Restriction map for
pFN21AB9462
##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI AscI AseI AsnI Asp700I
AspEI AvrII BamHI BcgI BciVI
BglII BlnI BsaWI BseAI BsmBI
BspEI BspLU11I BsrDI BssHII BssSI
Bst1107I BstEII BstPI BstYI BstZ17I
Bsu36I CpoI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRV EcoT22I FseI
HpaI KspI MfeI MflI MluI
MroI MunI NdeI NheI NotI
NruI NsiI PacI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1640 GGTACC G^GTACC
AccI 2524 GTCTAC GT^[AC][GT]AC
AcsI 120 GAATTC [GA]^AATT[CT]
AcyI 304 GGCGCC G[GA]^CG[CT]C
ApaI 32 GGGCCC GGGCC^C
ApoI 120 GAATTC [GA]^AATT[CT]
Asp718I 1640 GGTACC G^GTACC
AspI 631 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1445 TGCGCA TGC^GCA
BbeI 304 GGCGCC GGCGC^C
BbrPI 2295 CACGTG CAC^GTG
BbsI 1590 GAAGAC GAAGAC(2/6)
BclI 1817 TGATCA T^GATCA
BfaI 1981 CTAG C^TAG
BpuAI 1590 GAAGAC GAAGAC(2/6)
BsaBI 1986 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 304 GGCGCC G[GA]^CG[CT]C
BsiWI 1020 CGTACG C^GTACG
BspDI 1228 ATCGAT AT^CGAT
BspHI 1925 TCATGA T^CATGA
BstBI 825 TTCGAA TT^CGAA
BstXI 485 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1228 ATCGAT AT^CGAT
DraII 2681 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 2170 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 2681 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 120 GAATTC G^AATTC
FbaI 1817 TGATCA T^GATCA
FspI 1445 TGCGCA TGC^GCA
HgaI 2460 GACGC GACGC(5/10)
Hin1I 304 GGCGCC G[GA]^CG[CT]C
HincII 1357 GTCAAC GT[CT]^[GA]AC
HindII 1357 GTCAAC GT[CT]^[GA]AC
HindIII 1738 AAGCTT A^AGCTT
KasI 304 GGCGCC G^GCGCC
KpnI 1640 GGTACC GGTAC^C
MaeI 1981 CTAG C^TAG
MamI 1986 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1718 GCCGGC GCC^GGC
NarI 304 GGCGCC GG^CGCC
NgoMIV 1718 GCCGGC G^CCGGC
NspV 825 TTCGAA TT^CGAA
PaeR7I 346 CTCGAG C^TCGAG
PflFI 631 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2478 GAGTC GAGTC(4/5)
PmaCI 2295 CACGTG CAC^GTG
PmlI 2295 CACGTG CAC^GTG
RcaI 1925 TCATGA T^CATGA
SexAI 1649 ACCAGGT A^CC[AT]GGT
SfoI 304 GGCGCC GGC^GCC
SfuI 825 TTCGAA TT^CGAA
Tth111I 631 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 346 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 208 CTTAAG C^TTAAG
1339 CTTAAG
Alw44I 1161 GTGCAC G^TGCAC
2298 GTGCAC
AlwI 999 GATCC GGATC(4/5)
258 GGATC
Aor51HI 2054 AGCGCT AGC^GCT
2688 AGCGCT
ApaLI 1161 GTGCAC G^TGCAC
2298 GTGCAC
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
982 GCTGAGG
BfrI 208 CTTAAG C^TTAAG
1339 CTTAAG
BglI 62 GCCTTTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2264 GCCTGCTGGGC
BlpI 1119 GCTGAGC GC^T[ACGT]AGC
1933 GCTGAGC
Bpu1102I 1119 GCTGAGC GC^T[ACGT]AGC
1933 GCTGAGC
BseRI 55 CTCCTC GAGGAG(10/8)
973 GAGGAG
BsmI 2504 GAATGC GAATGC(1/-1)
311 GCATTC
Bsp1407I 2372 TGTACA T^GTACA
2444 TGTACA
BsrGI 2372 TGTACA T^GTACA
2444 TGTACA
CelII 1119 GCTGAGC GC^T[ACGT]AGC
1933 GCTGAGC
EagI 1287 CGGCCG C^GGCCG
2432 CGGCCG
EclXI 1287 CGGCCG C^GGCCG
2432 CGGCCG
Eco47III 2054 AGCGCT AGC^GCT
2688 AGCGCT
Eco52I 1287 CGGCCG C^GGCCG
2432 CGGCCG
MseI 209 TTAA T^TAA
1340 TTAA
NcoI 623 CCATGG C^CATGG
1244 CCATGG
PshAI 1276 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2706 GACTTACGTC
PstI 1557 CTGCAG CTGCA^G
2125 CTGCAG
SacI 474 GAGCTC GAGCT^C
2662 GAGCTC
SphI 1604 GCATGC GCATG^C
2279 GCATGC
SspBI 2372 TGTACA T^GTACA
2444 TGTACA
Tru9I 209 TTAA T^TAA
1340 TTAA
Tsp509I 121 AATT ^AATT
1179 AATT
XcmI 1046 CCAGCCGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1802 CCACGTTGTTCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaAI BsaI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I