Restriction map for pFN21AB5099
 (Circular) (Six-base) MAPSORT of: pFN21AB5099.seq  Check: 6939  from: 1  to: 7015



pFN21AB5099  7015 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:35  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4825     278 
   Size:         53      83     186     936    3289    2468

  Fragments arranged by size: 

                3289    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4358    1937 
   Size:       2421    4594
 
Acc65I G'GTAC_C

Cuts at:   4336    4336 
   Size:       7015
 
AclI AA'CG_TT

Cuts at:   1563    4314    5142    5515    1563 
   Size:       2751     828     373    3063

  Fragments arranged by size: 

                3063    2751     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2645    3429    3678    3686    4286    5078    6462    6730 
   Size:       1013     784     249       8     600     792    1384     268

Cuts at:   6730    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384    1013     792     784     600     268     249
                   8
 
AflII C'TTAA_G

Cuts at:    820    1017    3074    4196     820 
   Size:        197    2057    1122    3639

  Fragments arranged by size: 

                3639    2057    1122     197
 
AflIII A'CryG_T

Cuts at:   1203    5914    6891    1203 
   Size:       4711     977    1327

  Fragments arranged by size: 

                4711    1327     977
 
AgeI A'CCGG_T

Cuts at:   5846    5846 
   Size:       7015
 
AhdI GACnn_n'nnGTC

Cuts at:   5744    5744 
   Size:       7015
 
AleI CACnn'nnGTG

Cuts at:   1166    2433    1166 
   Size:       1267    5748
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7015
 
AlwNI CAG_nnn'CTG

Cuts at:   4758    6330    4758 
   Size:       1572    5443
 
ApaI G_GGCC'C

Cuts at:   4207    4207 
   Size:       7015
 
ApaLI G'TGCA_C

Cuts at:   5072    6228    5072 
   Size:       1156    5859
 
ApoI r'AATT_y

Cuts at:   1493    2180    2419    2645    2665    3010    3224    3546    4324 
   Size:        687     239     226      20     345     214     322     778

Cuts at:   4324    4550    4702    6811    1493 
   Size:        226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     778     687     345     322     239     226
                 226     214     152      20
 
AseI AT'TA_AT

Cuts at:    160    1032    5569     160 
   Size:        872    4537    1606

  Fragments arranged by size: 

                4537    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7015
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4340    1870 
   Size:         73    2397    4545

  Fragments arranged by size: 

                4545    2397      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2596    2629    2596 
   Size:         33    6982
 
BamHI G'GATC_C

Cuts at:   4345    4712    4345 
   Size:        367    6648
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4336    5791     618 
   Size:        325     217     627    2549    1455    1842

  Fragments arranged by size: 

                2549    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    4207    4334    1391 
   Size:       2816     127    4072

  Fragments arranged by size: 

                4072    2816     127
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    6044
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5206    5240    1903 
   Size:         34    3269      34    3678

  Fragments arranged by size: 

                3678    3269      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3383    4907    6117    3383 
   Size:       1524    1210    4281

  Fragments arranged by size: 

                4281    1524    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7015
 
BfrBI ATG'CAT

Cuts at:   2189    3784    2189 
   Size:       1595    5420
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5626     136 
   Size:        107     122      71     631     605    3954    1525

  Fragments arranged by size: 

                3954    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   7010    7010 
   Size:       7015
 
BlpI GC'TnA_GC

Cuts at:   4432    4432 
   Size:       7015
 
Bme1580I G_kGCm'C

Cuts at:   1790    4207    5076    6232    1790 
   Size:       2417     869    1156    2573

  Fragments arranged by size: 

                2573    2417    1156     869
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7015
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2145    4746    5704 
   Size:        416      53      83     399      24     728    2601     958

Cuts at:   5704     442 
   Size:       1753

  Fragments arranged by size: 

                2601    1753     958     728     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    2042    1056 
   Size:        986    6029
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2427    5675    1239 
   Size:       1188    3248    2579

  Fragments arranged by size: 

                3248    2579    1188
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2325    3491    5140    6005    6303    2325 
   Size:       1166    1649     865     298    3037

  Fragments arranged by size: 

                3037    1649    1166     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5678     882 
   Size:        617    4179    2219

  Fragments arranged by size: 

                4179    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4806     493 
   Size:        711    3602    2702

  Fragments arranged by size: 

                3602    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3858    7009    3858 
   Size:       3151    3864
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4822    5204    5839     275 
   Size:         53      83     186     936    3289     382     635    1451

  Fragments arranged by size: 

                3289    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5448    5846    6120    6267    6678    5448 
   Size:        398     274     147     411    5785

  Fragments arranged by size: 

                5785     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6790

  Fragments arranged by size: 

                6790     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3603    1186 
   Size:       2417    4598
 
BseYI C'CCAG_C

Cuts at:   3725    4158    6218    6714    3725 
   Size:        433    2060     496    4026

  Fragments arranged by size: 

                4026    2060     496     433
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2035    2392    1163 
   Size:        573     299     357    5786

  Fragments arranged by size: 

                5786     573     357     299
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4465    5226    6254    1540 
   Size:        275     186    2464     761    1028    2301

  Fragments arranged by size: 

                2464    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2268    4136    5076    5161    6232    2268 
   Size:       1868     940      85    1071    3051

  Fragments arranged by size: 

                3051    1868    1071     940      85
 
BsmI GAATG_Cn'

Cuts at:   2329    4237    4526    4619    2329 
   Size:       1908     289      93    4725

  Fragments arranged by size: 

                4725    1908     289      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2268    4136    4207    4334    5076    5161    6232 
   Size:        399     478    1868      71     127     742      85    1071

Cuts at:   6232    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1868    1071     742     478     399     127      85
                  71
 
BspEI T'CCGG_A

Cuts at:   6678    6678 
   Size:       7015
 
BspHI T'CATG_A

Cuts at:   2556    2634    3850    4904    6634    2556 
   Size:         78    1216    1054    1730    2937

  Fragments arranged by size: 

                2937    1730    1216    1054      78
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2880    2920    3703    4058    4153    4370     844 
   Size:       2036      40     783     355      95     217    3489

  Fragments arranged by size: 

                3489    2036     783     355     217      95      40
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4902    1394 
   Size:        429    3079    3507

  Fragments arranged by size: 

                3507    3079     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3544    3842    3902    4239    5510    5684      65 
   Size:       1183    2296     298      60     337    1271     174    1396

  Fragments arranged by size: 

                2296    1396    1271    1183     337     298     174      60
            
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2574    5659    5846    1700 
   Size:        110     764    3085     187    2869

  Fragments arranged by size: 

                3085    2869     764     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7015
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7015
 
BssSI C'ACGA_G

Cuts at:   5075    6087    5075 
   Size:       1012    6003
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       7015
 
BstBI TT'CG_AA

Cuts at:   5819    5819 
   Size:       7015
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7015
 
BstYI r'GATC_y

Cuts at:   1972    2146    3859    4345    4712    5097    5114    6566    6652 
   Size:        174    1713     486     367     385      17    1452      86

Cuts at:   6652    6664    7010    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1713    1452     486     385     367     346     174
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   4043    4043 
   Size:       7015
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6463
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6276

  Fragments arranged by size: 

                6276     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2268    2700    2784    4531    5325    5345    2268 
   Size:        432      84    1747     794      20    3938

  Fragments arranged by size: 

                3938    1747     794     432      84      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7015
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6980
 
DraI TTT'AAA

Cuts at:   3328    3552    4319    3328 
   Size:        224     767    6024

  Fragments arranged by size: 

                6024     767     224
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6022     809 
   Size:        507    4706    1802

  Fragments arranged by size: 

                4706    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4462    5351    6980 
   Size:         54    1425      50     275      18    2632     889    1629

Cuts at:   6980       8 
   Size:         43

  Fragments arranged by size: 

                2632    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4462    1537 
   Size:        275    2650    4090

  Fragments arranged by size: 

                4090    2650     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4945    1353 
   Size:       3592    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3182    5602    5976    6122     387 
   Size:       1064     138    1593    2420     374     146    1280

  Fragments arranged by size: 

                2420    1593    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2427    2645    3429    3678    3686    4286    5078 
   Size:        393     795     218     784     249       8     600     792

Cuts at:   5078    5675    6462    6730    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     795     792     787     784     600     597     393
                 268     249     218       8
 
EcoO109I rG'GnC_Cy

Cuts at:   4047    4081    4203    4204    4047 
   Size:         34     122       1    6858

  Fragments arranged by size: 

                6858     122      34       1
 
EcoRI G'AATT_C

Cuts at:   2419    4324    4702    2419 
   Size:       1905     378    4732

  Fragments arranged by size: 

                4732    1905     378
 
EcoRV GAT'ATC

Cuts at:   2632    2632 
   Size:       7015
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3022    3022 
   Size:       7015
 
FspI TGC'GCA

Cuts at:   4054    5521    4054 
   Size:       1467    5548
 
HaeII r_GCGC'y

Cuts at:   1678    6162    1678 
   Size:       4484    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2964    2996    5656    5688    5730 
   Size:         32     135      32    1392      32    2660      32      42

Cuts at:   5730    5762    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2660    2626    1392     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4359    4605     669 
   Size:        988     281    2421     246    3079

  Fragments arranged by size: 

                3079    2421     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2397    2748     748 
   Size:       1649     351    5015

  Fragments arranged by size: 

                5015    1649     351
 
HpaI GTT'AAC

Cuts at:   4605    4605 
   Size:       7015
 
KpnI G_GTAC'C

Cuts at:   4340    4340 
   Size:       7015
 
MfeI C'AATT_G

Cuts at:   3570    4614    3570 
   Size:       1044    5971
 
MluI A'CGCG_T

Cuts at:   6891    6891 
   Size:       7015
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3774    3887    6129    6313    1305 
   Size:       2469     113    2242     184    2007

  Fragments arranged by size: 

                2469    2242    2007     184     113
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5193

  Fragments arranged by size: 

                5193    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2433    2772    4973    5332    5491     518 
   Size:        648    1267     339    2201     359     159    2042

  Fragments arranged by size: 

                2201    2042    1267     648     359     339     159
 
MspA1I CmG'CkG

Cuts at:   1606    3818    4431    5108    6256    6501    1606 
   Size:       2212     613     677    1148     245    2120

  Fragments arranged by size: 

                2212    2120    1148     677     613     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6463
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7015
 
NheI G'CTAG_C

Cuts at:   1052    2038    1052 
   Size:        986    6029
 
NotI GC'GGCC_GC

Cuts at:   4462    4462 
   Size:       7015
 
NsiI A_TGCA'T

Cuts at:   2191    3786    2191 
   Size:       1595    5420
 
NspI r_CATG'y

Cuts at:   1132    1229    4373    5918    1132 
   Size:         97    3144    1545    2229

  Fragments arranged by size: 

                3144    2229    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7015
 
PciI A'CATG_T

Cuts at:   5914    5914 
   Size:       7015
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       7015
 
PfoI T'CCnGG_A

Cuts at:   2443    2443 
   Size:       7015
 
PmeI GTTT'AAAC

Cuts at:   4319    4319 
   Size:       7015
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5084    5116    6597    6629    5084 
   Size:         32    1481      32    5470

  Fragments arranged by size: 

                5470    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   4047    4081    4047 
   Size:         34    6981
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7015
 
PsiI TTA'TAA

Cuts at:   3336    4585    3336 
   Size:       1249    5766
 
PspOMI G'GGCC_C

Cuts at:   4203    4203 
   Size:       7015
 
PstI C_TGCA'G

Cuts at:    830    2707    4367     830 
   Size:       1877    1660    3478

  Fragments arranged by size: 

                3478    1877    1660
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7015
 
PvuII CAG'CTG

Cuts at:   3818    3818 
   Size:       7015
 
SalI G'TCGA_C

Cuts at:   1936    4357    1936 
   Size:       2421    4594
 
SbfI CC_TGCA'GG

Cuts at:   4367    4367 
   Size:       7015
 
ScaI AGT'ACT

Cuts at:   5263    6975    5263 
   Size:       1712    5303
 
SfcI C'TryA_G

Cuts at:    826    1045    2703    4363    5498    6179    6370     826 
   Size:        219    1658    1660    1135     681     191    1471

  Fragments arranged by size: 

                1660    1658    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7015
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7015
 
SmaI CCC'GGG

Cuts at:   1872    4342    1872 
   Size:       2470    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2340    3074    3470    4196    5119    6020 
   Size:        197     926     397     734     396     726     923     901

Cuts at:   6020    6282     820 
   Size:        262    1553

  Fragments arranged by size: 

                1553     926     923     901     734     726     397     396
                 262     197
 
SnaBI TAC'GTA

Cuts at:    493    4806     493 
   Size:       4313    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7015
 
SphI G_CATG'C

Cuts at:   1132    4373    1132 
   Size:       3241    3774
 
SspI AAT'ATT

Cuts at:      5      52    3212    3323    4939       5 
   Size:         47    3160     111    1616    2081

  Fragments arranged by size: 

                3160    2081    1616     111      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       7015
 
StyI C'CwwG_G

Cuts at:    513    1065    2725    4454     513 
   Size:        552    1660    1729    3074

  Fragments arranged by size: 

                3074    1729    1660     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    5042    5059    5212    5397    5842     325 
   Size:       1255    3462      17     153     185     445    1498

  Fragments arranged by size: 

                3462    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2336    3287    3943 
   Size:        274      80      33      51    1443     359     951     656

Cuts at:   3943    3985    5261    6973      96 
   Size:         42    1276    1712     138

  Fragments arranged by size: 

                1712    1443    1276     951     656     359     274     138
                  80      51      42      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6975    7007    6975 
   Size:         32    6983
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2894    1316 
   Size:       1578    5437
 
XbaI T'CTAG_A

Cuts at:   4351    4351 
   Size:       7015
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7015
 
XmaI C'CCGG_G

Cuts at:   1870    4340    1870 
   Size:       2470    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2400    3138    3460    5144    2400 
   Size:        738     322    1684    4271

  Fragments arranged by size: 

                4271    1684     738     322
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4823     276 
   Size:         53      83     186     936    3289    2468

  Fragments arranged by size: 

                3289    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SalI
     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BbeI    BbvCI     BplI
   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII  EcoICRI    EcoNI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI
     PmlI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI
    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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