments arranged by size: 4391 3787 35 35 DraI TTT'AAA Cuts at: 2076 3888 4156 4653 5552 2076 Size: 1812 268 497 899 4772 Fragments arranged by size: 4772 1812 899 497 268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7255 809 Size: 507 5939 1802 Fragments arranged by size: 5939 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3247 3391 4086 Size: 54 1425 50 275 18 1417 144 695 Cuts at: 4086 5695 6584 8213 8 Size: 1609 889 1629 43 Fragments arranged by size: 1629 1609 1425 1417 889 695 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5695 1537 Size: 275 3883 4090 Fragments arranged by size: 4090 3883 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3172 3574 4864 6178 1353 Size: 1819 402 1290 1314 3423 Fragments arranged by size: 3423 1819 1314 1290 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6835 7209 7355 387 Size: 1064 138 5246 374 146 1280 Fragments arranged by size: 5246 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5539 5539 Size: 8248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2400 3235 3363 3774 3833 4030 Size: 393 598 170 835 128 411 59 197 Cuts at: 4030 4126 4330 4694 4949 6311 6908 7695 7963 Size: 96 204 364 255 1362 597 787 268 Cuts at: 7963 1239 Size: 1524 Fragments arranged by size: 1524 1362 835 787 598 597 411 393 364 268 255 204 197 170 128 96 59 EcoO109I rG'GnC_Cy Cuts at: 2618 2746 3638 3864 2618 Size: 128 892 226 7002 Fragments arranged by size: 7002 892 226 128 EcoRI G'AATT_C Cuts at: 3748 5557 5935 3748 Size: 1809 378 6061 Fragments arranged by size: 6061 1809 378 EcoRV GAT'ATC Cuts at: 5071 5071 Size: 8248 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5491 5491 Size: 8248 FspI TGC'GCA Cuts at: 6754 6754 Size: 8248 HaeII r_GCGC'y Cuts at: 1678 2060 7395 1678 Size: 382 5335 2531 Fragments arranged by size: 5335 2531 382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2843 2875 5494 5526 6889 Size: 32 135 32 1271 32 2619 32 1363 Cuts at: 6889 6921 6963 6995 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2619 1363 1271 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 4012 4258 4473 4783 5592 Size: 988 281 322 1752 246 215 310 809 Cuts at: 5592 5838 669 Size: 246 3079 Fragments arranged by size: 3079 1752 988 809 322 310 281 246 246 215 HindIII A'AGCT_T Cuts at: 748 3978 748 Size: 3230 5018 HpaI GTT'AAC Cuts at: 4473 5838 4473 Size: 1365 6883 KpnI G_GTAC'C Cuts at: 5573 5573 Size: 8248 MfeI C'AATT_G Cuts at: 3675 5847 3675 Size: 2172 6076 MluI A'CGCG_T Cuts at: 8124 8124 Size: 8248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3035 4438 7362 7546 1305 Size: 1730 1403 2924 184 2007 Fragments arranged by size: 2924 2007 1730 1403 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3249 4088 10 Size: 54 1425 343 1417 839 4170 Fragments arranged by size: 4170 1425 1417 839 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2416 3453 3698 3908 6206 6565 6724 Size: 648 1250 1037 245 210 2298 359 159 Cuts at: 6724 518 Size: 2042 Fragments arranged by size: 2298 2042 1250 1037 648 359 245 210 159 MspA1I CmG'CkG Cuts at: 1606 2035 2041 4427 5294 5664 6341 7489 7734 Size: 429 6 2386 867 370 677 1148 245 Cuts at: 7734 1606 Size: 2120 Fragments arranged by size: 2386 2120 1148 867 677 429 370 245 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6757 Fragments arranged by size: 6757 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8248 NotI GC'GGCC_GC Cuts at: 5695 5695 Size: 8248 NspI r_CATG'y Cuts at: 1132 1229 2280 2397 4599 