Restriction map for
pFN21AB9426
(Linear) (Six-base) MAPSORT of: KIBB9426.seq Check: 8061 from: 1 to: 2049
KIBB9426.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 1604 1715 2049
Size: 1604 111 334
Fragments arranged by size:
1604 334 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 805 1177 2049
Size: 443 362 372 872
Fragments arranged by size:
872 443 372 362
AlwNI CAG_nnn'CTG
Cuts at: 0 120 321 2049
Size: 120 201 1728
Fragments arranged by size:
1728 201 120
ApaI G_GGCC'C
Cuts at: 0 1860 2049
Size: 1860 189
ApaLI G'TGCA_C
Cuts at: 0 615 2049
Size: 615 1434
AvaI C'yCGr_G
Cuts at: 0 244 370 1910 2049
Size: 244 126 1540 139
Fragments arranged by size:
1540 244 139 126
BamHI G'GATC_C
Cuts at: 0 1214 1748 2049
Size: 1214 534 301
Fragments arranged by size:
1214 534 301
BanI G'GyrC_C
Cuts at: 0 10 337 2049
Size: 10 327 1712
Fragments arranged by size:
1712 327 10
BanII G_rGCy'C
Cuts at: 0 1368 1860 2049
Size: 1368 492 189
Fragments arranged by size:
1368 492 189
BbeI G_GCGC'C
Cuts at: 0 341 2049
Size: 341 1708
BbsI GAAGACnn'nnnn_
Cuts at: 0 190 249 2049
Size: 190 59 1800
Fragments arranged by size:
1800 190 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 606 640 2049
Size: 606 34 1409
Fragments arranged by size:
1409 606 34
BglII A'GATC_T
Cuts at: 0 1434 2049
Size: 1434 615
Bme1580I G_kGCm'C
Cuts at: 0 619 1860 2049
Size: 619 1241 189
Fragments arranged by size:
1241 619 189
BmgBI CAC'GTC
Cuts at: 0 1167 2049
Size: 1167 882
BmrI ACTGGGnnnn_n'
Cuts at: 0 1863 2049
Size: 1863 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1550 1561 2018 2049
Size: 418 1132 11 457 31
Fragments arranged by size:
1132 457 418 31 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1845 2049
Size: 1845 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 934 1127 2049
Size: 434 500 193 922
Fragments arranged by size:
922 500 434 193
BsaAI yAC'GTr
Cuts at: 0 200 530 2049
Size: 200 330 1519
Fragments arranged by size:
1519 330 200
BsaBI GATnn'nnATC
Cuts at: 0 727 2049
Size: 727 1322
BsaHI Gr'CG_yC
Cuts at: 0 268 338 2049
Size: 268 70 1711
Fragments arranged by size:
1711 268 70
BsaWI w'CCGG_w
Cuts at: 0 443 2049
Size: 443 1606
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 2049
Size: 223 1826
BseYI C'CCAG_C
Cuts at: 0 1859 2049
Size: 1859 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 2049
Size: 285 1764
BsiEI CG_ry'CG
Cuts at: 0 335 1281 2049
Size: 335 946 768
Fragments arranged by size:
946 768 335
BsiHKAI G_wGCw'C
Cuts at: 0 619 1368 1817 2049
Size: 619 749 449 232
Fragments arranged by size:
749 619 449 232
BsmI GAATG_Cn'
Cuts at: 0 1198 2049
Size: 1198 851
BsmBI CGTCTCn'nnnn_
Cuts at: 0 259 2049
Size: 259 1790
Bsp1286I G_dGCh'C
Cuts at: 0 619 1368 1817 1860 2049
Size: 619 749 449 43 189
Fragments arranged by size:
749 619 449 189 43
BspHI T'CATG_A
Cuts at: 0 1763 2049
Size: 1763 286
BsrDI GCAATG_nn'
Cuts at: 0 1524 1622 2049
Size: 1524 98 427
Fragments arranged by size:
1524 427 98
BsrFI r'CCGG_y
Cuts at: 0 334 2049
Size: 334 1715
BssSI C'ACGA_G
Cuts at: 0 262 1361 1465 2049
Size: 262 1099 104 584
Fragments arranged by size:
1099 584 262 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 508 915 1788 2049
Size: 508 407 873 261
Fragments arranged by size:
873 508 407 261
BstYI r'GATC_y
Cuts at: 0 366 446 1214 1434 1571 1748 2049
Size: 366 80 768 220 137 177 301
Fragments arranged by size:
768 366 301 220 177 137 80
BtgI C'CryG_G
Cuts at: 0 1529 2049
Size: 1529 520
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1855 2049
Size: 1855 194
BtsI GCAGTG_nn'
Cuts at: 0 62 558 790 2049
Size: 62 496 232 1259
Fragments arranged by size:
1259 496 232 62
DraIII CAC_nnn'GTG
Cuts at: 0 291 2049
Size: 291 1758
EaeI y'GGCC_r
Cuts at: 0 237 251 293 524 1532 2049
Size: 237 14 42 231 1008 517
Fragments arranged by size:
1008 517 237 231 42 14
EarI CTCTTCn'nnn_
Cuts at: 0 103 1946 2049
Size: 103 1843 103
Fragments arranged by size:
1843 103 103
EcoICRI GAG'CTC
Cuts at: 0 1366 2049
Size: 1366 683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 443 805 1177 1550 1561 2018 2049
Size: 418 25 362 372 373 11 457 31
Fragments arranged by size:
457 418 373 372 362 31 25 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1687 1801 2049
Size: 1687 114 248
Fragments arranged by size:
1687 248 114
FspI TGC'GCA
Cuts at: 0 328 2049
Size: 328 1721
HaeII r_GCGC'y
Cuts at: 0 341 474 2049
Size: 341 133 1575
Fragments arranged by size:
1575 341 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 431 463 1037 1069 1206 1238 1263 1295
Size: 431 32 574 32 137 32 25 32
Cuts at: 1295 1886 1904 1918 1936 2049
Size: 591 18 14 18 113
Fragments arranged by size:
591 574 431 137 113 32 32 32
32 25 18 18 14
KasI G'GCGC_C
Cuts at: 0 337 2049
Size: 337 1712
MfeI C'AATT_G
Cuts at: 0 1970 2049
Size: 1970 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 976 1064 1439 2049
Size: 957 19 88 375 610
Fragments arranged by size:
957 610 375 88 19
MscI TGG'CCA
Cuts at: 0 295 526 2049
Size: 295 231 1523
Fragments arranged by size:
1523 295 231
MslI CAynn'nnrTG
Cuts at: 0 1075 1736 2049
Size: 1075 661 313
Fragments arranged by size:
1075 661 313
MspA1I CmG'CkG
Cuts at: 0 77 117 1863 2049
Size: 77 40 1746 186
Fragments arranged by size:
1746 186 77 40
NarI GG'CG_CC
Cuts at: 0 338 2049
Size: 338 1711
PasI CC'CwG_GG
Cuts at: 0 273 2049
Size: 273 1776
PflMI CCAn_nnn'nTGG
Cuts at: 0 77 120 291 2049
Size: 77 43 171 1758
Fragments arranged by size:
1758 171 77 43
PfoI T'CCnGG_A
Cuts at: 0 314 1577 2049
Size: 314 1263 472
Fragments arranged by size:
1263 472 314
PspOMI G'GGCC_C
Cuts at: 0 1856 2049
Size: 1856 193
PspXI vC'TCGA_Gb
Cuts at: 0 244 2049
Size: 244 1805
PstI C_TGCA'G
Cuts at: 0 68 311 565 1178 1383 1602 2049
Size: 68 243 254 613 205 219 447
Fragments arranged by size:
613 447 254 243 219 205 68
PvuI CG_AT'CG
Cuts at: 0 1281 2049
Size: 1281 768
PvuII CAG'CTG
Cuts at: 0 117 1863 2049
Size: 117 1746 186
Fragments arranged by size:
1746 186 117
SacI G_AGCT'C
Cuts at: 0 1368 2049
Size: 1368 681
SapI GCTCTTCn'nnn_
Cuts at: 0 103 1946 2049
Size: 103 1843 103
Fragments arranged by size:
1843 103 103
SexAI A'CCwGG_T
Cuts at: 0 149 2049
Size: 149 1900
SfcI C'TryA_G
Cuts at: 0 64 307 561 1174 1379 1428 1598 2049
Size: 64 243 254 613 205 49 170 451
Fragments arranged by size:
613 451 254 243 205 170 64 49
SfoI GGC'GCC
Cuts at: 0 339 2049
Size: 339 1710
SmlI C'TyrA_G
Cuts at: 0 244 370 1824 2049
Size: 244 126 1454 225
Fragments arranged by size:
1454 244 225 126
SspI AAT'ATT
Cuts at: 0 681 945 2049
Size: 681 264 1104
Fragments arranged by size:
1104 681 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 351 1219 2049
Size: 351 868 830
Fragments arranged by size:
868 830 351
TatI w'GTAC_w
Cuts at: 0 664 2049
Size: 664 1385
Tth111I GACn'n_nGTC
Cuts at: 0 1450 2049
Size: 1450 599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 505 1785 2049
Size: 505 1280 264
Fragments arranged by size:
1280 505 264
XhoI C'TCGA_G
Cuts at: 0 244 370 2049
Size: 244 126 1679
Fragments arranged by size:
1679 244 126
XmnI GAAnn'nnTTC
Cuts at: 0 945 2019 2049
Size: 945 1074 30
Fragments arranged by size:
1074 945 30
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BbeI BbsI BcgI BglII Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI FspI HaeII
Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI
PasI PflMI PfoI PspOMI PspXI PstI PvuI PvuII
SacI SapI SexAI SfcI SfoI SmlI SspI TaqII
TatI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BfrBI BglI BlpI
BmtI BplI Bpu10I BsaXI BsiWI BspEI BspMI BsrBI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoO109I EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI RsrII SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TstI XbaI XmaI ZraI