(Circular) (Six-base) MAPSORT of: pFN21AB5099.seq Check: 6939 from: 1 to: 7015 pFN21AB5099 7015 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4825 278 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4358 1937 Size: 2421 4594 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 4314 5142 5515 1563 Size: 2751 828 373 3063 Fragments arranged by size: 3063 2751 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 3429 3678 3686 4286 5078 6462 6730 Size: 1013 784 249 8 600 792 1384 268 Cuts at: 6730 1632 Size: 1917 Fragments arranged by size: 1917 1384 1013 792 784 600 268 249 8 AflII C'TTAA_G Cuts at: 820 1017 3074 4196 820 Size: 197 2057 1122 3639 Fragments arranged by size: 3639 2057 1122 197 AflIII A'CryG_T Cuts at: 1203 5914 6891 1203 Size: 4711 977 1327 Fragments arranged by size: 4711 1327 977 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 2433 1166 Size: 1267 5748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7015 AlwNI CAG_nnn'CTG Cuts at: 4758 6330 4758 Size: 1572 5443 ApaI G_GGCC'C Cuts at: 4207 4207 Size: 7015 ApaLI G'TGCA_C Cuts at: 5072 6228 5072 Size: 1156 5859 ApoI r'AATT_y Cuts at: 1493 2180 2419 2645 2665 3010 3224 3546 4324 Size: 687 239 226 20 345 214 322 778 Cuts at: 4324 4550 4702 6811 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 778 687 345 322 239 226 226 214 152 20 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 4340 1870 Size: 73 2397 4545 Fragments arranged by size: 4545 2397 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2596 2629 2596 Size: 33 6982 BamHI G'GATC_C Cuts at: 4345 4712 4345 Size: 367 6648 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4336 5791 618 Size: 325 217 627 2549 1455 1842 Fragments arranged by size: 2549 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4207 4334 1391 Size: 2816 127 4072 Fragments arranged by size: 4072 2816 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6044 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5206 5240 1903 Size: 34 3269 34 3678 Fragments arranged by size: 3678 3269 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3383 4907 6117 3383 Size: 1524 1210 4281 Fragments arranged by size: 4281 1524 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BfrBI ATG'CAT Cuts at: 2189 3784 2189 Size: 1595 5420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5626 136 Size: 107 122 71 631 605 3954 1525 Fragments arranged by size: 3954 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7010 7010 Size: 7015 BlpI GC'TnA_GC Cuts at: 4432 4432 Size: 7015 Bme1580I G_kGCm'C Cuts at: 1790 4207 5076 6232 1790 Size: 2417 869 1156 2573 Fragments arranged by size: 2573 2417 1156 869 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 4746 5704 Size: 416 53 83 399 24 728 2601 958 Cuts at: 5704 442 Size: 1753 Fragments arranged by size: 2601 1753 958 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2042 1056 Size: 986 6029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 5675 1239 Size: 1188 3248 2579 Fragments arranged by size: 3248 2579 1188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2325 3491 5140 6005 6303 2325 Size: 1166 1649 865 298 3037 Fragments arranged by size: 3037 1649 1166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5678 882 Size: 617 4179 2219 Fragments arranged by size: 4179 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4806 493 Size: 711 3602 2702 Fragments arranged by size: 3602 2702 711 BsaBI GATnn'nnATC Cuts at: 3858 7009 3858 Size: 3151 3864 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4822 5204 5839 275 Size: 53 83 186 936 3289 382 635 1451 Fragments arranged by size: 3289 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5448 5846 6120 6267 6678 5448 Size: 398 274 147 411 5785 Fragments arranged by size: 5785 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6790 Fragments arranged by size: 6790 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3603 1186 Size: 2417 4598 BseYI C'CCAG_C Cuts at: 3725 4158 6218 6714 3725 Size: 433 2060 496 4026 Fragments arranged by size: 4026 2060 496 433 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2035 2392 1163 Size: 573 299 357 5786 Fragments arranged by size: 5786 573 357 299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4465 5226 6254 1540 Size: 275 186 2464 761 1028 2301 Fragments arranged by size: 2464 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 4136 5076 5161 6232 2268 Size: 1868 940 85 1071 3051 Fragments arranged by size: 3051 1868 1071 940 85 BsmI GAATG_Cn' Cuts at: 2329 4237 4526 4619 2329 Size: 1908 289 93 4725 Fragments arranged by size: 4725 1908 289 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 4136 4207 4334 5076 5161 6232 Size: 399 478 1868 71 127 742 85 1071 Cuts at: 6232 1391 Size: 2174 Fragments arranged by size: 2174 1868 1071 742 478 399 127 85 71 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 2556 2634 3850 4904 6634 2556 Size: 78 1216 1054 1730 2937 Fragments arranged by size: 2937 1730 1216 1054 78 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2880 2920 3703 4058 4153 4370 844 Size: 2036 40 783 355 95 217 3489 Fragments arranged by size: 3489 2036 783 355 217 95 40 BsrBI CCG'CTC Cuts at: 1394 1823 4902 1394 Size: 429 3079 3507 Fragments arranged by size: 3507 3079 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3544 3842 3902 4239 5510 5684 65 Size: 1183 2296 298 60 337 1271 174 1396 Fragments arranged by size: 2296 1396 1271 1183 337 298 174 60 BsrFI r'CCGG_y Cuts at: 1700 1810 2574 5659 5846 1700 Size: 110 764 3085 187 2869 Fragments arranged by size: 3085 2869 764 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 5075 6087 5075 Size: 1012 6003 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7015 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7015 BstYI r'GATC_y Cuts at: 1972 2146 3859 4345 4712 5097 5114 6566 6652 Size: 174 1713 486 367 385 17 1452 86 Cuts at: 6652 6664 7010 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1713 1452 486 385 367 346 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4043 4043 Size: 7015 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6463 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6276 Fragments arranged by size: 6276 683 56 BtsI GCAGTG_nn' Cuts at: 2268 2700 2784 4531 5325 5345 2268 Size: 432 84 1747 794 20 3938 Fragments arranged by size: 3938 1747 794 432 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 3328 3552 4319 3328 Size: 224 767 6024 Fragments arranged by size: 6024 767 224 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6022 809 Size: 507 4706 1802 Fragments arranged by size: 4706 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4462 5351 6980 Size: 54 1425 50 275 18 2632 889 1629 Cuts at: 6980 8 Size: 43 Fragments arranged by size: 2632 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4462 1537 Size: 275 2650 4090 Fragments arranged by size: 4090 2650 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4945 1353 Size: 3592 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3182 5602 5976 6122 387 Size: 1064 138 1593 2420 374 146 1280 Fragments arranged by size: 2420 1593 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2427 2645 3429 3678 3686 4286 5078 Size: 393 795 218 784 249 8 600 792 Cuts at: 5078 5675 6462 6730 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 795 792 787 784 600 597 393 268 249 218 8 EcoO109I rG'GnC_Cy Cuts at: 4047 4081 4203 4204 4047 Size: 34 122 1 6858 Fragments arranged by size: 6858 122 34 1 EcoRI G'AATT_C Cuts at: 2419 4324 4702 2419 Size: 1905 378 4732 Fragments arranged by size: 4732 1905 378 EcoRV GAT'ATC Cuts at: 2632 2632 Size: 7015 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3022 3022 Size: 7015 FspI TGC'GCA Cuts at: 4054 5521 4054 Size: 1467 5548 HaeII r_GCGC'y Cuts at: 1678 6162 1678 Size: 4484 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2964 2996 5656 5688 5730 Size: 32 135 32 1392 32 2660 32 42 Cuts at: 5730 5762 1373 Size: 32 2626 Fragments arranged by size: 2660 2626 1392 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4359 4605 669 Size: 988 281 2421 246 3079 Fragments arranged by size: 3079 2421 988 281 246 HindIII A'AGCT_T Cuts at: 748 2397 2748 748 Size: 1649 351 5015 Fragments arranged by size: 5015 1649 351 HpaI GTT'AAC Cuts at: 4605 4605 Size: 7015 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 3570 4614 3570 Size: 1044 5971 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3774 3887 6129 6313 1305 Size: 2469 113 2242 184 2007 Fragments arranged by size: 2469 2242 2007 184 113 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5193 Fragments arranged by size: 5193 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2433 2772 4973 5332 5491 518 Size: 648 1267 339 2201 359 159 2042 Fragments arranged by size: 2201 2042 1267 648 359 339 159 MspA1I CmG'CkG Cuts at: 1606 3818 4431 5108 6256 6501 1606 Size: 2212 613 677 1148 245 2120 Fragments arranged by size: 2212 2120 1148 677 613 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6463 NdeI CA'TA_TG Cuts at: 387 387 Size: 7015 NheI G'CTAG_C Cuts at: 1052 2038 1052 Size: 986 6029 NotI GC'GGCC_GC Cuts at: 4462 4462 Size: 7015 NsiI A_TGCA'T Cuts at: 2191 3786 2191 Size: 1595 5420 NspI r_CATG'y Cuts at: 1132 1229 4373 5918 1132 Size: 97 3144 1545 2229 Fragments arranged by size: 3144 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7015 PciI A'CATG_T Cuts at: 5914 5914 Size: 7015 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7015 PfoI T'CCnGG_A Cuts at: 2443 2443 Size: 7015 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5084 5116 6597 6629 5084 Size: 32 1481 32 5470 Fragments arranged by size: 5470 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4047 4081 4047 Size: 34 6981 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 3336 4585 3336 Size: 1249 5766 PspOMI G'GGCC_C Cuts at: 4203 4203 Size: 7015 PstI C_TGCA'G Cuts at: 830 2707 4367 830 Size: 1877 1660 3478 Fragments arranged by size: 3478 1877 1660 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3818 3818 Size: 7015 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SbfI CC_TGCA'GG Cuts at: 4367 4367 Size: 7015 ScaI AGT'ACT Cuts at: 5263 6975 5263 Size: 1712 5303 SfcI C'TryA_G Cuts at: 826 1045 2703 4363 5498 6179 6370 826 Size: 219 1658 1660 1135 681 191 1471 Fragments arranged by size: 1660 1658 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 4342 1872 Size: 2470 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2340 3074 3470 4196 5119 6020 Size: 197 926 397 734 396 726 923 901 Cuts at: 6020 6282 820 Size: 262 1553 Fragments arranged by size: 1553 926 923 901 734 726 397 396 262 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7015 SphI G_CATG'C Cuts at: 1132 4373 1132 Size: 3241 3774 SspI AAT'ATT Cuts at: 5 52 3212 3323 4939 5 Size: 47 3160 111 1616 2081 Fragments arranged by size: 3160 2081 1616 111 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7015 StyI C'CwwG_G Cuts at: 513 1065 2725 4454 513 Size: 552 1660 1729 3074 Fragments arranged by size: 3074 1729 1660 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5042 5059 5212 5397 5842 325 Size: 1255 3462 17 153 185 445 1498 Fragments arranged by size: 3462 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2336 3287 3943 Size: 274 80 33 51 1443 359 951 656 Cuts at: 3943 3985 5261 6973 96 Size: 42 1276 1712 138 Fragments arranged by size: 1712 1443 1276 951 656 359 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6975 7007 6975 Size: 32 6983 Tth111I GACn'n_nGTC Cuts at: 1316 2894 1316 Size: 1578 5437 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 4340 1870 Size: 2470 4545 XmnI GAAnn'nnTTC Cuts at: 2400 3138 3460 5144 2400 Size: 738 322 1684 4271 Fragments arranged by size: 4271 1684 738 322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4823 276 Size: 53 83 186 936 3289 2468 Fragments arranged by size: 3289 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI