Restriction map for
pFN21AB9443
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 60 CGCG CG^CG
AvaI 342 CTCGGG C^[CT]CG[GA]G
AviII 533 TGCGCA TGC^GCA
AvrII 906 CCTAGG C^CTAGG
BfaI 907 CTAG C^TAG
BglI 169 GCCGTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 719 AGATCT A^GATCT
BlnI 906 CCTAGG C^CTAGG
BpmI 664 CTCCAG CTGGAG(16/14)
BsaWI 32 ACCGGA [AT]^CCGG[AT]
BsiEI 83 CGGCCG CG[GA][CT]^CG
BsmAI 21 GTCTC GTCTC(1/5)
BsoBI 342 CTCGGG C^[CT]CG[GA]G
BspHI 800 TCATGA T^CATGA
BsrI 443 CCAGT ACTGG(1/-1)
BssSI 658 CACGAG CACGAG(-5/-1)
BstUI 60 CGCG CG^CG
BstYI 719 AGATCT [GA]^GATC[CT]
DdeI 369 CTCAG C^T[ACGT]AG
EagI 83 CGGCCG C^GGCCG
EarI 156 GAAGAG CTCTTC(1/4)
EclXI 83 CGGCCG C^GGCCG
Eco52I 83 CGGCCG C^GGCCG
FauI 729 CCCGC CCCGC(4/6)
FspI 533 TGCGCA TGC^GCA
HaeII 125 AGCGCC [GA]GCGC^[CT]
HinfI 161 GAATC G^A[ACGT]TC
Ksp632I 156 GAAGAG CTCTTC(1/4)
MaeI 907 CTAG C^TAG
MaeII 599 ACGT A^CGT
MaeIII 397 GTCAC ^GT[ACGT]AC
MflI 719 AGATCT [GA]^GATC[CT]
MslI 138 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 60 CGCG CG^CG
NaeI 591 GCCGGC GCC^GGC
NgoMIV 591 GCCGGC G^CCGGC
RcaI 800 TCATGA T^CATGA
SfaNI 813 GATGC GCATC(5/9)
SfiI 168 GGCCGTGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 481 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 733 CTCAAG C^T[CT][GA]AG
TfiI 161 GAATC G^A[AT]TC
Tsp45I 397 GTCAC ^GT[GC]AC
TspRI 444 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 719 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 411 GACGCC G[GA]^CG[CT]C
435 GGCGTC
AfaI 88 GTAC GT^AC
690 GTAC
AlwI 654 GGATC GGATC(4/5)
915 GGATC
BbsI 604 GTCTTC GAAGAC(2/6)
853 GTCTTC
BcnI 327 CCCGG CC^[GC]GG
356 CCGGG
BpuAI 604 GTCTTC GAAGAC(2/6)
853 GTCTTC
BsaHI 411 GACGCC G[GA]^CG[CT]C
435 GGCGTC
BsgI 320 CTGCAC GTGCAG(16/14)
202 GTGCAG
BstF5I 54 GGATG GGATG(2/0)
627 GGATG
DsaI 68 CCGTGG C^C[GA][CT]GG
512 CCACGG
FokI 54 GGATG GGATG(9/13)
627 GGATG
HgaI 411 GACGC GACGC(5/10)
436 GCGTC
Hin1I 411 GACGCC G[GA]^CG[CT]C
435 GGCGTC
NciI 327 CCCGG CC^[GC]GG
356 CCGGG
NspI 614 ACATGC [GA]CATG^[CT]
682 ACATGC
RsaI 88 GTAC GT^AC
690 GTAC
SacI 661 GAGCTC GAGCT^C
804 GAGCTC
StuI 764 AGGCCT AGG^CCT
909 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrFI BssKI BstNI Cac8I CfoI
Cfr10I Cfr13I DpnII EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI StyI TseI