Restriction map for
pFN21AB9022
(Linear) (Six-base) MAPSORT of: KIBB9022.seq Check: 3040 from: 1 to: 5136
KIBB9022.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4999 5136
Size: 4999 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2476 2890 3334 3370 3775 5136
Size: 2476 414 444 36 405 1361
Fragments arranged by size:
2476 1361 444 414 405 36
AhdI GACnn_n'nnGTC
Cuts at: 0 4211 5136
Size: 4211 925
AleI CACnn'nnGTG
Cuts at: 0 1990 5136
Size: 1990 3146
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4672 4704 5136
Size: 4672 32 432
Fragments arranged by size:
4672 432 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2134 3637 3718 3907 4590 4766 5021 5111
Size: 2134 1503 81 189 683 176 255 90
Cuts at: 5111 5136
Size: 25
Fragments arranged by size:
2134 1503 683 255 189 176 90 81
25
ApaI G_GGCC'C
Cuts at: 0 202 383 446 482 1034 1110 1270 1795
Size: 202 181 63 36 552 76 160 525
Cuts at: 1795 1858 1945 2054 2153 2297 2555 2924 3889
Size: 63 87 109 99 144 258 369 965
Cuts at: 3889 3998 4043 4262 4270 4316 5136
Size: 109 45 219 8 46 820
Fragments arranged by size:
965 820 552 525 369 258 219 202
181 160 144 109 109 99 87 76
63 63 46 45 36 8
ApaLI G'TGCA_C
Cuts at: 0 4403 5136
Size: 4403 733
ApoI r'AATT_y
Cuts at: 0 353 3291 5136
Size: 353 2938 1845
Fragments arranged by size:
2938 1845 353
AvaI C'yCGr_G
Cuts at: 0 240 366 475 998 1092 1146 1309 1702
Size: 240 126 109 523 94 54 163 393
Cuts at: 1702 2216 2341 2746 2818 4144 4162 4425 5038
Size: 514 125 405 72 1326 18 263 613
Cuts at: 5038 5136
Size: 98
Fragments arranged by size:
1326 613 523 514 405 393 263 240
163 126 125 109 98 94 72 54
18
BamHI G'GATC_C
Cuts at: 0 2221 2257 3581 3625 5136
Size: 2221 36 1324 44 1511
Fragments arranged by size:
2221 1511 1324 44 36
BanI G'GyrC_C
Cuts at: 0 343 1078 2095 3217 3328 3607 3834 3852
Size: 343 735 1017 1122 111 279 227 18
Cuts at: 3852 4138 4242 4987 5136
Size: 286 104 745 149
Fragments arranged by size:
1122 1017 745 735 343 286 279 227
149 111 104 18
BanII G_rGCy'C
Cuts at: 0 202 285 383 446 482 636 1034 1110
Size: 202 83 98 63 36 154 398 76
Cuts at: 1110 1270 1316 1410 1592 1745 1795 1858 1945
Size: 160 46 94 182 153 50 63 87
Cuts at: 1945 2054 2153 2297 2337 2538 2555 2924 3549
Size: 109 99 144 40 201 17 369 625
Cuts at: 3549 3756 3784 3889 3954 3998 4043 4161 4262
Size: 207 28 105 65 44 45 118 101
Cuts at: 4262 4270 4299 4316 4426 4505 4606 5059 5136
Size: 8 29 17 110 79 101 453 77
Fragments arranged by size:
625 453 398 369 207 202 201 182
160 154 153 144 118 110 109 105
101 101 99 98 94 87 83 79
77 76 65 63 63 50 46 45
44 40 36 29 28 17 17 8
BbeI G_GCGC'C
Cuts at: 0 3856 5136
Size: 3856 1280
BbsI GAAGACnn'nnnn_
Cuts at: 0 1474 3309 5136
Size: 1474 1835 1827
Fragments arranged by size:
1835 1827 1474
BciVI GTATCCnnnnn_n'
Cuts at: 0 3001 3037 4534 5136
Size: 3001 36 1497 602
Fragments arranged by size:
3001 1497 602 36
BglI GCCn_nnn'nGGC
Cuts at: 0 477 974 1568 1766 1877 1886 1949 2246
Size: 477 497 594 198 111 9 63 297
Cuts at: 2246 2292 2468 2495 2559 2639 2997 3081 3242
Size: 46 176 27 64 80 358 84 161
Cuts at: 3242 4517 5136
Size: 1275 619
Fragments arranged by size:
1275 619 594 497 477 358 297 198
176 161 111 84 80 64 63 46
27 9
BlpI GC'TnA_GC
Cuts at: 0 2336 5136
Size: 2336 2800
Bme1580I G_kGCm'C
Cuts at: 0 202 383 446 482 1034 1110 1270 1703
Size: 202 181 63 36 552 76 160 433
Cuts at: 1703 1795 1858 1945 2054 2153 2297 2555 2924
Size: 92 63 87 109 99 144 258 369
Cuts at: 2924 3222 3331 3837 3889 3998 4043 4143 4245
Size: 298 109 506 52 109 45 100 102
Cuts at: 4245 4262 4270 4316 4407 4513 5136
Size: 17 8 46 91 106 623
Fragments arranged by size:
623 552 506 433 369 298 258 202
181 160 144 109 109 109 106 102
100 99 92 91 87 76 63 63
52 46 45 36 17 8
BmgBI CAC'GTC
Cuts at: 0 178 312 5136
Size: 178 134 4824
Fragments arranged by size:
4824 178 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 1876 3479 5136
Size: 1876 1603 1657
Fragments arranged by size:
1876 1657 1603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 620 1283 1424 1781 1826 1925 2339
Size: 578 42 663 141 357 45 99 414
Cuts at: 2339 2495 3437 3461 3740 3938 4283 4554 5136
Size: 156 942 24 279 198 345 271 582
Fragments arranged by size:
942 663 582 578 414 357 345 279
271 198 156 141 99 45 42 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1576 5062 5136
Size: 945 631 3486 74
Fragments arranged by size:
3486 945 631 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 2 636 1016 1136 1232 1547 2656 2808
Size: 2 634 380 120 96 315 1109 152
Cuts at: 2808 3644 3737 4027 4115 5136
Size: 836 93 290 88 1021
Fragments arranged by size:
1109 1021 836 634 380 315 290 152
120 96 93 88 2
BsaBI GATnn'nnATC
Cuts at: 0 977 1187 5136
Size: 977 210 3949
Fragments arranged by size:
3949 977 210
BsaHI Gr'CG_yC
Cuts at: 0 1688 1910 3853 5136
Size: 1688 222 1943 1283
Fragments arranged by size:
1943 1688 1283 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 456 486 1556 1586 2610 2640 3459 3489
Size: 456 30 1070 30 1024 30 819 30
Cuts at: 3489 4492 4522 5136
Size: 1003 30 614
Fragments arranged by size:
1070 1024 1003 819 614 456 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 1209 2681 5136
Size: 544 665 1472 2455
Fragments arranged by size:
2455 1472 665 544
BseYI C'CCAG_C
Cuts at: 0 54 1438 3495 4309 4836 4840 5136
Size: 54 1384 2057 814 527 4 296
Fragments arranged by size:
2057 1384 814 527 296 54 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2252 5136
Size: 2252 2884
BsiEI CG_ry'CG
Cuts at: 0 597 5136
Size: 597 4539
BsiHKAI G_wGCw'C
Cuts at: 0 636 1620 2337 2538 3549 3756 3954 4407
Size: 636 984 717 201 1011 207 198 453
Cuts at: 4407 5136
Size: 729
Fragments arranged by size:
1011 984 729 717 636 453 207 201
198
BsmI GAATG_Cn'
Cuts at: 0 1450 2738 3041 4975 5136
Size: 1450 1288 303 1934 161
Fragments arranged by size:
1934 1450 1288 303 161
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1901 2162 4889 5136
Size: 1901 261 2727 247
Fragments arranged by size:
2727 1901 261 247
Bsp1286I G_dGCh'C
Cuts at: 0 202 285 383 446 482 636 1034 1110
Size: 202 83 98 63 36 154 398 76
Cuts at: 1110 1270 1316 1410 1592 1620 1703 1745 1795
Size: 160 46 94 182 28 83 42 50
Cuts at: 1795 1858 1945 2054 2153 2297 2337 2538 2555
Size: 63 87 109 99 144 40 201 17
Cuts at: 2555 2924 3222 3331 3549 3756 3784 3837 3889
Size: 369 298 109 218 207 28 53 52
Cuts at: 3889 3954 3998 4043 4143 4161 4245 4262 4270
Size: 65 44 45 100 18 84 17 8
Cuts at: 4270 4299 4316 4407 4426 4505 4513 4606 5059
Size: 29 17 91 19 79 8 93 453
Cuts at: 5059 5136
Size: 77
Fragments arranged by size:
453 398 369 298 218 207 202 201
182 160 154 144 109 109 100 99
98 94 93 91 87 84 83 83
79 77 76 65 63 63 53 52
50 46 45 44 42 40 36 29
28 28 19 18 17 17 17 8
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1053 2164 4999 5136
Size: 1053 1111 2835 137
Fragments arranged by size:
2835 1111 1053 137
BsrBI CCG'CTC
Cuts at: 0 1674 3324 5136
Size: 1674 1650 1812
Fragments arranged by size:
1812 1674 1650
BsrDI GCAATG_nn'
Cuts at: 0 4980 5136
Size: 4980 156
BsrFI r'CCGG_y
Cuts at: 0 25 1912 5083 5136
Size: 25 1887 3171 53
Fragments arranged by size:
3171 1887 53 25
BsrGI T'GTAC_A
Cuts at: 0 1453 5136
Size: 1453 3683
BssHII G'CGCG_C
Cuts at: 0 15 5136
Size: 15 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1078 5136
Size: 1078 4058
BstEII G'GTnAC_C
Cuts at: 0 913 1087 3685 5136
Size: 913 174 2598 1451
Fragments arranged by size:
2598 1451 913 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1424 4844 5136
Size: 1424 3420 292
Fragments arranged by size:
3420 1424 292
BstYI r'GATC_y
Cuts at: 0 2221 2257 3581 3625 5136
Size: 2221 36 1324 44 1511
Fragments arranged by size:
2221 1511 1324 44 36
BtgI C'CryG_G
Cuts at: 0 68 1410 2347 3576 3585 3645 4730 4983
Size: 68 1342 937 1229 9 60 1085 253
Cuts at: 4983 5136
Size: 153
Fragments arranged by size:
1342 1229 1085 937 253 153 68 60
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 768 1751 4776 4782 5136
Size: 768 983 3025 6 354
Fragments arranged by size:
3025 983 768 354 6
BtsI GCAGTG_nn'
Cuts at: 0 147 517 843 2240 4094 4706 4830 5136
Size: 147 370 326 1397 1854 612 124 306
Fragments arranged by size:
1854 1397 612 370 326 306 147 124
DraI TTT'AAA
Cuts at: 0 2718 2898 5136
Size: 2718 180 2238
Fragments arranged by size:
2718 2238 180
DraIII CAC_nnn'GTG
Cuts at: 0 3841 5136
Size: 3841 1295
DrdI GACnn_nn'nnGTC
Cuts at: 0 620 3118 5136
Size: 620 2498 2018
Fragments arranged by size:
2498 2018 620
EaeI y'GGCC_r
Cuts at: 0 23 594 1560 2487 2541 3087 3538 4392
Size: 23 571 966 927 54 546 451 854
Cuts at: 4392 4733 4787 4908 5136
Size: 341 54 121 228
Fragments arranged by size:
966 927 854 571 546 451 341 228
121 54 54 23
EagI C'GGCC_G
Cuts at: 0 594 5136
Size: 594 4542
EarI CTCTTCn'nnn_
Cuts at: 0 126 1365 3920 4317 5136
Size: 126 1239 2555 397 819
Fragments arranged by size:
2555 1239 819 397 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3516 4863 5055 5136
Size: 3516 1347 192 81
Fragments arranged by size:
3516 1347 192 81
EcoICRI GAG'CTC
Cuts at: 0 634 2335 2536 3547 3754 3952 5136
Size: 634 1701 201 1011 207 198 1184
Fragments arranged by size:
1701 1184 1011 634 207 201 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 620 1283 1424 1781 1826 1925 2339
Size: 578 42 663 141 357 45 99 414
Cuts at: 2339 2476 2495 2890 3334 3370 3437 3461 3740
Size: 137 19 395 444 36 67 24 279
Cuts at: 3740 3775 3938 4283 4554 5136
Size: 35 163 345 271 582
Fragments arranged by size:
663 582 578 444 414 395 357 345
279 271 163 141 137 99 67 45
42 36 35 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 167 5136
Size: 167 4969
EcoO109I rG'GnC_Cy
Cuts at: 0 199 298 442 443 479 586 745 985
Size: 199 99 144 1 36 107 159 240
Cuts at: 985 1003 1030 1031 1106 1159 1219 1266 1294
Size: 18 27 1 75 53 60 47 28
Cuts at: 1294 1303 1498 1768 1791 1792 1803 1855 1942
Size: 9 195 270 23 1 11 52 87
Cuts at: 1942 1961 2050 2077 2086 2149 2194 2212 2266
Size: 19 89 27 9 63 45 18 54
Cuts at: 2266 2294 2310 2407 2479 2551 2552 2607 2741
Size: 28 16 97 72 72 1 55 134
Cuts at: 2741 2913 2921 2989 3427 3667 3715 3885 3995
Size: 172 8 68 438 240 48 170 110
Cuts at: 3995 4039 4102 4111 4213 4231 4258 4259 4266
Size: 44 63 9 102 18 27 1 7
Cuts at: 4266 4267 4313 4366 4587 4782 5136
Size: 1 46 53 221 195 354
Fragments arranged by size:
438 354 270 240 240 221 199 195
195 172 170 159 144 134 110 107
102 99 97 89 87 75 72 72
68 63 63 60 55 54 53 53
52 48 47 46 45 44 36 28
28 27 27 27 23 19 18 18
18 16 11 9 9 9 8 7
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 353 5136
Size: 353 4783
EcoRV GAT'ATC
Cuts at: 0 544 5136
Size: 544 4592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3375 5034 5136
Size: 3375 1659 102
Fragments arranged by size:
3375 1659 102
FseI GG_CCGG'CC
Cuts at: 0 29 5087 5136
Size: 29 5058 49
Fragments arranged by size:
5058 49 29
HaeII r_GCGC'y
Cuts at: 0 2627 3856 5136
Size: 2627 1229 1280
Fragments arranged by size:
2627 1280 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2752 2784 3459 3491 4197 4229 5136
Size: 2752 32 675 32 706 32 907
Fragments arranged by size:
2752 907 706 675 32 32 32
KasI G'GCGC_C
Cuts at: 0 3852 5136
Size: 3852 1284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1986 3845 4221 5136
Size: 609 1377 1859 376 915
Fragments arranged by size:
1859 1377 915 609 376
MscI TGG'CCA
Cuts at: 0 2543 4394 4910 5136
Size: 2543 1851 516 226
Fragments arranged by size:
2543 1851 516 226
MslI CAynn'nnrTG
Cuts at: 0 1990 5136
Size: 1990 3146
MspA1I CmG'CkG
Cuts at: 0 1412 3587 4716 4766 4840 4934 4985 5111
Size: 1412 2175 1129 50 74 94 51 126
Cuts at: 5111 5136
Size: 25
Fragments arranged by size:
2175 1412 1129 126 94 74 51 50
25
NaeI GCC'GGC
Cuts at: 0 27 1914 5085 5136
Size: 27 1887 3171 51
Fragments arranged by size:
3171 1887 51 27
NarI GG'CG_CC
Cuts at: 0 3853 5136
Size: 3853 1283
NgoMIV G'CCGG_C
Cuts at: 0 25 1912 5083 5136
Size: 25 1887 3171 53
Fragments arranged by size:
3171 1887 53 25
NspI r_CATG'y
Cuts at: 0 610 2799 5136
Size: 610 2189 2337
Fragments arranged by size:
2337 2189 610
PasI CC'CwG_GG
Cuts at: 0 204 383 420 601 1027 1102 1384 1576
Size: 204 179 37 181 426 75 282 192
Cuts at: 1576 1585 2153 2200 2299 2611 2917 3049 3067
Size: 9 568 47 99 312 306 132 18
Cuts at: 3067 3151 3223 3397 3964 3999 4043 4218 4237
Size: 84 72 174 567 35 44 175 19
Cuts at: 4237 4263 5136
Size: 26 873
Fragments arranged by size:
873 568 567 426 312 306 282 204
192 181 179 175 174 132 99 84
75 72 47 44 37 35 26 19
18 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 3977 4590 4917 5136
Size: 3977 613 327 219
Fragments arranged by size:
3977 613 327 219
PfoI T'CCnGG_A
Cuts at: 0 357 900 1299 1485 1620 1815 1842 2394
Size: 357 543 399 186 135 195 27 552
Cuts at: 2394 2511 2700 3432 3567 3621 3918 3954 5136
Size: 117 189 732 135 54 297 36 1182
Fragments arranged by size:
1182 732 552 543 399 357 297 195
189 186 135 135 117 54 36 27
PpuMI rG'GwC_Cy
Cuts at: 0 298 586 745 1003 1159 1219 1303 1498
Size: 298 288 159 258 156 60 84 195
Cuts at: 1498 1803 1961 2077 2194 2212 2266 2479 2607
Size: 305 158 116 117 18 54 213 128
Cuts at: 2607 2741 2913 4111 4213 4366 4587 5136
Size: 134 172 1198 102 153 221 549
Fragments arranged by size:
1198 549 305 298 288 258 221 213
195 172 159 158 156 153 134 128
117 116 102 84 60 54 18
PshAI GACnn'nnGTC
Cuts at: 0 431 2174 3418 5136
Size: 431 1743 1244 1718
Fragments arranged by size:
1743 1718 1244 431
PspOMI G'GGCC_C
Cuts at: 0 198 379 442 478 1030 1106 1266 1791
Size: 198 181 63 36 552 76 160 525
Cuts at: 1791 1854 1941 2050 2149 2293 2551 2920 3885
Size: 63 87 109 99 144 258 369 965
Cuts at: 3885 3994 4039 4258 4266 4312 5136
Size: 109 45 219 8 46 824
Fragments arranged by size:
965 824 552 525 369 258 219 198
181 160 144 109 109 99 87 76
63 63 46 45 36 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1821 1853 5136
Size: 1821 32 3283
Fragments arranged by size:
3283 1821 32
PstI C_TGCA'G
Cuts at: 0 251 5136
Size: 251 4885
PvuII CAG'CTG
Cuts at: 0 4840 4934 5136
Size: 4840 94 202
Fragments arranged by size:
4840 202 94
SacI G_AGCT'C
Cuts at: 0 636 2337 2538 3549 3756 3954 5136
Size: 636 1701 201 1011 207 198 1182
Fragments arranged by size:
1701 1182 1011 636 207 201 198
SacII CC_GC'GG
Cuts at: 0 1413 3588 4986 5136
Size: 1413 2175 1398 150
Fragments arranged by size:
2175 1413 1398 150
SanDI GG'GwC_CC
Cuts at: 0 745 1003 1219 1803 1961 2607 2741 2913
Size: 745 258 216 584 158 646 134 172
Cuts at: 2913 4111 4213 5136
Size: 1198 102 923
Fragments arranged by size:
1198 923 745 646 584 258 216 172
158 134 102
SapI GCTCTTCn'nnn_
Cuts at: 0 1365 3920 5136
Size: 1365 2555 1216
Fragments arranged by size:
2555 1365 1216
SexAI A'CCwGG_T
Cuts at: 0 3342 3558 3837 3900 4281 5136
Size: 3342 216 279 63 381 855
Fragments arranged by size:
3342 855 381 279 216 63
SfcI C'TryA_G
Cuts at: 0 247 5136
Size: 247 4889
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 477 1568 1766 1877 1886 1949 2246 2292
Size: 477 1091 198 111 9 63 297 46
Cuts at: 2292 2468 2495 5136
Size: 176 27 2641
Fragments arranged by size:
2641 1091 477 297 198 176 111 63
46 27 9
SfoI GGC'GCC
Cuts at: 0 3854 5136
Size: 3854 1282
SmaI CCC'GGG
Cuts at: 0 242 368 477 1000 1094 1148 1311 1704
Size: 242 126 109 523 94 54 163 393
Cuts at: 1704 2343 2748 2820 4146 4164 4427 5136
Size: 639 405 72 1326 18 263 709
Fragments arranged by size:
1326 709 639 523 405 393 263 242
163 126 109 94 72 54 18
SmlI C'TyrA_G
Cuts at: 0 924 1591 5077 5136
Size: 924 667 3486 59
Fragments arranged by size:
3486 924 667 59
SphI G_CATG'C
Cuts at: 0 2799 5136
Size: 2799 2337
SrfI GCCC'GGGC
Cuts at: 0 2343 4427 5136
Size: 2343 2084 709
Fragments arranged by size:
2343 2084 709
StuI AGG'CCT
Cuts at: 0 968 1202 2069 2105 2390 2471 2498 3236
Size: 968 234 867 36 285 81 27 738
Cuts at: 3236 3311 4008 5136
Size: 75 697 1128
Fragments arranged by size:
1128 968 867 738 697 285 234 81
75 36 27
StyI C'CwwG_G
Cuts at: 0 302 483 1007 1110 1223 1502 2054 2430
Size: 302 181 524 103 113 279 552 376
Cuts at: 2430 2555 2790 3030 3423 3611 3747 4106 4115
Size: 125 235 240 393 188 136 359 9
Cuts at: 4115 4317 5136
Size: 202 819
Fragments arranged by size:
819 552 524 393 376 359 302 279
240 235 202 188 181 136 125 113
103 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 824 4832 5136
Size: 824 4008 304
Fragments arranged by size:
4008 824 304
TatI w'GTAC_w
Cuts at: 0 818 1453 4553 4640 5012 5136
Size: 818 635 3100 87 372 124
Fragments arranged by size:
3100 818 635 372 124 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 456 488 1698 1730 1919 1951 2610 2642
Size: 456 32 1210 32 189 32 659 32
Cuts at: 2642 4492 4524 5136
Size: 1850 32 612
Fragments arranged by size:
1850 1210 659 612 456 189 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 179 5136
Size: 179 4957
XbaI T'CTAG_A
Cuts at: 0 4938 5136
Size: 4938 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 211 309 2232 2437 2797 3430 4006 4086
Size: 211 98 1923 205 360 633 576 80
Cuts at: 4086 4324 4387 4512 4728 5136
Size: 238 63 125 216 408
Fragments arranged by size:
1923 633 576 408 360 238 216 211
205 125 98 80 63
XmaI C'CCGG_G
Cuts at: 0 240 366 475 998 1092 1146 1309 1702
Size: 240 126 109 523 94 54 163 393
Cuts at: 1702 2341 2746 2818 4144 4162 4425 5136
Size: 639 405 72 1326 18 263 711
Fragments arranged by size:
1326 711 639 523 405 393 263 240
163 126 109 94 72 54 18
XmnI GAAnn'nnTTC
Cuts at: 0 3355 5136
Size: 3355 1781
Enzymes that do cut:
AarI AcuI AhdI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI
BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PfoI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BclI BfrBI BglII BmtI BplI Bpu10I BsaAI BsaWI
BsiWI BspEI BspHI BssSI BstBI BstZ17I Bsu36I ClaI
CspCI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PsiI PspXI PvuI RsrII
SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SspI
SwaI XhoI ZraI