(Circular) (Six-base) MAPSORT of: pFN21AB7018.seq Check: 8336 from: 1 to: 7621 pFN21AB7018 7621 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4687 4687 Size: 7621 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3201 4548 5431 278 Size: 53 83 186 936 1665 1347 883 2468 Fragments arranged by size: 2468 1665 1347 936 883 186 83 53 AccI GT'mk_AC Cuts at: 1937 4527 4845 4964 1937 Size: 2590 318 119 4594 Fragments arranged by size: 4594 2590 318 119 Acc65I G'GTAC_C Cuts at: 4942 4942 Size: 7621 AclI AA'CG_TT Cuts at: 1563 2825 5748 6121 1563 Size: 1262 2923 373 3063 Fragments arranged by size: 3063 2923 1262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5684 7068 7336 1632 Size: 4052 1384 268 1917 Fragments arranged by size: 4052 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2311 820 Size: 197 1294 6130 Fragments arranged by size: 6130 1294 197 AflIII A'CryG_T Cuts at: 1203 2289 4530 6520 7497 1203 Size: 1086 2241 1990 977 1327 Fragments arranged by size: 2241 1990 1327 1086 977 AgeI A'CCGG_T Cuts at: 6452 6452 Size: 7621 AhdI GACnn_n'nnGTC Cuts at: 4549 6350 4549 Size: 1801 5820 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2603 1184 Size: 1419 6202 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3903 3935 3903 Size: 32 7589 AlwNI CAG_nnn'CTG Cuts at: 3341 4091 4900 5364 6936 3341 Size: 750 809 464 1572 4026 Fragments arranged by size: 4026 1572 809 750 464 ApaI G_GGCC'C Cuts at: 3987 3987 Size: 7621 ApaLI G'TGCA_C Cuts at: 2293 2701 4907 5678 6834 2293 Size: 408 2206 771 1156 3080 Fragments arranged by size: 3080 2206 1156 771 408 ApoI r'AATT_y Cuts at: 1493 2600 2687 4030 4930 5156 5308 7417 1493 Size: 1107 87 1343 900 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 1107 900 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 2943 6175 160 Size: 872 1911 3232 1606 Fragments arranged by size: 3232 1911 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7621 AvaI C'yCGr_G Cuts at: 1870 1943 4946 1870 Size: 73 3003 4545 Fragments arranged by size: 4545 3003 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3112 3145 3112 Size: 33 7588 BamHI G'GATC_C Cuts at: 4951 5318 4951 Size: 367 7254 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2454 3155 4501 4942 Size: 325 217 627 234 433 701 1346 441 Cuts at: 4942 6397 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1346 701 627 441 433 325 234 217 BanII G_rGCy'C Cuts at: 1391 3312 3987 4940 1391 Size: 1921 675 953 4072 Fragments arranged by size: 4072 1921 953 675 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 7621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2394 4119 4299 4709 928 Size: 971 495 1725 180 410 3840 Fragments arranged by size: 3840 1725 971 495 410 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5812 5846 1903 Size: 34 3875 34 3678 Fragments arranged by size: 3875 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5513 6723 5513 Size: 1210 6411 BclI T'GATC_A Cuts at: 1548 2517 3727 4638 1548 Size: 969 1210 911 4531 Fragments arranged by size: 4531 1210 969 911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6232 136 Size: 107 122 71 631 605 4560 1525 Fragments arranged by size: 4560 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4211 7616 4211 Size: 3405 4216 BlpI GC'TnA_GC Cuts at: 4301 4661 5038 4301 Size: 360 377 6884 Fragments arranged by size: 6884 377 360 Bme1580I G_kGCm'C Cuts at: 1790 2297 2705 3880 3987 4911 5682 6838 1790 Size: 507 408 1175 107 924 771 1156 2573 Fragments arranged by size: 2573 1175 1156 924 771 507 408 107 BmgBI CAC'GTC Cuts at: 1318 2700 1318 Size: 1382 6239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5352 6310 442 Size: 416 53 83 399 24 3935 958 1753 Fragments arranged by size: 3935 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7621 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 4597 2031 Size: 2566 5055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2880 3804 4191 6281 1239 Size: 1641 924 387 2090 2579 Fragments arranged by size: 2579 2090 1641 924 387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2432 2503 3851 5746 6611 6909 2432 Size: 71 1348 1895 865 298 3144 Fragments arranged by size: 3144 1895 1348 865 298 71 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6284 882 Size: 617 4785 2219 Fragments arranged by size: 4785 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2292 3124 5412 493 Size: 711 1088 832 2288 2702 Fragments arranged by size: 2702 2288 1088 832 711 BsaBI GATnn'nnATC Cuts at: 4719 7615 4719 Size: 2896 4725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 3198 4545 5428 Size: 53 83 186 936 489 1176 1347 883 Cuts at: 5428 5810 6445 275 Size: 382 635 1451 Fragments arranged by size: 1451 1347 1176 936 883 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 6054 6452 6726 6873 7284 4653 Size: 1401 398 274 147 411 4990 Fragments arranged by size: 4990 1401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 2979 3009 1178 Size: 30 165 30 1164 30 382 30 5790 Fragments arranged by size: 5790 1164 382 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3689 4879 1186 Size: 2503 1190 3928 Fragments arranged by size: 3928 2503 1190 BseYI C'CCAG_C Cuts at: 4710 4893 6824 7320 4710 Size: 183 1931 496 5011 Fragments arranged by size: 5011 1931 496 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3401 3955 4077 1163 Size: 573 1665 554 122 4707 Fragments arranged by size: 4707 1665 573 554 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4617 5071 5832 6860 1540 Size: 275 186 2616 454 761 1028 2301 Fragments arranged by size: 2616 2301 1028 761 454 275 186 BsiHKAI G_wGCw'C Cuts at: 2297 2705 4787 4911 5682 5767 6838 2297 Size: 408 2082 124 771 85 1071 3080 Fragments arranged by size: 3080 2082 1071 771 408 124 85 BsmI GAATG_Cn' Cuts at: 3020 3449 4114 4614 5132 5225 3020 Size: 429 665 500 518 93 5416 Fragments arranged by size: 5416 665 518 500 429 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2112 2112 Size: 7621 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 2705 3312 3880 3987 4787 4911 Size: 399 507 408 607 568 107 800 124 Cuts at: 4911 4940 5682 5767 6838 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 607 568 507 408 399 124 107 85 29 BspEI T'CCGG_A Cuts at: 4653 7284 4653 Size: 2631 4990 BspHI T'CATG_A Cuts at: 2514 5510 7240 2514 Size: 2996 1730 2895 Fragments arranged by size: 2996 2895 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4687 4976 844 Size: 3843 289 3489 Fragments arranged by size: 3843 3489 289 BsrBI CCG'CTC Cuts at: 1394 1823 2370 5508 1394 Size: 429 547 3138 3507 Fragments arranged by size: 3507 3138 547 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6116 6290 65 Size: 1183 4868 174 1396 Fragments arranged by size: 4868 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2279 4612 6265 6452 1700 Size: 110 469 2333 1653 187 2869 Fragments arranged by size: 2869 2333 1653 469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7621 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7621 BssSI C'ACGA_G Cuts at: 3266 5681 6693 3266 Size: 2415 1012 4194 Fragments arranged by size: 4194 2415 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3600 4091 794 Size: 2806 491 4324 Fragments arranged by size: 4324 2806 491 BstBI TT'CG_AA Cuts at: 6425 6425 Size: 7621 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7621 BstYI r'GATC_y Cuts at: 1972 2211 2341 3048 4211 4720 4951 5318 5703 Size: 239 130 707 1163 509 231 367 385 Cuts at: 5703 5720 7172 7258 7270 7616 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1163 707 509 385 367 346 239 231 130 86 17 12 BstZ17I GTA'TAC Cuts at: 4528 4528 Size: 7621 BtgI C'CryG_G Cuts at: 513 1065 4839 513 Size: 552 3774 3295 Fragments arranged by size: 3774 3295 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6882 Fragments arranged by size: 6882 683 56 BtsI GCAGTG_nn' Cuts at: 3050 5137 5931 5951 3050 Size: 2087 794 20 4720 Fragments arranged by size: 4720 2087 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7621 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7586 DraI TTT'AAA Cuts at: 3529 4925 3529 Size: 1396 6225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2144 2402 6628 809 Size: 507 828 258 4226 1802 Fragments arranged by size: 4226 1802 828 507 258 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3840 5068 5957 Size: 54 1425 50 275 18 2010 1228 889 Cuts at: 5957 7586 8 Size: 1629 43 Fragments arranged by size: 2010 1629 1425 1228 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5068 1537 Size: 275 3256 4090 Fragments arranged by size: 4090 3256 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2080 2475 5551 1353 Size: 727 395 3076 3423 Fragments arranged by size: 3423 3076 727 395 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6208 6582 6728 387 Size: 1064 138 4619 374 146 1280 Fragments arranged by size: 4619 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2880 3804 4191 5684 6281 7068 7336 Size: 393 1248 924 387 1493 597 787 268 Cuts at: 7336 1239 Size: 1524 Fragments arranged by size: 1524 1493 1248 924 787 597 393 387 268 EcoO109I rG'GnC_Cy Cuts at: 3983 3983 Size: 7621 EcoRI G'AATT_C Cuts at: 2687 4930 5308 2687 Size: 2243 378 5000 Fragments arranged by size: 5000 2243 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4112 4666 4112 Size: 554 7067 FspI TGC'GCA Cuts at: 6127 6127 Size: 7621 HaeII r_GCGC'y Cuts at: 1678 2016 2025 6768 1678 Size: 338 9 4743 2531 Fragments arranged by size: 4743 2531 338 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2567 2599 4535 4567 4774 Size: 32 135 32 995 32 1936 32 207 Cuts at: 4774 4806 6262 6294 6336 6368 1373 Size: 32 1456 32 42 32 2626 Fragments arranged by size: 2626 1936 1456 995 207 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2365 2829 4965 5211 669 Size: 988 281 427 464 2136 246 3079 Fragments arranged by size: 3079 2136 988 464 427 281 246 HindIII A'AGCT_T Cuts at: 748 2351 748 Size: 1603 6018 HpaI GTT'AAC Cuts at: 5211 5211 Size: 7621 KasI G'GCGC_C Cuts at: 2021 2021 Size: 7621 KpnI G_GTAC'C Cuts at: 4946 4946 Size: 7621 MfeI C'AATT_G Cuts at: 5220 5220 Size: 7621 MluI A'CGCG_T Cuts at: 7497 7497 Size: 7621 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2574 4382 4827 6735 6919 1305 Size: 1269 1808 445 1908 184 2007 Fragments arranged by size: 2007 1908 1808 1269 445 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5799 Fragments arranged by size: 5799 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2796 3608 4160 4914 5579 5938 Size: 648 1161 469 812 552 754 665 359 Cuts at: 5938 6097 518 Size: 159 2042 Fragments arranged by size: 2042 1161 812 754 665 648 552 469 359 159 MspA1I CmG'CkG Cuts at: 1606 2409 5037 5714 6862 7107 1606 Size: 803 2628 677 1148 245 2120 Fragments arranged by size: 2628 2120 1148 803 677 245 NarI GG'CG_CC Cuts at: 2022 2022 Size: 7621 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7069 NdeI CA'TA_TG Cuts at: 387 387 Size: 7621 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7621 NotI GC'GGCC_GC Cuts at: 5068 5068 Size: 7621 NspI r_CATG'y Cuts at: 1132 1229 3280 4979 6524 1132 Size: 97 2051 1699 1545 2229 Fragments arranged by size: 2229 2051 1699 1545 97 PacI TTA_AT'TAA Cuts at: 3828 3828 Size: 7621 PasI CC'CwG_GG Cuts at: 1348 3775 1348 Size: 2427 5194 PciI A'CATG_T Cuts at: 6520 6520 Size: 7621 PflMI CCAn_nnn'nTGG Cuts at: 1108 3289 3410 1108 Size: 2181 121 5319 Fragments arranged by size: 5319 2181 121 PfoI T'CCnGG_A Cuts at: 2125 2214 3334 4603 2125 Size: 89 1120 1269 5143 Fragments arranged by size: 5143 1269 1120 89 PmeI GTTT'AAAC Cuts at: 4925 4925 Size: 7621 PmlI CAC'GTG Cuts at: 2292 2292 Size: 7621 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5690 5722 7203 7235 5690 Size: 32 1481 32 6076 Fragments arranged by size: 6076 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7621 PsiI TTA'TAA Cuts at: 4101 5191 4101 Size: 1090 6531 PspOMI G'GGCC_C Cuts at: 3983 3983 Size: 7621 PstI C_TGCA'G Cuts at: 830 4136 4820 4973 830 Size: 3306 684 153 3478 Fragments arranged by size: 3478 3306 684 153 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7621 SalI G'TCGA_C Cuts at: 1936 4963 1936 Size: 3027 4594 SapI GCTCTTCn'nnn_ Cuts at: 2080 2080 Size: 7621 SbfI CC_TGCA'GG Cuts at: 4973 4973 Size: 7621 ScaI AGT'ACT Cuts at: 4144 5869 7581 4144 Size: 1725 1712 4184 Fragments arranged by size: 4184 1725 1712 SexAI A'CCwGG_T Cuts at: 4314 4314 Size: 7621 SfcI C'TryA_G Cuts at: 826 1045 2768 2967 3435 4132 4816 4969 6104 Size: 219 1723 199 468 697 684 153 1135 Cuts at: 6104 6785 6976 826 Size: 681 191 1471 Fragments arranged by size: 1723 1471 1135 697 684 681 468 219 199 191 153 SfoI GGC'GCC Cuts at: 2023 2023 Size: 7621 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7621 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7621 SmaI CCC'GGG Cuts at: 1872 4948 1872 Size: 3076 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2311 2447 2482 3866 5725 6626 Size: 197 926 368 136 35 1384 1859 901 Cuts at: 6626 6888 820 Size: 262 1553 Fragments arranged by size: 1859 1553 1384 926 901 368 262 197 136 35 SnaBI TAC'GTA Cuts at: 493 3124 5412 493 Size: 2631 2288 2702 Fragments arranged by size: 2702 2631 2288 SpeI A'CTAG_T Cuts at: 152 152 Size: 7621 SphI G_CATG'C Cuts at: 1132 3280 4979 1132 Size: 2148 1699 3774 Fragments arranged by size: 3774 2148 1699 SspI AAT'ATT Cuts at: 5 52 3632 3949 4007 5545 5 Size: 47 3580 317 58 1538 2081 Fragments arranged by size: 3580 2081 1538 317 58 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7621 StyI C'CwwG_G Cuts at: 513 1065 2475 2502 2916 3931 4194 5060 513 Size: 552 1410 27 414 1015 263 866 3074 Fragments arranged by size: 3074 1410 1015 866 552 414 263 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3767 5648 5665 5818 6003 6448 325 Size: 1255 2187 1881 17 153 185 445 1498 Fragments arranged by size: 2187 1881 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3364 3655 Size: 274 80 33 51 1443 753 634 291 Cuts at: 3655 4142 4152 5867 7579 96 Size: 487 10 1715 1712 138 Fragments arranged by size: 1715 1712 1443 753 634 487 291 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 3405 3437 7581 7613 2103 Size: 32 1270 32 4144 32 2111 Fragments arranged by size: 4144 2111 1270 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4916 1316 Size: 3600 4021 XbaI T'CTAG_A Cuts at: 4957 4957 Size: 7621 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7621 XmaI C'CCGG_G Cuts at: 1870 4946 1870 Size: 3076 4545 XmnI GAAnn'nnTTC Cuts at: 5750 5750 Size: 7621 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3199 4546 5429 276 Size: 53 83 186 936 1665 1347 883 2468 Fragments arranged by size: 2468 1665 1347 936 883 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI