(Circular) (Six-base) MAPSORT of: pFN21AB9419.seq Check: 2192 from: 1 to: 6334 pFN21AB9419 6334 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 3045 3348 4397 5781 6049 1632 Size: 492 921 303 1049 1384 268 1917 Fragments arranged by size: 1917 1384 1049 921 492 303 268 AflII C'TTAA_G Cuts at: 820 1017 3083 820 Size: 197 2066 4071 Fragments arranged by size: 4071 2066 197 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 2793 5063 2793 Size: 2270 4064 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2075 3326 4077 5649 2075 Size: 1251 751 1572 2760 Fragments arranged by size: 2760 1572 1251 751 ApaLI G'TGCA_C Cuts at: 4391 5547 4391 Size: 1156 5178 ApoI r'AATT_y Cuts at: 1493 3050 3302 3381 3628 3643 3869 4021 6130 Size: 1557 252 79 247 15 226 152 2109 Cuts at: 6130 1493 Size: 1697 Fragments arranged by size: 2109 1697 1557 252 247 226 152 79 15 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2431 2823 3659 1870 Size: 73 488 392 836 4545 Fragments arranged by size: 4545 836 488 392 73 BamHI G'GATC_C Cuts at: 2195 3664 4031 2195 Size: 1469 367 4498 Fragments arranged by size: 4498 1469 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2245 3655 5110 618 Size: 325 217 627 458 1410 1455 1842 Fragments arranged by size: 1842 1455 1410 627 458 325 217 BanII G_rGCy'C Cuts at: 1391 3653 1391 Size: 2262 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2334 3276 3591 928 Size: 971 435 942 315 3671 Fragments arranged by size: 3671 971 942 435 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2660 3461 4226 5436 2660 Size: 801 765 1210 3558 Fragments arranged by size: 3558 1210 801 765 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3489 4945 136 Size: 107 122 71 631 605 1817 1456 1525 Fragments arranged by size: 1817 1525 1456 631 605 122 107 71 BglII A'GATC_T Cuts at: 3190 6329 3190 Size: 3139 3195 BlpI GC'TnA_GC Cuts at: 3751 3751 Size: 6334 Bme1580I G_kGCm'C Cuts at: 1790 3485 4395 5551 1790 Size: 1695 910 1156 2573 Fragments arranged by size: 2573 1695 1156 910 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6334 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 5023 442 Size: 416 53 83 399 24 2648 958 1753 Fragments arranged by size: 2648 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 4994 1239 Size: 813 2942 2579 Fragments arranged by size: 2942 2579 813 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2775 4459 5324 5622 2775 Size: 1684 865 298 3487 Fragments arranged by size: 3487 1684 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4125 493 Size: 711 2921 2702 Fragments arranged by size: 2921 2702 711 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4523 5158 275 Size: 53 83 186 936 2608 382 635 1451 Fragments arranged by size: 2608 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6109 Fragments arranged by size: 6109 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6334 BseYI C'CCAG_C Cuts at: 5537 6033 5537 Size: 496 5838 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3318 1163 Size: 573 1582 4179 Fragments arranged by size: 4179 1582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2689 4395 4480 5551 2689 Size: 1706 85 1071 3472 Fragments arranged by size: 3472 1706 1071 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2689 3485 3653 4395 4480 5551 1391 Size: 399 899 796 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 796 742 399 168 85 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3689 844 Size: 2845 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4221 1394 Size: 429 2398 3507 Fragments arranged by size: 3507 2398 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4978 5165 1700 Size: 110 3168 187 2869 Fragments arranged by size: 3168 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 2807 794 Size: 2013 4321 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 3315 3315 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2195 3190 3664 4031 4416 4433 5885 5971 Size: 223 995 474 367 385 17 1452 86 Cuts at: 5971 5983 6329 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 995 474 385 367 346 223 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2200 3486 2200 Size: 1286 5048 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5595 Fragments arranged by size: 5595 683 56 BtsI GCAGTG_nn' Cuts at: 3594 3850 4644 4664 3594 Size: 256 794 20 5264 Fragments arranged by size: 5264 794 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5341 809 Size: 507 4025 1802 Fragments arranged by size: 4025 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299 Size: 54 1425 50 275 18 1951 889 1629 Cuts at: 6299 8 Size: 43 Fragments arranged by size: 1951 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4264 1353 Size: 2911 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2124 3045 3348 4397 4994 5781 Size: 393 420 72 921 303 1049 597 787 Cuts at: 5781 6049 1239 Size: 268 1524 Fragments arranged by size: 1524 1049 921 787 597 420 393 303 268 72 EcoNI CCTnn'n_nnAGG Cuts at: 2217 2217 Size: 6334 EcoO109I rG'GnC_Cy Cuts at: 3538 3538 Size: 6334 EcoRI G'AATT_C Cuts at: 3381 3643 4021 3381 Size: 262 378 5694 Fragments arranged by size: 5694 378 262 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2972 2972 Size: 6334 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2450 5481 1678 Size: 772 3031 2531 Fragments arranged by size: 3031 2531 772 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2658 2690 2779 2811 3356 Size: 32 135 32 1086 32 89 32 545 Cuts at: 3356 3388 4975 5007 5049 5081 1373 Size: 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 1086 545 135 89 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2757 3678 3924 669 Size: 988 281 819 921 246 3079 Fragments arranged by size: 3079 988 921 819 281 246 HindIII A'AGCT_T Cuts at: 748 2949 3067 3110 748 Size: 2201 118 43 3972 Fragments arranged by size: 3972 2201 118 43 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2795 3509 5448 5632 1305 Size: 1490 714 1939 184 2007 Fragments arranged by size: 2007 1939 1490 714 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4512 Fragments arranged by size: 4512 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2736 4292 4651 4810 518 Size: 648 951 619 1556 359 159 2042 Fragments arranged by size: 2042 1556 951 648 619 359 159 MspA1I CmG'CkG Cuts at: 1606 3121 3750 4427 5575 5820 1606 Size: 1515 629 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 677 629 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4843 Fragments arranged by size: 4843 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2735 3692 5237 1132 Size: 97 1506 957 1545 2229 Fragments arranged by size: 2229 1545 1506 957 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 2527 2527 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3538 3538 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PstI C_TGCA'G Cuts at: 830 2455 2706 3686 830 Size: 1625 251 980 3478 Fragments arranged by size: 3478 1625 980 251 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 3121 3121 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 3447 4582 6294 3447 Size: 1135 1712 3487 Fragments arranged by size: 3487 1712 1135 SexAI A'CCwGG_T Cuts at: 2929 2929 Size: 6334 SfcI C'TryA_G Cuts at: 826 1045 2115 2322 2409 2451 2702 3600 3682 Size: 219 1070 207 87 42 251 898 82 Cuts at: 3682 4817 5498 5689 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1070 898 681 251 219 207 191 87 82 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3661 1872 Size: 1789 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2790 2823 3083 4438 5339 5601 Size: 197 926 847 33 260 1355 901 262 Cuts at: 5601 820 Size: 1553 Fragments arranged by size: 1553 1355 926 901 847 262 260 197 33 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 3397 152 Size: 3245 3089 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SspI AAT'ATT Cuts at: 5 52 3208 4258 5 Size: 47 3156 1050 2081 Fragments arranged by size: 3156 2081 1050 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2004 3773 513 Size: 552 939 1769 3074 Fragments arranged by size: 3074 1769 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4361 4378 4531 4716 5161 325 Size: 1255 2781 17 153 185 445 1498 Fragments arranged by size: 2781 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2081 3445 4580 Size: 274 80 33 51 1443 104 1364 1135 Cuts at: 4580 6292 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1364 1135 274 138 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6294 6326 6294 Size: 32 6302 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XhoI C'TCGA_G Cuts at: 1943 2823 1943 Size: 880 5454 XmaI C'CCGG_G Cuts at: 1870 3659 1870 Size: 1789 4545 XmnI GAAnn'nnTTC Cuts at: 2355 3567 4463 2355 Size: 1212 896 4226 Fragments arranged by size: 4226 1212 896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI