Restriction map for pFN21AB9419
 (Circular) (Six-base) MAPSORT of: pFN21AB9419.seq  Check: 2192  from: 1  to: 6334



pFN21AB9419  6334 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4144     278 
   Size:         53      83     186     936    2608    2468

  Fragments arranged by size: 

                2608    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3677    1937 
   Size:       1740    4594
 
Acc65I G'GTAC_C

Cuts at:   3655    3655 
   Size:       6334
 
AclI AA'CG_TT

Cuts at:   1563    4461    4834    1563 
   Size:       2898     373    3063

  Fragments arranged by size: 

                3063    2898     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2124    3045    3348    4397    5781    6049    1632 
   Size:        492     921     303    1049    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1049     921     492     303     268
 
AflII C'TTAA_G

Cuts at:    820    1017    3083     820 
   Size:        197    2066    4071

  Fragments arranged by size: 

                4071    2066     197
 
AflIII A'CryG_T

Cuts at:   1203    5233    6210    1203 
   Size:       4030     977    1327

  Fragments arranged by size: 

                4030    1327     977
 
AgeI A'CCGG_T

Cuts at:   5165    5165 
   Size:       6334
 
AhdI GACnn_n'nnGTC

Cuts at:   2793    5063    2793 
   Size:       2270    4064
 
AleI CACnn'nnGTG

Cuts at:   1166    2117    1166 
   Size:        951    5383
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6334
 
AlwNI CAG_nnn'CTG

Cuts at:   2075    3326    4077    5649    2075 
   Size:       1251     751    1572    2760

  Fragments arranged by size: 

                2760    1572    1251     751
 
ApaLI G'TGCA_C

Cuts at:   4391    5547    4391 
   Size:       1156    5178
 
ApoI r'AATT_y

Cuts at:   1493    3050    3302    3381    3628    3643    3869    4021    6130 
   Size:       1557     252      79     247      15     226     152    2109

Cuts at:   6130    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697    1557     252     247     226     152      79
                  15
 
AseI AT'TA_AT

Cuts at:    160    1032    4888     160 
   Size:        872    3856    1606

  Fragments arranged by size: 

                3856    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6334
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2431    2823    3659    1870 
   Size:         73     488     392     836    4545

  Fragments arranged by size: 

                4545     836     488     392      73
 
BamHI G'GATC_C

Cuts at:   2195    3664    4031    2195 
   Size:       1469     367    4498

  Fragments arranged by size: 

                4498    1469     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2245    3655    5110     618 
   Size:        325     217     627     458    1410    1455    1842

  Fragments arranged by size: 

                1842    1455    1410     627     458     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3653    1391 
   Size:       2262    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2334    3276    3591     928 
   Size:        971     435     942     315    3671

  Fragments arranged by size: 

                3671     971     942     435     315
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4525    4559    1903 
   Size:         34    2588      34    3678

  Fragments arranged by size: 

                3678    2588      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2660    3461    4226    5436    2660 
   Size:        801     765    1210    3558

  Fragments arranged by size: 

                3558    1210     801     765
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6334
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3489    4945     136 
   Size:        107     122      71     631     605    1817    1456    1525

  Fragments arranged by size: 

                1817    1525    1456     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   3190    6329    3190 
   Size:       3139    3195
 
BlpI GC'TnA_GC

Cuts at:   3751    3751 
   Size:       6334
 
Bme1580I G_kGCm'C

Cuts at:   1790    3485    4395    5551    1790 
   Size:       1695     910    1156    2573

  Fragments arranged by size: 

                2573    1695    1156     910
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6334
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4065    5023     442 
   Size:        416      53      83     399      24    2648     958    1753

  Fragments arranged by size: 

                2648    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2052    4994    1239 
   Size:        813    2942    2579

  Fragments arranged by size: 

                2942    2579     813
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2775    4459    5324    5622    2775 
   Size:       1684     865     298    3487

  Fragments arranged by size: 

                3487    1684     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4997     882 
   Size:        617    3498    2219

  Fragments arranged by size: 

                3498    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4125     493 
   Size:        711    2921    2702

  Fragments arranged by size: 

                2921    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6328    6328 
   Size:       6334
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4141    4523    5158     275 
   Size:         53      83     186     936    2608     382     635    1451

  Fragments arranged by size: 

                2608    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4767    5165    5439    5586    5997    4767 
   Size:        398     274     147     411    5104

  Fragments arranged by size: 

                5104     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6109

  Fragments arranged by size: 

                6109     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6334
 
BseYI C'CCAG_C

Cuts at:   5537    6033    5537 
   Size:        496    5838
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3318    1163 
   Size:        573    1582    4179

  Fragments arranged by size: 

                4179    1582     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3784    4545    5573    1540 
   Size:        275     186    1783     761    1028    2301

  Fragments arranged by size: 

                2301    1783    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2689    4395    4480    5551    2689 
   Size:       1706      85    1071    3472

  Fragments arranged by size: 

                3472    1706    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3845    3938    3845 
   Size:         93    6241
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2689    3485    3653    4395    4480    5551    1391 
   Size:        399     899     796     168     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     899     796     742     399     168      85
            
 
BspEI T'CCGG_A

Cuts at:   5997    5997 
   Size:       6334
 
BspHI T'CATG_A

Cuts at:   4223    5953    4223 
   Size:       1730    4604
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3689     844 
   Size:       2845    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4221    1394 
   Size:        429    2398    3507

  Fragments arranged by size: 

                3507    2398     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4829    5003      65 
   Size:       1183    3581     174    1396

  Fragments arranged by size: 

                3581    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4978    5165    1700 
   Size:        110    3168     187    2869

  Fragments arranged by size: 

                3168    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6334
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6334
 
BssSI C'ACGA_G

Cuts at:   4394    5406    4394 
   Size:       1012    5322
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2807     794 
   Size:       2013    4321
 
BstBI TT'CG_AA

Cuts at:   5138    5138 
   Size:       6334
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6334
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3315    3315 
   Size:       6334
 
BstYI r'GATC_y

Cuts at:   1972    2195    3190    3664    4031    4416    4433    5885    5971 
   Size:        223     995     474     367     385      17    1452      86

Cuts at:   5971    5983    6329    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     995     474     385     367     346     223
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2200    3486    2200 
   Size:       1286    5048
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4843

  Fragments arranged by size: 

                4843     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5595

  Fragments arranged by size: 

                5595     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3594    3850    4644    4664    3594 
   Size:        256     794      20    5264

  Fragments arranged by size: 

                5264     794     256      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6334
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6299
 
DraI TTT'AAA

Cuts at:   3638    3638 
   Size:       6334
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5341     809 
   Size:        507    4025    1802

  Fragments arranged by size: 

                4025    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3781    4670    6299 
   Size:         54    1425      50     275      18    1951     889    1629

Cuts at:   6299       8 
   Size:         43

  Fragments arranged by size: 

                1951    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3781    1537 
   Size:        275    1969    4090

  Fragments arranged by size: 

                4090    1969     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4264    1353 
   Size:       2911    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4921    5295    5441     387 
   Size:       1064     138    3332     374     146    1280

  Fragments arranged by size: 

                3332    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2052    2124    3045    3348    4397    4994    5781 
   Size:        393     420      72     921     303    1049     597     787

Cuts at:   5781    6049    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1049     921     787     597     420     393     303
                 268      72
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2217    2217 
   Size:       6334
 
EcoO109I rG'GnC_Cy

Cuts at:   3538    3538 
   Size:       6334
 
EcoRI G'AATT_C

Cuts at:   3381    3643    4021    3381 
   Size:        262     378    5694

  Fragments arranged by size: 

                5694     378     262
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2972    2972 
   Size:       6334
 
FspI TGC'GCA

Cuts at:   4840    4840 
   Size:       6334
 
HaeII r_GCGC'y

Cuts at:   1678    2450    5481    1678 
   Size:        772    3031    2531

  Fragments arranged by size: 

                3031    2531     772
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2658    2690    2779    2811    3356 
   Size:         32     135      32    1086      32      89      32     545

Cuts at:   3356    3388    4975    5007    5049    5081    1373 
   Size:         32    1587      32      42      32    2626

  Fragments arranged by size: 

                2626    1587    1086     545     135      89      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2757    3678    3924     669 
   Size:        988     281     819     921     246    3079

  Fragments arranged by size: 

                3079     988     921     819     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2949    3067    3110     748 
   Size:       2201     118      43    3972

  Fragments arranged by size: 

                3972    2201     118      43
 
HpaI GTT'AAC

Cuts at:   3924    3924 
   Size:       6334
 
KpnI G_GTAC'C

Cuts at:   3659    3659 
   Size:       6334
 
MfeI C'AATT_G

Cuts at:   3933    3933 
   Size:       6334
 
MluI A'CGCG_T

Cuts at:   6210    6210 
   Size:       6334
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2795    3509    5448    5632    1305 
   Size:       1490     714    1939     184    2007

  Fragments arranged by size: 

                2007    1939    1490     714     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4512

  Fragments arranged by size: 

                4512    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2117    2736    4292    4651    4810     518 
   Size:        648     951     619    1556     359     159    2042

  Fragments arranged by size: 

                2042    1556     951     648     619     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3121    3750    4427    5575    5820    1606 
   Size:       1515     629     677    1148     245    2120

  Fragments arranged by size: 

                2120    1515    1148     677     629     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4843

  Fragments arranged by size: 

                4843     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6334
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6334
 
NotI GC'GGCC_GC

Cuts at:   3781    3781 
   Size:       6334
 
NspI r_CATG'y

Cuts at:   1132    1229    2735    3692    5237    1132 
   Size:         97    1506     957    1545    2229

  Fragments arranged by size: 

                2229    1545    1506     957      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6334
 
PciI A'CATG_T

Cuts at:   5233    5233 
   Size:       6334
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6334
 
PfoI T'CCnGG_A

Cuts at:   2527    2527 
   Size:       6334
 
PmeI GTTT'AAAC

Cuts at:   3638    3638 
   Size:       6334
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4403    4435    5916    5948    4403 
   Size:         32    1481      32    4789

  Fragments arranged by size: 

                4789    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3538    3538 
   Size:       6334
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6334
 
PsiI TTA'TAA

Cuts at:   3904    3904 
   Size:       6334
 
PstI C_TGCA'G

Cuts at:    830    2455    2706    3686     830 
   Size:       1625     251     980    3478

  Fragments arranged by size: 

                3478    1625     980     251
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6334
 
PvuII CAG'CTG

Cuts at:   3121    3121 
   Size:       6334
 
SalI G'TCGA_C

Cuts at:   1936    3676    1936 
   Size:       1740    4594
 
SbfI CC_TGCA'GG

Cuts at:   3686    3686 
   Size:       6334
 
ScaI AGT'ACT

Cuts at:   3447    4582    6294    3447 
   Size:       1135    1712    3487

  Fragments arranged by size: 

                3487    1712    1135
 
SexAI A'CCwGG_T

Cuts at:   2929    2929 
   Size:       6334
 
SfcI C'TryA_G

Cuts at:    826    1045    2115    2322    2409    2451    2702    3600    3682 
   Size:        219    1070     207      87      42     251     898      82

Cuts at:   3682    4817    5498    5689     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1070     898     681     251     219     207
                 191      87      82      42
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6334
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6334
 
SmaI CCC'GGG

Cuts at:   1872    3661    1872 
   Size:       1789    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2790    2823    3083    4438    5339    5601 
   Size:        197     926     847      33     260    1355     901     262

Cuts at:   5601     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1355     926     901     847     262     260     197
                  33
 
SnaBI TAC'GTA

Cuts at:    493    4125     493 
   Size:       3632    2702
 
SpeI A'CTAG_T

Cuts at:    152    3397     152 
   Size:       3245    3089
 
SphI G_CATG'C

Cuts at:   1132    3692    1132 
   Size:       2560    3774
 
SspI AAT'ATT

Cuts at:      5      52    3208    4258       5 
   Size:         47    3156    1050    2081

  Fragments arranged by size: 

                3156    2081    1050      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6334
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3773     513 
   Size:        552     939    1769    3074

  Fragments arranged by size: 

                3074    1769     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4361    4378    4531    4716    5161     325 
   Size:       1255    2781      17     153     185     445    1498

  Fragments arranged by size: 

                2781    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2081    3445    4580 
   Size:        274      80      33      51    1443     104    1364    1135

Cuts at:   4580    6292      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1364    1135     274     138     104      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6294    6326    6294 
   Size:         32    6302
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6334
 
XbaI T'CTAG_A

Cuts at:   3670    3670 
   Size:       6334
 
XhoI C'TCGA_G

Cuts at:   1943    2823    1943 
   Size:        880    5454
 
XmaI C'CCGG_G

Cuts at:   1870    3659    1870 
   Size:       1789    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2355    3567    4463    2355 
   Size:       1212     896    4226

  Fragments arranged by size: 

                4226    1212     896
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4142     276 
   Size:         53      83     186     936    2608    2468

  Fragments arranged by size: 

                2608    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII
  EcoICRI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
     XcmI
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