Restriction map for pFN21AB7018
 (Linear) (Six-base) MAPSORT of: KIBB7018.seq  Check: 201  from: 1  to: 2916



KIBB7018.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2682    2916 
   Size:       2682     234
 
AatII G_ACGT'C

Cuts at:      0    1196    2543    2916 
   Size:       1196    1347     373

  Fragments arranged by size: 

                1347    1196     373
 
AccI GT'mk_AC

Cuts at:      0    2522    2840    2916 
   Size:       2522     318      76

  Fragments arranged by size: 

                2522     318      76
 
AclI AA'CG_TT

Cuts at:      0     820    2916 
   Size:        820    2096
 
AflII C'TTAA_G

Cuts at:      0     306    2916 
   Size:        306    2610
 
AflIII A'CryG_T

Cuts at:      0     284    2525    2916 
   Size:        284    2241     391

  Fragments arranged by size: 

                2241     391     284
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2544    2916 
   Size:       2544     372
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     598    2916 
   Size:        598    2318
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1898    1930    2916 
   Size:       1898      32     986

  Fragments arranged by size: 

                1898     986      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1336    2086    2895    2916 
   Size:       1336     750     809      21

  Fragments arranged by size: 

                1336     809     750      21
 
ApaI G_GGCC'C

Cuts at:      0    1982    2916 
   Size:       1982     934
 
ApaLI G'TGCA_C

Cuts at:      0     288     696    2902    2916 
   Size:        288     408    2206      14

  Fragments arranged by size: 

                2206     408     288      14
 
ApoI r'AATT_y

Cuts at:      0     595     682    2025    2916 
   Size:        595      87    1343     891

  Fragments arranged by size: 

                1343     891     595      87
 
AseI AT'TA_AT

Cuts at:      0     938    2916 
   Size:        938    1978
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1107    1140    2916 
   Size:       1107      33    1776

  Fragments arranged by size: 

                1776    1107      33
 
BanI G'GyrC_C

Cuts at:      0      16     449    1150    2496    2916 
   Size:         16     433     701    1346     420

  Fragments arranged by size: 

                1346     701     433     420      16
 
BanII G_rGCy'C

Cuts at:      0    1307    1982    2916 
   Size:       1307     675     934

  Fragments arranged by size: 

                1307     934     675
 
BbeI G_GCGC'C

Cuts at:      0      20    2916 
   Size:         20    2896
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389    2114    2294    2704    2916 
   Size:        389    1725     180     410     212

  Fragments arranged by size: 

                1725     410     389     212     180
 
BclI T'GATC_A

Cuts at:      0     512    1722    2633    2916 
   Size:        512    1210     911     283

  Fragments arranged by size: 

                1210     911     512     283
 
BglII A'GATC_T

Cuts at:      0    2206    2916 
   Size:       2206     710
 
BlpI GC'TnA_GC

Cuts at:      0    2296    2656    2916 
   Size:       2296     360     260

  Fragments arranged by size: 

                2296     360     260
 
Bme1580I G_kGCm'C

Cuts at:      0     292     700    1875    1982    2906    2916 
   Size:        292     408    1175     107     924      10

  Fragments arranged by size: 

                1175     924     408     292     107      10
 
BmgBI CAC'GTC

Cuts at:      0     695    2916 
   Size:        695    2221
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      26    2592    2916 
   Size:         26    2566     324

  Fragments arranged by size: 

                2566     324      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    1799    2186    2916 
   Size:        875     924     387     730

  Fragments arranged by size: 

                 924     875     730     387
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     498    1846    2916 
   Size:        427      71    1348    1070

  Fragments arranged by size: 

                1348    1070     427      71
 
BsaAI yAC'GTr

Cuts at:      0     287    1119    2916 
   Size:        287     832    1797

  Fragments arranged by size: 

                1797     832     287
 
BsaBI GATnn'nnATC

Cuts at:      0    2714    2916 
   Size:       2714     202
 
BsaHI Gr'CG_yC

Cuts at:      0      17    1193    2540    2916 
   Size:         17    1176    1347     376

  Fragments arranged by size: 

                1347    1176     376      17
 
BsaWI w'CCGG_w

Cuts at:      0    2648    2916 
   Size:       2648     268
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     562     592     974    1004    2916 
   Size:        562      30     382      30    1912

