(Circular) (Six-base) MAPSORT of: pFN21AB5021.seq Check: 3938 from: 1 to: 6193 pFN21AB5021 6193 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2853 3536 1937 Size: 916 683 4594 Fragments arranged by size: 4594 916 683 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2234 2412 3015 3459 4256 5640 5908 Size: 499 103 178 603 444 797 1384 268 Cuts at: 5908 1632 Size: 1917 Fragments arranged by size: 1917 1384 797 603 499 444 268 178 103 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2899 5092 6069 1203 Size: 1696 2193 977 1327 Fragments arranged by size: 2193 1696 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 2903 4922 2903 Size: 2019 4174 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2291 2561 3240 3502 3728 3880 5989 1493 Size: 798 270 679 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 679 270 262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2966 2999 2966 Size: 33 6160 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3039 3218 3514 4969 618 Size: 325 217 627 1252 179 296 1455 1842 Fragments arranged by size: 1842 1455 1252 627 325 296 217 179 BanII G_rGCy'C Cuts at: 1391 3512 1391 Size: 2121 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 3234 3486 928 Size: 971 238 1097 252 3635 Fragments arranged by size: 3635 1097 971 252 238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4384 4418 1903 Size: 34 50 34 2363 34 3678 Fragments arranged by size: 3678 2363 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2325 4085 5295 2325 Size: 1760 1210 3223 Fragments arranged by size: 3223 1760 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BfrBI ATG'CAT Cuts at: 3446 3446 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 4254 5410 1790 Size: 2464 1156 2573 Fragments arranged by size: 2573 2464 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3924 4882 442 Size: 416 53 83 399 24 2507 958 1753 Fragments arranged by size: 2507 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4853 1239 Size: 3614 2579 Bpu10I CC'TnA_GC Cuts at: 2781 2781 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4856 882 Size: 617 3357 2219 Fragments arranged by size: 3357 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4000 4382 5017 275 Size: 53 83 186 936 2467 382 635 1451 Fragments arranged by size: 2467 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6193 BseYI C'CCAG_C Cuts at: 2060 3273 5396 5892 2060 Size: 1213 2123 496 2361 Fragments arranged by size: 2361 2123 1213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5620 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2646 2787 4254 4339 5410 2646 Size: 141 1467 85 1071 3429 Fragments arranged by size: 3429 1467 1071 141 85 BsmI GAATG_Cn' Cuts at: 2055 2348 3424 3446 3704 3797 2055 Size: 293 1076 22 258 93 4451 Fragments arranged by size: 4451 1076 293 258 93 22 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2646 2787 3512 4254 4339 5410 1391 Size: 399 856 141 725 742 85 1071 2174 Fragments arranged by size: 2174 1071 856 742 725 399 141 85 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4837 5024 1700 Size: 110 3027 187 2869 Fragments arranged by size: 3027 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3286 96 Size: 3190 3003 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 3443 794 Size: 2649 3544 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 3430 3430 Size: 6193 BstYI r'GATC_y Cuts at: 1972 2383 3523 3890 4275 4292 5744 5830 5842 Size: 411 1140 367 385 17 1452 86 12 Cuts at: 5842 6188 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1140 411 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2854 2854 Size: 6193 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4684 Fragments arranged by size: 4684 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 3709 4503 4523 3709 Size: 794 20 5379 Fragments arranged by size: 5379 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 2187 3497 2187 Size: 1310 4883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3640 4529 6158 Size: 54 1425 50 275 18 1810 889 1629 Cuts at: 6158 8 Size: 43 Fragments arranged by size: 1810 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 4123 1353 Size: 731 2039 3423 Fragments arranged by size: 3423 2039 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2131 2234 2412 3015 3459 4256 4853 Size: 393 499 103 178 603 444 797 597 Cuts at: 4853 5640 5908 1239 Size: 787 268 1524 Fragments arranged by size: 1524 797 787 603 597 499 444 393 268 178 103 EcoRI G'AATT_C Cuts at: 2291 3502 3880 2291 Size: 1211 378 4604 Fragments arranged by size: 4604 1211 378 EcoRV GAT'ATC Cuts at: 3340 3340 Size: 6193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3247 3251 3247 Size: 4 6189 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2889 2921 4834 4866 4908 Size: 32 135 32 1317 32 1913 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2626 1913 1317 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 2708 748 Size: 1960 4233 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2656 5307 5491 1305 Size: 1351 2651 184 2007 Fragments arranged by size: 2651 2007 1351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2337 4151 4510 4669 518 Size: 648 1171 1814 359 159 2042 Fragments arranged by size: 2042 1814 1171 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3273 3609 4286 5434 5679 1606 Size: 1667 336 677 1148 245 2120 Fragments arranged by size: 2120 1667 1148 677 336 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4684 Fragments arranged by size: 4684 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NsiI A_TGCA'T Cuts at: 3448 3448 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 2903 3551 5096 1132 Size: 97 1674 648 1545 2229 Fragments arranged by size: 2229 1674 1545 648 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 2899 5092 2899 Size: 2193 4000 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 2106 2178 2328 3763 2106 Size: 72 150 1435 4536 Fragments arranged by size: 4536 1435 150 72 PstI C_TGCA'G Cuts at: 830 3545 830 Size: 2715 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 PvuII CAG'CTG Cuts at: 3273 3273 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 3027 3411 3541 4676 5357 5548 826 Size: 219 1982 384 130 1135 681 191 1471 Fragments arranged by size: 1982 1471 1135 681 384 219 191 130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4297 5198 5460 820 Size: 197 926 2354 901 262 1553 Fragments arranged by size: 2354 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 2235 2878 4117 5 Size: 47 2183 643 1239 2081 Fragments arranged by size: 2183 2081 1239 643 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2022 3043 3222 3632 513 Size: 552 957 1021 179 410 3074 Fragments arranged by size: 3074 1021 957 552 410 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3286 4439 6151 Size: 274 80 33 51 1443 1309 1153 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 1712 1443 1309 1153 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 2295 2877 3231 3375 4322 2295 Size: 582 354 144 947 4166 Fragments arranged by size: 4166 947 582 354 144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI