Restriction map for
pFN21AB4967
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII Acc65I AccII AccIII AclI
AgeI AocI AscI Asp718I AspI
AviII AvrII BbeI BclI BlnI
BseAI BsiEI BsiWI BspDI BspEI
BsrFI BssHII BstUI Bsu36I Cfr10I
ClaI CpoI EagI EclXI Eco52I
Eco81I EcoRV FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfoI SgrAI SnaBI Sse8387I SspI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflII 724 CTTAAG C^TTAAG
AflIII 1853 ACATGT A^C[GA][CT]GT
AhdI 5916 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5207 AGCGCT AGC^GCT
ApaI 5758 GGGCCC GGGCC^C
AspEI 5916 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4711 CCCGGG C^[CT]CG[GA]G
BbrPI 5830 CACGTG CAC^GTG
BfrI 724 CTTAAG C^TTAAG
BglII 3767 AGATCT A^GATCT
BsaAI 5830 CACGTG [CT]AC^GT[GA]
BsaWI 3395 ACCGGA [AT]^CCGG[AT]
BsoBI 4711 CCCGGG C^[CT]CG[GA]G
BspLU11I 1853 ACATGT A^CATGT
BsrBI 1877 GAGCGG CCGCTC(-3/-3)
BssSI 3557 CTCGTG CACGAG(-5/-1)
BstBI 1736 TTCGAA TT^CGAA
BstEII 5712 GGTGACC G^GT[ACGT]ACC
BstPI 5712 GGTGACC G^GT[ACGT]ACC
Eam1105I 5916 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5207 AGCGCT AGC^GCT
EcoO65I 5712 GGTGACC G^GT[ACGT]ACC
NheI 7129 GCTAGC G^CTAGC
NspV 1736 TTCGAA TT^CGAA
PmaCI 5830 CACGTG CAC^GTG
PmlI 5830 CACGTG CAC^GTG
PshAI 4230 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2228 GAGCTC GAGCT^C
ScaI 3091 AGTACT AGT^ACT
SfiI 312 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1736 TTCGAA TT^CGAA
SmaI 4711 CCCGGG CCC^GGG
XbaI 3903 TCTAGA T^CTAGA
XmaCI 4711 CCCGGG C^CCGGG
XmaI 4711 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 72 GGCGTC G[GA]^CG[CT]C
5601 GACGCC
Alw44I 3546 GTGCAC G^TGCAC
3836 GTGCAC
ApaLI 3546 GTGCAC G^TGCAC
3836 GTGCAC
AseI 138 ATTAAT AT^TAAT
181 ATTAAT
AsnI 138 ATTAAT AT^TAAT
181 ATTAAT
BcgI 5957 CGACAGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2733 GCAGCAGCCTCG
BsaBI 178 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4840 GATCCTGATC
BsaHI 72 GGCGTC G[GA]^CG[CT]C
5601 GACGCC
BsmBI 5809 GAGACG CGTCTC(1/5)
6549 GAGACG
Bsp1407I 1216 TGTACA T^GTACA
3739 TGTACA
BspHI 572 TCATGA T^CATGA
4242 TCATGA
BsrGI 1216 TGTACA T^GTACA
3739 TGTACA
Bst1107I 3412 GTATAC GTA^TAC
4177 GTATAC
BstZ17I 3412 GTATAC GTA^TAC
4177 GTATAC
EcoT22I 3760 ATGCAT ATGCA^T
4126 ATGCAT
Hin1I 72 GGCGTC G[GA]^CG[CT]C
5601 GACGCC
MaeII 5831 ACGT A^CGT
7016 ACGT
MamI 178 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4840 GATCCTGATC
MfeI 584 CAATTG C^AATTG
5253 CAATTG
MunI 584 CAATTG C^AATTG
5253 CAATTG
NdeI 1829 CATATG CA^TATG
6847 CATATG
NsiI 3760 ATGCAT ATGCA^T
4126 ATGCAT
PshBI 138 ATTAAT AT^TAAT
181 ATTAAT
RcaI 572 TCATGA T^CATGA
4242 TCATGA
SpeI 418 ACTAGT A^CTAGT
2869 ACTAGT
SspBI 1216 TGTACA T^GTACA
3739 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI