Restriction map for pFN21AB9431
 (Linear) (Six-base) MAPSORT of: KIBB9431.seq  Check: 3470  from: 1  to: 1572



KIBB9431.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     121     352    1572 
   Size:        121     231    1220

  Fragments arranged by size: 

                1220     231     121
 
AccI GT'mk_AC

Cuts at:      0    1259    1572 
   Size:       1259     313
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     995    1159    1559    1572 
   Size:        439     556     164     400      13

  Fragments arranged by size: 

                 556     439     400     164      13
 
AgeI A'CCGG_T

Cuts at:      0     839    1572 
   Size:        839     733
 
AleI CACnn'nnGTG

Cuts at:      0    1426    1572 
   Size:       1426     146
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     623    1572 
   Size:        623     949
 
AlwNI CAG_nnn'CTG

Cuts at:      0     588     636    1484    1572 
   Size:        588      48     848      88

  Fragments arranged by size: 

                 848     588      88      48
 
ApaI G_GGCC'C

Cuts at:      0    1411    1572 
   Size:       1411     161
 
AvaI C'yCGr_G

Cuts at:      0      59    1303    1572 
   Size:         59    1244     269

  Fragments arranged by size: 

                1244     269      59
 
BanI G'GyrC_C

Cuts at:      0    1327    1572 
   Size:       1327     245
 
BanII G_rGCy'C

Cuts at:      0     899    1241    1312    1339    1411    1478    1571    1572 
   Size:        899     342      71      27      72      67      93       1

  Fragments arranged by size: 

                 899     342      93      72      71      67      27       1
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     515    1052    1559    1572 
   Size:        515     537     507      13

  Fragments arranged by size: 

                 537     515     507      13
 
BbvCI CC'TCA_GC

Cuts at:      0    1040    1572 
   Size:       1040     532
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1098    1132    1572 
   Size:       1098      34     440

  Fragments arranged by size: 

                1098     440      34
 
BclI T'GATC_A

Cuts at:      0     144    1572 
   Size:        144    1428
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1102    1572 
   Size:       1102     470
 
Bme1580I G_kGCm'C

Cuts at:      0     923    1411    1572 
   Size:        923     488     161

  Fragments arranged by size: 

                 923     488     161
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1472    1572 
   Size:       1472     100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    1572 
   Size:       1189     383
 
Bpu10I CC'TnA_GC

Cuts at:      0     758    1040    1572 
   Size:        758     282     532

  Fragments arranged by size: 

                 758     532     282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     690    1444    1572 
   Size:        690     754     128

  Fragments arranged by size: 

                 754     690     128
 
BsaHI Gr'CG_yC

Cuts at:      0      65     326     791    1510    1572 
   Size:         65     261     465     719      62

  Fragments arranged by size: 

                 719     465     261      65      62
 
BsaWI w'CCGG_w

Cuts at:      0     839    1572 
   Size:        839     733
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     358     388    1572 
   Size:        358      30    1184

  Fragments arranged by size: 

                1184     358      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     472     757     919     952     955     958     961     964 
   Size:        472     285     162      33       3       3       3       3

Cuts at:    964     967     970     973     997    1000    1003    1006    1135 
   Size:          3       3       3      24       3       3       3     129

Cuts at:   1135    1165    1572 
   Size:         30     407

  Fragments arranged by size: 

                 472     407     285     162     129      33      30      24
                   3       3       3       3       3       3       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      35     490    1572 
   Size:         35     455    1082

  Fragments arranged by size: 

                1082     455      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213    1027    1572 
   Size:        213     814     545

  Fragments arranged by size: 

                 814     545     213
 
BsiEI CG_ry'CG

Cuts at:      0     810    1135    1572 
   Size:        810     325     437

  Fragments arranged by size: 

                 810     437     325
 
BsiHKAI G_wGCw'C

Cuts at:      0    1241    1571    1572 
   Size:       1241     330       1

  Fragments arranged by size: 

                1241     330       1
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      72    1572 
   Size:         72    1500
 
Bsp1286I G_dGCh'C

Cuts at:      0     899     923    1241    1312    1339    1411    1478    1571 
   Size:        899      24     318      71      27      72      67      93

Cuts at:   1571    1572 
   Size:          1

  Fragments arranged by size: 

                 899     318      93      72      71      67      27      24
                   1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     121     352    1572 
   Size:        121     231    1220

  Fragments arranged by size: 

                1220     231     121
 
BsrBI CCG'CTC

Cuts at:      0    1131    1418    1572 
   Size:       1131     287     154

  Fragments arranged by size: 

                1131     287     154
 
BsrFI r'CCGG_y

Cuts at:      0     839    1026    1572 
   Size:        839     187     546

  Fragments arranged by size: 

                 839     546     187
 
BsrGI T'GTAC_A

Cuts at:      0    1220    1572 
   Size:       1220     352
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     877    1397    1572 
   Size:        877     520     175

  Fragments arranged by size: 

                 877     520     175
 
BstEII G'GTnAC_C

Cuts at:      0     396    1458    1572 
   Size:        396    1062     114

  Fragments arranged by size: 

                1062     396     114
 
BstYI r'GATC_y

Cuts at:      0     425    1572 
   Size:        425    1147
 
BtgI C'CryG_G

Cuts at:      0      41    1454    1572 
   Size:         41    1413     118

  Fragments arranged by size: 

                1413     118      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     314    1572 
   Size:        314    1258
 
BtsI GCAGTG_nn'

Cuts at:      0     225     850    1572 
   Size:        225     625     722

  Fragments arranged by size: 

                 722     625     225
 
ClaI AT'CG_AT

Cuts at:      0    1088    1572 
   Size:       1088     484
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1305    1340    1572 
   Size:       1305      35     232

  Fragments arranged by size: 

                1305     232      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     682    1572 
   Size:        682     890
 
EaeI y'GGCC_r

Cuts at:      0     128     389    1028    1572 
   Size:        128     261     639     544

  Fragments arranged by size: 

                 639     544     261     128
 
EarI CTCTTCn'nnn_

Cuts at:      0     590     962     974    1572 
   Size:        590     372      12     598

  Fragments arranged by size: 

                 598     590     372      12
 
EcoICRI GAG'CTC

Cuts at:      0    1239    1569    1572 
   Size:       1239     330       3

  Fragments arranged by size: 

                1239     330       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439     995    1159    1189    1559    1572 
   Size:        439     556     164      30     370      13

  Fragments arranged by size: 

                 556     439     370     164      30      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     433    1572 
   Size:        433    1139
 
EcoO109I rG'GnC_Cy

Cuts at:      0      56     762    1407    1408    1572 
   Size:         56     706     645       1     164

  Fragments arranged by size: 

                 706     645     164      56       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     781     813    1572 
   Size:        358      32     391      32     759

  Fragments arranged by size: 

                 759     391     358      32      32
 
HincII GTy'rAC

Cuts at:      0     153     276    1572 
   Size:        153     123    1296

  Fragments arranged by size: 

                1296     153     123
 
HindIII A'AGCT_T

Cuts at:      0    1279    1572 
   Size:       1279     293
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290    1572 
   Size:        290    1282
 
MslI CAynn'nnrTG

Cuts at:      0    1426    1572 
   Size:       1426     146
 
MspA1I CmG'CkG

Cuts at:      0      12     323     603    1572 
   Size:         12     311     280     969

  Fragments arranged by size: 

                 969     311     280      12
 
NaeI GCC'GGC

Cuts at:      0    1028    1572 
   Size:       1028     544
 
NcoI C'CATG_G

Cuts at:      0    1454    1572 
   Size:       1454     118
 
NgoMIV G'CCGG_C

Cuts at:      0    1026    1572 
   Size:       1026     546
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      35    1572 
   Size:         35    1537
 
PspOMI G'GGCC_C

Cuts at:      0    1407    1572 
   Size:       1407     165
 
PstI C_TGCA'G

Cuts at:      0     383     704    1572 
   Size:        383     321     868

  Fragments arranged by size: 

                 868     383     321
 
PvuII CAG'CTG

Cuts at:      0     603    1572 
   Size:        603     969
 
RsrII CG'GwC_CG

Cuts at:      0     842    1572 
   Size:        842     730
 
SacI G_AGCT'C

Cuts at:      0    1241    1571    1572 
   Size:       1241     330       1

  Fragments arranged by size: 

                1241     330       1
 
SapI GCTCTTCn'nnn_

Cuts at:      0     590    1572 
   Size:        590     982
 
SexAI A'CCwGG_T

Cuts at:      0     512    1572 
   Size:        512    1060
 
SfcI C'TryA_G

Cuts at:      0     379     484     700    1572 
   Size:        379     105     216     872

  Fragments arranged by size: 

                 872     379     216     105
 
SmaI CCC'GGG

Cuts at:      0      61    1572 
   Size:         61    1511
 
StuI AGG'CCT

Cuts at:      0    1081    1572 
   Size:       1081     491
 
StyI C'CwwG_G

Cuts at:      0     614     899    1323    1454    1572 
   Size:        614     285     424     131     118

  Fragments arranged by size: 

                 614     424     285     131     118
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1560    1572 
   Size:       1560      12
 
TatI w'GTAC_w

Cuts at:      0     314    1220    1572 
   Size:        314     906     352

  Fragments arranged by size: 

                 906     352     314
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     849     881    1572 
   Size:        849      32     691

  Fragments arranged by size: 

                 849     691      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     811    1572 
   Size:        811     761
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1164    1572 
   Size:       1164     408
 
XmaI C'CCGG_G

Cuts at:      0      59    1572 
   Size:         59    1513
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AgeI     AleI     AlfI    AlwNI     ApaI
     AvaI     BanI    BanII     BbsI    BbvCI     BcgI     BclI     BglI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI   BstAPI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I
     NaeI     NcoI   NgoMIV    PflMI   PspOMI     PstI    PvuII    RsrII
     SacI     SapI    SexAI     SfcI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsiWI     BsmI    BspEI    BspHI    BsrDI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I     DraI   DraIII
     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    SacII     SalI    SanDI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI     ZraI
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