(Circular) (Six-base) MAPSORT of: pFN21AB9443.seq Check: 2998 from: 1 to: 5626 pFN21AB9443 5626 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3436 278 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 1937 Size: 1032 4594 Acc65I G'GTAC_C Cuts at: 2947 2947 Size: 5626 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2282 2522 2891 3689 5073 5341 1632 Size: 515 135 240 369 798 1384 268 1917 Fragments arranged by size: 1917 1384 798 515 369 268 240 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5626 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2847 1184 Size: 1663 3963 AlwNI CAG_nnn'CTG Cuts at: 3369 4941 3369 Size: 1572 4054 ApaLI G'TGCA_C Cuts at: 3683 4839 3683 Size: 1156 4470 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2347 2951 1870 Size: 73 404 604 4545 Fragments arranged by size: 4545 604 404 73 AvrII C'CTAG_G Cuts at: 2911 2911 Size: 5626 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2947 4402 618 Size: 325 217 627 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 325 217 BanII G_rGCy'C Cuts at: 1391 2369 2657 2670 2813 2945 1391 Size: 978 288 13 143 132 4072 Fragments arranged by size: 4072 978 288 143 132 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2602 2851 928 Size: 971 703 249 3703 Fragments arranged by size: 3703 971 703 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2180 4237 136 Size: 107 122 71 631 605 508 2057 1525 Fragments arranged by size: 2057 1525 631 605 508 122 107 71 BglII A'GATC_T Cuts at: 2724 5621 2724 Size: 2897 2729 BlpI GC'TnA_GC Cuts at: 3043 3043 Size: 5626 Bme1580I G_kGCm'C Cuts at: 1790 2012 3687 4843 1790 Size: 222 1675 1156 2573 Fragments arranged by size: 2573 1675 1156 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5626 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3357 4315 442 Size: 416 53 83 399 24 1940 958 1753 Fragments arranged by size: 1940 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 4286 1239 Size: 1415 1632 2579 Fragments arranged by size: 2579 1632 1415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 3751 4616 4914 2723 Size: 1028 865 298 3435 Fragments arranged by size: 3435 1028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4289 882 Size: 617 2790 2219 Fragments arranged by size: 2790 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3417 493 Size: 711 2213 2702 Fragments arranged by size: 2702 2213 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2417 2441 3433 3815 Size: 53 83 186 936 884 24 992 382 Cuts at: 3815 4450 275 Size: 635 1451 Fragments arranged by size: 1451 992 936 884 635 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 2037 4059 4457 4731 4878 5289 2037 Size: 2022 398 274 147 411 2374 Fragments arranged by size: 2374 2022 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 1178 Size: 30 165 30 1308 30 4063 Fragments arranged by size: 4063 1308 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 2362 2632 2897 1186 Size: 1173 3 270 265 3915 Fragments arranged by size: 3915 1173 270 265 3 BseYI C'CCAG_C Cuts at: 2030 2301 2316 4829 5325 2030 Size: 271 15 2513 496 2331 Fragments arranged by size: 2513 2331 496 271 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 2310 1163 Size: 573 492 82 4479 Fragments arranged by size: 4479 573 492 82 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2091 3076 3837 4865 1540 Size: 275 186 90 985 761 1028 2301 Fragments arranged by size: 2301 1028 985 761 275 186 90 BsiHKAI G_wGCw'C Cuts at: 2532 2670 2813 3687 3772 4843 2532 Size: 138 143 874 85 1071 3315 Fragments arranged by size: 3315 1071 874 143 138 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2369 2532 2657 2670 2813 2945 Size: 399 222 357 163 125 13 143 132 Cuts at: 2945 3687 3772 4843 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 222 163 143 132 125 85 13 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 2805 3515 5245 2805 Size: 710 1730 3186 Fragments arranged by size: 3186 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2553 2737 2883 3513 1394 Size: 429 730 184 146 630 3507 Fragments arranged by size: 3507 730 630 429 184 146 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 2487 2596 4270 4457 1700 Size: 110 501 176 109 1674 187 2869 Fragments arranged by size: 2869 1674 501 187 176 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5626 BssSI C'ACGA_G Cuts at: 2663 3686 4698 2663 Size: 1023 1012 3591 Fragments arranged by size: 3591 1023 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 4430 4430 Size: 5626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2724 2956 3323 3708 3725 5177 5263 5275 Size: 752 232 367 385 17 1452 86 12 Cuts at: 5275 5621 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 752 385 367 346 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2517 513 Size: 552 939 69 444 3622 Fragments arranged by size: 3622 939 552 444 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4887 Fragments arranged by size: 4887 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3936 3956 3142 Size: 794 20 4812 Fragments arranged by size: 4812 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2035 2070 529 Size: 35 1471 35 4085 Fragments arranged by size: 4085 1471 35 35 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2088 2172 Size: 54 1425 50 275 18 237 21 84 Cuts at: 2172 2181 2334 2598 2884 3073 3962 5591 8 Size: 9 153 264 286 189 889 1629 43 Fragments arranged by size: 1629 1425 889 286 275 264 237 189 153 84 54 50 43 21 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2088 3073 1537 Size: 275 276 985 4090 Fragments arranged by size: 4090 985 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 3556 1353 Size: 803 1400 3423 Fragments arranged by size: 3423 1400 803 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2668 2811 2668 Size: 143 5483 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2282 2522 2654 2891 3689 4286 Size: 393 515 135 240 132 237 798 597 Cuts at: 4286 5073 5341 1239 Size: 787 268 1524 Fragments arranged by size: 1524 798 787 597 515 393 268 240 237 135 132 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 5626 FspI TGC'GCA Cuts at: 2540 4132 2540 Size: 1592 4034 HaeII r_GCGC'y Cuts at: 1678 2134 4773 1678 Size: 456 2639 2531 Fragments arranged by size: 2639 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 4267 4299 4341 Size: 32 135 32 1052 32 1611 32 42 Cuts at: 4341 4373 1373 Size: 32 2626 Fragments arranged by size: 2626 1611 1052 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2951 2951 Size: 5626 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2889 4740 4924 1305 Size: 1584 1851 184 2007 Fragments arranged by size: 2007 1851 1584 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3804 Fragments arranged by size: 3804 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 3584 3943 4102 518 Size: 648 981 1437 359 159 2042 Fragments arranged by size: 2042 1437 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3042 3719 4867 5112 1606 Size: 1436 677 1148 245 2120 Fragments arranged by size: 2120 1436 1148 677 245 NaeI GCC'GGC Cuts at: 2598 2598 Size: 5626 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4135 Fragments arranged by size: 4135 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NgoMIV G'CCGG_C Cuts at: 2596 2596 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 3073 3073 Size: 5626 NspI r_CATG'y Cuts at: 1132 1229 2623 2691 2984 4529 1132 Size: 97 1394 68 293 1545 2229 Fragments arranged by size: 2229 1545 1394 293 97 68 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5626 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PstI C_TGCA'G Cuts at: 830 2568 2630 2826 2978 830 Size: 1738 62 196 152 3478 Fragments arranged by size: 3478 1738 196 152 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SacI G_AGCT'C Cuts at: 2670 2813 2670 Size: 143 5483 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2564 2626 2822 2974 4109 4790 4981 Size: 219 1519 62 196 152 1135 681 191 Cuts at: 4981 826 Size: 1471 Fragments arranged by size: 1519 1471 1135 681 219 196 191 152 62 SfiI GGCCn_nnn'nGGCC Cuts at: 2180 2180 Size: 5626 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 2487 1700 Size: 787 4839 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2738 3730 4631 4893 820 Size: 197 926 795 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 795 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 3550 5 Size: 47 3498 2081 Fragments arranged by size: 3498 2081 47 StuI AGG'CCT Cuts at: 1517 2771 2916 1517 Size: 1254 145 4227 Fragments arranged by size: 4227 1254 145 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2156 2911 3065 513 Size: 552 939 108 44 755 154 3074 Fragments arranged by size: 3074 939 755 552 154 108 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2459 3653 3670 3823 4008 4453 325 Size: 1255 879 1194 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 879 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3872 5584 96 Size: 274 80 33 51 1443 1895 1712 138 Fragments arranged by size: 1895 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 3755 3755 Size: 5626 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3434 276 Size: 53 83 186 936 1900 2468 Fragments arranged by size: 2468 1900 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI