Restriction map for
pFN21AB9098
(Linear) (Six-base) MAPSORT of: KIBB9098.seq Check: 9164 from: 1 to: 3771
KIBB9098.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 443 3771
Size: 443 3328
AatII G_ACGT'C
Cuts at: 0 857 3771
Size: 857 2914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 541 940 1099 1851 2310 2414 2555
Size: 279 262 399 159 752 459 104 141
Cuts at: 2555 3050 3218 3771
Size: 495 168 553
Fragments arranged by size:
752 553 495 459 399 279 262 168
159 141 104
AfeI AGC'GCT
Cuts at: 0 2435 3771
Size: 2435 1336
AhdI GACnn_n'nnGTC
Cuts at: 0 2618 3771
Size: 2618 1153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1912 3771
Size: 1912 1859
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3746 3771
Size: 3746 25
AlwNI CAG_nnn'CTG
Cuts at: 0 147 774 1038 3771
Size: 147 627 264 2733
Fragments arranged by size:
2733 627 264 147
ApoI r'AATT_y
Cuts at: 0 1794 2565 3771
Size: 1794 771 1206
Fragments arranged by size:
1794 1206 771
AseI AT'TA_AT
Cuts at: 0 415 3771
Size: 415 3356
AvaI C'yCGr_G
Cuts at: 0 171 186 3771
Size: 171 15 3585
Fragments arranged by size:
3585 171 15
BamHI G'GATC_C
Cuts at: 0 11 1612 3771
Size: 11 1601 2159
Fragments arranged by size:
2159 1601 11
BanI G'GyrC_C
Cuts at: 0 1395 1430 2698 3771
Size: 1395 35 1268 1073
Fragments arranged by size:
1395 1268 1073 35
BanII G_rGCy'C
Cuts at: 0 178 1184 1772 2087 2452 2876 2927 3050
Size: 178 1006 588 315 365 424 51 123
Cuts at: 3050 3771
Size: 721
Fragments arranged by size:
1006 721 588 424 365 315 178 123
51
BbeI G_GCGC'C
Cuts at: 0 1434 3771
Size: 1434 2337
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 602 673 1992 2429 3102 3584 3731
Size: 466 136 71 1319 437 673 482 147
Cuts at: 3731 3771
Size: 40
Fragments arranged by size:
1319 673 482 466 437 147 136 71
40
BbvCI CC'TCA_GC
Cuts at: 0 325 752 2275 3771
Size: 325 427 1523 1496
Fragments arranged by size:
1523 1496 427 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1311 1345 2871 2905 3771
Size: 1311 34 1526 34 866
Fragments arranged by size:
1526 1311 866 34 34
BclI T'GATC_A
Cuts at: 0 1325 1820 2484 3771
Size: 1325 495 664 1287
Fragments arranged by size:
1325 1287 664 495
BglI GCCn_nnn'nGGC
Cuts at: 0 189 2653 3771
Size: 189 2464 1118
Fragments arranged by size:
2464 1118 189
BglII A'GATC_T
Cuts at: 0 902 3771
Size: 902 2869
BlpI GC'TnA_GC
Cuts at: 0 2437 3044 3771
Size: 2437 607 727
Fragments arranged by size:
2437 727 607
Bme1580I G_kGCm'C
Cuts at: 0 1400 2701 3771
Size: 1400 1301 1070
Fragments arranged by size:
1400 1301 1070
BmgBI CAC'GTC
Cuts at: 0 33 3392 3771
Size: 33 3359 379
Fragments arranged by size:
3359 379 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 2841 3771
Size: 2841 930
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 3366 3771
Size: 186 3180 405
Fragments arranged by size:
3180 405 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 286 766 918 1141 1267 1538 1951
Size: 223 63 480 152 223 126 271 413
Cuts at: 1951 2101 3028 3091 3361 3771
Size: 150 927 63 270 410
Fragments arranged by size:
927 480 413 410 271 270 223 223
152 150 126 63 63
Bpu10I CC'TnA_GC
Cuts at: 0 85 325 752 1957 2275 2733 3771
Size: 85 240 427 1205 318 458 1038
Fragments arranged by size:
1205 1038 458 427 318 240 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 3771
Size: 529 3242
BsaI GGTCTCn'nnnn_
Cuts at: 0 1117 1445 2854 3771
Size: 1117 328 1409 917
Fragments arranged by size:
1409 1117 917 328
BsaAI yAC'GTr
Cuts at: 0 765 3771
Size: 765 3006
BsaBI GATnn'nnATC
Cuts at: 0 508 2483 3771
Size: 508 1975 1288
Fragments arranged by size:
1975 1288 508
BsaHI Gr'CG_yC
Cuts at: 0 854 1431 1832 3562 3771
Size: 854 577 401 1730 209
Fragments arranged by size:
1730 854 577 401 209
BsaWI w'CCGG_w
Cuts at: 0 689 1298 2720 3771
Size: 689 609 1422 1051
Fragments arranged by size:
1422 1051 689 609
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1240 1270 2074 2104 2517 2547 3746 3771
Size: 1240 30 804 30 413 30 1199 25
Fragments arranged by size:
1240 1199 804 413 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1123 1264 1594 1612 2154 2485 3025 3145
Size: 1123 141 330 18 542 331 540 120
Cuts at: 3145 3568 3771
Size: 423 203
Fragments arranged by size:
1123 542 540 423 331 330 203 141
120 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 646 760 787 1551 1969 2762 3771
Size: 415 231 114 27 764 418 793 1009
Fragments arranged by size:
1009 793 764 418 415 231 114 27
BsiEI CG_ry'CG
Cuts at: 0 222 2657 3771
Size: 222 2435 1114
Fragments arranged by size:
2435 1114 222
BsiHKAI G_wGCw'C
Cuts at: 0 178 1184 1208 2087 2452 2825 2876 2927
Size: 178 1006 24 879 365 373 51 51
Cuts at: 2927 3671 3771
Size: 744 100
Fragments arranged by size:
1006 879 744 373 365 178 100 51
51 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1279 3771
Size: 1279 2492
Bsp1286I G_dGCh'C
Cuts at: 0 178 1184 1208 1400 1772 2087 2452 2701
Size: 178 1006 24 192 372 315 365 249
Cuts at: 2701 2825 2876 2927 3050 3671 3771
Size: 124 51 51 123 621 100
Fragments arranged by size:
1006 621 372 365 315 249 192 178
124 123 100 51 51 24
BspEI T'CCGG_A
Cuts at: 0 1298 3771
Size: 1298 2473
BspHI T'CATG_A
Cuts at: 0 1200 1772 2609 3188 3771
Size: 1200 572 837 579 583
Fragments arranged by size:
1200 837 583 579 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 206 437 443 1955 3771
Size: 155 51 231 6 1512 1816
Fragments arranged by size:
1816 1512 231 155 51 6
BsrBI CCG'CTC
Cuts at: 0 516 1923 2940 3771
Size: 516 1407 1017 831
Fragments arranged by size:
1407 1017 831 516
BsrDI GCAATG_nn'
Cuts at: 0 368 1770 3255 3771
Size: 368 1402 1485 516
Fragments arranged by size:
1485 1402 516 368
BsrFI r'CCGG_y
Cuts at: 0 240 653 2210 3771
Size: 240 413 1557 1561
Fragments arranged by size:
1561 1557 413 240
BssHII G'CGCG_C
Cuts at: 0 55 224 2145 3771
Size: 55 169 1921 1626
Fragments arranged by size:
1921 1626 169 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 370 438 774 3771
Size: 147 223 68 336 2997
Fragments arranged by size:
2997 336 223 147 68
BstEII G'GTnAC_C
Cuts at: 0 692 3771
Size: 692 3079
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2079 2767 3771
Size: 2079 688 1004
Fragments arranged by size:
2079 1004 688
BstYI r'GATC_y
Cuts at: 0 11 902 1034 1151 1343 1612 1901 3771
Size: 11 891 132 117 192 269 289 1870
Fragments arranged by size:
1870 891 289 269 192 132 117 11
BtgI C'CryG_G
Cuts at: 0 464 3771
Size: 464 3307
BtsI GCAGTG_nn'
Cuts at: 0 1044 2649 3372 3771
Size: 1044 1605 723 399
Fragments arranged by size:
1605 1044 723 399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 106 141 3771
Size: 106 35 3630
Fragments arranged by size:
3630 106 35
DraI TTT'AAA
Cuts at: 0 1644 3771
Size: 1644 2127
DraIII CAC_nnn'GTG
Cuts at: 0 2835 3508 3771
Size: 2835 673 263
Fragments arranged by size:
2835 673 263
DrdI GACnn_nn'nnGTC
Cuts at: 0 3586 3771
Size: 3586 185
EaeI y'GGCC_r
Cuts at: 0 27 251 467 815 1310 1617 2100 2267
Size: 27 224 216 348 495 307 483 167
Cuts at: 2267 2312 2654 3293 3771
Size: 45 342 639 478
Fragments arranged by size:
639 495 483 478 348 342 307 224
216 167 45 27
EagI C'GGCC_G
Cuts at: 0 2654 3771
Size: 2654 1117
EarI CTCTTCn'nnn_
Cuts at: 0 157 3771
Size: 157 3614
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 672 2229 2815 3771
Size: 672 1557 586 956
Fragments arranged by size:
1557 956 672 586
EcoICRI GAG'CTC
Cuts at: 0 176 1182 2085 2450 2874 2925 3771
Size: 176 1006 903 365 424 51 846
Fragments arranged by size:
1006 903 846 424 365 176 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 279 286 541 766 918 940 1099
Size: 223 56 7 255 225 152 22 159
Cuts at: 1099 1141 1267 1538 1851 1951 2101 2310 2414
Size: 42 126 271 313 100 150 209 104
Cuts at: 2414 2555 3028 3050 3091 3218 3361 3771
Size: 141 473 22 41 127 143 410
Fragments arranged by size:
473 410 313 271 255 225 223 209
159 152 150 143 141 127 126 104
100 56 42 41 22 22 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 202 544 1390 2932 3771
Size: 202 342 846 1542 839
Fragments arranged by size:
1542 846 839 342 202
EcoO109I rG'GnC_Cy
Cuts at: 0 389 2256 2982 3176 3771
Size: 389 1867 726 194 595
Fragments arranged by size:
1867 726 595 389 194
EcoRV GAT'ATC
Cuts at: 0 3614 3771
Size: 3614 157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1548 3197 3771
Size: 1548 1649 574
Fragments arranged by size:
1649 1548 574
FspI TGC'GCA
Cuts at: 0 715 3771
Size: 715 3056
HaeII r_GCGC'y
Cuts at: 0 247 1434 2437 3278 3771
Size: 247 1187 1003 841 493
Fragments arranged by size:
1187 1003 841 493 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 520 552 1240 1272 2604 2636
Size: 160 32 328 32 688 32 1332 32
Cuts at: 2636 3771
Size: 1135
Fragments arranged by size:
1332 1135 688 328 160 32 32 32
32
HincII GTy'rAC
Cuts at: 0 789 1857 3591 3771
Size: 789 1068 1734 180
Fragments arranged by size:
1734 1068 789 180
HindIII A'AGCT_T
Cuts at: 0 1525 3419 3442 3771
Size: 1525 1894 23 329
Fragments arranged by size:
1894 1525 329 23
KasI G'GCGC_C
Cuts at: 0 1430 3771
Size: 1430 2341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1853 2560 3771
Size: 1853 707 1211
Fragments arranged by size:
1853 1211 707
MscI TGG'CCA
Cuts at: 0 29 469 1312 2102 3771
Size: 29 440 843 790 1669
Fragments arranged by size:
1669 843 790 440 29
MslI CAynn'nnrTG
Cuts at: 0 1393 1690 2302 3022 3209 3771
Size: 1393 297 612 720 187 562
Fragments arranged by size:
1393 720 612 562 297 187
MspA1I CmG'CkG
Cuts at: 0 264 774 807 938 2279 3087 3314 3771
Size: 264 510 33 131 1341 808 227 457
Fragments arranged by size:
1341 808 510 457 264 227 131 33
NaeI GCC'GGC
Cuts at: 0 242 655 2212 3771
Size: 242 413 1557 1559
Fragments arranged by size:
1559 1557 413 242
NarI GG'CG_CC
Cuts at: 0 1431 3771
Size: 1431 2340
NdeI CA'TA_TG
Cuts at: 0 3307 3771
Size: 3307 464
NgoMIV G'CCGG_C
Cuts at: 0 240 653 2210 3771
Size: 240 413 1557 1561
Fragments arranged by size:
1561 1557 413 240
NspI r_CATG'y
Cuts at: 0 379 714 744 2307 3771
Size: 379 335 30 1563 1464
Fragments arranged by size:
1563 1464 379 335 30
PasI CC'CwG_GG
Cuts at: 0 42 3771
Size: 42 3729
PflMI CCAn_nnn'nTGG
Cuts at: 0 1746 3771
Size: 1746 2025
PfoI T'CCnGG_A
Cuts at: 0 677 1349 2021 2897 3771
Size: 677 672 672 876 874
Fragments arranged by size:
876 874 677 672 672
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1365 1397 3746 3771
Size: 1365 32 2349 25
Fragments arranged by size:
2349 1365 32 25
PpuMI rG'GwC_Cy
Cuts at: 0 389 2982 3176 3771
Size: 389 2593 194 595
Fragments arranged by size:
2593 595 389 194
PspXI vC'TCGA_Gb
Cuts at: 0 171 3771
Size: 171 3600
PstI C_TGCA'G
Cuts at: 0 143 443 1034 1901 2952 3771
Size: 143 300 591 867 1051 819
Fragments arranged by size:
1051 867 819 591 300 143
PvuII CAG'CTG
Cuts at: 0 774 938 2279 3087 3771
Size: 774 164 1341 808 684
Fragments arranged by size:
1341 808 774 684 164
SacI G_AGCT'C
Cuts at: 0 178 1184 2087 2452 2876 2927 3771
Size: 178 1006 903 365 424 51 844
Fragments arranged by size:
1006 903 844 424 365 178 51
SapI GCTCTTCn'nnn_
Cuts at: 0 157 3771
Size: 157 3614
SexAI A'CCwGG_T
Cuts at: 0 725 3771
Size: 725 3046
SfcI C'TryA_G
Cuts at: 0 139 384 439 1030 1897 2948 3771
Size: 139 245 55 591 867 1051 823
Fragments arranged by size:
1051 867 823 591 245 139 55
SfoI GGC'GCC
Cuts at: 0 1432 3771
Size: 1432 2339
SgrAI Cr'CCGG_yG
Cuts at: 0 2210 3771
Size: 2210 1561
SmlI C'TyrA_G
Cuts at: 0 171 544 3771
Size: 171 373 3227
Fragments arranged by size:
3227 373 171
SphI G_CATG'C
Cuts at: 0 714 744 3771
Size: 714 30 3027
Fragments arranged by size:
3027 714 30
SspI AAT'ATT
Cuts at: 0 3691 3771
Size: 3691 80
StuI AGG'CCT
Cuts at: 0 917 3771
Size: 917 2854
StyI C'CwwG_G
Cuts at: 0 254 317 912 1799 3771
Size: 254 63 595 887 1972
Fragments arranged by size:
1972 887 595 254 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 348 586 2559 2823 3279 3771
Size: 348 238 1973 264 456 492
Fragments arranged by size:
1973 492 456 348 264 238
TatI w'GTAC_w
Cuts at: 0 1022 2306 3771
Size: 1022 1284 1465
Fragments arranged by size:
1465 1284 1022
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 54 2821 2853 3494 3526 3771
Size: 22 32 2767 32 641 32 245
Fragments arranged by size:
2767 641 245 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 0 853 2196 3771
Size: 853 1343 1575
Fragments arranged by size:
1575 1343 853
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 951 1242 1431 2013 2019 2500 3771
Size: 261 690 291 189 582 6 481 1271
Fragments arranged by size:
1271 690 582 481 291 261 189 6
XhoI C'TCGA_G
Cuts at: 0 171 3771
Size: 171 3600
ZraI GAC'GTC
Cuts at: 0 855 3771
Size: 855 2916
Enzymes that do cut:
AarI AatII AcuI AfeI AhdI AlfI AloI AlwNI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI
PspXI PstI PvuII SacI SapI SexAI SfcI SfoI
SgrAI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AleI ApaI
ApaLI AscI AsiSI AvrII BaeI BciVI BfrBI BmtI
BseYI BsiWI BsmI BsrGI BssSI BstBI BstZ17I Bsu36I
BtgZI ClaI EcoRI FseI FspAI HpaI KpnI MfeI
MluI NcoI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SgfI SmaI
SnaBI SpeI SrfI SwaI XbaI XmaI XmnI