5606 7151 1132 Size: 97 1051 117 2202 1007 1545 2229 Fragments arranged by size: 2229 2202 1545 1051 1007 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8248 PciI A'CATG_T Cuts at: 2276 2393 7147 2276 Size: 117 4754 3377 Fragments arranged by size: 4754 3377 117 PflMI CCAn_nnn'nTGG Cuts at: 1108 5241 1108 Size: 4133 4115 PmeI GTTT'AAAC Cuts at: 5552 5552 Size: 8248 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6317 6349 7830 7862 6317 Size: 32 1481 32 6703 Fragments arranged by size: 6703 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2746 3864 2746 Size: 1118 7130 PshAI GACnn'nnGTC Cuts at: 1613 2460 3227 4284 1613 Size: 847 767 1057 5577 Fragments arranged by size: 5577 1057 847 767 PsiI TTA'TAA Cuts at: 2171 5408 5818 2171 Size: 3237 410 4601 Fragments arranged by size: 4601 3237 410 PstI C_TGCA'G Cuts at: 830 3291 4182 5156 5299 5600 830 Size: 2461 891 974 143 301 3478 Fragments arranged by size: 3478 2461 974 891 301 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8248 PvuII CAG'CTG Cuts at: 4427 5294 4427 Size: 867 7381 SacI G_AGCT'C Cuts at: 5541 5541 Size: 8248 SalI G'TCGA_C Cuts at: 1936 5590 1936 Size: 3654 4594 SbfI CC_TGCA'GG Cuts at: 5600 5600 Size: 8248 ScaI AGT'ACT Cuts at: 3854 6496 8208 3854 Size: 2642 1712 3894 Fragments arranged by size: 3894 2642 1712 SfcI C'TryA_G Cuts at: 826 1045 3287 3972 4178 5152 5167 5295 5596 Size: 219 2242 685 206 974 15 128 301 Cuts at: 5596 6731 7412 7603 826 Size: 1135 681 191 1471 Fragments arranged by size: 2242 1471 1135 974 685 681 301 219 206 191 128 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8248 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8248 SmaI CCC'GGG Cuts at: 1872 5575 1872 Size: 3703 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4431 4741 6352 7253 7515 820 Size: 197 926 2488 310 1611 901 262 1553 Fragments arranged by size: 2488 1611 1553 926 901 310 262 197 SnaBI TAC'GTA Cuts at: 493 6039 493 Size: 5546 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8248 SphI G_CATG'C Cuts at: 1132 5606 1132 Size: 4474 3774 SspI AAT'ATT Cuts at: 5 52 2121 6172 5 Size: 47 2069 4051 2081 Fragments arranged by size: 4051 2081 2069 47 StuI AGG'CCT Cuts at: 1517 2607 1517 Size: 1090 7158 StyI C'CwwG_G Cuts at: 513 1065 2004 2564 3867 4338 5687 513 Size: 552 939 560 1303 471 1349 3074 Fragments arranged by size: 3074 1349 1303 939 560 552 471 SwaI ATTT'AAAT Cuts at: 3888 3888 Size: 8248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3182 6275 6292 6445 6630 7075 325 Size: 1255 1602 3093 17 153 185 445 1498 Fragments arranged by size: 3093 1602 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2599 3549 3852 Size: 274 80 33 51 1443 622 950 303 Cuts at: 3852 5278 6494 8206 96 Size: 1426 1216 1712 138 Fragments arranged by size: 1712 1443 1426 1216 950 622 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2383 2415 8208 8240 2383 Size: 32 5793 32 2391 Fragments arranged by size: 5793 2391 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8248 XbaI T'CTAG_A Cuts at: 5584 5584 Size: 8248 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2184 4367 2184 Size: 2183 6065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8248 XmaI C'CCGG_G Cuts at: 1870 5573 1870 Size: 3703 4545 XmnI GAAnn'nnTTC Cuts at: 5161 6377 5161 Size: 1216 7032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6056 276 Size: 53 83 186 936 4522 2468 Fragments arranged by size: 4522 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI