Restriction map for
pFN21AB5645
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AclI AflII Aor51HI AscI
AseI AsnI Asp718I AspI AvrII
BfrI BlnI BlpI Bpu1102I BsaBI
BsiWI BspHI BssHII Bst1107I BstBI
BstZ17I CelII CpoI DrdI Eco47III
EcoNI EcoRV FseI HindIII HpaI
KpnI KspI MamI MluI NdeI
NheI NruI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 538 GACGTC GACGT^C
AccIII 2523 TCCGGA T^CCGGA
AflIII 1348 ACATGT A^C[GA][CT]GT
AgeI 1817 ACCGGT A^CCGGT
AhdI 17 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1755 GTGCAC G^TGCAC
ApaLI 1755 GTGCAC G^TGCAC
Asp700I 3306 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 17 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1373 GGATCC G^GATCC
BbeI 134 GGCGCC GGCGC^C
BbrPI 415 CACGTG CAC^GTG
BclI 1364 TGATCA T^GATCA
BglI 2638 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1616 AGATCT A^GATCT
BsaAI 415 CACGTG [CT]AC^GT[GA]
BseAI 2523 TCCGGA T^CCGGA
BsiEI 1206 CGGCCG CG[GA][CT]^CG
Bsp1407I 500 TGTACA T^GTACA
BspDI 2824 ATCGAT AT^CGAT
BspEI 2523 TCCGGA T^CCGGA
BspLU11I 1348 ACATGT A^CATGT
BsrGI 500 TGTACA T^GTACA
BstEII 1493 GGTCACC G^GT[ACGT]ACC
BstPI 1493 GGTCACC G^GT[ACGT]ACC
BstXI 563 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2824 ATCGAT AT^CGAT
DraI 1294 TTTAAA TTT^AAA
EagI 1206 CGGCCG C^GGCCG
Eam1105I 17 GACTAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1206 CGGCCG C^GGCCG
Eco52I 1206 CGGCCG C^GGCCG
EcoO65I 1493 GGTCACC G^GT[ACGT]ACC
EcoRI 1193 GAATTC G^AATTC
EcoT22I 37 ATGCAT ATGCA^T
FbaI 1364 TGATCA T^GATCA
HaeII 134 GGCGCC [GA]GCGC^[CT]
KasI 134 GGCGCC G^GCGCC
MroI 2523 TCCGGA T^CCGGA
NaeI 1672 GCCGGC GCC^GGC
NarI 134 GGCGCC GG^CGCC
NgoMIV 1672 GCCGGC G^CCGGC
NotI 1205 GCGGCCGC GC^GGCCGC
NsiI 37 ATGCAT ATGCA^T
PinAI 1817 ACCGGT A^CCGGT
PmaCI 415 CACGTG CAC^GTG
PmlI 415 CACGTG CAC^GTG
SapI 459 GAAGAGC GCTCTTC(1/4)
SfoI 134 GGCGCC GGC^GCC
SgrAI 1816 CACCGGTG C[GA]^CCGG[CT]G
SmaI 299 CCCGGG CCC^GGG
SmlI 3461 CTCAAG C^T[CT][GA]AG
SspBI 500 TGTACA T^GTACA
XmaCI 299 CCCGGG C^CCGGG
XmaI 299 CCCGGG C^CCGGG
XmnI 3306 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2446 GTCTAC GT^[AC][GT]AC
3437 GTAGAC
AccII 1204 CGCG CG^CG
1433 CGCG
AcsI 1193 GAATTC [GA]^AATT[CT]
2237 AAATTC
AocI 279 CCTGAGG CC^T[ACGT]AGG
678 CCTCAGG
ApaI 150 GGGCCC GGGCC^C
491 GGGCCC
ApoI 1193 GAATTC [GA]^AATT[CT]
2237 AAATTC
AvaI 299 CCCGGG C^[CT]CG[GA]G
3174 CTCGGG
AviII 860 TGCGCA TGC^GCA
3269 TGCGCA
BbvCI 387 CCTCAGC CCTCAGC(-5/-2)
933 GCTGAGG
BcgI 3057 CGATGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3282 GCATGACCCTCG
BciVI 3169 GGATAC GTATCC(6/5)
2414 GTATCC
BsaWI 1817 ACCGGT [AT]^CCGG[AT]
2523 TCCGGA
BsoBI 299 CCCGGG C^[CT]CG[GA]G
3174 CTCGGG
BspMI 1264 ACCTGC ACCTGC(4/8)
3224 ACCTGC
BsrBI 1209 CCGCTC CCGCTC(-3/-3)
322 GAGCGG
BsrDI 1187 GCAATG GCAATG(2/0)
2051 GCAATG
BssSI 3156 CACGAG CACGAG(-5/-1)
2734 CTCGTG
BstUI 1204 CGCG CG^CG
1433 CGCG
Bsu36I 279 CCTGAGG CC^T[ACGT]AGG
678 CCTCAGG
EarI 459 GAAGAG CTCTTC(1/4)
907 GAAGAG
Eco81I 279 CCTGAGG CC^T[ACGT]AGG
678 CCTCAGG
FspI 860 TGCGCA TGC^GCA
3269 TGCGCA
HincII 2848 GTTGAC GT[CT]^[GA]AC
2968 GTCAAC
HindII 2848 GTTGAC GT[CT]^[GA]AC
2968 GTCAAC
Ksp632I 459 GAAGAG CTCTTC(1/4)
907 GAAGAG
MfeI 1640 CAATTG C^AATTG
2502 CAATTG
MseI 1295 TTAA T^TAA
3008 TTAA
MunI 1640 CAATTG C^AATTG
2502 CAATTG
MvnI 1204 CGCG CG^CG
1433 CGCG
SexAI 230 ACCTGGT A^CC[AT]GGT
2105 ACCTGGT
TfiI 2876 GATTC G^A[AT]TC
2886 GATTC
Tru9I 1295 TTAA T^TAA
3008 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII