(Circular) (Six-base) MAPSORT of: pFN21AB5115.seq Check: 5624 from: 1 to: 7996 pFN21AB5115 7996 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4159 4159 Size: 7996 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5806 278 Size: 53 83 186 936 4270 2468 Fragments arranged by size: 4270 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2745 2829 5339 1937 Size: 808 84 2510 4594 Fragments arranged by size: 4594 2510 808 84 Acc65I G'GTAC_C Cuts at: 5317 5317 Size: 7996 AclI AA'CG_TT Cuts at: 1563 6123 6496 1563 Size: 4560 373 3063 Fragments arranged by size: 4560 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2535 4670 4917 6059 7443 7711 1632 Size: 903 2135 247 1142 1384 268 1917 Fragments arranged by size: 2135 1917 1384 1142 903 268 247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7799 AflIII A'CryG_T Cuts at: 1203 2091 2834 3139 3151 5070 6895 7872 1203 Size: 888 743 305 12 1919 1825 977 1327 Fragments arranged by size: 1919 1825 1327 977 888 743 305 12 AgeI A'CCGG_T Cuts at: 6827 6827 Size: 7996 AhdI GACnn_n'nnGTC Cuts at: 5071 6725 5071 Size: 1654 6342 AleI CACnn'nnGTG Cuts at: 1166 2774 1166 Size: 1608 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7996 AlwNI CAG_nnn'CTG Cuts at: 2190 2513 5739 7311 2190 Size: 323 3226 1572 2875 Fragments arranged by size: 3226 2875 1572 323 ApaI G_GGCC'C Cuts at: 2648 3879 2648 Size: 1231 6765 ApaLI G'TGCA_C Cuts at: 6053 7209 6053 Size: 1156 6840 ApoI r'AATT_y Cuts at: 1493 2558 3539 4615 4891 5305 5531 5683 7792 Size: 1065 981 1076 276 414 226 152 2109 Cuts at: 7792 1493 Size: 1697 Fragments arranged by size: 2109 1697 1076 1065 981 414 276 226 152 AseI AT'TA_AT Cuts at: 160 1032 6550 160 Size: 872 5518 1606 Fragments arranged by size: 5518 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7996 AvaI C'yCGr_G Cuts at: 1870 1943 5321 1870 Size: 73 3378 4545 Fragments arranged by size: 4545 3378 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2737 2770 2737 Size: 33 7963 BamHI G'GATC_C Cuts at: 5326 5693 5326 Size: 367 7629 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3926 4034 5317 6772 618 Size: 325 217 627 2139 108 1283 1455 1842 Fragments arranged by size: 2139 1842 1455 1283 627 325 217 108 BanII G_rGCy'C Cuts at: 1391 2465 2648 3879 5110 5315 1391 Size: 1074 183 1231 1231 205 4072 Fragments arranged by size: 4072 1231 1231 1074 205 183 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 2474 2957 928 Size: 971 123 452 483 5967 Fragments arranged by size: 5967 971 483 452 123 BbvCI CC'TCA_GC Cuts at: 2136 2367 2136 Size: 231 7765 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5090 5124 6187 6221 1903 Size: 34 3153 34 1063 34 3678 Fragments arranged by size: 3678 3153 1063 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5888 7098 5888 Size: 1210 6786 BclI T'GATC_A Cuts at: 1548 2689 3007 4858 1548 Size: 1141 318 1851 4686 Fragments arranged by size: 4686 1851 1141 318 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6607 136 Size: 107 122 71 631 605 4935 1525 Fragments arranged by size: 4935 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2419 7991 2419 Size: 5572 2424 BlpI GC'TnA_GC Cuts at: 5413 5413 Size: 7996 Bme1580I G_kGCm'C Cuts at: 1790 2648 3879 3931 4039 4498 6057 7213 1790 Size: 858 1231 52 108 459 1559 1156 2573 Fragments arranged by size: 2573 1559 1231 1156 858 459 108 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7996 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2178 5727 6685 Size: 416 53 83 399 24 761 3549 958 Cuts at: 6685 442 Size: 1753 Fragments arranged by size: 3549 1753 958 761 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7996 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4600 4895 4600 Size: 295 7701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2379 2460 2812 6656 1239 Size: 816 324 81 352 3844 2579 Fragments arranged by size: 3844 2579 816 352 324 81 Bpu10I CC'TnA_GC Cuts at: 2136 2367 5172 2136 Size: 231 2805 4960 Fragments arranged by size: 4960 2805 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2923 5268 6121 6986 7284 2923 Size: 2345 853 865 298 3635 Fragments arranged by size: 3635 2345 865 853 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4632 6659 882 Size: 617 3133 2027 2219 Fragments arranged by size: 3133 2219 2027 617 BsaAI yAC'GTr Cuts at: 493 1204 5787 493 Size: 711 4583 2702 Fragments arranged by size: 4583 2702 711 BsaBI GATnn'nnATC Cuts at: 7990 7990 Size: 7996 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5803 6185 6820 275 Size: 53 83 186 936 4270 382 635 1451 Fragments arranged by size: 4270 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6429 6827 7101 7248 7659 6429 Size: 398 274 147 411 6766 Fragments arranged by size: 6766 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5169 5199 1178 Size: 30 165 30 3766 30 3975 Fragments arranged by size: 3975 3766 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2052 2055 3716 3780 3825 4059 4197 4245 Size: 866 3 1661 64 45 234 138 48 Cuts at: 4245 4293 4341 4389 4437 1186 Size: 48 48 48 48 4745 Fragments arranged by size: 4745 1661 866 234 138 64 48 48 48 48 48 45 3 BseYI C'CCAG_C Cuts at: 2726 7199 7695 2726 Size: 4473 496 3027 Fragments arranged by size: 4473 3027 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2598 3034 4953 1163 Size: 573 862 436 1919 4206 Fragments arranged by size: 4206 1919 862 573 436 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5446 6207 7235 1540 Size: 275 186 3445 761 1028 2301 Fragments arranged by size: 3445 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2395 2465 6057 6142 7213 2395 Size: 70 3592 85 1071 3178 Fragments arranged by size: 3592 3178 1071 85 70 BsmI GAATG_Cn' Cuts at: 5507 5600 5507 Size: 93 7903 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2465 2648 3879 3931 4039 4498 Size: 399 605 70 183 1231 52 108 459 Cuts at: 4498 5110 5315 6057 6142 7213 1391 Size: 612 205 742 85 1071 2174 Fragments arranged by size: 2174 1231 1071 742 612 605 459 399 205 183 108 85 70 52 BspEI T'CCGG_A Cuts at: 7659 7659 Size: 7996 BspHI T'CATG_A Cuts at: 5885 7615 5885 Size: 1730 6266 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4135 4159 5351 844 Size: 3291 24 1192 3489 Fragments arranged by size: 3489 3291 1192 24 BsrBI CCG'CTC Cuts at: 1394 1823 2444 5883 1394 Size: 429 621 3439 3507 Fragments arranged by size: 3507 3439 621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6491 6665 65 Size: 1183 5243 174 1396 Fragments arranged by size: 5243 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2236 6640 6827 1700 Size: 110 426 4404 187 2869 Fragments arranged by size: 4404 2869 426 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7996 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7996 BssSI C'ACGA_G Cuts at: 6056 7068 6056 Size: 1012 6984 BstAPI GCAn_nnn'nTGC Cuts at: 794 2390 794 Size: 1596 6400 BstBI TT'CG_AA Cuts at: 6800 6800 Size: 7996 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7996 BstXI CCAn_nnnn'nTGG Cuts at: 4171 4219 4267 4315 4363 4171 Size: 48 48 48 48 7804 Fragments arranged by size: 7804 48 48 48 48 BstYI r'GATC_y Cuts at: 1972 2419 3815 4579 5326 5693 6078 6095 7547 Size: 447 1396 764 747 367 385 17 1452 Cuts at: 7547 7633 7645 7991 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1396 764 747 447 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2830 2830 Size: 7996 BtgI C'CryG_G Cuts at: 513 1065 2004 3161 3903 4011 4074 4119 4212 Size: 552 939 1157 742 108 63 45 93 Cuts at: 4212 4260 4356 513 Size: 48 96 4153 Fragments arranged by size: 4153 1157 939 742 552 108 96 93 63 48 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7257 Fragments arranged by size: 7257 683 56 BtsI GCAGTG_nn' Cuts at: 2065 2616 4601 4962 5512 6306 6326 2065 Size: 551 1985 361 550 794 20 3735 Fragments arranged by size: 3735 1985 794 551 550 361 20 ClaI AT'CG_AT Cuts at: 1554 2683 1554 Size: 1129 6867 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7961 DraI TTT'AAA Cuts at: 3001 4806 5300 3001 Size: 1805 494 5697 Fragments arranged by size: 5697 1805 494 DraIII CAC_nnn'GTG Cuts at: 3101 3101 Size: 7996 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7003 809 Size: 507 5687 1802 Fragments arranged by size: 5687 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2127 5111 Size: 54 1425 50 275 18 177 120 2984 Cuts at: 5111 5443 6332 7961 8 Size: 332 889 1629 43 Fragments arranged by size: 2984 1629 1425 889 332 275 177 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5443 1537 Size: 275 3631 4090 Fragments arranged by size: 4090 3631 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2280 2565 2617 2619 4620 4689 4698 5926 Size: 927 285 52 2 2001 69 9 1228 Cuts at: 5926 1353 Size: 3423 Fragments arranged by size: 3423 2001 1228 927 285 69 52 9 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6583 6957 7103 387 Size: 1064 138 4994 374 146 1280 Fragments arranged by size: 4994 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2463 2463 Size: 7996 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2379 2460 2535 2812 4670 4917 Size: 393 423 324 81 75 277 1858 247 Cuts at: 4917 6059 6656 7443 7711 1239 Size: 1142 597 787 268 1524 Fragments arranged by size: 1858 1524 1142 787 597 423 393 324 277 268 247 81 75 EcoO109I rG'GnC_Cy Cuts at: 2644 2645 3845 3875 3983 4109 2644 Size: 1 1200 30 108 126 6531 Fragments arranged by size: 6531 1200 126 108 30 1 EcoRI G'AATT_C Cuts at: 5305 5683 5305 Size: 378 7618 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4700 5170 4700 Size: 470 7526 FspI TGC'GCA Cuts at: 6502 6502 Size: 7996 HaeII r_GCGC'y Cuts at: 1678 7143 1678 Size: 5465 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2802 3602 3634 3800 Size: 32 135 32 1198 32 800 32 166 Cuts at: 3800 3817 3832 3849 5057 5089 6637 6669 6711 Size: 17 15 17 1208 32 1548 32 42 Cuts at: 6711 6743 1373 Size: 32 2626 Fragments arranged by size: 2626 1548 1208 1198 800 166 135 42 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3580 5340 5586 669 Size: 988 281 1642 1760 246 3079 Fragments arranged by size: 3079 1760 1642 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7996 HpaI GTT'AAC Cuts at: 5586 5586 Size: 7996 KpnI G_GTAC'C Cuts at: 5321 5321 Size: 7996 MfeI C'AATT_G Cuts at: 3172 5595 3172 Size: 2423 5573 MluI A'CGCG_T Cuts at: 7872 7872 Size: 7996 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7110 7294 1305 Size: 5805 184 2007 Fragments arranged by size: 5805 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5997 Fragments arranged by size: 5997 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2774 2917 2985 3001 4169 4217 Size: 648 906 702 143 68 16 1168 48 Cuts at: 4217 4265 4313 4361 5043 5954 6313 6472 518 Size: 48 48 48 682 911 359 159 2042 Fragments arranged by size: 2042 1168 911 906 702 682 648 359 159 143 68 48 48 48 48 16 MspA1I CmG'CkG Cuts at: 1606 2187 2664 5412 6089 7237 7482 1606 Size: 581 477 2748 677 1148 245 2120 Fragments arranged by size: 2748 2120 1148 677 581 477 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3903 4074 4119 4212 4260 4356 Size: 552 939 1899 171 45 93 48 96 Cuts at: 4356 513 Size: 4153 Fragments arranged by size: 4153 1899 939 552 171 96 93 48 45 NdeI CA'TA_TG Cuts at: 387 2970 387 Size: 2583 5413 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7996 NotI GC'GGCC_GC Cuts at: 5443 5443 Size: 7996 NspI r_CATG'y Cuts at: 1132 1229 2095 2838 2888 3143 3155 3914 4483 Size: 97 866 743 50 255 12 759 569 Cuts at: 4483 5074 5354 6899 1132 Size: 591 280 1545 2229 Fragments arranged by size: 2229 1545 866 759 743 591 569 280 255 97 50 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7996 PciI A'CATG_T Cuts at: 2091 2834 3139 3151 5070 6895 2091 Size: 743 305 12 1919 1825 3192 Fragments arranged by size: 3192 1919 1825 743 305 12 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 1108 Size: 912 7084 PmeI GTTT'AAAC Cuts at: 5300 5300 Size: 7996 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6065 6097 7578 7610 6065 Size: 32 1481 32 6451 Fragments arranged by size: 6451 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7996 PsiI TTA'TAA Cuts at: 5566 5566 Size: 7996 PspOMI G'GGCC_C Cuts at: 2644 3875 2644 Size: 1231 6765 PstI C_TGCA'G Cuts at: 830 2623 5348 830 Size: 1793 2725 3478 Fragments arranged by size: 3478 2725 1793 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7996 PvuII CAG'CTG Cuts at: 2187 2187 Size: 7996 SacI G_AGCT'C Cuts at: 2465 2465 Size: 7996 SalI G'TCGA_C Cuts at: 1936 5338 1936 Size: 3402 4594 SapI GCTCTTCn'nnn_ Cuts at: 2617 2617 Size: 7996 SbfI CC_TGCA'GG Cuts at: 5348 5348 Size: 7996 ScaI AGT'ACT Cuts at: 6244 7956 6244 Size: 1712 6284 SexAI A'CCwGG_T Cuts at: 2199 4723 2199 Size: 2524 5472 SfcI C'TryA_G Cuts at: 826 1045 2619 3215 5344 6479 7160 7351 826 Size: 219 1574 596 2129 1135 681 191 1471 Fragments arranged by size: 2129 1574 1471 1135 681 596 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7996 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7996 SmaI CCC'GGG Cuts at: 1872 5323 1872 Size: 3451 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2938 5283 6100 7001 7263 820 Size: 197 926 995 2345 817 901 262 1553 Fragments arranged by size: 2345 1553 995 926 901 817 262 197 SnaBI TAC'GTA Cuts at: 493 5787 493 Size: 5294 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7996 SphI G_CATG'C Cuts at: 1132 3914 5354 1132 Size: 2782 1440 3774 Fragments arranged by size: 3774 2782 1440 SspI AAT'ATT Cuts at: 5 52 3325 5920 5 Size: 47 3273 2595 2081 Fragments arranged by size: 3273 2595 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7996 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 2819 3903 4074 4119 4212 Size: 552 939 636 179 1084 171 45 93 Cuts at: 4212 4260 4356 5154 5435 513 Size: 48 96 798 281 3074 Fragments arranged by size: 3074 1084 939 798 636 552 281 179 171 96 93 48 45 SwaI ATTT'AAAT Cuts at: 3001 3001 Size: 7996 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 4022 6023 6040 6193 6378 6823 Size: 1255 447 1995 2001 17 153 185 445 Cuts at: 6823 325 Size: 1498 Fragments arranged by size: 2001 1995 1498 1255 447 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3737 4045 5262 Size: 274 80 33 51 1443 1760 308 1217 Cuts at: 5262 6242 7954 96 Size: 980 1712 138 Fragments arranged by size: 1760 1712 1443 1217 980 308 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7956 7988 7956 Size: 32 7964 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7996 XbaI T'CTAG_A Cuts at: 5332 5332 Size: 7996 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2122 2723 3593 4969 2017 Size: 105 601 870 1376 5044 Fragments arranged by size: 5044 1376 870 601 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7996 XmaI C'CCGG_G Cuts at: 1870 5321 1870 Size: 3451 4545 XmnI GAAnn'nnTTC Cuts at: 3529 6125 3529 Size: 2596 5400 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5804 276 Size: 53 83 186 936 4270 2468 Fragments arranged by size: 4270 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI