Restriction map for
pFN21AB9464
(Linear) (Six-base) MAPSORT of: KIBB9464.seq Check: 9384 from: 1 to: 3570
KIBB9464.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AatII G_ACGT'C
Cuts at: 0 1260 3570
Size: 1260 2310
AccI GT'mk_AC
Cuts at: 0 1901 3570
Size: 1901 1669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1324 1520 1687 2678 3169 3570
Size: 364 960 196 167 991 491 401
Fragments arranged by size:
991 960 491 401 364 196 167
AflIII A'CryG_T
Cuts at: 0 1079 2545 3570
Size: 1079 1466 1025
Fragments arranged by size:
1466 1079 1025
AhdI GACnn_n'nnGTC
Cuts at: 0 2197 2458 2700 3570
Size: 2197 261 242 870
Fragments arranged by size:
2197 870 261 242
AleI CACnn'nnGTG
Cuts at: 0 2314 3570
Size: 2314 1256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3233 3570
Size: 3233 337
AlwNI CAG_nnn'CTG
Cuts at: 0 1665 1866 2299 3385 3507 3570
Size: 1665 201 433 1086 122 63
Fragments arranged by size:
1665 1086 433 201 122 63
ApaI G_GGCC'C
Cuts at: 0 552 819 914 3570
Size: 552 267 95 2656
Fragments arranged by size:
2656 552 267 95
ApaLI G'TGCA_C
Cuts at: 0 2549 3570
Size: 2549 1021
ApoI r'AATT_y
Cuts at: 0 2580 3570
Size: 2580 990
AvaI C'yCGr_G
Cuts at: 0 210 635 695 836 889 1473 3570
Size: 210 425 60 141 53 584 2097
Fragments arranged by size:
2097 584 425 210 141 60 53
AvrII C'CTAG_G
Cuts at: 0 895 3570
Size: 895 2675
BanI G'GyrC_C
Cuts at: 0 268 2788 3570
Size: 268 2520 782
Fragments arranged by size:
2520 782 268
BanII G_rGCy'C
Cuts at: 0 552 819 883 914 2913 3299 3570
Size: 552 267 64 31 1999 386 271
Fragments arranged by size:
1999 552 386 271 267 64 31
BbeI G_GCGC'C
Cuts at: 0 2792 3570
Size: 2792 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 1881 2117 2699 2774 3570
Size: 1881 236 582 75 796
Fragments arranged by size:
1881 796 582 236 75
BbvCI CC'TCA_GC
Cuts at: 0 571 3570
Size: 571 2999
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1572 1606 3570
Size: 1572 34 1964
Fragments arranged by size:
1964 1572 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3447 3570
Size: 3447 123
BclI T'GATC_A
Cuts at: 0 2520 2682 3570
Size: 2520 162 888
Fragments arranged by size:
2520 888 162
BglI GCCn_nnn'nGGC
Cuts at: 0 900 3570
Size: 900 2670
BglII A'GATC_T
Cuts at: 0 977 3570
Size: 977 2593
Bme1580I G_kGCm'C
Cuts at: 0 552 819 914 2553 3570
Size: 552 267 95 1639 1017
Fragments arranged by size:
1639 1017 552 267 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 2406 3570
Size: 2406 1164
BmtI G_CTAG'C
Cuts at: 0 2933 3570
Size: 2933 637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 682 1229 2076 2333 2433 3283 3570
Size: 622 60 547 847 257 100 850 287
Fragments arranged by size:
850 847 622 547 287 257 100 60
Bpu10I CC'TnA_GC
Cuts at: 0 571 3419 3570
Size: 571 2848 151
Fragments arranged by size:
2848 571 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1275 3158 3570
Size: 1036 239 1883 412
Fragments arranged by size:
1883 1036 412 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 8 632 964 1408 1649 2184 2658 3570
Size: 8 624 332 444 241 535 474 912
Fragments arranged by size:
912 624 535 474 444 332 241 8
BsaHI Gr'CG_yC
Cuts at: 0 534 1257 2789 3570
Size: 534 723 1532 781
Fragments arranged by size:
1532 781 723 534
BsaWI w'CCGG_w
Cuts at: 0 145 452 581 1568 2321 3570
Size: 145 307 129 987 753 1249
Fragments arranged by size:
1249 987 753 307 145 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1353 1383 1544 1574 2049 2079 2431 2461
Size: 1353 30 161 30 475 30 352 30
Cuts at: 2461 2630 2660 3478 3508 3570
Size: 169 30 818 30 62
Fragments arranged by size:
1353 818 475 352 169 161 62 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 26 486 1264 1445 2839 2844 2890 2934
Size: 26 460 778 181 1394 5 46 44
Cuts at: 2934 3098 3161 3199 3220 3499 3502 3505 3570
Size: 164 63 38 21 279 3 3 65
Fragments arranged by size:
1394 778 460 279 181 164 65 63
46 44 38 26 21 5 3 3
BseYI C'CCAG_C
Cuts at: 0 509 883 1449 1923 2035 2148 2893 2964
Size: 509 374 566 474 112 113 745 71
Cuts at: 2964 3395 3570
Size: 431 175
Fragments arranged by size:
745 566 509 474 431 374 175 113
112 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2887 3570
Size: 2887 683
BsiEI CG_ry'CG
Cuts at: 0 726 907 3570
Size: 726 181 2663
Fragments arranged by size:
2663 726 181
BsiHKAI G_wGCw'C
Cuts at: 0 209 866 2553 2913 3299 3570
Size: 209 657 1687 360 386 271
Fragments arranged by size:
1687 657 386 360 271 209
BsmI GAATG_Cn'
Cuts at: 0 1610 2066 2968 3293 3318 3570
Size: 1610 456 902 325 25 252
Fragments arranged by size:
1610 902 456 325 252 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1248 3570
Size: 1248 2322
Bsp1286I G_dGCh'C
Cuts at: 0 209 552 819 866 883 914 2553 2913
Size: 209 343 267 47 17 31 1639 360
Cuts at: 2913 3299 3570
Size: 386 271
Fragments arranged by size:
1639 386 360 343 271 267 209 47
31 17
BspEI T'CCGG_A
Cuts at: 0 145 452 3570
Size: 145 307 3118
Fragments arranged by size:
3118 307 145
BsrBI CCG'CTC
Cuts at: 0 546 564 606 666 786 833 2852 3570
Size: 546 18 42 60 120 47 2019 718
Fragments arranged by size:
2019 718 546 120 60 47 42 18
BsrDI GCAATG_nn'
Cuts at: 0 1590 2234 3570
Size: 1590 644 1336
Fragments arranged by size:
1590 1336 644
BsrFI r'CCGG_y
Cuts at: 0 3 476 2862 3570
Size: 3 473 2386 708
Fragments arranged by size:
2386 708 473 3
BssHII G'CGCG_C
Cuts at: 0 628 3570
Size: 628 2942
BssSI C'ACGA_G
Cuts at: 0 2872 2906 3402 3570
Size: 2872 34 496 168
Fragments arranged by size:
2872 496 168 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3313 3570
Size: 3313 257
BstEII G'GTnAC_C
Cuts at: 0 1749 3570
Size: 1749 1821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 2077 2316 3570
Size: 685 1392 239 1254
Fragments arranged by size:
1392 1254 685 239
BstYI r'GATC_y
Cuts at: 0 977 2296 3570
Size: 977 1319 1274
Fragments arranged by size:
1319 1274 977
Bsu36I CC'TnA_GG
Cuts at: 0 2095 3570
Size: 2095 1475
BtgI C'CryG_G
Cuts at: 0 18 275 489 588 648 720 2717 3570
Size: 18 257 214 99 60 72 1997 853
Fragments arranged by size:
1997 853 257 214 99 72 60 18
BtsI GCAGTG_nn'
Cuts at: 0 146 3207 3480 3570
Size: 146 3061 273 90
Fragments arranged by size:
3061 273 146 90
DraIII CAC_nnn'GTG
Cuts at: 0 2414 3570
Size: 2414 1156
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 3570
Size: 253 3317
EaeI y'GGCC_r
Cuts at: 0 371 591 651 723 904 2067 3537 3570
Size: 371 220 60 72 181 1163 1470 33
Fragments arranged by size:
1470 1163 371 220 181 72 60 33
EagI C'GGCC_G
Cuts at: 0 723 904 3570
Size: 723 181 2666
Fragments arranged by size:
2666 723 181
EarI CTCTTCn'nnn_
Cuts at: 0 68 944 3570
Size: 68 876 2626
Fragments arranged by size:
2626 876 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1645 3570
Size: 1645 1925
EcoICRI GAG'CTC
Cuts at: 0 2911 3297 3570
Size: 2911 386 273
Fragments arranged by size:
2911 386 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 622 682 1229 1324 1520 1687 2076
Size: 364 258 60 547 95 196 167 389
Cuts at: 2076 2333 2433 2678 3169 3283 3570
Size: 257 100 245 491 114 287
Fragments arranged by size:
547 491 389 364 287 258 257 245
196 167 114 100 95 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 400 3570
Size: 109 291 3170
Fragments arranged by size:
3170 291 109
EcoO109I rG'GnC_Cy
Cuts at: 0 353 567 609 669 815 841 910 921
Size: 353 214 42 60 146 26 69 11
Cuts at: 921 1622 1641 3570
Size: 701 19 1929
Fragments arranged by size:
1929 701 353 214 146 69 60 42
26 19 11
EcoRV GAT'ATC
Cuts at: 0 3348 3570
Size: 3348 222
HaeII r_GCGC'y
Cuts at: 0 2792 3570
Size: 2792 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1066 1098 1180 1212 1245 1277
Size: 817 32 217 32 82 32 33 32
Cuts at: 1277 1353 1385 1871 1903 2088 2120 2183 2215
Size: 76 32 486 32 185 32 63 32
Cuts at: 2215 2281 2313 2444 2476 2667 2686 2699 2718
Size: 66 32 131 32 191 19 13 19
Cuts at: 2718 3570
Size: 852
Fragments arranged by size:
852 817 486 217 191 185 131 82
76 66 63 33 32 32 32 32
32 32 32 32 32 32 19 19
13
HindIII A'AGCT_T
Cuts at: 0 2533 3570
Size: 2533 1037
KasI G'GCGC_C
Cuts at: 0 2788 3570
Size: 2788 782
MfeI C'AATT_G
Cuts at: 0 188 2423 3570
Size: 188 2235 1147
Fragments arranged by size:
2235 1147 188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1940 2512 2519 3570
Size: 1940 572 7 1051
Fragments arranged by size:
1940 1051 572 7
MscI TGG'CCA
Cuts at: 0 2069 3539 3570
Size: 2069 1470 31
Fragments arranged by size:
2069 1470 31
MslI CAynn'nnrTG
Cuts at: 0 274 2314 3570
Size: 274 2040 1256
Fragments arranged by size:
2040 1256 274
MspA1I CmG'CkG
Cuts at: 0 20 509 722 2122 2832 3507 3570
Size: 20 489 213 1400 710 675 63
Fragments arranged by size:
1400 710 675 489 213 63 20
NaeI GCC'GGC
Cuts at: 0 478 3570
Size: 478 3092
NarI GG'CG_CC
Cuts at: 0 2789 3570
Size: 2789 781
NcoI C'CATG_G
Cuts at: 0 275 489 2717 3570
Size: 275 214 2228 853
Fragments arranged by size:
2228 853 275 214
NgoMIV G'CCGG_C
Cuts at: 0 476 3570
Size: 476 3094
NheI G'CTAG_C
Cuts at: 0 2929 3570
Size: 2929 641
NspI r_CATG'y
Cuts at: 0 1083 2365 2703 3570
Size: 1083 1282 338 867
Fragments arranged by size:
1282 1083 867 338
PciI A'CATG_T
Cuts at: 0 1079 3570
Size: 1079 2491
PflMI CCAn_nnn'nTGG
Cuts at: 0 3385 3570
Size: 3385 185
PfoI T'CCnGG_A
Cuts at: 0 1348 3299 3570
Size: 1348 1951 271
Fragments arranged by size:
1951 1348 271
PpuMI rG'GwC_Cy
Cuts at: 0 567 841 921 1622 3570
Size: 567 274 80 701 1948
Fragments arranged by size:
1948 701 567 274 80
PsiI TTA'TAA
Cuts at: 0 1825 3570
Size: 1825 1745
PspOMI G'GGCC_C
Cuts at: 0 548 815 910 3570
Size: 548 267 95 2660
Fragments arranged by size:
2660 548 267 95
PvuII CAG'CTG
Cuts at: 0 509 2122 3507 3570
Size: 509 1613 1385 63
Fragments arranged by size:
1613 1385 509 63
SacI G_AGCT'C
Cuts at: 0 2913 3299 3570
Size: 2913 386 271
Fragments arranged by size:
2913 386 271
SacII CC_GC'GG
Cuts at: 0 21 723 3570
Size: 21 702 2847
Fragments arranged by size:
2847 702 21
SanDI GG'GwC_CC
Cuts at: 0 841 3570
Size: 841 2729
SapI GCTCTTCn'nnn_
Cuts at: 0 68 3570
Size: 68 3502
SfcI C'TryA_G
Cuts at: 0 462 792 1129 3570
Size: 462 330 337 2441
Fragments arranged by size:
2441 462 337 330
SfoI GGC'GCC
Cuts at: 0 2790 3570
Size: 2790 780
SmaI CCC'GGG
Cuts at: 0 212 697 3570
Size: 212 485 2873
Fragments arranged by size:
2873 485 212
SmlI C'TyrA_G
Cuts at: 0 1051 1290 3173 3570
Size: 1051 239 1883 397
Fragments arranged by size:
1883 1051 397 239
SphI G_CATG'C
Cuts at: 0 2365 3570
Size: 2365 1205
SspI AAT'ATT
Cuts at: 0 3251 3570
Size: 3251 319
StyI C'CwwG_G
Cuts at: 0 275 489 895 2717 2987 3570
Size: 275 214 406 1822 270 583
Fragments arranged by size:
1822 583 406 275 270 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1345 1380 2425 3570
Size: 1345 35 1045 1145
Fragments arranged by size:
1345 1145 1045 35
TatI w'GTAC_w
Cuts at: 0 92 803 3570
Size: 92 711 2767
Fragments arranged by size:
2767 711 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 1544 1576 2049 2081 3391 3423
Size: 125 32 1387 32 473 32 1310 32
Cuts at: 3423 3570
Size: 147
Fragments arranged by size:
1387 1310 473 147 125 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 172 393 3231 3570
Size: 172 221 2838 339
Fragments arranged by size:
2838 339 221 172
XmaI C'CCGG_G
Cuts at: 0 210 695 3570
Size: 210 485 2875
Fragments arranged by size:
2875 485 210
ZraI GAC'GTC
Cuts at: 0 1258 3570
Size: 1258 2312
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I HindIII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PflMI PfoI PpuMI PsiI PspOMI PvuII SacI
SacII SanDI SapI SfcI SfoI SmaI SmlI SphI
SspI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AloI AscI
AseI AsiSI BaeI BamHI BfrBI BlpI BmgBI BplI
BsaAI BsaBI BsiWI BspHI BspMI BsrGI BstBI BstZ17I
BtgZI ClaI CspCI DraI EcoRI FalI FseI FspI
FspAI HincII HpaI KpnI MluI NdeI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PspXI
PsrI PstI PvuI RsrII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
Tth111I XbaI XhoI XmnI