Restriction map for pFN21AB9075
 (Linear) (Six-base) MAPSORT of: KIBB9075.seq  Check: 2873  from: 1  to: 4014



KIBB9075.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1014    4014 
   Size:       1014    3000
 
AccI GT'mk_AC

Cuts at:      0     396    1922    4014 
   Size:        396    1526    2092

  Fragments arranged by size: 

                2092    1526     396
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1208    3622    3784    4014 
   Size:        467     741    2414     162     230

  Fragments arranged by size: 

                2414     741     467     230     162
 
AfeI AGC'GCT

Cuts at:      0    3769    4014 
   Size:       3769     245
 
AflIII A'CryG_T

Cuts at:      0    3242    4014 
   Size:       3242     772
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3246    4014 
   Size:       3246     768
 
AleI CACnn'nnGTG

Cuts at:      0     973    1660    2296    2559    4014 
   Size:        973     687     636     263    1455

  Fragments arranged by size: 

                1455     973     687     636     263
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2507    2539    4014 
   Size:       2507      32    1475

  Fragments arranged by size: 

                2507    1475      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      11     144     364    3875    4014 
   Size:         11     133     220    3511     139

  Fragments arranged by size: 

                3511     220     139     133      11
 
ApoI r'AATT_y

Cuts at:      0    1583    4014 
   Size:       1583    2431
 
AvaI C'yCGr_G

Cuts at:      0    2062    2468    3131    4014 
   Size:       2062     406     663     883

  Fragments arranged by size: 

                2062     883     663     406
 
BanI G'GyrC_C

Cuts at:      0     112    1403    1894    4014 
   Size:        112    1291     491    2120

  Fragments arranged by size: 

                2120    1291     491     112
 
BanII G_rGCy'C

Cuts at:      0     461    2215    2905    2978    3363    4014 
   Size:        461    1754     690      73     385     651

  Fragments arranged by size: 

                1754     690     651     461     385      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2348    3032    4014 
   Size:       2348     684     982

  Fragments arranged by size: 

                2348     982     684
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1070    1104    2878    2912    4014 
   Size:       1070      34    1774      34    1102

  Fragments arranged by size: 

                1774    1102    1070      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1384    4014 
   Size:       1384    2630
 
BclI T'GATC_A

Cuts at:      0    2004    2276    4014 
   Size:       2004     272    1738

  Fragments arranged by size: 

                2004    1738     272
 
BglII A'GATC_T

Cuts at:      0    2991    4014 
   Size:       2991    1023
 
BlpI GC'TnA_GC

Cuts at:      0      43     220    1219    3935    4014 
   Size:         43     177     999    2716      79

  Fragments arranged by size: 

                2716     999     177      79      43
 
Bme1580I G_kGCm'C

Cuts at:      0    3896    4014 
   Size:       3896     118
 
BmgBI CAC'GTC

Cuts at:      0    3243    3897    4014 
   Size:       3243     654     117

  Fragments arranged by size: 

                3243     654     117
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      21     709    1345    2494    3863    4014 
   Size:         21     688     636    1149    1369     151

  Fragments arranged by size: 

                1369    1149     688     636     151      21
 
BmtI G_CTAG'C

Cuts at:      0    1559    4014 
   Size:       1559    2455
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1532    4014 
   Size:       1532    2482
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     816    1927    2290    4014 
   Size:        816    1111     363    1724

  Fragments arranged by size: 

                1724    1111     816     363
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    2746    3790    3837    4014 
   Size:        275    2471    1044      47     177

  Fragments arranged by size: 

                2471    1044     275     177      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3296    3410    4014 
   Size:       3296     114     604

  Fragments arranged by size: 

                3296     604     114
 
BsaAI yAC'GTr

Cuts at:      0    2561    4014 
   Size:       2561    1453
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     134     164    1970    2000    3022    3052    4014 
   Size:        134      30    1806      30    1022      30     962

  Fragments arranged by size: 

                1806    1022     962     134      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2962    4014 
   Size:       2962    1052
 
BseYI C'CCAG_C

Cuts at:      0     116    2682    4014 
   Size:        116    2566    1332

  Fragments arranged by size: 

                2566    1332     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2676    4014 
   Size:       2676    1338
 
BsiHKAI G_wGCw'C

Cuts at:      0     461    1844    2905    3363    4014 
   Size:        461    1383    1061     458     651

  Fragments arranged by size: 

                1383    1061     651     461     458
 
BsmI GAATG_Cn'

Cuts at:      0     994    3053    3705    4014 
   Size:        994    2059     652     309

  Fragments arranged by size: 

                2059     994     652     309
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1748    4014 
   Size:       1748    2266
 
Bsp1286I G_dGCh'C

Cuts at:      0     461    1844    2215    2905    2978    3363    3896    4014 
   Size:        461    1383     371     690      73     385     533     118

  Fragments arranged by size: 

                1383     690     533     461     385     371     118      73
            
 
BspHI T'CATG_A

Cuts at:      0    1868    3656    4014 
   Size:       1868    1788     358

  Fragments arranged by size: 

                1868    1788     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1014    1131    2687    3905    4014 
   Size:       1014     117    1556    1218     109

  Fragments arranged by size: 

                1556    1218    1014     117     109
 
BsrBI CCG'CTC

Cuts at:      0    2433    4014 
   Size:       2433    1581
 
BsrFI r'CCGG_y

Cuts at:      0    1058    4014 
   Size:       1058    2956
 
BsrGI T'GTAC_A

Cuts at:      0     413    3483    4014 
   Size:        413    3070     531

  Fragments arranged by size: 

                3070     531     413
 
BssHII G'CGCG_C

Cuts at:      0      35    4014 
   Size:         35    3979
 
BssSI C'ACGA_G

Cuts at:      0     777    1154    4014 
   Size:        777     377    2860

  Fragments arranged by size: 

                2860     777     377
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     126    4014 
   Size:        126    3888
 
BstEII G'GTnAC_C

Cuts at:      0    1118    4014 
   Size:       1118    2896
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2985    3027    4014 
   Size:       2985      42     987

  Fragments arranged by size: 

                2985     987      42
 
BstYI r'GATC_y

Cuts at:      0      64      90    2991    4014 
   Size:         64      26    2901    1023

  Fragments arranged by size: 

                2901    1023      64      26
 
BstZ17I GTA'TAC

Cuts at:      0    1923    4014 
   Size:       1923    2091
 
Bsu36I CC'TnA_GG

Cuts at:      0    3282    3337    4014 
   Size:       3282      55     677

  Fragments arranged by size: 

                3282     677      55
 
BtgI C'CryG_G

Cuts at:      0    2600    4014 
   Size:       2600    1414
 
BtsI GCAGTG_nn'

Cuts at:      0     142    1253    2187    2431    4014 
   Size:        142    1111     934     244    1583

  Fragments arranged by size: 

                1583    1111     934     244     142
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1248    1283    4014 
   Size:       1248      35    2731

  Fragments arranged by size: 

                2731    1248      35
 
DraI TTT'AAA

Cuts at:      0     107     506    1245    2085    4014 
   Size:        107     399     739     840    1929

  Fragments arranged by size: 

                1929     840     739     399     107
 
DraIII CAC_nnn'GTG

Cuts at:      0    2013    4014 
   Size:       2013    2001
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      22    4014 
   Size:         22    3992
 
EaeI y'GGCC_r

Cuts at:      0    1826    2015    2603    2655    3017    4014 
   Size:       1826     189     588      52     362     997

  Fragments arranged by size: 

                1826     997     588     362     189      52
 
EarI CTCTTCn'nnn_

Cuts at:      0    1184    1454    2162    2317    2496    2939    4014 
   Size:       1184     270     708     155     179     443    1075

  Fragments arranged by size: 

                1184    1075     708     443     270     179     155
 
EcoICRI GAG'CTC

Cuts at:      0     459    2903    3361    4014 
   Size:        459    2444     458     653

  Fragments arranged by size: 

                2444     653     459     458
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     816    1208    1927    2290    3622    3784    4014 
   Size:        467     349     392     719     363    1332     162     230

  Fragments arranged by size: 

                1332     719     467     392     363     349     230     162
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24    1713    2707    3063    4014 
   Size:         24    1689     994     356     951

  Fragments arranged by size: 

                1689     994     951     356      24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2375    2525    3831    4014 
   Size:       2375     150    1306     183

  Fragments arranged by size: 

                2375    1306     183     150
 
HaeII r_GCGC'y

Cuts at:      0    3771    4014 
   Size:       3771     243
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     132     164     642     674    2368    2400    3022    3054 
   Size:        132      32     478      32    1694      32     622      32

Cuts at:   3054    3232    3264    3345    3377    3967    3999    4014 
   Size:        178      32      81      32     590      32      15

  Fragments arranged by size: 

                1694     622     590     478     178     132      81      32
                  32      32      32      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0     966    1683    1737    2676    4014 
   Size:        966     717      54     939    1338

  Fragments arranged by size: 

                1338     966     939     717      54
 
HindIII A'AGCT_T

Cuts at:      0    1644    2892    4014 
   Size:       1644    1248    1122

  Fragments arranged by size: 

                1644    1248    1122
 
HpaI GTT'AAC

Cuts at:      0     966    1683    1737    2676    4014 
   Size:        966     717      54     939    1338

  Fragments arranged by size: 

                1338     966     939     717      54
 
MfeI C'AATT_G

Cuts at:      0     750    4014 
   Size:        750    3264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2777    4014 
   Size:       2777    1237
 
MscI TGG'CCA

Cuts at:      0    1828    2017    2657    3019    4014 
   Size:       1828     189     640     362     995

  Fragments arranged by size: 

                1828     995     640     362     189
 
MslI CAynn'nnrTG

Cuts at:      0     973     983    1003    1660    2051    2296    2559    2983 
   Size:        973      10      20     657     391     245     263     424

Cuts at:   2983    3352    3709    4014 
   Size:        369     357     305

  Fragments arranged by size: 

                 973     657     424     391     369     357     305     263
                 245      20      10
 
MspA1I CmG'CkG

Cuts at:      0      47     317    3887    4014 
   Size:         47     270    3570     127

  Fragments arranged by size: 

                3570     270     127      47
 
NdeI CA'TA_TG

Cuts at:      0    1368    4014 
   Size:       1368    2646
 
NheI G'CTAG_C

Cuts at:      0    1555    4014 
   Size:       1555    2459
 
NspI r_CATG'y

Cuts at:      0    2291    4014 
   Size:       2291    1723
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2456    4014 
   Size:       2456    1558
 
PfoI T'CCnGG_A

Cuts at:      0    1472    4014 
   Size:       1472    2542
 
PpuMI rG'GwC_Cy

Cuts at:      0      24    1713    3063    4014 
   Size:         24    1689    1350     951

  Fragments arranged by size: 

                1689    1350     951      24
 
PsiI TTA'TAA

Cuts at:      0    2392    3143    4014 
   Size:       2392     751     871

  Fragments arranged by size: 

                2392     871     751
 
PstI C_TGCA'G

Cuts at:      0     268    1909    4014 
   Size:        268    1641    2105

  Fragments arranged by size: 

                2105    1641     268
 
PvuII CAG'CTG

Cuts at:      0      47     317    3887    4014 
   Size:         47     270    3570     127

  Fragments arranged by size: 

                3570     270     127      47
 
SacI G_AGCT'C

Cuts at:      0     461    2905    3363    4014 
   Size:        461    2444     458     651

  Fragments arranged by size: 

                2444     651     461     458
 
ScaI AGT'ACT

Cuts at:      0     270    3370    4014 
   Size:        270    3100     644

  Fragments arranged by size: 

                3100     644     270
 
SfcI C'TryA_G

Cuts at:      0     264     312     876    1803    1905    3225    4014 
   Size:        264      48     564     927     102    1320     789

  Fragments arranged by size: 

                1320     927     789     564     264     102      48
 
SmaI CCC'GGG

Cuts at:      0    2470    3133    4014 
   Size:       2470     663     881

  Fragments arranged by size: 

                2470     881     663
 
SmlI C'TyrA_G

Cuts at:      0     290    2761    3805    3816    4014 
   Size:        290    2471    1044      11     198

  Fragments arranged by size: 

                2471    1044     290     198      11
 
StuI AGG'CCT

Cuts at:      0    2843    3799    4014 
   Size:       2843     956     215

  Fragments arranged by size: 

                2843     956     215
 
StyI C'CwwG_G

Cuts at:      0    1562    3794    4014 
   Size:       1562    2232     220

  Fragments arranged by size: 

                2232    1562     220
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130    4014 
   Size:        130    3884
 
TatI w'GTAC_w

Cuts at:      0     268     413     754    2612    3306    3368    3483    3995 
   Size:        268     145     341    1858     694      62     115     512

Cuts at:   3995    4014 
   Size:         19

  Fragments arranged by size: 

                1858     694     512     341     268     145     115      62
                  19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     134     166    1970    2002    2410    2442    4014 
   Size:        134      32    1804      32     408      32    1572

  Fragments arranged by size: 

                1804    1572     408     134      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1579    4014 
   Size:       1579    2435
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3886    4014 
   Size:       3886     128
 
XmaI C'CCGG_G

Cuts at:      0    2468    3131    4014 
   Size:       2468     663     883

  Fragments arranged by size: 

                2468     883     663
 
XmnI GAAnn'nnTTC

Cuts at:      0    3769    4014 
   Size:       3769     245
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AloI
    AlwNI     ApoI     AvaI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NheI     NspI    PflMI     PfoI    PpuMI     PsiI     PstI    PvuII
     SacI     ScaI     SfcI     SmaI     SmlI     StuI     StyI    TaqII
     TatI     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BfrBI     BglI   Bpu10I    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
    BspEI    BsrDI    BstBI    BtgZI     ClaI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI  Tth111I     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com