Restriction map for
pFN21AB5530
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaII AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MfeI MflI MluI MroI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TspRI Tth111I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 76 GACGCC G[GA]^CG[CT]C
AluI 86 AGCT AG^CT
AlwI 180 GGATC GGATC(4/5)
AvaI 112 CCCGGG C^[CT]CG[GA]G
BalI 65 TGGCCA TGG^CCA
BanI 253 GGCACC G^G[CT][GA]CC
BbsI 265 GAAGAC GAAGAC(2/6)
BfaI 245 CTAG C^TAG
BpuAI 265 GAAGAC GAAGAC(2/6)
BsaBI 351 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 76 GACGCC G[GA]^CG[CT]C
BsmFI 118 GGGAC GGGAC(10/14)
BsoBI 112 CCCGGG C^[CT]CG[GA]G
BspHI 93 TCATGA T^CATGA
BsrFI 13 GCCGGC [GA]^CCGG[CT]
Cac8I 13 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 13 GCCGGC [GA]^CCGG[CT]
DpnII 181 GATC ^GATC
EarI 124 CTCTTC CTCTTC(1/4)
EcoT14I 197 CCATGG C^C[AT][AT]GG
FauI 56 CCCGC CCCGC(4/6)
Hin1I 76 GACGCC G[GA]^CG[CT]C
Ksp632I 124 CTCTTC CTCTTC(1/4)
MaeI 245 CTAG C^TAG
MaeIII 241 GTCAC ^GT[ACGT]AC
MamI 351 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 181 GATC ^GATC
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
MseI 259 TTAA T^TAA
MspA1I 18 CAGCGG C[AC]G^C[GT]G
MwoI 35 GCCAGAGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 13 GCCGGC GCC^GGC
NcoI 197 CCATGG C^CATGG
NdeII 181 GATC ^GATC
NgoMIV 13 GCCGGC G^CCGGC
NlaIV 253 GGCACC GG[ACGT]^[ACGT]CC
NspI 321 ACATGC [GA]CATG^[CT]
RcaI 93 TCATGA T^CATGA
SapI 123 GCTCTTC GCTCTTC(1/4)
Sau3AI 181 GATC ^GATC
SfaNI 337 GATGC GCATC(5/9)
SmaI 112 CCCGGG CCC^GGG
StyI 197 CCATGG C^C[AT][AT]GG
TfiI 152 GAATC G^A[AT]TC
Tru9I 259 TTAA T^TAA
Tsp45I 241 GTCAC ^GT[GC]AC
XcmI 53 CCACCCGCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 112 CCCGGG C^CCGGG
XmaI 112 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 43 CGCG CG^CG
81 CGCG
BbvI 17 GCAGC GCAGC(8/12)
84 GCAGC
BsiYI 57 CCGCACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
107 CCACGCCCGGG
BslI 57 CCGCACCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
107 CCACGCCCGGG
BstF5I 48 CATCC GGATG(2/0)
333 GGATG
BstNI 235 CCAGG CC^[AT]GG
250 CCTGG
BstUI 43 CGCG CG^CG
81 CGCG
CfoI 44 GCGC GCG^C
82 GCGC
DdeI 203 CTCAG C^T[ACGT]AG
341 CTCAG
DsaI 62 CCGTGG C^C[GA][CT]GG
197 CCATGG
EaeI 21 CGGCCA [CT]^GGCC[GA]
65 TGGCCA
EcoRII 235 CCAGG ^CC[AT]GG
250 CCTGG
FokI 48 CATCC GGATG(9/13)
333 GGATG
HaeIII 22 GGCC GG^CC
66 GGCC
HgaI 76 GACGC GACGC(5/10)
120 GACGC
HhaI 44 GCGC GCG^C
82 GCGC
HinP1I 44 GCGC G^CGC
82 GCGC
MboII 265 GAAGA GAAGA(8/7)
125 TCTTC
MvaI 235 CCAGG CC^[AT]GG
250 CCTGG
MvnI 43 CGCG CG^CG
81 CGCG
PleI 268 GACTC GAGTC(4/5)
239 GAGTC
TaqI 150 TCGA T^CGA
347 TCGA
TseI 17 GCAGC G^C[AT]GC
84 GCAGC
TthHB8I 150 TCGA T^CGA
347 TCGA
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AciI BcnI BsaJI BssKI Fnu4HI
HapII HinfI HpaII ItaI MnlI
MspI NciI NlaIII ScrFI Tsp509I