Restriction map for
pFN21AB5099
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
FbaI FseI HaeII Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeII MluI MluNI MroI
MscI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 45 CGCG CG^CG
AocI 2037 CCTGAGG CC^T[ACGT]AGG
ApaI 2198 GGGCCC GGGCC^C
AspI 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2047 TGCGCA TGC^GCA
BanII 2198 GGGCCC G[GA]GC[CT]^C
BciVI 1384 GGATAC GTATCC(6/5)
BpmI 437 CTCCAG CTGGAG(16/14)
BsaBI 1849 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1583 GAGGAG GAGGAG(10/8)
BsrFI 569 ACCGGC [GA]^CCGG[CT]
BstUI 45 CGCG CG^CG
Bsu36I 2037 CCTGAGG CC^T[ACGT]AGG
CfoI 2048 GCGC GCG^C
Cfr10I 569 ACCGGC [GA]^CCGG[CT]
Eco81I 2037 CCTGAGG CC^T[ACGT]AGG
EcoRI 414 GAATTC G^AATTC
EcoRV 625 GATATC GAT^ATC
EcoT14I 720 CCAAGG C^C[AT][AT]GG
FspI 2047 TGCGCA TGC^GCA
HapII 570 CCGG C^CGG
HgaI 46 GCGTC GACGC(5/10)
HhaI 2048 GCGC GCG^C
HinP1I 2048 GCGC G^CGC
HpaII 570 CCGG C^CGG
MamI 1849 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1565 CAATTG C^AATTG
MspA1I 1811 CAGCTG C[AC]G^C[GT]G
MspI 570 CCGG C^CGG
MunI 1565 CAATTG C^AATTG
MvnI 45 CGCG CG^CG
NheI 33 GCTAGC G^CTAGC
PflFI 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 698 CTGCAG CTGCA^G
PvuII 1811 CAGCTG CAG^CTG
SfaNI 1344 GATGC GCATC(5/9)
SfcI 698 CTGCAG C^T[GA][CT]AG
StyI 720 CCAAGG C^C[AT][AT]GG
Tth111I 886 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 1069 CTTAAG C^TTAAG
2191 CTTAAG
AspHI 259 GAGCAC G[AT]GC[AT]^C
2127 GAGCAC
BfrI 1069 CTTAAG C^TTAAG
2191 CTTAAG
BsgI 9 GTGCAG GTGCAG(16/14)
366 GTGCAG
BsiHKAI 259 GAGCAC G[AT]GC[AT]^C
2127 GAGCAC
BsmI 318 GAATGC GAATGC(1/-1)
2226 GAATGC
BstYI 141 AGATCC [GA]^GATC[CT]
1854 AGATCC
DraI 1321 TTTAAA TTT^AAA
1545 TTTAAA
EcoT22I 182 ATGCAT ATGCA^T
1777 ATGCAT
FauI 43 CCCGC CCCGC(4/6)
1902 GCGGG
HindIII 392 AAGCTT A^AGCTT
743 AAGCTT
MflI 141 AGATCC [GA]^GATC[CT]
1854 AGATCC
MslI 424 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
763 CATTACTGTG
NlaIV 2197 GGGGCC GG[ACGT]^[ACGT]CC
2198 GGGCCC
NsiI 182 ATGCAT ATGCA^T
1777 ATGCAT
PpuMI 2041 AGGACCT [GA]G^G[AT]CC[CT]
2075 AGGACCT
SspI 1205 AATATT AAT^ATT
1316 AATATT
TaqI 1756 TCGA T^CGA
1797 TCGA
TthHB8I 1756 TCGA T^CGA
1797 TCGA
XhoII 141 AGATCC [GA]^GATC[CT]
1854 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII PleI RcaI RsaI
Sau3AI Sau96I ScrFI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI