(Circular) (Six-base) MAPSORT of: pFN21AB9462.seq Check: 2836 from: 1 to: 7441 pFN21AB9462 7441 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2336 3507 3965 2336 Size: 1171 458 5812 Fragments arranged by size: 5812 1171 458 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5251 278 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4530 4784 1937 Size: 2593 254 4594 Fragments arranged by size: 4594 2593 254 Acc65I G'GTAC_C Cuts at: 3645 4762 3645 Size: 1117 6324 AclI AA'CG_TT Cuts at: 1563 5568 5941 1563 Size: 4005 373 3063 Fragments arranged by size: 4005 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 4099 5504 6888 7156 1632 Size: 825 1642 1405 1384 268 1917 Fragments arranged by size: 1917 1642 1405 1384 825 268 AfeI AGC'GCT Cuts at: 4061 4695 4061 Size: 634 6807 AflII C'TTAA_G Cuts at: 820 1017 2213 3344 820 Size: 197 1196 1131 4917 Fragments arranged by size: 4917 1196 1131 197 AflIII A'CryG_T Cuts at: 1203 2449 2610 3680 6340 7317 1203 Size: 1246 161 1070 2660 977 1327 Fragments arranged by size: 2660 1327 1246 1070 977 161 AgeI A'CCGG_T Cuts at: 6272 6272 Size: 7441 AhdI GACnn_n'nnGTC Cuts at: 6170 6170 Size: 7441 AleI CACnn'nnGTG Cuts at: 1166 3830 1166 Size: 2664 4777 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3356 1184 Size: 2172 5269 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2651 2683 2651 Size: 32 7409 AlwNI CAG_nnn'CTG Cuts at: 2362 3547 4138 5184 6756 2362 Size: 1185 591 1046 1572 3047 Fragments arranged by size: 3047 1572 1185 1046 591 ApaI G_GGCC'C Cuts at: 2041 2041 Size: 7441 ApaLI G'TGCA_C Cuts at: 3166 4303 5498 6654 3166 Size: 1137 1195 1156 3953 Fragments arranged by size: 3953 1195 1156 1137 ApoI r'AATT_y Cuts at: 1493 2125 4750 4976 5128 7237 1493 Size: 632 2625 226 152 2109 1697 Fragments arranged by size: 2625 2109 1697 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 5995 160 Size: 872 4963 1606 Fragments arranged by size: 4963 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 AvaI C'yCGr_G Cuts at: 1870 1943 2351 3865 4337 4766 1870 Size: 73 408 1514 472 429 4545 Fragments arranged by size: 4545 1514 472 429 408 73 BamHI G'GATC_C Cuts at: 4771 5138 4771 Size: 367 7074 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2309 2599 3332 3645 3953 Size: 325 217 627 522 290 733 313 308 Cuts at: 3953 4424 4762 6217 618 Size: 471 338 1455 1842 Fragments arranged by size: 1842 1455 733 627 522 471 338 325 313 308 290 217 BanII G_rGCy'C Cuts at: 1391 2041 2189 2483 2784 2936 2951 3306 3758 Size: 650 148 294 301 152 15 355 452 Cuts at: 3758 3945 4671 4760 1391 Size: 187 726 89 4072 Fragments arranged by size: 4072 726 650 452 355 301 294 187 152 148 89 15 BbeI G_GCGC'C Cuts at: 2313 2313 Size: 7441 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3602 928 Size: 971 1703 4767 Fragments arranged by size: 4767 1703 971 BbvCI CC'TCA_GC Cuts at: 2063 2988 2063 Size: 925 6516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5632 5666 1903 Size: 34 50 34 3611 34 3678 Fragments arranged by size: 3678 3611 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5333 6543 5333 Size: 1210 6231 BclI T'GATC_A Cuts at: 1548 3822 1548 Size: 2274 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2073 4275 6052 Size: 107 122 71 631 605 401 2202 1777 Cuts at: 6052 136 Size: 1525 Fragments arranged by size: 2202 1777 1525 631 605 401 122 107 71 BglII A'GATC_T Cuts at: 7436 7436 Size: 7441 BlpI GC'TnA_GC Cuts at: 3125 3939 4858 3125 Size: 814 919 5708 Fragments arranged by size: 5708 919 814 Bme1580I G_kGCm'C Cuts at: 1790 2041 2604 2650 3170 4075 4307 4338 4566 Size: 251 563 46 520 905 232 31 228 Cuts at: 4566 5502 6658 1790 Size: 936 1156 2573 Fragments arranged by size: 2573 1156 936 905 563 520 251 232 228 46 31 BmgBI CAC'GTC Cuts at: 1318 3157 3681 1318 Size: 1839 524 5078 Fragments arranged by size: 5078 1839 524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2165 2752 5172 Size: 416 53 83 399 24 748 587 2420 Cuts at: 5172 6130 442 Size: 958 1753 Fragments arranged by size: 2420 1753 958 748 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7441 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3772 3772 Size: 7441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2317 2843 2906 2996 3034 6101 1239 Size: 1022 56 526 63 90 38 3067 2579 Fragments arranged by size: 3067 2579 1022 526 90 63 56 38 Bpu10I CC'TnA_GC Cuts at: 2063 2988 2063 Size: 925 6516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3125 3767 4220 4703 5566 6431 6729 2636 Size: 489 642 453 483 863 865 298 3348 Fragments arranged by size: 3348 865 863 642 489 483 453 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 4334 4480 6104 882 Size: 617 992 1843 146 1624 2219 Fragments arranged by size: 2219 1843 1624 992 617 146 BsaAI yAC'GTr Cuts at: 493 1204 2613 3886 4302 5232 493 Size: 711 1409 1273 416 930 2702 Fragments arranged by size: 2702 1409 1273 930 711 416 BsaBI GATnn'nnATC Cuts at: 3995 7435 3995 Size: 3440 4001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 5248 5630 6265 Size: 53 83 186 936 777 2938 382 635 Cuts at: 6265 275 Size: 1451 Fragments arranged by size: 2938 1451 936 777 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5874 6272 6546 6693 7104 5874 Size: 398 274 147 411 6211 Fragments arranged by size: 6211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2573 2603 1178 Size: 30 165 30 912 30 228 30 6016 Fragments arranged by size: 6016 912 228 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2993 1186 Size: 865 942 5634 Fragments arranged by size: 5634 942 865 BseYI C'CCAG_C Cuts at: 2305 2943 3336 4138 4273 4690 6644 7140 2305 Size: 638 393 802 135 417 1954 496 2606 Fragments arranged by size: 2606 1954 802 638 496 417 393 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2393 2954 3242 3248 3485 1163 Size: 573 657 561 288 6 237 5119 Fragments arranged by size: 5119 657 573 561 288 237 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3295 4440 4891 5652 6680 Size: 275 186 1039 255 1145 451 761 1028 Cuts at: 6680 1540 Size: 2301 Fragments arranged by size: 2301 1145 1039 1028 761 451 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2483 3170 4236 4307 4671 5502 5587 6658 2483 Size: 687 1066 71 364 831 85 1071 3266 Fragments arranged by size: 3266 1071 1066 831 687 364 85 71 BsiWI C'GTAC_G Cuts at: 3025 3025 Size: 7441 BsmI GAATG_Cn' Cuts at: 2316 4515 4952 5045 2316 Size: 2199 437 93 4712 Fragments arranged by size: 4712 2199 437 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2189 2483 2604 2650 2784 2936 Size: 399 251 148 294 121 46 134 152 Cuts at: 2936 2951 3170 3306 3758 3945 4075 4236 4307 Size: 15 219 136 452 187 130 161 71 Cuts at: 4307 4338 4566 4671 4760 5502 5587 6658 1391 Size: 31 228 105 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 452 399 294 251 228 219 187 161 152 148 136 134 130 121 105 89 85 71 46 31 15 BspEI T'CCGG_A Cuts at: 7104 7104 Size: 7441 BspHI T'CATG_A Cuts at: 3930 5330 7060 3930 Size: 1400 1730 4311 Fragments arranged by size: 4311 1730 1400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 2336 3507 3965 4796 844 Size: 1451 41 1171 458 831 3489 Fragments arranged by size: 3489 1451 1171 831 458 41 BsrBI CCG'CTC Cuts at: 1394 1823 2703 3517 3537 3555 3589 5328 1394 Size: 429 880 814 20 18 34 1739 3507 Fragments arranged by size: 3507 1739 880 814 429 34 20 18 BsrDI GCAATG_nn' Cuts at: 65 1248 5936 6110 65 Size: 1183 4688 174 1396 Fragments arranged by size: 4688 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2235 3072 3723 6085 6272 1700 Size: 110 425 837 651 2362 187 2869 Fragments arranged by size: 2869 2362 837 651 425 187 110 BsrGI T'GTAC_A Cuts at: 96 4377 4449 96 Size: 4281 72 3088 Fragments arranged by size: 4281 3088 72 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7441 BssSI C'ACGA_G Cuts at: 5501 6513 5501 Size: 1012 6429 BstAPI GCAn_nnn'nTGC Cuts at: 794 3271 3547 3615 3720 4561 794 Size: 2477 276 68 105 841 3674 Fragments arranged by size: 3674 2477 841 276 105 68 BstBI TT'CG_AA Cuts at: 2831 6245 2831 Size: 3414 4027 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7441 BstXI CCAn_nnnn'nTGG Cuts at: 2497 2497 Size: 7441 BstYI r'GATC_y Cuts at: 1972 4771 5138 5523 5540 6992 7078 7090 7436 Size: 2799 367 385 17 1452 86 12 346 Cuts at: 7436 1972 Size: 1977 Fragments arranged by size: 2799 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2628 2774 3249 3789 513 Size: 552 1563 146 475 540 4165 Fragments arranged by size: 4165 1563 552 540 475 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2737 3361 3784 487 Size: 683 56 1511 624 423 4144 Fragments arranged by size: 4144 1511 683 624 423 56 BtsI GCAGTG_nn' Cuts at: 2422 3115 3625 4957 5751 5771 2422 Size: 693 510 1332 794 20 4092 Fragments arranged by size: 4092 1332 794 693 510 20 ClaI AT'CG_AT Cuts at: 1554 3234 1554 Size: 1680 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2185 2220 529 Size: 35 1621 35 5750 Fragments arranged by size: 5750 1621 35 35 DraI TTT'AAA Cuts at: 4745 4745 Size: 7441 DraIII CAC_nnn'GTG Cuts at: 4180 4180 Size: 7441 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6448 809 Size: 507 5132 1802 Fragments arranged by size: 5132 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2179 2499 3292 Size: 54 1425 50 275 18 349 320 793 Cuts at: 3292 3527 3786 4104 4437 4602 4698 4888 5777 Size: 235 259 318 333 165 96 190 889 Cuts at: 5777 7406 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 793 349 333 320 318 275 259 235 190 165 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 4437 4888 1537 Size: 275 1480 1145 451 4090 Fragments arranged by size: 4090 1480 1145 451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2009 2522 3785 4547 5371 1353 Size: 656 513 1263 762 824 3423 Fragments arranged by size: 3423 1263 824 762 656 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2801 6028 6402 6548 387 Size: 1064 138 1212 3227 374 146 1280 Fragments arranged by size: 3227 1280 1212 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2481 4669 2481 Size: 2188 5253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2317 2457 2843 2906 2996 3034 Size: 393 629 56 140 386 63 90 38 Cuts at: 3034 4099 5504 6101 6888 7156 1239 Size: 1065 1405 597 787 268 1524 Fragments arranged by size: 1524 1405 1065 787 629 597 393 386 268 140 90 63 56 38 EcoNI CCTnn'n_nnAGG Cuts at: 2332 2978 3143 3639 2332 Size: 646 165 496 6134 Fragments arranged by size: 6134 646 496 165 EcoO109I rG'GnC_Cy Cuts at: 4687 4687 Size: 7441 EcoRI G'AATT_C Cuts at: 2125 4750 5128 2125 Size: 2625 378 4438 Fragments arranged by size: 4438 2625 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2038 4654 2038 Size: 2616 4825 FspI TGC'GCA Cuts at: 3452 5947 3452 Size: 2495 4946 HaeII r_GCGC'y Cuts at: 1678 2313 2381 2555 3427 4063 4494 4697 6588 Size: 635 68 174 872 636 431 203 1891 Cuts at: 6588 1678 Size: 2531 Fragments arranged by size: 2531 1891 872 636 635 431 203 174 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2573 2605 4646 Size: 32 135 32 743 32 226 32 2041 Cuts at: 4646 4678 6082 6114 6156 6188 1373 Size: 32 1404 32 42 32 2626 Fragments arranged by size: 2626 2041 1404 743 226 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3364 4785 5031 669 Size: 988 281 1426 1421 246 3079 Fragments arranged by size: 3079 1426 1421 988 281 246 HindIII A'AGCT_T Cuts at: 748 3743 748 Size: 2995 4446 HpaI GTT'AAC Cuts at: 5031 5031 Size: 7441 KasI G'GCGC_C Cuts at: 2309 2309 Size: 7441 KpnI G_GTAC'C Cuts at: 3649 4766 3649 Size: 1117 6324 MfeI C'AATT_G Cuts at: 5040 5040 Size: 7441 MluI A'CGCG_T Cuts at: 7317 7317 Size: 7441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 6555 6739 1305 Size: 3333 1917 184 2007 Fragments arranged by size: 3333 2007 1917 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2501 4106 4700 10 Size: 54 1425 343 669 1605 594 2751 Fragments arranged by size: 2751 1605 1425 669 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2495 3830 4148 5399 5758 5917 518 Size: 648 1329 1335 318 1251 359 159 2042 Fragments arranged by size: 2042 1335 1329 1251 648 359 318 159 MspA1I CmG'CkG Cuts at: 1606 2409 2943 3340 3448 3547 4102 4135 4201 Size: 803 534 397 108 99 555 33 66 Cuts at: 4201 4857 5534 6682 6927 1606 Size: 656 677 1148 245 2120 Fragments arranged by size: 2120 1148 803 677 656 555 534 397 245 108 99 66 33 NaeI GCC'GGC Cuts at: 3725 3725 Size: 7441 NarI GG'CG_CC Cuts at: 2310 2310 Size: 7441 NcoI C'CATG_G Cuts at: 513 1065 2628 3249 513 Size: 552 1563 621 4705 Fragments arranged by size: 4705 1563 621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7441 NgoMIV G'CCGG_C Cuts at: 3723 3723 Size: 7441 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7441 NotI GC'GGCC_GC Cuts at: 4888 4888 Size: 7441 NspI r_CATG'y Cuts at: 1132 1229 3613 3697 4288 4522 4799 6344 1132 Size: 97 2384 84 591 234 277 1545 2229 Fragments arranged by size: 2384 2229 1545 591 277 234 97 84 PasI CC'CwG_GG Cuts at: 1348 2095 3762 4504 1348 Size: 747 1667 742 4285 Fragments arranged by size: 4285 1667 747 742 PciI A'CATG_T Cuts at: 6340 6340 Size: 7441 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2629 4180 1108 Size: 1131 390 1551 4369 Fragments arranged by size: 4369 1551 1131 390 PfoI T'CCnGG_A Cuts at: 2820 3485 4191 2820 Size: 665 706 6070 Fragments arranged by size: 6070 706 665 PmeI GTTT'AAAC Cuts at: 4745 4745 Size: 7441 PmlI CAC'GTG Cuts at: 4302 4302 Size: 7441 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5510 5542 7023 7055 5510 Size: 32 1481 32 5896 Fragments arranged by size: 5896 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3285 4715 1613 Size: 1672 1430 4339 Fragments arranged by size: 4339 1672 1430 PsiI TTA'TAA Cuts at: 5011 5011 Size: 7441 PspOMI G'GGCC_C Cuts at: 2037 2037 Size: 7441 PspXI vC'TCGA_Gb Cuts at: 2351 2351 Size: 7441 PstI C_TGCA'G Cuts at: 830 3566 4134 4793 830 Size: 2736 568 659 3478 Fragments arranged by size: 3478 2736 659 568 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7441 PvuII CAG'CTG Cuts at: 2943 3340 3448 3547 4102 4135 2943 Size: 397 108 99 555 33 6249 Fragments arranged by size: 6249 555 397 108 99 33 SacI G_AGCT'C Cuts at: 2483 4671 2483 Size: 2188 5253 SalI G'TCGA_C Cuts at: 1936 4783 1936 Size: 2847 4594 SbfI CC_TGCA'GG Cuts at: 4793 4793 Size: 7441 ScaI AGT'ACT Cuts at: 5689 7401 5689 Size: 1712 5729 SexAI A'CCwGG_T Cuts at: 3654 3654 Size: 7441 SfcI C'TryA_G Cuts at: 826 1045 3324 3562 4130 4279 4789 5924 6605 Size: 219 2279 238 568 149 510 1135 681 Cuts at: 6605 6796 826 Size: 191 1471 Fragments arranged by size: 2279 1471 1135 681 568 510 238 219 191 149 SfoI GGC'GCC Cuts at: 2311 2311 Size: 7441 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7441 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7441 SmaI CCC'GGG Cuts at: 1872 4768 1872 Size: 2896 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 2351 2651 3140 3344 3746 Size: 197 926 270 138 300 489 204 402 Cuts at: 3746 4235 4682 5545 6446 6708 820 Size: 489 447 863 901 262 1553 Fragments arranged by size: 1553 926 901 863 489 489 447 402 300 270 262 204 197 138 SnaBI TAC'GTA Cuts at: 493 5232 493 Size: 4739 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7441 SphI G_CATG'C Cuts at: 1132 3613 4288 4799 1132 Size: 2481 675 511 3774 Fragments arranged by size: 3774 2481 675 511 SspI AAT'ATT Cuts at: 5 52 5365 5 Size: 47 5313 2081 Fragments arranged by size: 5313 2081 47 StuI AGG'CCT Cuts at: 1517 2163 2714 3863 1517 Size: 646 551 1149 5095 Fragments arranged by size: 5095 1149 646 551 StyI C'CwwG_G Cuts at: 513 1065 2628 2784 3249 3949 4880 513 Size: 552 1563 156 465 700 931 3074 Fragments arranged by size: 3074 1563 931 700 552 465 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 2443 2447 2473 3350 4054 5468 Size: 1255 777 86 4 26 877 704 1414 Cuts at: 5468 5485 5638 5823 6268 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1414 1255 877 777 704 445 185 153 86 26 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3348 3468 4377 Size: 274 80 33 51 1443 1371 120 909 Cuts at: 4377 4449 5687 7399 96 Size: 72 1238 1712 138 Fragments arranged by size: 1712 1443 1371 1238 909 274 138 120 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 7401 7433 2111 Size: 32 5258 32 2119 Fragments arranged by size: 5258 2119 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2639 1316 Size: 1323 6118 XbaI T'CTAG_A Cuts at: 4777 4777 Size: 7441 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3814 3058 Size: 756 6685 XhoI C'TCGA_G Cuts at: 1943 2351 1943 Size: 408 7033 XmaI C'CCGG_G Cuts at: 1870 4766 1870 Size: 2896 4545 XmnI GAAnn'nnTTC Cuts at: 5570 5570 Size: 7441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5249 276 Size: 53 83 186 936 3715 2468 Fragments arranged by size: 3715 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI