(Circular) (Six-base) MAPSORT of: pFN21AB9351.seq Check: 1834 from: 1 to: 10537 pFN21AB9351 10537 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7710 7710 Size: 10537 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4945 8347 278 Size: 53 83 186 936 3409 3402 2468 Fragments arranged by size: 3409 3402 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3013 5358 6390 7674 7880 1937 Size: 1076 2345 1032 1284 206 4594 Fragments arranged by size: 4594 2345 1284 1076 1032 206 Acc65I G'GTAC_C Cuts at: 7858 7858 Size: 10537 AclI AA'CG_TT Cuts at: 1563 8664 9037 1563 Size: 7101 373 3063 Fragments arranged by size: 7101 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 4185 4580 5001 5111 5459 7209 7305 Size: 539 2014 395 421 110 348 1750 96 Cuts at: 7305 8600 9984 10252 1632 Size: 1295 1384 268 1917 Fragments arranged by size: 2014 1917 1750 1384 1295 539 421 395 348 268 110 96 AfeI AGC'GCT Cuts at: 2581 2581 Size: 10537 AflII C'TTAA_G Cuts at: 820 1017 4322 820 Size: 197 3305 7035 Fragments arranged by size: 7035 3305 197 AflIII A'CryG_T Cuts at: 1203 3863 6385 9436 10413 1203 Size: 2660 2522 3051 977 1327 Fragments arranged by size: 3051 2660 2522 1327 977 AgeI A'CCGG_T Cuts at: 6801 9368 6801 Size: 2567 7970 AhdI GACnn_n'nnGTC Cuts at: 4622 5878 9266 4622 Size: 1256 3388 5893 Fragments arranged by size: 5893 3388 1256 AleI CACnn'nnGTG Cuts at: 1166 3509 4890 1166 Size: 2343 1381 6813 Fragments arranged by size: 6813 2343 1381 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5642 6424 6492 1184 Size: 4458 782 68 5229 Fragments arranged by size: 5229 4458 782 68 AlwNI CAG_nnn'CTG Cuts at: 2141 4922 5030 7702 8280 9852 2141 Size: 2781 108 2672 578 1572 2826 Fragments arranged by size: 2826 2781 2672 1572 578 108 ApaLI G'TGCA_C Cuts at: 3083 4465 7552 7607 8594 9750 3083 Size: 1382 3087 55 987 1156 3870 Fragments arranged by size: 3870 3087 1382 1156 987 55 ApoI r'AATT_y Cuts at: 1493 2060 3274 3976 4042 4138 4350 6209 6268 Size: 567 1214 702 66 96 212 1859 59 Cuts at: 6268 6361 6473 6736 7302 7532 7754 7846 8072 Size: 93 112 263 566 230 222 92 226 Cuts at: 8072 8224 10333 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1859 1697 1214 702 567 566 263 230 226 222 212 152 112 96 93 92 66 59 AseI AT'TA_AT Cuts at: 160 1032 5081 9091 160 Size: 872 4049 4010 1606 Fragments arranged by size: 4049 4010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10537 AvaI C'yCGr_G Cuts at: 1870 1943 7194 7862 1870 Size: 73 5251 668 4545 Fragments arranged by size: 5251 4545 668 73 BamHI G'GATC_C Cuts at: 2180 7867 8234 2180 Size: 5687 367 4483 Fragments arranged by size: 5687 4483 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5553 5657 6825 7858 9313 Size: 325 217 627 3766 104 1168 1033 1455 Cuts at: 9313 618 Size: 1842 Fragments arranged by size: 3766 1842 1455 1168 1033 627 325 217 104 BanII G_rGCy'C Cuts at: 1391 5175 6172 6852 7518 7856 1391 Size: 3784 997 680 666 338 4072 Fragments arranged by size: 4072 3784 997 680 666 338 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2145 7405 928 Size: 971 246 5260 4060 Fragments arranged by size: 5260 4060 971 246 BbvCI CC'TCA_GC Cuts at: 7619 7619 Size: 10537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2067 2101 6602 6636 8728 8762 1903 Size: 34 130 34 4501 34 2092 34 3678 Fragments arranged by size: 4501 3678 2092 130 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8429 9639 8429 Size: 1210 9327 BclI T'GATC_A Cuts at: 1548 1548 Size: 10537 BfrBI ATG'CAT Cuts at: 2454 2488 4498 6025 2454 Size: 34 2010 1527 6966 Fragments arranged by size: 6966 2010 1527 34 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 9148 136 Size: 107 122 71 631 605 7476 1525 Fragments arranged by size: 7476 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4181 6146 10532 4181 Size: 1965 4386 4186 Fragments arranged by size: 4386 4186 1965 BlpI GC'TnA_GC Cuts at: 4027 7649 7954 4027 Size: 3622 305 6610 Fragments arranged by size: 6610 3622 305 Bme1580I G_kGCm'C Cuts at: 1790 3087 4469 5338 5556 6766 6926 7556 7611 Size: 1297 1382 869 218 1210 160 630 55 Cuts at: 7611 8598 9754 1790 Size: 987 1156 2573 Fragments arranged by size: 2573 1382 1297 1210 1156 987 869 630 218 160 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10537 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3003 3582 3665 Size: 416 53 83 399 24 1586 579 83 Cuts at: 3665 3951 4132 5337 7033 8268 9226 442 Size: 286 181 1205 1696 1235 958 1753 Fragments arranged by size: 1753 1696 1586 1235 1205 958 579 416 399 286 181 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10537 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5902 7815 5902 Size: 1913 8624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2586 3338 3930 6797 9197 1239 Size: 1347 752 592 2867 2400 2579 Fragments arranged by size: 2867 2579 2400 1347 752 592 Bpu10I CC'TnA_GC Cuts at: 5890 5970 6221 6592 7506 7619 5890 Size: 80 251 371 914 113 8808 Fragments arranged by size: 8808 914 371 251 113 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4648 8662 9527 9825 4648 Size: 4014 865 298 5360 Fragments arranged by size: 5360 4014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2073 7343 9200 882 Size: 617 574 5270 1857 2219 Fragments arranged by size: 5270 2219 1857 617 574 BsaAI yAC'GTr Cuts at: 493 1204 8328 493 Size: 711 7124 2702 Fragments arranged by size: 7124 2702 711 BsaBI GATnn'nnATC Cuts at: 10531 10531 Size: 10537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4942 8344 8726 9361 Size: 53 83 186 936 3409 3402 382 635 Cuts at: 9361 275 Size: 1451 Fragments arranged by size: 3409 3402 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2817 6801 8970 9368 9642 9789 10200 2817 Size: 3984 2169 398 274 147 411 3154 Fragments arranged by size: 3984 3154 2169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2247 2277 4310 4340 1178 Size: 30 165 30 844 30 2033 30 7375 Fragments arranged by size: 7375 2033 844 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2962 4331 5048 5331 6723 1186 Size: 1776 1369 717 283 1392 5000 Fragments arranged by size: 5000 1776 1392 1369 717 283 BseYI C'CCAG_C Cuts at: 3348 3396 5403 6781 9740 10236 3348 Size: 48 2007 1378 2959 496 3649 Fragments arranged by size: 3649 2959 2007 1378 496 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 5126 5180 5851 6489 1163 Size: 573 894 2496 54 671 638 5211 Fragments arranged by size: 5211 2496 894 671 638 573 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7987 8748 9776 1540 Size: 275 186 5986 761 1028 2301 Fragments arranged by size: 5986 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3064 3087 3946 4469 5001 5175 6350 6731 7556 Size: 23 859 523 532 174 1175 381 825 Cuts at: 7556 7611 7730 8598 8683 9754 3064 Size: 55 119 868 85 1071 3847 Fragments arranged by size: 3847 1175 1071 868 859 825 532 523 381 174 119 85 55 23 BsmI GAATG_Cn' Cuts at: 2800 4578 4759 5691 6027 7223 8048 8141 2800 Size: 1778 181 932 336 1196 825 93 5196 Fragments arranged by size: 5196 1778 1196 932 825 336 181 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3064 3087 3946 4469 5001 5175 5338 Size: 399 1274 23 859 523 532 174 163 Cuts at: 5338 5556 6172 6350 6731 6766 6852 6926 7518 Size: 218 616 178 381 35 86 74 592 Cuts at: 7518 7556 7611 7730 7856 8598 8683 9754 1391 Size: 38 55 119 126 742 85 1071 2174 Fragments arranged by size: 2174 1274 1071 859 742 616 592 532 523 399 381 218 178 174 163 126 119 86 85 74 55 38 35 23 BspEI T'CCGG_A Cuts at: 2817 10200 2817 Size: 7383 3154 BspHI T'CATG_A Cuts at: 3703 3996 8426 10156 3703 Size: 293 4430 1730 4084 Fragments arranged by size: 4430 4084 1730 293 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4291 7710 7892 844 Size: 3447 3419 182 3489 Fragments arranged by size: 3489 3447 3419 182 BsrBI CCG'CTC Cuts at: 1394 1823 6752 7489 8424 1394 Size: 429 4929 737 935 3507 Fragments arranged by size: 4929 3507 935 737 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2443 2739 4074 5300 6295 6744 7386 Size: 1183 1195 296 1335 1226 995 449 642 Cuts at: 7386 9032 9206 65 Size: 1646 174 1396 Fragments arranged by size: 1646 1396 1335 1226 1195 1183 995 642 449 296 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6801 9181 9368 1700 Size: 110 4991 2380 187 2869 Fragments arranged by size: 4991 2869 2380 187 110 BsrGI T'GTAC_A Cuts at: 96 4641 96 Size: 4545 5992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10537 BssSI C'ACGA_G Cuts at: 2204 5066 5183 8597 9609 2204 Size: 2862 117 3414 1012 3132 Fragments arranged by size: 3414 3132 2862 1012 117 BstAPI GCAn_nnn'nTGC Cuts at: 794 3437 5113 5919 6512 794 Size: 2643 1676 806 593 4819 Fragments arranged by size: 4819 2643 1676 806 593 BstBI TT'CG_AA Cuts at: 9341 9341 Size: 10537 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10537 BstXI CCAn_nnnn'nTGG Cuts at: 3743 5731 6082 6583 7023 7146 3743 Size: 1988 351 501 440 123 7134 Fragments arranged by size: 7134 1988 501 440 351 123 BstYI r'GATC_y Cuts at: 1972 2180 3769 4109 4181 4948 6146 7135 7309 Size: 208 1589 340 72 767 1198 989 174 Cuts at: 7309 7867 8234 8619 8636 10088 10174 10186 10532 Size: 558 367 385 17 1452 86 12 346 Cuts at: 10532 1972 Size: 1977 Fragments arranged by size: 1977 1589 1452 1198 989 767 558 385 367 346 340 208 174 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 3813 5661 6576 6843 513 Size: 552 2748 1848 915 267 4207 Fragments arranged by size: 4207 2748 1848 915 552 267 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9798 Fragments arranged by size: 9798 683 56 BtsI GCAGTG_nn' Cuts at: 2083 3490 5769 8053 8847 8867 2083 Size: 1407 2279 2284 794 20 3753 Fragments arranged by size: 3753 2284 2279 1407 794 20 ClaI AT'CG_AT Cuts at: 1554 5379 1554 Size: 3825 6712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10502 DraI TTT'AAA Cuts at: 2853 6993 7841 2853 Size: 4140 848 5549 Fragments arranged by size: 5549 4140 848 DraIII CAC_nnn'GTG Cuts at: 4309 6930 7684 4309 Size: 2621 754 7162 Fragments arranged by size: 7162 2621 754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6499 9544 809 Size: 507 5183 3045 1802 Fragments arranged by size: 5183 3045 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2889 4913 5508 Size: 54 1425 50 275 18 1059 2024 595 Cuts at: 5508 6840 7984 8873 10502 8 Size: 1332 1144 889 1629 43 Fragments arranged by size: 2024 1629 1425 1332 1144 1059 889 595 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7984 1537 Size: 275 6172 4090 Fragments arranged by size: 6172 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 4848 4866 5247 6759 7405 7754 8467 Size: 1479 2016 18 381 1512 646 349 713 Cuts at: 8467 1353 Size: 3423 Fragments arranged by size: 3423 2016 1512 1479 713 646 381 349 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9124 9498 9644 387 Size: 1064 138 7535 374 146 1280 Fragments arranged by size: 7535 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5173 5173 Size: 10537 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2586 3338 3930 4185 4580 5001 Size: 393 539 415 752 592 255 395 421 Cuts at: 5001 5111 5459 6797 7209 7305 8600 9197 9984 Size: 110 348 1338 412 96 1295 597 787 Cuts at: 9984 10252 1239 Size: 268 1524 Fragments arranged by size: 1524 1338 1295 787 752 597 592 539 421 415 412 395 393 348 268 255 110 96 EcoNI CCTnn'n_nnAGG Cuts at: 2189 3075 7079 2189 Size: 886 4004 5647 Fragments arranged by size: 5647 4004 886 EcoO109I rG'GnC_Cy Cuts at: 3953 4403 6527 3953 Size: 450 2124 7963 Fragments arranged by size: 7963 2124 450 EcoRI G'AATT_C Cuts at: 7532 7846 8224 7532 Size: 314 378 9845 Fragments arranged by size: 9845 378 314 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2601 4079 6738 2601 Size: 1478 2659 6400 Fragments arranged by size: 6400 2659 1478 FspI TGC'GCA Cuts at: 5136 9043 5136 Size: 3907 6630 FspAI rTGC'GCAy Cuts at: 5136 5136 Size: 10537 HaeII r_GCGC'y Cuts at: 1678 2583 5450 6010 9684 1678 Size: 905 2867 560 3674 2531 Fragments arranged by size: 3674 2867 2531 905 560 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3759 3791 4094 4126 4540 Size: 32 135 32 2187 32 303 32 414 Cuts at: 4540 4572 4608 4640 4664 4696 4782 4814 4927 Size: 32 36 32 24 32 86 32 113 Cuts at: 4927 4959 5060 5092 5122 5154 5864 5896 6217 Size: 32 101 32 30 32 710 32 321 Cuts at: 6217 6249 9178 9210 9252 9284 1373 Size: 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 2187 710 414 321 303 135 113 101 86 42 36 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 30 24 HincII GTy'rAC Cuts at: 669 1657 1938 2362 5152 6283 7881 8127 669 Size: 988 281 424 2790 1131 1598 246 3079 Fragments arranged by size: 3079 2790 1598 1131 988 424 281 246 HindIII A'AGCT_T Cuts at: 748 4206 7732 748 Size: 3458 3526 3553 Fragments arranged by size: 3553 3526 3458 HpaI GTT'AAC Cuts at: 8127 8127 Size: 10537 KpnI G_GTAC'C Cuts at: 7862 7862 Size: 10537 MfeI C'AATT_G Cuts at: 3210 4470 5219 8136 3210 Size: 1260 749 2917 5611 Fragments arranged by size: 5611 2917 1260 749 MluI A'CGCG_T Cuts at: 10413 10413 Size: 10537 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3063 3594 3760 5315 5974 6171 9651 9835 Size: 1758 531 166 1555 659 197 3480 184 Cuts at: 9835 1305 Size: 2007 Fragments arranged by size: 3480 2007 1758 1555 659 531 197 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 4915 5510 10 Size: 54 1425 343 1059 2024 595 5037 Fragments arranged by size: 5037 2024 1425 1059 595 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2100 2386 3509 4001 4155 4890 5083 Size: 648 934 286 1123 492 154 735 193 Cuts at: 5083 5381 5421 5666 6080 6581 6831 7379 8495 Size: 298 40 245 414 501 250 548 1116 Cuts at: 8495 8854 9013 518 Size: 359 159 2042 Fragments arranged by size: 2042 1123 1116 934 735 648 548 501 492 414 359 298 286 250 245 193 159 154 40 MspA1I CmG'CkG Cuts at: 1606 2128 6845 7623 7648 7953 8630 9778 10023 Size: 522 4717 778 25 305 677 1148 245 Cuts at: 10023 1606 Size: 2120 Fragments arranged by size: 4717 2120 1148 778 677 522 305 245 25 NcoI C'CATG_G Cuts at: 513 1065 3813 5661 6576 513 Size: 552 2748 1848 915 4474 Fragments arranged by size: 4474 2748 1848 915 552 NdeI CA'TA_TG Cuts at: 387 2456 387 Size: 2069 8468 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10537 NotI GC'GGCC_GC Cuts at: 7984 7984 Size: 10537 NsiI A_TGCA'T Cuts at: 2456 2490 4500 6027 2456 Size: 34 2010 1527 6966 Fragments arranged by size: 6966 2010 1527 34 NspI r_CATG'y Cuts at: 1132 1229 2488 2644 5501 6389 7657 7895 9440 Size: 97 1259 156 2857 888 1268 238 1545 Cuts at: 9440 1132 Size: 2229 Fragments arranged by size: 2857 2229 1545 1268 1259 888 238 156 97 PasI CC'CwG_GG Cuts at: 1348 5908 1348 Size: 4560 5977 PciI A'CATG_T Cuts at: 6385 9436 6385 Size: 3051 7486 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 3602 6682 1108 Size: 1227 1267 3080 4963 Fragments arranged by size: 4963 3080 1267 1227 PfoI T'CCnGG_A Cuts at: 3538 3946 3538 Size: 408 10129 PmeI GTTT'AAAC Cuts at: 7841 7841 Size: 10537 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5224 5256 8606 8638 10119 10151 5224 Size: 32 3350 32 1481 32 5610 Fragments arranged by size: 5610 3350 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3953 6527 3953 Size: 2574 7963 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10537 PsiI TTA'TAA Cuts at: 2346 4014 6305 8107 2346 Size: 1668 2291 1802 4776 Fragments arranged by size: 4776 2291 1802 1668 PstI C_TGCA'G Cuts at: 830 2133 5764 5815 7497 7889 830 Size: 1303 3631 51 1682 392 3478 Fragments arranged by size: 3631 3478 1682 1303 392 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10537 PvuII CAG'CTG Cuts at: 2128 7648 2128 Size: 5520 5017 SacI G_AGCT'C Cuts at: 5175 5175 Size: 10537 SacII CC_GC'GG Cuts at: 6846 6846 Size: 10537 SalI G'TCGA_C Cuts at: 1936 7879 1936 Size: 5943 4594 SapI GCTCTTCn'nnn_ Cuts at: 4866 5247 6759 7754 4866 Size: 381 1512 995 7649 Fragments arranged by size: 7649 1512 995 381 SbfI CC_TGCA'GG Cuts at: 7889 7889 Size: 10537 ScaI AGT'ACT Cuts at: 3592 3613 7326 8785 10497 3592 Size: 21 3713 1459 1712 3632 Fragments arranged by size: 3713 3632 1712 1459 21 SexAI A'CCwGG_T Cuts at: 3492 3492 Size: 10537 SfcI C'TryA_G Cuts at: 826 1045 2129 2428 4423 4704 5247 5760 5811 Size: 219 1084 299 1995 281 543 513 51 Cuts at: 5811 7493 7710 7885 9020 9701 9892 826 Size: 1682 217 175 1135 681 191 1471 Fragments arranged by size: 1995 1682 1471 1135 1084 681 543 513 299 281 219 217 191 175 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10537 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10537 SmaI CCC'GGG Cuts at: 1872 7196 7864 1872 Size: 5324 668 4545 Fragments arranged by size: 5324 4545 668 SmlI C'TyrA_G Cuts at: 820 1017 1943 4322 4627 8641 9542 9804 820 Size: 197 926 2379 305 4014 901 262 1553 Fragments arranged by size: 4014 2379 1553 926 901 305 262 197 SnaBI TAC'GTA Cuts at: 493 8328 493 Size: 7835 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10537 SphI G_CATG'C Cuts at: 1132 2644 5501 7657 7895 1132 Size: 1512 2857 2156 238 3774 Fragments arranged by size: 3774 2857 2156 1512 238 SrfI GCCC'GGGC Cuts at: 7196 7196 Size: 10537 SspI AAT'ATT Cuts at: 5 52 4130 7262 8461 5 Size: 47 4078 3132 1199 2081 Fragments arranged by size: 4078 3132 2081 1199 47 StuI AGG'CCT Cuts at: 1517 2426 4096 4964 1517 Size: 909 1670 868 7090 Fragments arranged by size: 7090 1670 909 868 StyI C'CwwG_G Cuts at: 513 1065 3637 3813 5598 5661 6576 6688 7080 Size: 552 2572 176 1785 63 915 112 392 Cuts at: 7080 7632 7976 513 Size: 552 344 3074 Fragments arranged by size: 3074 2572 1785 915 552 552 392 344 176 112 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2626 2664 3022 4534 8564 8581 8734 Size: 1255 1046 38 358 1512 4030 17 153 Cuts at: 8734 8919 9364 325 Size: 185 445 1498 Fragments arranged by size: 4030 1512 1498 1255 1046 445 358 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3590 3611 3728 Size: 274 80 33 51 1443 1613 21 117 Cuts at: 3728 3856 4641 5985 7324 8783 10495 96 Size: 128 785 1344 1339 1459 1712 138 Fragments arranged by size: 1712 1613 1459 1443 1344 1339 785 274 138 128 117 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 2286 2318 3480 3512 4459 4491 7052 Size: 32 7 32 1162 32 947 32 2561 Cuts at: 7052 7084 7219 7251 10497 10529 2247 Size: 32 135 32 3246 32 2255 Fragments arranged by size: 3246 2561 2255 1162 947 135 32 32 32 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 3565 1316 Size: 2249 8288 XbaI T'CTAG_A Cuts at: 7873 7873 Size: 10537 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3404 3472 6187 6273 6686 3404 Size: 68 2715 86 413 7255 Fragments arranged by size: 7255 2715 413 86 68 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10537 XmaI C'CCGG_G Cuts at: 1870 7194 7862 1870 Size: 5324 668 4545 Fragments arranged by size: 5324 4545 668 XmnI GAAnn'nnTTC Cuts at: 2064 2581 4354 4842 8666 2064 Size: 517 1773 488 3824 3935 Fragments arranged by size: 3935 3824 1773 517 488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4943 8345 276 Size: 53 83 186 936 3409 3402 2468 Fragments arranged by size: 3409 3402 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SwaI