  Fragments arranged by size: 

                1912     562     382      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1684    2874    2916 
   Size:       1684    1190      42

  Fragments arranged by size: 

                1684    1190      42
 
BseYI C'CCAG_C

Cuts at:      0    2705    2888    2916 
   Size:       2705     183      28

  Fragments arranged by size: 

                2705     183      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1396    1950    2072    2916 
   Size:       1396     554     122     844

  Fragments arranged by size: 

                1396     844     554     122
 
BsiEI CG_ry'CG

Cuts at:      0    2612    2916 
   Size:       2612     304
 
BsiHKAI G_wGCw'C

Cuts at:      0     292     700    2782    2906    2916 
   Size:        292     408    2082     124      10

  Fragments arranged by size: 

                2082     408     292     124      10
 
BsmI GAATG_Cn'

Cuts at:      0    1015    1444    2109    2609    2916 
   Size:       1015     429     665     500     307

  Fragments arranged by size: 

                1015     665     500     429     307
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     107    2916 
   Size:        107    2809
 
Bsp1286I G_dGCh'C

Cuts at:      0     292     700    1307    1875    1982    2782    2906    2916 
   Size:        292     408     607     568     107     800     124      10

  Fragments arranged by size: 

                 800     607     568     408     292     124     107      10
            
 
BspEI T'CCGG_A

Cuts at:      0    2648    2916 
   Size:       2648     268
 
BspHI T'CATG_A

Cuts at:      0     509    2916 
   Size:        509    2407
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2682    2916 
   Size:       2682     234
 
BsrBI CCG'CTC

Cuts at:      0     365    2916 
   Size:        365    2551
 
BsrFI r'CCGG_y

Cuts at:      0     274    2607    2916 
   Size:        274    2333     309

  Fragments arranged by size: 

                2333     309     274
 
BssSI C'ACGA_G

Cuts at:      0    1261    2916 
   Size:       1261    1655
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1595    2086    2916 
   Size:       1595     491     830

  Fragments arranged by size: 

                1595     830     491
 
BstYI r'GATC_y

Cuts at:      0     206     336    1043    2206    2715    2916 
   Size:        206     130     707    1163     509     201

  Fragments arranged by size: 

                1163     707     509     206     201     130
 
BstZ17I GTA'TAC

Cuts at:      0    2523    2916 
   Size:       2523     393
 
BtgI C'CryG_G

Cuts at:      0    2834    2916 
   Size:       2834      82
 
BtsI GCAGTG_nn'

Cuts at:      0    1045    2916 
   Size:       1045    1871
 
DraI TTT'AAA

Cuts at:      0    1524    2916 
   Size:       1524    1392
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     139     397    2916 
   Size:        139     258    2519

  Fragments arranged by size: 

                2519     258     139
 
EaeI y'GGCC_r

Cuts at:      0    1835    2916 
   Size:       1835    1081
 
EarI CTCTTCn'nnn_

Cuts at:      0      75     470    2916 
   Size:         75     395    2446

  Fragments arranged by size: 

                2446     395      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     875    1799    2186    2916 
   Size:        875     924     387     730

  Fragments arranged by size: 

                 924     875     730     387
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1978    2916 
   Size:       1978     938
 
EcoRI G'AATT_C

Cuts at:      0     682    2916 
   Size:        682    2234
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2107    2661    2916 
   Size:       2107     554     255

  Fragments arranged by size: 

                2107     554     255
 
HaeII r_GCGC'y

Cuts at:      0      11      20    2916 
   Size:         11       9    2896

  Fragments arranged by size: 

                2896      11       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594    2530    2562    2769    2801    2916 
   Size:        562      32    1936      32     207      32     115

  Fragments arranged by size: 

                1936     562     207     115      32      32      32
 
HincII GTy'rAC

Cuts at:      0     360     824    2916 
   Size:        360     464    2092

  Fragments arranged by size: 

                2092     464     360
 
HindIII A'AGCT_T

Cuts at:      0     346    2916 
   Size:        346    2570
 
KasI G'GCGC_C

Cuts at:      0      16    2916 
   Size:         16    2900
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     569    2377    2822    2916 
   Size:        569    1808     445      94

  Fragments arranged by size: 

                1808     569     445      94
 
MslI CAynn'nnrTG

Cuts at:      0     322     791    1603    2155    2909    2916 
   Size:        322     469     812     552     754       7

  Fragments arranged by size: 

                 812     754     552     469     322       7
 
MspA1I CmG'CkG

Cuts at:      0     404    2916 
   Size:        404    2512
 
NarI GG'CG_CC

Cuts at:      0      17    2916 
   Size:         17    2899
 
NspI r_CATG'y

Cuts at:      0    1275    2916 
   Size:       1275    1641
 
PacI TTA_AT'TAA

Cuts at:      0    1823    2916 
   Size:       1823    1093
 
PasI CC'CwG_GG

Cuts at:      0    1770    2916 
   Size:       1770    1146
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1284    1405    2916 
   Size:       1284     121    1511

  Fragments arranged by size: 

                1511    1284     121
 
PfoI T'CCnGG_A

Cuts at:      0     120     209    1329    2598    2916 
   Size:        120      89    1120    1269     318

  Fragments arranged by size: 

                1269    1120     318     120      89
 
PmlI CAC'GTG

Cuts at:      0     287    2916 
   Size:        287    2629
 
PsiI TTA'TAA

Cuts at:      0    2096    2916 
   Size:       2096     820
 
PspOMI G'GGCC_C

Cuts at:      0    1978    2916 
   Size:       1978     938
 
PstI C_TGCA'G

Cuts at:      0    2131    2815    2916 
   Size:       2131     684     101

  Fragments arranged by size: 

                2131     684     101
 
SapI GCTCTTCn'nnn_

Cuts at:      0      75    2916 
   Size:         75    2841
 
ScaI AGT'ACT

Cuts at:      0    2139    2916 
   Size:       2139     777
 
SexAI A'CCwGG_T

Cuts at:      0    2309    2916 
   Size:       2309     607
 
SfcI C'TryA_G

Cuts at:      0     763     962    1430    2127    2811    2916 
   Size:        763     199     468     697     684     105

  Fragments arranged by size: 

                 763     697     684     468     199     105
 
SfoI GGC'GCC

Cuts at:      0      18    2916 
   Size:         18    2898
 
SmlI C'TyrA_G

Cuts at:      0     306     442     477    1861    2916 
   Size:        306     136      35    1384    1055

  Fragments arranged by size: 

                1384    1055     306     136      35
 
SnaBI TAC'GTA

Cuts at:      0    1119    2916 
   Size:       1119    1797
 
SphI G_CATG'C

Cuts at:      0    1275    2916 
   Size:       1275    1641
 
SspI AAT'ATT

Cuts at:      0    1627    1944    2002    2916 
   Size:       1627     317      58     914

  Fragments arranged by size: 

                1627     914     317      58
 
StyI C'CwwG_G

Cuts at:      0     470     497     911    1926    2189    2916 
   Size:        470      27     414    1015     263     727

  Fragments arranged by size: 

                1015     727     470     414     263      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1762    2916 
   Size:       1762    1154
 
TatI w'GTAC_w

Cuts at:      0     725    1359    1650    2137    2147    2916 
   Size:        725     634     291     487      10     769

  Fragments arranged by size: 

                 769     725     634     487     291      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130    1400    1432    2916 
   Size:         98      32    1270      32    1484

  Fragments arranged by size: 

                1484    1270      98      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2911    2916 
   Size:       2911       5
 
ZraI GAC'GTC

Cuts at:      0    1194    2541    2916 
   Size:       1194    1347     375

  Fragments arranged by size: 

                1347    1194     375
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     BaeI     BanI
    BanII     BbeI     BbsI     BclI    BglII     BlpI Bme1580I    BmgBI
     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstYI
  BstZ17I     BtgI     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     MmeI     MslI   MspA1I     NarI     NspI     PacI     PasI    PflMI
     PfoI     PmlI     PsiI   PspOMI     PstI     SapI     ScaI    SexAI
     SfcI     SfoI     SmlI    SnaBI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

   Acc65I     AcuI     AfeI     AgeI     AleI     AscI    AsiSI     AvaI
    AvrII    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI     BmrI
     BmtI   Bpu10I     BsaI    BsiWI    BsrDI    BsrGI   BssHII    BstBI
   BstEII    BstXI   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PciI     PmeI     PpiI    PpuMI
    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI     SpeI
     SrfI     StuI     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI
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