Restriction map for pFN21AB5266
 (Circular) (Six-base) MAPSORT of: pFN21AB5266.seq  Check: 4696  from: 1  to: 5755



pFN21AB5266  5755 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2039    2039 
   Size:       5755
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3565     278 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3098    1937 
   Size:       1161    4594
 
Acc65I G'GTAC_C

Cuts at:   3076    3076 
   Size:       5755
 
AclI AA'CG_TT

Cuts at:   1563    3882    4255    1563 
   Size:       2319     373    3063

  Fragments arranged by size: 

                3063    2319     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2053    2421    2949    2991    3018    3818    5202    5470 
   Size:        421     368     528      42      27     800    1384     268

Cuts at:   5470    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384     800     528     421     368     268      42
                  27
 
AfeI AGC'GCT

Cuts at:   2107    2107 
   Size:       5755
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5558
 
AflIII A'CryG_T

Cuts at:   1203    4654    5631    1203 
   Size:       3451     977    1327

  Fragments arranged by size: 

                3451    1327     977
 
AgeI A'CCGG_T

Cuts at:   4586    4586 
   Size:       5755
 
AhdI GACnn_n'nnGTC

Cuts at:   4484    4484 
   Size:       5755
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5755
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5755
 
AlwNI CAG_nnn'CTG

Cuts at:   2237    3041    3498    5070    2237 
   Size:        804     457    1572    2922

  Fragments arranged by size: 

                2922    1572     804     457
 
ApaLI G'TGCA_C

Cuts at:   3812    4968    3812 
   Size:       1156    4599
 
ApoI r'AATT_y

Cuts at:   1493    2545    2711    3013    3064    3290    3442    5551    1493 
   Size:       1052     166     302      51     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1052     302     226     166     152      51
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4309     160 
   Size:        872    3277    1606

  Fragments arranged by size: 

                3277    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3080    1870 
   Size:         73    1137    4545

  Fragments arranged by size: 

                4545    1137      73
 
BamHI G'GATC_C

Cuts at:   3085    3452    3085 
   Size:        367    5388
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3076    4531     618 
   Size:        325     217     627    1289    1455    1842

  Fragments arranged by size: 

                1842    1455    1289     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3074    1391 
   Size:       1683    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4784
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3946    3980    1903 
   Size:         34    2009      34    3678

  Fragments arranged by size: 

                3678    2009      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3647    4857    3647 
   Size:       1210    4545
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5755
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4366     136 
   Size:        107     122      71     631     605    2694    1525

  Fragments arranged by size: 

                2694    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5750    5750 
   Size:       5755
 
BlpI GC'TnA_GC

Cuts at:   3172    3172 
   Size:       5755
 
Bme1580I G_kGCm'C

Cuts at:   1790    3816    4972    1790 
   Size:       2026    1156    2573

  Fragments arranged by size: 

                2573    2026    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5755
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3486    4444     442 
   Size:        416      53      83     399      24    2069     958    1753

  Fragments arranged by size: 

                2069    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5755
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4415    1239 
   Size:       3176    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3880    4745    5043    3880 
   Size:        865     298    4592

  Fragments arranged by size: 

                4592     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4418     882 
   Size:        617    2919    2219

  Fragments arranged by size: 

                2919    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3546     493 
   Size:        711    2342    2702

  Fragments arranged by size: 

                2702    2342     711
 
BsaBI GATnn'nnATC

Cuts at:   5749    5749 
   Size:       5755
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2021    3562    3944    4579 
   Size:         53      83     186     936     488    1541     382     635

Cuts at:   4579     275 
   Size:       1451

  Fragments arranged by size: 

                1541    1451     936     635     488     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4188    4586    4860    5007    5418    4188 
   Size:        398     274     147     411    4525

  Fragments arranged by size: 

                4525     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5530

  Fragments arranged by size: 

                5530     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3002    1186 
   Size:       1816    3939
 
BseYI C'CCAG_C

Cuts at:   4958    5454    4958 
   Size:        496    5259
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2479    2655    1163 
   Size:        573     743     176    4263

  Fragments arranged by size: 

                4263     743     573     176
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3205    3966    4994    1540 
   Size:        275     186    1204     761    1028    2301

  Fragments arranged by size: 

                2301    1204    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3816    3901    4972    3816 
   Size:         85    1071    4599

  Fragments arranged by size: 

                4599    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3266    3359    3266 
   Size:         93    5662
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3074    3816    3901    4972    1391 
   Size:        399    1284     742      85    1071    2174

  Fragments arranged by size: 

                2174    1284    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5418    5418 
   Size:       5755
 
BspHI T'CATG_A

Cuts at:   3644    5374    3644 
   Size:       1730    4025
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2039    3110     844 
   Size:       1195    1071    3489

  Fragments arranged by size: 

                3489    1195    1071
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2026    3642    1394 
   Size:        429     203    1616    3507

  Fragments arranged by size: 

                3507    1616     429     203
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2016    4250    4424      65 
   Size:       1183     768    2234     174    1396

  Fragments arranged by size: 

                2234    1396    1183     768     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4399    4586    1700 
   Size:        110    2589     187    2869

  Fragments arranged by size: 

                2869    2589     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5755
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5755
 
BssSI C'ACGA_G

Cuts at:   3815    4827    3815 
   Size:       1012    4743
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5755
 
BstBI TT'CG_AA

Cuts at:   4559    4559 
   Size:       5755
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5755
 
BstYI r'GATC_y

Cuts at:   1972    3085    3452    3837    3854    5306    5392    5404    5750 
   Size:       1113     367     385      17    1452      86      12     346

Cuts at:   5750    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1113     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2412    2583    2412 
   Size:        171    5584
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2052     513 
   Size:        552     939      48    4216

  Fragments arranged by size: 

                4216     939     552      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5016

  Fragments arranged by size: 

                5016     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2467    3271    4065    4085    2467 
   Size:        804     794      20    4137

  Fragments arranged by size: 

                4137     804     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5755
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5720
 
DraI TTT'AAA

Cuts at:   2740    3059    2740 
   Size:        319    5436
 
DraIII CAC_nnn'GTG

Cuts at:   2815    2815 
   Size:       5755
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2799    4762     809 
   Size:        507    1483    1963    1802

  Fragments arranged by size: 

                1963    1802    1483     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2038    3202    4091 
   Size:         54    1425      50     275      18     208    1164     889

Cuts at:   4091    5720       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1164     889     275     208      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3202    1537 
   Size:        275    1390    4090

  Fragments arranged by size: 

                4090    1390     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2700    2979    3685    1353 
   Size:       1347     279     706    3423

  Fragments arranged by size: 

                3423    1347     706     279
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4342    4716    4862     387 
   Size:       1064     138    2753     374     146    1280

  Fragments arranged by size: 

                2753    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2053    2421    2949    2991    3018    3818    4415 
   Size:        393     421     368     528      42      27     800     597

Cuts at:   4415    5202    5470    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     800     787     597     528     421     393     368
                 268      42      27
 
EcoRI G'AATT_C

Cuts at:   3013    3064    3442    3013 
   Size:         51     378    5326

  Fragments arranged by size: 

                5326     378      51
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2732    2812    2732 
   Size:         80    5675
 
FspI TGC'GCA

Cuts at:   4261    4261 
   Size:       5755
 
HaeII r_GCGC'y

Cuts at:   1678    2109    4902    1678 
   Size:        431    2793    2531

  Fragments arranged by size: 

                2793    2531     431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2255    2281    2287    2313    2809 
   Size:         32     135      32     683      26       6      26     496

Cuts at:   2809    2841    4396    4428    4470    4502    1373 
   Size:         32    1555      32      42      32    2626

  Fragments arranged by size: 

                2626    1555     683     496     135      42      32      32
                  32      32      32      26      26       6
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2352    3099    3345     669 
   Size:        988     281     414     747     246    3079

  Fragments arranged by size: 

                3079     988     747     414     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5755
 
HpaI GTT'AAC

Cuts at:   2352    3345    2352 
   Size:        993    4762
 
KpnI G_GTAC'C

Cuts at:   3080    3080 
   Size:       5755
 
MfeI C'AATT_G

Cuts at:   3354    3354 
   Size:       5755
 
MluI A'CGCG_T

Cuts at:   5631    5631 
   Size:       5755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4869    5053    1305 
   Size:       3564     184    2007

  Fragments arranged by size: 

                3564    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2040      10 
   Size:         54    1425     343     208    3725

  Fragments arranged by size: 

                3725    1425     343     208      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    2181    3713    4072    4231     518 
   Size:        648     843     172    1532     359     159    2042

  Fragments arranged by size: 

                2042    1532     843     648     359     172     159
 
MspA1I CmG'CkG

Cuts at:   1606    2237    3171    3848    4996    5241    1606 
   Size:        631     934     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     934     677     631     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4264

  Fragments arranged by size: 

                4264     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5755
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5755
 
NotI GC'GGCC_GC

Cuts at:   3202    3202 
   Size:       5755
 
NspI r_CATG'y

Cuts at:   1132    1229    3113    4658    1132 
   Size:         97    1884    1545    2229

  Fragments arranged by size: 

                2229    1884    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5755
 
PciI A'CATG_T

Cuts at:   4654    4654 
   Size:       5755
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2259    2963    1108 
   Size:       1151     704    3900

  Fragments arranged by size: 

                3900    1151     704
 
PmeI GTTT'AAAC

Cuts at:   3059    3059 
   Size:       5755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3824    3856    5337    5369    3824 
   Size:         32    1481      32    4210

  Fragments arranged by size: 

                4210    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5755
 
PsiI TTA'TAA

Cuts at:   2603    2748    2760    3325    2603 
   Size:        145      12     565    5033

  Fragments arranged by size: 

                5033     565     145      12
 
PstI C_TGCA'G

Cuts at:    830    2242    3107     830 
   Size:       1412     865    3478

  Fragments arranged by size: 

                3478    1412     865
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5755
 
PvuII CAG'CTG

Cuts at:   2237    2237 
   Size:       5755
 
SalI G'TCGA_C

Cuts at:   1936    3097    1936 
   Size:       1161    4594
 
SbfI CC_TGCA'GG

Cuts at:   3107    3107 
   Size:       5755
 
ScaI AGT'ACT

Cuts at:   4003    5715    4003 
   Size:       1712    4043
 
SfcI C'TryA_G

Cuts at:    826    1045    2229    2238    3103    4238    4919    5110     826 
   Size:        219    1184       9     865    1135     681     191    1471

  Fragments arranged by size: 

                1471    1184    1135     865     681     219     191       9
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5755
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5755
 
SmaI CCC'GGG

Cuts at:   1872    3082    1872 
   Size:       1210    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3859    4760    5022     820 
   Size:        197     926    1916     901     262    1553

  Fragments arranged by size: 

                1916    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3546     493 
   Size:       3053    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5755
 
SphI G_CATG'C

Cuts at:   1132    3113    1132 
   Size:       1981    3774
 
SspI AAT'ATT

Cuts at:      5      52    3679       5 
   Size:         47    3627    2081

  Fragments arranged by size: 

                3627    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5755
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3194     513 
   Size:        552     939    1190    3074

  Fragments arranged by size: 

                3074    1190     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3782    3799    3952    4137    4582     325 
   Size:       1255    2202      17     153     185     445    1498

  Fragments arranged by size: 

                2202    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4001    5713      96 
   Size:        274      80      33      51    1443    2024    1712     138

  Fragments arranged by size: 

                2024    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5715    5747    5715 
   Size:         32    5723
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5755
 
XbaI T'CTAG_A

Cuts at:   3091    3091 
   Size:       5755
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5755
 
XmaI C'CCGG_G

Cuts at:   1870    3080    1870 
   Size:       1210    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2577    3884    2577 
   Size:       1307    4448
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3563     276 
   Size:         53      83     186     936    2029    2468

  Fragments arranged by size: 

                2468    2029     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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