Restriction map for pFN21AB9418
 (Linear) (Six-base) MAPSORT of: KIBB9418.seq  Check: 4983  from: 1  to: 1857



KIBB9418.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     453    1857 
   Size:        453    1404
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     404     464     914    1566    1646    1742    1857 
   Size:        227     177      60     450     652      80      96     115

  Fragments arranged by size: 

                 652     450     227     177     115      96      80      60
            
 
AfeI AGC'GCT

Cuts at:      0     850    1857 
   Size:        850    1007
 
AflIII A'CryG_T

Cuts at:      0     111    1857 
   Size:        111    1746
 
AleI CACnn'nnGTG

Cuts at:      0     415    1857 
   Size:        415    1442
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1381    1857 
   Size:       1381     476
 
ApaI G_GGCC'C

Cuts at:      0    1501    1857 
   Size:       1501     356
 
ApoI r'AATT_y

Cuts at:      0      37    1044    1233    1857 
   Size:         37    1007     189     624

  Fragments arranged by size: 

                1007     624     189      37
 
AvrII C'CTAG_G

Cuts at:      0    1297    1857 
   Size:       1297     560
 
BamHI G'GATC_C

Cuts at:      0     906    1857 
   Size:        906     951
 
BanII G_rGCy'C

Cuts at:      0     170    1477    1501    1857 
   Size:        170    1307      24     356

  Fragments arranged by size: 

                1307     356     170      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     920    1857 
   Size:        920     937
 
BbvCI CC'TCA_GC

Cuts at:      0     305    1857 
   Size:        305    1552
 
BglII A'GATC_T

Cuts at:      0     882    1857 
   Size:        882     975
 
Bme1580I G_kGCm'C

Cuts at:      0    1501    1857 
   Size:       1501     356
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     400    1857 
   Size:        400    1457
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1857 
   Size:        637    1220
 
Bpu10I CC'TnA_GC

Cuts at:      0     305    1857 
   Size:        305    1552
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1661    1857 
   Size:       1661     196
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     778    1857 
   Size:        778    1079
 
BsaBI GATnn'nnATC

Cuts at:      0     905    1857 
   Size:        905     952
 
BsaWI w'CCGG_w

Cuts at:      0    1148    1857 
   Size:       1148     709
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1110    1140    1596    1626    1857 
   Size:       1110      30     456      30     231

  Fragments arranged by size: 

                1110     456     231      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304    1031    1469    1604    1857 
   Size:        304     727     438     135     253

  Fragments arranged by size: 

                 727     438     304     253     135
 
BseYI C'CCAG_C

Cuts at:      0     561     692    1139    1857 
   Size:        561     131     447     718

  Fragments arranged by size: 

                 718     561     447     131
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1132    1577    1857 
   Size:       1132     445     280

  Fragments arranged by size: 

                1132     445     280
 
BsiEI CG_ry'CG

Cuts at:      0     985    1857 
   Size:        985     872
 
BsiHKAI G_wGCw'C

Cuts at:      0     801    1857 
   Size:        801    1056
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     801    1477    1501    1857 
   Size:        170     631     676      24     356

  Fragments arranged by size: 

                 676     631     356     170      24
 
BspEI T'CCGG_A

Cuts at:      0    1148    1857 
   Size:       1148     709
 
BspHI T'CATG_A

Cuts at:      0     267    1857 
   Size:        267    1590
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     453    1366    1506    1857 
   Size:        453     913     140     351

  Fragments arranged by size: 

                 913     453     351     140
 
BsrDI GCAATG_nn'

Cuts at:      0    1536    1554    1857 
   Size:       1536      18     303

  Fragments arranged by size: 

                1536     303      18
 
BsrFI r'CCGG_y

Cuts at:      0     145     980    1857 
   Size:        145     835     877

  Fragments arranged by size: 

                 877     835     145
 
BstEII G'GTnAC_C

Cuts at:      0    1602    1857 
   Size:       1602     255
 
BstYI r'GATC_y

Cuts at:      0     882     906     933    1068    1365    1384    1857 
   Size:        882      24      27     135     297      19     473

  Fragments arranged by size: 

                 882     473     297     135      27      24      19
 
BtgI C'CryG_G

Cuts at:      0     788    1857 
   Size:        788    1069
 
BtsI GCAGTG_nn'

Cuts at:      0     646     740    1857 
   Size:        646      94    1117

  Fragments arranged by size: 

                1117     646      94
 
DraI TTT'AAA

Cuts at:      0     714     867    1857 
   Size:        714     153     990

  Fragments arranged by size: 

                 990     714     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     158     789    1150    1857 
   Size:        158     631     361     707

  Fragments arranged by size: 

                 707     631     361     158
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     404     464     637     914    1566    1646    1742 
   Size:        227     177      60     173     277     652      80      96

Cuts at:   1742    1857 
   Size:        115

  Fragments arranged by size: 

                 652     277     227     177     173     115      96      80
                  60
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1498    1518    1857 
   Size:       1498      20     339

  Fragments arranged by size: 

                1498     339      20
 
EcoRI G'AATT_C

Cuts at:      0    1044    1857 
   Size:       1044     813
 
HaeII r_GCGC'y

Cuts at:      0     852    1857 
   Size:        852    1005
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1596    1628    1857 
   Size:       1596      32     229

  Fragments arranged by size: 

                1596     229      32
 
HindIII A'AGCT_T

Cuts at:      0     257    1857 
   Size:        257    1600
 
MfeI C'AATT_G

Cuts at:      0     284    1487    1857 
   Size:        284    1203     370

  Fragments arranged by size: 

                1203     370     284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      39    1294    1857 
   Size:         39    1255     563

  Fragments arranged by size: 

                1255     563      39
 
MslI CAynn'nnrTG

Cuts at:      0     415    1857 
   Size:        415    1442
 
MspA1I CmG'CkG

Cuts at:      0    1629    1857 
   Size:       1629     228
 
NcoI C'CATG_G

Cuts at:      0     788    1857 
   Size:        788    1069
 
NspI r_CATG'y

Cuts at:      0     115    1857 
   Size:        115    1742
 
PciI A'CATG_T

Cuts at:      0     111    1857 
   Size:        111    1746
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     746    1857 
   Size:        746    1111
 
PfoI T'CCnGG_A

Cuts at:      0     653    1857 
   Size:        653    1204
 
PpuMI rG'GwC_Cy

Cuts at:      0    1518    1857 
   Size:       1518     339
 
PspOMI G'GGCC_C

Cuts at:      0    1497    1857 
   Size:       1497     360
 
PstI C_TGCA'G

Cuts at:      0    1460    1857 
   Size:       1460     397
 
PvuII CAG'CTG

Cuts at:      0    1629    1857 
   Size:       1629     228
 
SapI GCTCTTCn'nnn_

Cuts at:      0     158     789    1150    1857 
   Size:        158     631     361     707

  Fragments arranged by size: 

                 707     631     361     158
 
ScaI AGT'ACT

Cuts at:      0    1436    1857 
   Size:       1436     421
 
SfcI C'TryA_G

Cuts at:      0    1025    1456    1857 
   Size:       1025     431     401

  Fragments arranged by size: 

                1025     431     401
 
SmlI C'TyrA_G

Cuts at:      0    1676    1857 
   Size:       1676     181
 
StyI C'CwwG_G

Cuts at:      0     788    1297    1857 
   Size:        788     509     560

  Fragments arranged by size: 

                 788     560     509
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     427    1655    1857 
   Size:        427    1228     202

  Fragments arranged by size: 

                1228     427     202
 
TatI w'GTAC_w

Cuts at:      0    1164    1173    1434    1857 
   Size:       1164       9     261     423

  Fragments arranged by size: 

                1164     423     261       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1110    1142    1857 
   Size:       1110      32     715

  Fragments arranged by size: 

                1110     715      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1071    1857 
   Size:       1071     786
 
XmnI GAAnn'nnTTC

Cuts at:      0     113    1857 
   Size:        113    1744
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI     ApoI
    AvrII    BamHI    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BstEII    BstYI     BtgI     BtsI     DraI     EarI
  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I  HindIII     MfeI     MmeI
     MslI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII     SapI     ScaI     SfcI     SmlI     StyI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI     BanI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI    BsaAI    BsaHI    BsiWI     BsmI    BsmBI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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