Restriction map for pFN21AB9454
 (Circular) (Six-base) MAPSORT of: pFN21AB9454.seq  Check: 9407  from: 1  to: 6262



pFN21AB9454  6262 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4072     278 
   Size:         53      83     186     936    2536    2468

  Fragments arranged by size: 

                2536    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2055    3605    1937 
   Size:        118    1550    4594

  Fragments arranged by size: 

                4594    1550     118
 
Acc65I G'GTAC_C

Cuts at:   3583    3583 
   Size:       6262
 
AclI AA'CG_TT

Cuts at:   1563    4389    4762    1563 
   Size:       2826     373    3063

  Fragments arranged by size: 

                3063    2826     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2096    2802    4325    5709    5977    1632 
   Size:        464     706    1523    1384     268    1917

  Fragments arranged by size: 

                1917    1523    1384     706     464     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6065
 
AflIII A'CryG_T

Cuts at:   1203    3074    5161    6138    1203 
   Size:       1871    2087     977    1327

  Fragments arranged by size: 

                2087    1871    1327     977
 
AgeI A'CCGG_T

Cuts at:   5093    5093 
   Size:       6262
 
AhdI GACnn_n'nnGTC

Cuts at:   4991    4991 
   Size:       6262
 
AleI CACnn'nnGTG

Cuts at:   1166    3029    1166 
   Size:       1863    4399
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3329    3406    1184 
   Size:       2145      77    4040

  Fragments arranged by size: 

                4040    2145      77
 
AlwNI CAG_nnn'CTG

Cuts at:   4005    5577    4005 
   Size:       1572    4690
 
ApaLI G'TGCA_C

Cuts at:   4319    5475    4319 
   Size:       1156    5106
 
ApoI r'AATT_y

Cuts at:   1493    2455    3571    3797    3949    6058    1493 
   Size:        962    1116     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1116     962     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4816     160 
   Size:        872    3784    1606

  Fragments arranged by size: 

                3784    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6262
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3587    1870 
   Size:         73    1644    4545

  Fragments arranged by size: 

                4545    1644      73
 
BamHI G'GATC_C

Cuts at:   3592    3959    3592 
   Size:        367    5895
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3583    5038     618 
   Size:        325     217     627    1796    1455    1842

  Fragments arranged by size: 

                1842    1796    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2916    3367    3581    1391 
   Size:       1525     451     214    4072

  Fragments arranged by size: 

                4072    1525     451     214
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2115    3071     928 
   Size:        971     216     956    4119

  Fragments arranged by size: 

                4119     971     956     216
 
BbvCI CC'TCA_GC

Cuts at:   2790    2790 
   Size:       6262
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4453    4487    1903 
   Size:         34    2516      34    3678

  Fragments arranged by size: 

                3678    2516      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4154    5364    4154 
   Size:       1210    5052
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6262
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4873     136 
   Size:        107     122      71     631     605    3201    1525

  Fragments arranged by size: 

                3201    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   3310    3475    6257    3310 
   Size:        165    2782    3315

  Fragments arranged by size: 

                3315    2782     165
 
BlpI GC'TnA_GC

Cuts at:   3679    3679 
   Size:       6262
 
Bme1580I G_kGCm'C

Cuts at:   1790    4323    5479    1790 
   Size:       2533    1156    2573

  Fragments arranged by size: 

                2573    2533    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6262
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2726    2960    3993 
   Size:        416      53      83     399      24    1309     234    1033

Cuts at:   3993    4951     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1309    1033     958     416     399     234      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2619    2781    4922    1239 
   Size:       1380     162    2141    2579

  Fragments arranged by size: 

                2579    2141    1380     162
 
Bpu10I CC'TnA_GC

Cuts at:   2657    2790    2657 
   Size:        133    6129
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3093    3130    4387    5252    5550    3093 
   Size:         37    1257     865     298    3805

  Fragments arranged by size: 

                3805    1257     865     298      37
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2826    4925     882 
   Size:        617    1327    2099    2219

  Fragments arranged by size: 

                2219    2099    1327     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3235    4053     493 
   Size:        711    2031     818    2702

  Fragments arranged by size: 

                2702    2031     818     711
 
BsaBI GATnn'nnATC

Cuts at:   6256    6256 
   Size:       6262
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4069    4451    5086     275 
   Size:         53      83     186     936    2536     382     635    1451

  Fragments arranged by size: 

                2536    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2011    4695    5093    5367    5514    5925    2011 
   Size:       2684     398     274     147     411    2348

  Fragments arranged by size: 

                2684    2348     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2592    2622    2754    2784    2823 
   Size:         30     165      30    1189      30     132      30      39

Cuts at:   2823    2853    1178 
   Size:         30    4587

  Fragments arranged by size: 

                4587    1189     165     132      39      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2341    2359    2717    1186 
   Size:       1155      18     358    4731

  Fragments arranged by size: 

                4731    1155     358      18
 
BseYI C'CCAG_C

Cuts at:   3499    5465    5961    3499 
   Size:       1966     496    3800

  Fragments arranged by size: 

                3800    1966     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2691    1163 
   Size:        573     955    4734

  Fragments arranged by size: 

                4734     955     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3712    4473    5501    1540 
   Size:        275     186    1711     761    1028    2301

  Fragments arranged by size: 

                2301    1711    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2775    3367    4323    4408    5479    2775 
   Size:        592     956      85    1071    3558

  Fragments arranged by size: 

                3558    1071     956     592      85
 
BsmI GAATG_Cn'

Cuts at:   3008    3773    3866    3008 
   Size:        765      93    5404

  Fragments arranged by size: 

                5404     765      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2775    2916    3367    3581    4323    4408    5479 
   Size:        399     985     141     451     214     742      85    1071

Cuts at:   5479    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     985     742     451     399     214     141
                  85
 
BspEI T'CCGG_A

Cuts at:   5925    5925 
   Size:       6262
 
BspHI T'CATG_A

Cuts at:   4151    5881    4151 
   Size:       1730    4532
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2663    3617     844 
   Size:       1819     954    3489

  Fragments arranged by size: 

                3489    1819     954
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4149    1394 
   Size:        429    2326    3507

  Fragments arranged by size: 

                3507    2326     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4757    4931      65 
   Size:       1183    3509     174    1396

  Fragments arranged by size: 

                3509    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4906    5093    1700 
   Size:        110    3096     187    2869

  Fragments arranged by size: 

                3096    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2512      96 
   Size:       2416    3846
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6262
 
BssSI C'ACGA_G

Cuts at:   4322    5334    4322 
   Size:       1012    5250
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6262
 
BstBI TT'CG_AA

Cuts at:   5066    5066 
   Size:       6262
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6262
 
BstYI r'GATC_y

Cuts at:   1972    3310    3475    3592    3959    4344    4361    5813    5899 
   Size:       1338     165     117     367     385      17    1452      86

Cuts at:   5899    5911    6257    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452    1338     385     367     346     165     117
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5710
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2887     487 
   Size:        683      56    1661    3862

  Fragments arranged by size: 

                3862    1661     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3514    3778    4572    4592    3514 
   Size:        264     794      20    5184

  Fragments arranged by size: 

                5184     794     264      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6262
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6227
 
DraI TTT'AAA

Cuts at:   2095    3566    2095 
   Size:       1471    4791
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3467    5269     809 
   Size:        507    2151    1802    1802

  Fragments arranged by size: 

                2151    1802    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3709    4598    6227 
   Size:         54    1425      50     275      18    1879     889    1629

Cuts at:   6227       8 
   Size:         43

  Fragments arranged by size: 

                1879    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3709    1537 
   Size:        275    1897    4090

  Fragments arranged by size: 

                4090    1897     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2341    2359    2368    2377    2386    2392    2410    2588 
   Size:        988      18       9       9       9       6      18     178

Cuts at:   2588    4192    1353 
   Size:       1604    3423

  Fragments arranged by size: 

                3423    1604     988     178      18      18       9       9
                   9       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2218    4849    5223    5369     387 
   Size:       1064     138     629    2631     374     146    1280

  Fragments arranged by size: 

                2631    1280    1064     629     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3365    3365 
   Size:       6262
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2096    2619    2781    2802    4325    4922    5709 
   Size:        393     464     523     162      21    1523     597     787

Cuts at:   5709    5977    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1523     787     597     523     464     393     268
                 162      21
 
EcoRI G'AATT_C

Cuts at:   3571    3949    3571 
   Size:        378    5884
 
FspI TGC'GCA

Cuts at:   4768    4768 
   Size:       6262
 
HaeII r_GCGC'y

Cuts at:   1678    5409    1678 
   Size:       3731    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2121    2153    2318    2350    2823 
   Size:         32     135      32     549      32     165      32     473

Cuts at:   2823    2855    4903    4935    4977    5009    1373 
   Size:         32    2048      32      42      32    2626

  Fragments arranged by size: 

                2626    2048     549     473     165     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3606    3852     669 
   Size:        988     281    1668     246    3079

  Fragments arranged by size: 

                3079    1668     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6262
 
HpaI GTT'AAC

Cuts at:   3852    3852 
   Size:       6262
 
KpnI G_GTAC'C

Cuts at:   3587    3587 
   Size:       6262
 
MfeI C'AATT_G

Cuts at:   3861    3861 
   Size:       6262
 
MluI A'CGCG_T

Cuts at:   6138    6138 
   Size:       6262
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5376    5560    1305 
   Size:       4071     184    2007

  Fragments arranged by size: 

                4071    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4440

  Fragments arranged by size: 

                4440    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2103    3029    4220    4579    4738     518 
   Size:        648     937     926    1191     359     159    2042

  Fragments arranged by size: 

                2042    1191     937     926     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3678    4355    5503    5748    1606 
   Size:       2072     677    1148     245    2120

  Fragments arranged by size: 

                2120    2072    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5710
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6262
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6262
 
NotI GC'GGCC_GC

Cuts at:   3709    3709 
   Size:       6262
 
NspI r_CATG'y

Cuts at:   1132    1229    3078    3620    5165    1132 
   Size:         97    1849     542    1545    2229

  Fragments arranged by size: 

                2229    1849    1545     542      97
 
PasI CC'CwG_GG

Cuts at:   1348    3535    1348 
   Size:       2187    4075
 
PciI A'CATG_T

Cuts at:   3074    5161    3074 
   Size:       2087    4175
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6262
 
PfoI T'CCnGG_A

Cuts at:   2596    2596 
   Size:       6262
 
PmeI GTTT'AAAC

Cuts at:   3566    3566 
   Size:       6262
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4331    4363    5844    5876    4331 
   Size:         32    1481      32    4717

  Fragments arranged by size: 

                4717    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    2051    3335    1613 
   Size:        438    1284    4540

  Fragments arranged by size: 

                4540    1284     438
 
PsiI TTA'TAA

Cuts at:   3832    3832 
   Size:       6262
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   2415    2447    2415 
   Size:         32    6230
 
PstI C_TGCA'G

Cuts at:    830    3614     830 
   Size:       2784    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6262
 
SacI G_AGCT'C

Cuts at:   3367    3367 
   Size:       6262
 
SalI G'TCGA_C

Cuts at:   1936    3604    1936 
   Size:       1668    4594
 
SbfI CC_TGCA'GG

Cuts at:   3614    3614 
   Size:       6262
 
ScaI AGT'ACT

Cuts at:   4510    6222    4510 
   Size:       1712    4550
 
SfcI C'TryA_G

Cuts at:    826    1045    2849    3043    3610    4745    5426    5617     826 
   Size:        219    1804     194     567    1135     681     191    1471

  Fragments arranged by size: 

                1804    1471    1135     681     567     219     194     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6262
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6262
 
SmaI CCC'GGG

Cuts at:   1872    3589    1872 
   Size:       1717    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3108    3145    4366    5267    5529     820 
   Size:        197     926    1165      37    1221     901     262    1553

  Fragments arranged by size: 

                1553    1221    1165     926     901     262     197      37
            
 
SnaBI TAC'GTA

Cuts at:    493    3235    4053     493 
   Size:       2742     818    2702

  Fragments arranged by size: 

                2742    2702     818
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6262
 
SphI G_CATG'C

Cuts at:   1132    3620    1132 
   Size:       2488    3774
 
SspI AAT'ATT

Cuts at:      5      52    4186       5 
   Size:         47    4134    2081

  Fragments arranged by size: 

                4134    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2820    1517 
   Size:       1303    4959
 
StyI C'CwwG_G

Cuts at:    513    1065    2284    3701     513 
   Size:        552    1219    1417    3074

  Fragments arranged by size: 

                3074    1417    1219     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2027    2078    2858    3225    3491    4289    4306 
   Size:       1255     447      51     780     367     266     798      17

Cuts at:   4306    4459    4644    5089     325 
   Size:        153     185     445    1498

  Fragments arranged by size: 

                1498    1255     798     780     447     445     367     266
                 185     153      51      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2512    3511    4508 
   Size:        274      80      33      51    1443     535     999     997

Cuts at:   4508    6220      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443     999     997     535     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2653    2685    2754    2786    6222    6254    2653 
   Size:         32      69      32    3436      32    2661

  Fragments arranged by size: 

                3436    2661      69      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2837    1316 
   Size:       1521    4741
 
XbaI T'CTAG_A

Cuts at:   3598    3598 
   Size:       6262
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3532    3532 
   Size:       6262
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6262
 
XmaI C'CCGG_G

Cuts at:   1870    3587    1870 
   Size:       1717    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2553    4391    2553 
   Size:       1838    4424
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4070     276 
   Size:         53      83     186     936    2536    2468

  Fragments arranged by size: 

                2536    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PsrI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI
    PspXI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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