(Circular) (Six-base) MAPSORT of: pFN21AB9022.seq Check: 6149 from: 1 to: 9841 pFN21AB9022 9841 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7004 7004 Size: 9841 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7651 278 Size: 53 83 186 936 6115 2468 Fragments arranged by size: 6115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7184 1937 Size: 5247 4594 Acc65I G'GTAC_C Cuts at: 7162 7162 Size: 9841 AclI AA'CG_TT Cuts at: 1563 7968 8341 1563 Size: 6405 373 3063 Fragments arranged by size: 6405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4481 4895 5339 5375 5780 7904 9288 9556 Size: 2849 414 444 36 405 2124 1384 268 Cuts at: 9556 1632 Size: 1917 Fragments arranged by size: 2849 2124 1917 1384 444 414 405 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9644 AflIII A'CryG_T Cuts at: 1203 8740 9717 1203 Size: 7537 977 1327 Fragments arranged by size: 7537 1327 977 AgeI A'CCGG_T Cuts at: 8672 8672 Size: 9841 AhdI GACnn_n'nnGTC Cuts at: 6216 8570 6216 Size: 2354 7487 AleI CACnn'nnGTG Cuts at: 1166 3995 1166 Size: 2829 7012 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9841 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6677 6709 6677 Size: 32 9809 AlwNI CAG_nnn'CTG Cuts at: 4139 5642 5723 5912 6595 6771 7026 7116 7584 Size: 1503 81 189 683 176 255 90 468 Cuts at: 7584 9156 4139 Size: 1572 4824 Fragments arranged by size: 4824 1572 1503 683 468 255 189 176 90 81 ApaI G_GGCC'C Cuts at: 2207 2388 2451 2487 3039 3115 3275 3800 3863 Size: 181 63 36 552 76 160 525 63 Cuts at: 3863 3950 4059 4158 4302 4560 4929 5894 6003 Size: 87 109 99 144 258 369 965 109 Cuts at: 6003 6048 6267 6275 6321 2207 Size: 45 219 8 46 5727 Fragments arranged by size: 5727 965 552 525 369 258 219 181 160 144 109 109 99 87 76 63 63 46 45 36 8 ApaLI G'TGCA_C Cuts at: 6408 7898 9054 6408 Size: 1490 1156 7195 Fragments arranged by size: 7195 1490 1156 ApoI r'AATT_y Cuts at: 1493 2358 5296 7150 7376 7528 9637 1493 Size: 865 2938 1854 226 152 2109 1697 Fragments arranged by size: 2938 2109 1854 1697 865 226 152 AseI AT'TA_AT Cuts at: 160 1032 8395 160 Size: 872 7363 1606 Fragments arranged by size: 7363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9841 AvaI C'yCGr_G Cuts at: 1870 1943 2245 2371 2480 3003 3097 3151 3314 Size: 73 302 126 109 523 94 54 163 Cuts at: 3314 3707 4221 4346 4751 4823 6149 6167 6430 Size: 393 514 125 405 72 1326 18 263 Cuts at: 6430 7043 7166 1870 Size: 613 123 4545 Fragments arranged by size: 4545 1326 613 523 514 405 393 302 263 163 126 125 123 109 94 73 72 54 18 BamHI G'GATC_C Cuts at: 4226 4262 5586 5630 7171 7538 4226 Size: 36 1324 44 1541 367 6529 Fragments arranged by size: 6529 1541 1324 367 44 36 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2348 3083 4100 5222 5333 Size: 325 217 627 561 735 1017 1122 111 Cuts at: 5333 5612 5839 5857 6143 6247 6992 7162 8617 Size: 279 227 18 286 104 745 170 1455 Cuts at: 8617 618 Size: 1842 Fragments arranged by size: 1842 1455 1122 1017 745 735 627 561 325 286 279 227 217 170 111 104 18 BanII G_rGCy'C Cuts at: 1391 2207 2290 2388 2451 2487 2641 3039 3115 Size: 816 83 98 63 36 154 398 76 Cuts at: 3115 3275 3321 3415 3597 3750 3800 3863 3950 Size: 160 46 94 182 153 50 63 87 Cuts at: 3950 4059 4158 4302 4342 4543 4560 4929 5554 Size: 109 99 144 40 201 17 369 625 Cuts at: 5554 5761 5789 5894 5959 6003 6048 6166 6267 Size: 207 28 105 65 44 45 118 101 Cuts at: 6267 6275 6304 6321 6431 6510 6611 7064 7160 Size: 8 29 17 110 79 101 453 96 Cuts at: 7160 1391 Size: 4072 Fragments arranged by size: 4072 816 625 453 398 369 207 201 182 160 154 153 144 118 110 109 105 101 101 99 98 96 94 87 83 79 76 65 63 63 50 46 45 44 40 36 29 28 17 17 8 BbeI G_GCGC'C Cuts at: 5861 5861 Size: 9841 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3479 5314 928 Size: 971 1580 1835 5455 Fragments arranged by size: 5455 1835 1580 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8032 8066 1903 Size: 34 6095 34 3678 Fragments arranged by size: 6095 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5006 5042 6539 7733 8943 5006 Size: 36 1497 1194 1210 5904 Fragments arranged by size: 5904 1497 1210 1194 36 BclI T'GATC_A Cuts at: 1548 1548 Size: 9841 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2482 2979 3573 Size: 107 122 71 631 605 810 497 594 Cuts at: 3573 3771 3882 3891 3954 4251 4297 4473 4500 Size: 198 111 9 63 297 46 176 27 Cuts at: 4500 4564 4644 5002 5086 5247 6522 8452 136 Size: 64 80 358 84 161 1275 1930 1525 Fragments arranged by size: 1930 1525 1275 810 631 605 594 497 358 297 198 176 161 122 111 107 84 80 71 64 63 46 27 9 BglII A'GATC_T Cuts at: 9836 9836 Size: 9841 BlpI GC'TnA_GC Cuts at: 4341 7258 4341 Size: 2917 6924 Bme1580I G_kGCm'C Cuts at: 1790 2207 2388 2451 2487 3039 3115 3275 3708 Size: 417 181 63 36 552 76 160 433 Cuts at: 3708 3800 3863 3950 4059 4158 4302 4560 4929 Size: 92 63 87 109 99 144 258 369 Cuts at: 4929 5227 5336 5842 5894 6003 6048 6148 6250 Size: 298 109 506 52 109 45 100 102 Cuts at: 6250 6267 6275 6321 6412 6518 7902 9058 1790 Size: 17 8 46 91 106 1384 1156 2573 Fragments arranged by size: 2573 1384 1156 552 506 433 417 369 298 258 181 160 144 109 109 109 106 102 100 99 92 91 87 76 63 63 52 46 45 36 17 8 BmgBI CAC'GTC Cuts at: 1318 2183 2317 1318 Size: 865 134 8842 Fragments arranged by size: 8842 865 134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3881 5484 7572 Size: 416 53 83 399 24 2464 1603 2088 Cuts at: 7572 8530 442 Size: 958 1753 Fragments arranged by size: 2464 2088 1753 1603 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2583 2625 3288 3429 3786 3831 3930 4344 Size: 1344 42 663 141 357 45 99 414 Cuts at: 4344 4500 5442 5466 5745 5943 6288 6559 8501 Size: 156 942 24 279 198 345 271 1942 Cuts at: 8501 1239 Size: 2579 Fragments arranged by size: 2579 1942 1344 942 663 414 357 345 279 271 198 156 141 99 45 42 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2950 3581 7067 7966 8831 9129 2950 Size: 631 3486 899 865 298 3662 Fragments arranged by size: 3662 3486 899 865 631 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2007 2641 3021 3141 3237 3552 4661 Size: 617 508 634 380 120 96 315 1109 Cuts at: 4661 4813 5649 5742 6032 6120 8504 882 Size: 152 836 93 290 88 2384 2219 Fragments arranged by size: 2384 2219 1109 836 634 617 508 380 315 290 152 120 96 93 88 BsaAI yAC'GTr Cuts at: 493 1204 7632 493 Size: 711 6428 2702 Fragments arranged by size: 6428 2702 711 BsaBI GATnn'nnATC Cuts at: 2982 3192 9835 2982 Size: 210 6643 2988 Fragments arranged by size: 6643 2988 210 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3693 3915 5858 7648 Size: 53 83 186 936 2160 222 1943 1790 Cuts at: 7648 8030 8665 275 Size: 382 635 1451 Fragments arranged by size: 2160 1943 1790 1451 936 635 382 222 186 83 53 BsaWI w'CCGG_w Cuts at: 8274 8672 8946 9093 9504 8274 Size: 398 274 147 411 8611 Fragments arranged by size: 8611 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2461 2491 3561 3591 4615 Size: 30 165 30 1058 30 1070 30 1024 Cuts at: 4615 4645 5464 5494 6497 6527 1178 Size: 30 819 30 1003 30 4492 Fragments arranged by size: 4492 1070 1058 1024 1003 819 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 3214 4686 1186 Size: 1363 665 1472 6341 Fragments arranged by size: 6341 1472 1363 665 BseYI C'CCAG_C Cuts at: 2059 3443 5500 6314 6841 6845 9044 9540 2059 Size: 1384 2057 814 527 4 2199 496 2360 Fragments arranged by size: 2360 2199 2057 1384 814 527 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4257 1163 Size: 573 2521 6747 Fragments arranged by size: 6747 2521 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2602 7291 8052 9080 1540 Size: 275 186 601 4689 761 1028 2301 Fragments arranged by size: 4689 2301 1028 761 601 275 186 BsiHKAI G_wGCw'C Cuts at: 2641 3625 4342 4543 5554 5761 5959 6412 7902 Size: 984 717 201 1011 207 198 453 1490 Cuts at: 7902 7987 9058 2641 Size: 85 1071 3424 Fragments arranged by size: 3424 1490 1071 1011 984 717 453 207 201 198 85 BsmI GAATG_Cn' Cuts at: 3455 4743 5046 6980 7352 7445 3455 Size: 1288 303 1934 372 93 5851 Fragments arranged by size: 5851 1934 1288 372 303 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3906 4167 6894 3906 Size: 261 2727 6853 Fragments arranged by size: 6853 2727 261 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2290 2388 2451 2487 2641 3039 Size: 399 417 83 98 63 36 154 398 Cuts at: 3039 3115 3275 3321 3415 3597 3625 3708 3750 Size: 76 160 46 94 182 28 83 42 Cuts at: 3750 3800 3863 3950 4059 4158 4302 4342 4543 Size: 50 63 87 109 99 144 40 201 Cuts at: 4543 4560 4929 5227 5336 5554 5761 5789 5842 Size: 17 369 298 109 218 207 28 53 Cuts at: 5842 5894 5959 6003 6048 6148 6166 6250 6267 Size: 52 65 44 45 100 18 84 17 Cuts at: 6267 6275 6304 6321 6412 6431 6510 6518 6611 Size: 8 29 17 91 19 79 8 93 Cuts at: 6611 7064 7160 7902 7987 9058 1391 Size: 453 96 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 417 399 398 369 298 218 207 201 182 160 154 144 109 109 100 99 98 96 94 93 91 87 85 84 83 83 79 76 65 63 63 53 52 50 46 45 44 42 40 36 29 28 28 19 18 17 17 17 8 8 BspEI T'CCGG_A Cuts at: 9504 9504 Size: 9841 BspHI T'CATG_A Cuts at: 7730 9460 7730 Size: 1730 8111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3058 4169 7004 7196 844 Size: 2214 1111 2835 192 3489 Fragments arranged by size: 3489 2835 2214 1111 192 BsrBI CCG'CTC Cuts at: 1394 1823 3679 5329 7728 1394 Size: 429 1856 1650 2399 3507 Fragments arranged by size: 3507 2399 1856 1650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6985 8336 8510 65 Size: 1183 5737 1351 174 1396 Fragments arranged by size: 5737 1396 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 3917 7088 8485 8672 1700 Size: 110 220 1887 3171 1397 187 2869 Fragments arranged by size: 3171 2869 1887 1397 220 187 110 BsrGI T'GTAC_A Cuts at: 96 3458 96 Size: 3362 6479 BssHII G'CGCG_C Cuts at: 1925 2020 1925 Size: 95 9746 BssSI C'ACGA_G Cuts at: 7901 8913 7901 Size: 1012 8829 BstAPI GCAn_nnn'nTGC Cuts at: 794 3083 794 Size: 2289 7552 BstBI TT'CG_AA Cuts at: 8645 8645 Size: 9841 BstEII G'GTnAC_C Cuts at: 1184 2918 3092 5690 1184 Size: 1734 174 2598 5335 Fragments arranged by size: 5335 2598 1734 174 BstXI CCAn_nnnn'nTGG Cuts at: 3429 6849 3429 Size: 3420 6421 BstYI r'GATC_y Cuts at: 1972 4226 4262 5586 5630 7171 7538 7923 7940 Size: 2254 36 1324 44 1541 367 385 17 Cuts at: 7940 9392 9478 9490 9836 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2254 1977 1541 1452 1324 385 367 346 86 44 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 3415 4352 5581 5590 5650 Size: 552 939 69 1342 937 1229 9 60 Cuts at: 5650 6735 6988 513 Size: 1085 253 3366 Fragments arranged by size: 3366 1342 1229 1085 939 937 552 253 69 60 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2773 3756 6781 6787 487 Size: 683 56 1547 983 3025 6 3541 Fragments arranged by size: 3541 3025 1547 983 683 56 6 BtsI GCAGTG_nn' Cuts at: 2152 2522 2848 4245 6099 6711 6835 7357 8151 Size: 370 326 1397 1854 612 124 522 794 Cuts at: 8151 8171 2152 Size: 20 3822 Fragments arranged by size: 3822 1854 1397 794 612 522 370 326 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9806 DraI TTT'AAA Cuts at: 4723 4903 7145 4723 Size: 180 2242 7419 Fragments arranged by size: 7419 2242 180 DraIII CAC_nnn'GTG Cuts at: 5846 5846 Size: 9841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2625 5123 8848 809 Size: 507 1309 2498 3725 1802 Fragments arranged by size: 3725 2498 1802 1309 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2599 3565 Size: 54 1425 50 275 18 198 571 966 Cuts at: 3565 4492 4546 5092 5543 6397 6738 6792 6913 Size: 927 54 546 451 854 341 54 121 Cuts at: 6913 7288 8177 9806 8 Size: 375 889 1629 43 Fragments arranged by size: 1629 1425 966 927 889 854 571 546 451 375 341 275 198 121 54 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2599 7288 1537 Size: 275 787 4689 4090 Fragments arranged by size: 4689 4090 787 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 3370 5925 6322 7771 1353 Size: 778 1239 2555 397 1449 3423 Fragments arranged by size: 3423 2555 1449 1239 778 397 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5521 6868 7060 8428 8802 8948 Size: 1064 138 3932 1347 192 1368 374 146 Cuts at: 8948 387 Size: 1280 Fragments arranged by size: 3932 1368 1347 1280 1064 374 192 146 138 EcoICRI GAG'CTC Cuts at: 2639 4340 4541 5552 5759 5957 2639 Size: 1701 201 1011 207 198 6523 Fragments arranged by size: 6523 1701 1011 207 201 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2583 2625 3288 3429 3786 3831 3930 Size: 393 951 42 663 141 357 45 99 Cuts at: 3930 4344 4481 4500 4895 5339 5375 5442 5466 Size: 414 137 19 395 444 36 67 24 Cuts at: 5466 5745 5780 5943 6288 6559 7904 8501 9288 Size: 279 35 163 345 271 1345 597 787 Cuts at: 9288 9556 1239 Size: 268 1524 Fragments arranged by size: 1524 1345 951 787 663 597 444 414 395 393 357 345 279 271 268 163 141 137 99 67 45 42 36 35 24 19 EcoNI CCTnn'n_nnAGG Cuts at: 2172 2172 Size: 9841 EcoO109I rG'GnC_Cy Cuts at: 2204 2303 2447 2448 2484 2591 2750 2990 3008 Size: 99 144 1 36 107 159 240 18 Cuts at: 3008 3035 3036 3111 3164 3224 3271 3299 3308 Size: 27 1 75 53 60 47 28 9 Cuts at: 3308 3503 3773 3796 3797 3808 3860 3947 3966 Size: 195 270 23 1 11 52 87 19 Cuts at: 3966 4055 4082 4091 4154 4199 4217 4271 4299 Size: 89 27 9 63 45 18 54 28 Cuts at: 4299 4315 4412 4484 4556 4557 4612 4746 4918 Size: 16 97 72 72 1 55 134 172 Cuts at: 4918 4926 4994 5432 5672 5720 5890 6000 6044 Size: 8 68 438 240 48 170 110 44 Cuts at: 6044 6107 6116 6218 6236 6263 6264 6271 6272 Size: 63 9 102 18 27 1 7 1 Cuts at: 6272 6318 6371 6592 6787 2204 Size: 46 53 221 195 5258 Fragments arranged by size: 5258 438 270 240 240 221 195 195 172 170 159 144 134 110 107 102 99 97 89 87 75 72 72 68 63 63 60 55 54 53 53 52 48 47 46 45 44 36 28 28 27 27 27 23 19 18 18 18 16 11 9 9 9 8 7 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 2358 7150 7528 2358 Size: 4792 378 4671 Fragments arranged by size: 4792 4671 378 EcoRV GAT'ATC Cuts at: 2549 2549 Size: 9841 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5380 7039 5380 Size: 1659 8182 FseI GG_CCGG'CC Cuts at: 2034 7092 2034 Size: 5058 4783 FspI TGC'GCA Cuts at: 8347 8347 Size: 9841 HaeII r_GCGC'y Cuts at: 1678 4632 5861 8988 1678 Size: 2954 1229 3127 2531 Fragments arranged by size: 3127 2954 2531 1229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4757 4789 5464 5496 6202 Size: 32 135 32 3185 32 675 32 706 Cuts at: 6202 6234 8482 8514 8556 8588 1373 Size: 32 2248 32 42 32 2626 Fragments arranged by size: 3185 2626 2248 706 675 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7185 7431 669 Size: 988 281 5247 246 3079 Fragments arranged by size: 5247 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9841 HpaI GTT'AAC Cuts at: 7431 7431 Size: 9841 KasI G'GCGC_C Cuts at: 5857 5857 Size: 9841 KpnI G_GTAC'C Cuts at: 7166 7166 Size: 9841 MfeI C'AATT_G Cuts at: 7440 7440 Size: 9841 MluI A'CGCG_T Cuts at: 9717 9717 Size: 9841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2614 3991 5850 6226 8955 9139 1305 Size: 1309 1377 1859 376 2729 184 2007 Fragments arranged by size: 2729 2007 1859 1377 1309 376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4548 6399 6915 10 Size: 54 1425 343 2716 1851 516 2936 Fragments arranged by size: 2936 2716 1851 1425 516 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3995 7799 8158 8317 518 Size: 648 2829 3804 359 159 2042 Fragments arranged by size: 3804 2829 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3417 5592 6721 6771 6845 6939 6990 7116 Size: 1811 2175 1129 50 74 94 51 126 Cuts at: 7116 7257 7934 9082 9327 1606 Size: 141 677 1148 245 2120 Fragments arranged by size: 2175 2120 1811 1148 1129 677 245 141 126 94 74 51 50 NaeI GCC'GGC Cuts at: 2032 3919 7090 2032 Size: 1887 3171 4783 Fragments arranged by size: 4783 3171 1887 NarI GG'CG_CC Cuts at: 5858 5858 Size: 9841 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 8350 Fragments arranged by size: 8350 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9841 NgoMIV G'CCGG_C Cuts at: 2030 3917 7088 2030 Size: 1887 3171 4783 Fragments arranged by size: 4783 3171 1887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9841 NotI GC'GGCC_GC Cuts at: 7288 7288 Size: 9841 NspI r_CATG'y Cuts at: 1132 1229 2615 4804 7199 8744 1132 Size: 97 1386 2189 2395 1545 2229 Fragments arranged by size: 2395 2229 2189 1545 1386 97 PasI CC'CwG_GG Cuts at: 1348 2209 2388 2425 2606 3032 3107 3389 3581 Size: 861 179 37 181 426 75 282 192 Cuts at: 3581 3590 4158 4205 4304 4616 4922 5054 5072 Size: 9 568 47 99 312 306 132 18 Cuts at: 5072 5156 5228 5402 5969 6004 6048 6223 6242 Size: 84 72 174 567 35 44 175 19 Cuts at: 6242 6268 1348 Size: 26 4921 Fragments arranged by size: 4921 861 568 567 426 312 306 282 192 181 179 175 174 132 99 84 75 72 47 44 37 35 26 19 18 9 PciI A'CATG_T Cuts at: 8740 8740 Size: 9841 PflMI CCAn_nnn'nTGG Cuts at: 1108 5982 6595 6922 1108 Size: 4874 613 327 4027 Fragments arranged by size: 4874 4027 613 327 PfoI T'CCnGG_A Cuts at: 2362 2905 3304 3490 3625 3820 3847 4399 4516 Size: 543 399 186 135 195 27 552 117 Cuts at: 4516 4705 5437 5572 5626 5923 5959 2362 Size: 189 732 135 54 297 36 6244 Fragments arranged by size: 6244 732 552 543 399 297 195 189 186 135 135 117 54 36 27 PmeI GTTT'AAAC Cuts at: 7145 7145 Size: 9841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7910 7942 9423 9455 7910 Size: 32 1481 32 8296 Fragments arranged by size: 8296 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2303 2591 2750 3008 3164 3224 3308 3503 3808 Size: 288 159 258 156 60 84 195 305 Cuts at: 3808 3966 4082 4199 4217 4271 4484 4612 4746 Size: 158 116 117 18 54 213 128 134 Cuts at: 4746 4918 6116 6218 6371 6592 2303 Size: 172 1198 102 153 221 5552 Fragments arranged by size: 5552 1198 305 288 258 221 213 195 172 159 158 156 153 134 128 117 116 102 84 60 54 18 PshAI GACnn'nnGTC Cuts at: 1613 2436 4179 5423 1613 Size: 823 1743 1244 6031 Fragments arranged by size: 6031 1743 1244 823 PsiI TTA'TAA Cuts at: 7411 7411 Size: 9841 PspOMI G'GGCC_C Cuts at: 2203 2384 2447 2483 3035 3111 3271 3796 3859 Size: 181 63 36 552 76 160 525 63 Cuts at: 3859 3946 4055 4154 4298 4556 4925 5890 5999 Size: 87 109 99 144 258 369 965 109 Cuts at: 5999 6044 6263 6271 6317 2203 Size: 45 219 8 46 5727 Fragments arranged by size: 5727 965 552 525 369 258 219 181 160 144 109 109 99 87 76 63 63 46 45 36 8 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3826 3858 3826 Size: 32 9809 PstI C_TGCA'G Cuts at: 830 2256 7193 830 Size: 1426 4937 3478 Fragments arranged by size: 4937 3478 1426 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9841 PvuII CAG'CTG Cuts at: 6845 6939 6845 Size: 94 9747 SacI G_AGCT'C Cuts at: 2641 4342 4543 5554 5761 5959 2641 Size: 1701 201 1011 207 198 6523 Fragments arranged by size: 6523 1701 1011 207 201 198 SacII CC_GC'GG Cuts at: 3418 5593 6991 3418 Size: 2175 1398 6268 Fragments arranged by size: 6268 2175 1398 SalI G'TCGA_C Cuts at: 1936 7183 1936 Size: 5247 4594 SanDI GG'GwC_CC Cuts at: 2750 3008 3224 3808 3966 4612 4746 4918 6116 Size: 258 216 584 158 646 134 172 1198 Cuts at: 6116 6218 2750 Size: 102 6373 Fragments arranged by size: 6373 1198 646 584 258 216 172 158 134 102 SapI GCTCTTCn'nnn_ Cuts at: 3370 5925 3370 Size: 2555 7286 SbfI CC_TGCA'GG Cuts at: 7193 7193 Size: 9841 ScaI AGT'ACT Cuts at: 8089 9801 8089 Size: 1712 8129 SexAI A'CCwGG_T Cuts at: 5347 5563 5842 5905 6286 7137 5347 Size: 216 279 63 381 851 8051 Fragments arranged by size: 8051 851 381 279 216 63 SfcI C'TryA_G Cuts at: 826 1045 2252 7189 8324 9005 9196 826 Size: 219 1207 4937 1135 681 191 1471 Fragments arranged by size: 4937 1471 1207 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2482 3573 3771 3882 3891 3954 4251 4297 4473 Size: 1091 198 111 9 63 297 46 176 Cuts at: 4473 4500 2482 Size: 27 7823 Fragments arranged by size: 7823 1091 297 198 176 111 63 46 27 9 SfoI GGC'GCC Cuts at: 5859 5859 Size: 9841 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9841 SmaI CCC'GGG Cuts at: 1872 2247 2373 2482 3005 3099 3153 3316 3709 Size: 375 126 109 523 94 54 163 393 Cuts at: 3709 4348 4753 4825 6151 6169 6432 7168 1872 Size: 639 405 72 1326 18 263 736 4545 Fragments arranged by size: 4545 1326 736 639 523 405 393 375 263 163 126 109 94 72 54 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 2929 3596 7082 7945 8846 9108 Size: 197 926 986 667 3486 863 901 262 Cuts at: 9108 820 Size: 1553 Fragments arranged by size: 3486 1553 986 926 901 863 667 262 197 SnaBI TAC'GTA Cuts at: 493 7632 493 Size: 7139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9841 SphI G_CATG'C Cuts at: 1132 4804 7199 1132 Size: 3672 2395 3774 Fragments arranged by size: 3774 3672 2395 SrfI GCCC'GGGC Cuts at: 4348 6432 4348 Size: 2084 7757 SspI AAT'ATT Cuts at: 5 52 7765 5 Size: 47 7713 2081 Fragments arranged by size: 7713 2081 47 StuI AGG'CCT Cuts at: 1517 2973 3207 4074 4110 4395 4476 4503 5241 Size: 1456 234 867 36 285 81 27 738 Cuts at: 5241 5316 6013 1517 Size: 75 697 5345 Fragments arranged by size: 5345 1456 867 738 697 285 234 81 75 36 27 StyI C'CwwG_G Cuts at: 513 1065 2004 2307 2488 3012 3115 3228 3507 Size: 552 939 303 181 524 103 113 279 Cuts at: 3507 4059 4435 4560 4795 5035 5428 5616 5752 Size: 552 376 125 235 240 393 188 136 Cuts at: 5752 6111 6120 6322 7280 513 Size: 359 9 202 958 3074 Fragments arranged by size: 3074 958 939 552 552 524 393 376 359 303 279 240 235 202 188 181 136 125 113 103 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2829 6837 7868 7885 8038 8223 8668 Size: 1255 1249 4008 1031 17 153 185 445 Cuts at: 8668 325 Size: 1498 Fragments arranged by size: 4008 1498 1255 1249 1031 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3458 6558 Size: 274 80 33 51 1443 846 635 3100 Cuts at: 6558 6645 7017 8087 9799 96 Size: 87 372 1070 1712 138 Fragments arranged by size: 3100 1712 1443 1070 846 635 372 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2461 2493 3703 3735 3924 3956 4615 4647 6497 Size: 32 1210 32 189 32 659 32 1850 Cuts at: 6497 6529 9801 9833 2461 Size: 32 3272 32 2469 Fragments arranged by size: 3272 2469 1850 1210 659 189 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2184 1316 Size: 868 8973 XbaI T'CTAG_A Cuts at: 6943 7177 6943 Size: 234 9607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2216 2314 4237 4442 4802 5435 6011 6091 6329 Size: 98 1923 205 360 633 576 80 238 Cuts at: 6329 6392 6517 6733 2216 Size: 63 125 216 5324 Fragments arranged by size: 5324 1923 633 576 360 238 216 205 125 98 80 63 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9841 XmaI C'CCGG_G Cuts at: 1870 2245 2371 2480 3003 3097 3151 3314 3707 Size: 375 126 109 523 94 54 163 393 Cuts at: 3707 4346 4751 4823 6149 6167 6430 7166 1870 Size: 639 405 72 1326 18 263 736 4545 Fragments arranged by size: 4545 1326 736 639 523 405 393 375 263 163 126 109 94 72 54 18 XmnI GAAnn'nnTTC Cuts at: 5360 7970 5360 Size: 2610 7231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7649 276 Size: 53 83 186 936 6115 2468 Fragments arranged by size: 6115 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I FspAI NruI NsiI PacI PmlI PspXI RsrII SwaI