(Circular) (Six-base) MAPSORT of: pFN21AB4967.seq Check: 8509 from: 1 to: 11947 pFN21AB4967 11947 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7019 7094 7780 8753 7019 Size: 75 686 973 10213 Fragments arranged by size: 10213 973 686 75 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9757 278 Size: 53 83 186 936 8221 2468 Fragments arranged by size: 8221 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5418 6183 7005 8773 9290 1937 Size: 3481 765 822 1768 517 4594 Fragments arranged by size: 4594 3481 1768 822 765 517 Acc65I G'GTAC_C Cuts at: 9268 9268 Size: 11947 AclI AA'CG_TT Cuts at: 1563 10074 10447 1563 Size: 8511 373 3063 Fragments arranged by size: 8511 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2022 2180 2657 4187 5085 6498 8783 10010 Size: 390 158 477 1530 898 1413 2285 1227 Cuts at: 10010 11394 11662 1632 Size: 1384 268 1917 Fragments arranged by size: 2285 1917 1530 1413 1384 1227 898 477 390 268 158 AfeI AGC'GCT Cuts at: 7214 7214 Size: 11947 AflII C'TTAA_G Cuts at: 820 1017 2729 820 Size: 197 1712 10038 Fragments arranged by size: 10038 1712 197 AflIII A'CryG_T Cuts at: 1203 3858 9244 10846 11823 1203 Size: 2655 5386 1602 977 1327 Fragments arranged by size: 5386 2655 1602 1327 977 AgeI A'CCGG_T Cuts at: 10778 10778 Size: 11947 AhdI GACnn_n'nnGTC Cuts at: 7926 10676 7926 Size: 2750 9197 AleI CACnn'nnGTG Cuts at: 1166 4886 6620 1166 Size: 3720 1734 6493 Fragments arranged by size: 6493 3720 1734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 7377 8286 1184 Size: 6193 909 4845 Fragments arranged by size: 6193 4845 909 AlwNI CAG_nnn'CTG Cuts at: 2888 4589 4772 5176 5527 7228 8138 9690 11262 Size: 1701 183 404 351 1701 910 1552 1572 Cuts at: 11262 2888 Size: 3573 Fragments arranged by size: 3573 1701 1701 1572 1552 910 404 351 183 ApaI G_GGCC'C Cuts at: 7767 7767 Size: 11947 ApaLI G'TGCA_C Cuts at: 5551 5841 10004 11160 5551 Size: 290 4163 1156 6338 Fragments arranged by size: 6338 4163 1156 290 ApoI r'AATT_y Cuts at: 1493 3616 3702 4492 4891 7842 9112 9211 9256 Size: 2123 86 790 399 2951 1270 99 45 Cuts at: 9256 9482 9634 11743 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2951 2123 2109 1697 1270 790 399 226 152 99 86 45 AseI AT'TA_AT Cuts at: 160 1032 2144 2187 10501 160 Size: 872 1112 43 8314 1606 Fragments arranged by size: 8314 1606 1112 872 43 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11947 AvaI C'yCGr_G Cuts at: 1870 1943 6716 9272 1870 Size: 73 4773 2556 4545 Fragments arranged by size: 4773 4545 2556 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5479 5512 7063 7096 5479 Size: 33 1551 33 10330 Fragments arranged by size: 10330 1551 33 33 BamHI G'GATC_C Cuts at: 3790 5209 5722 9277 9644 3790 Size: 1419 513 3555 367 6093 Fragments arranged by size: 6093 3555 1419 513 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5900 7338 7411 8765 9268 Size: 325 217 627 4113 1438 73 1354 503 Cuts at: 9268 10723 618 Size: 1455 1842 Fragments arranged by size: 4113 1842 1455 1438 1354 627 503 325 217 73 BanII G_rGCy'C Cuts at: 1391 2684 4237 4558 5291 5346 6946 7184 7767 Size: 1293 1553 321 733 55 1600 238 583 Cuts at: 7767 8492 8843 9266 1391 Size: 725 351 423 4072 Fragments arranged by size: 4072 1600 1553 1293 733 725 583 423 351 321 238 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3575 5019 5091 6778 928 Size: 971 1676 1444 72 1687 6097 Fragments arranged by size: 6097 1687 1676 1444 971 72 BbvCI CC'TCA_GC Cuts at: 5247 7629 8007 9003 5247 Size: 2382 378 996 8191 Fragments arranged by size: 8191 2382 996 378 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4727 4761 7951 7985 10138 10172 1903 Size: 34 2790 34 3190 34 2153 34 3678 Fragments arranged by size: 3678 3190 2790 2153 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5480 7217 8231 9839 11049 5480 Size: 1737 1014 1608 1210 6378 Fragments arranged by size: 6378 1737 1608 1210 1014 BclI T'GATC_A Cuts at: 1548 1548 Size: 11947 BfrBI ATG'CAT Cuts at: 5767 6133 5767 Size: 366 11581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2324 3156 Size: 107 122 71 631 605 403 249 832 Cuts at: 3156 4618 8329 10558 136 Size: 1462 3711 2229 1525 Fragments arranged by size: 3711 2229 1525 1462 832 631 605 403 249 122 107 71 BglII A'GATC_T Cuts at: 5772 11942 5772 Size: 6170 5777 BlpI GC'TnA_GC Cuts at: 4557 7746 8499 9364 4557 Size: 3189 753 865 7140 Fragments arranged by size: 7140 3189 865 753 Bme1580I G_kGCm'C Cuts at: 1790 2423 3657 5555 5845 7343 7767 10008 11164 Size: 633 1234 1898 290 1498 424 2241 1156 Cuts at: 11164 1790 Size: 2573 Fragments arranged by size: 2573 2241 1898 1498 1234 1156 633 424 290 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 11947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5104 5462 6940 Size: 416 53 83 399 24 3687 358 1478 Cuts at: 6940 7926 8866 9678 10636 442 Size: 986 940 812 958 1753 Fragments arranged by size: 3687 1753 1478 986 958 940 812 416 399 358 83 53 24 BmtI G_CTAG'C Cuts at: 1056 9138 1056 Size: 8082 3865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6166 6166 Size: 11947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2940 3000 3318 4251 4356 4416 5109 Size: 1565 136 60 318 933 105 60 693 Cuts at: 5109 6941 8008 8450 8962 10607 1239 Size: 1832 1067 442 512 1645 2579 Fragments arranged by size: 2579 1832 1645 1565 1067 933 693 512 442 318 136 105 60 60 Bpu10I CC'TnA_GC Cuts at: 3362 5247 7629 8007 9003 3362 Size: 1885 2382 378 996 6306 Fragments arranged by size: 6306 2382 1885 996 378 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4365 6250 7659 8572 10072 10937 11235 4365 Size: 1885 1409 913 1500 865 298 5077 Fragments arranged by size: 5077 1885 1500 1409 913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2822 4443 8187 10610 882 Size: 617 1323 1621 3744 2423 2219 Fragments arranged by size: 3744 2423 2219 1621 1323 617 BsaAI yAC'GTr Cuts at: 493 1204 7837 9738 493 Size: 711 6633 1901 2702 Fragments arranged by size: 6633 2702 1901 711 BsaBI GATnn'nnATC Cuts at: 2187 6849 11941 2187 Size: 4662 5092 2193 Fragments arranged by size: 5092 4662 2193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 7607 9754 10136 Size: 53 83 186 936 545 5529 2147 382 Cuts at: 10136 10771 275 Size: 635 1451 Fragments arranged by size: 5529 2147 1451 936 635 545 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 10380 10778 11052 11199 11610 5400 Size: 4980 398 274 147 411 5737 Fragments arranged by size: 5737 4980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3291 3321 7527 7557 1178 Size: 30 165 30 1888 30 4206 30 5568 Fragments arranged by size: 5568 4206 1888 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4188 5093 6190 7725 1186 Size: 3002 905 1097 1535 5408 Fragments arranged by size: 5408 3002 1535 1097 905 BseYI C'CCAG_C Cuts at: 2721 3386 4167 4464 4734 4827 5664 7611 8013 Size: 665 781 297 270 93 837 1947 402 Cuts at: 8013 8061 8186 8238 8442 8844 8934 8964 11150 Size: 48 125 52 204 402 90 30 2186 Cuts at: 11150 11646 2721 Size: 496 3022 Fragments arranged by size: 3022 2186 1947 837 781 665 496 402 402 297 270 204 125 93 90 52 48 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4698 4715 6962 7319 1163 Size: 573 2962 17 2247 357 5791 Fragments arranged by size: 5791 2962 2247 573 357 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 9397 10158 11186 1540 Size: 275 186 7396 761 1028 2301 Fragments arranged by size: 7396 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2986 3304 4237 4763 5555 5845 10008 10093 Size: 859 318 933 526 792 290 4163 85 Cuts at: 10093 11164 2127 Size: 1071 2910 Fragments arranged by size: 4163 2910 1071 933 859 792 526 318 290 85 BsmI GAATG_Cn' Cuts at: 2518 2904 3604 7025 7258 8252 9458 9551 2518 Size: 386 700 3421 233 994 1206 93 4914 Fragments arranged by size: 4914 3421 1206 994 700 386 233 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7808 8548 7808 Size: 740 11207 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2423 2684 2986 3304 3657 4237 Size: 399 337 296 261 302 318 353 580 Cuts at: 4237 4558 4763 5291 5346 5555 5845 6946 7184 Size: 321 205 528 55 209 290 1101 238 Cuts at: 7184 7343 7767 8492 8843 9266 10008 10093 11164 Size: 159 424 725 351 423 742 85 1071 Cuts at: 11164 1391 Size: 2174 Fragments arranged by size: 2174 1101 1071 742 725 580 528 424 423 399 353 351 337 321 318 302 296 290 261 238 209 205 159 85 55 BspEI T'CCGG_A Cuts at: 11610 11610 Size: 11947 BspHI T'CATG_A Cuts at: 2577 6247 9836 11566 2577 Size: 3670 3589 1730 2958 Fragments arranged by size: 3670 3589 2958 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2920 7019 7094 7780 8753 9302 844 Size: 2076 4099 75 686 973 549 3489 Fragments arranged by size: 4099 3489 2076 973 686 549 75 BsrBI CCG'CTC Cuts at: 1394 1823 3884 9834 1394 Size: 429 2061 5950 3507 Fragments arranged by size: 5950 3507 2061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2554 4902 6349 6613 6804 7021 7359 Size: 1183 1306 2348 1447 264 191 217 338 Cuts at: 7359 7797 10442 10616 65 Size: 438 2645 174 1396 Fragments arranged by size: 2645 2348 1447 1396 1306 1183 438 338 264 217 191 174 BsrFI r'CCGG_y Cuts at: 1700 1810 10591 10778 1700 Size: 110 8781 187 2869 Fragments arranged by size: 8781 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3221 5744 96 Size: 3125 2523 6299 Fragments arranged by size: 6299 3125 2523 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11947 BssSI C'ACGA_G Cuts at: 5562 10007 11019 5562 Size: 4445 1012 6490 Fragments arranged by size: 6490 4445 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3165 3182 3973 4721 7020 7456 8212 8569 Size: 2371 17 791 748 2299 436 756 357 Cuts at: 8569 8683 8695 8704 8854 794 Size: 114 12 9 150 3887 Fragments arranged by size: 3887 2371 2299 791 756 748 436 357 150 114 17 12 9 BstBI TT'CG_AA Cuts at: 3742 10751 3742 Size: 7009 4938 BstEII G'GTnAC_C Cuts at: 1184 7717 1184 Size: 6533 5414 BstXI CCAn_nnnn'nTGG Cuts at: 6541 8654 9079 9181 6541 Size: 2113 425 102 9307 Fragments arranged by size: 9307 2113 425 102 BstYI r'GATC_y Cuts at: 1972 3736 3790 3921 5209 5296 5625 5722 5772 Size: 1764 54 131 1288 87 329 97 50 Cuts at: 5772 8898 9277 9644 10029 10046 11498 11584 11596 Size: 3126 379 367 385 17 1452 86 12 Cuts at: 11596 11942 1972 Size: 346 1977 Fragments arranged by size: 3126 1977 1764 1452 1288 385 379 367 346 329 131 97 87 86 54 50 17 12 BstZ17I GTA'TAC Cuts at: 5419 6184 5419 Size: 765 11182 BtgI C'CryG_G Cuts at: 513 1065 2004 2490 4087 4210 5292 6000 6765 Size: 552 939 486 1597 123 1082 708 765 Cuts at: 6765 7887 7896 8475 8951 9078 9160 9174 513 Size: 1122 9 579 476 127 82 14 3286 Fragments arranged by size: 3286 1597 1122 1082 939 765 708 579 552 486 476 127 123 82 14 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2103 487 Size: 683 56 877 10331 Fragments arranged by size: 10331 877 683 56 BtsI GCAGTG_nn' Cuts at: 3098 3145 4099 7265 7387 7785 9463 10257 10277 Size: 47 954 3166 122 398 1678 794 20 Cuts at: 10277 3098 Size: 4768 Fragments arranged by size: 4768 3166 1678 954 794 398 122 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 11947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6015 6050 7644 7679 529 Size: 35 5451 35 1594 35 4797 Fragments arranged by size: 5451 4797 1594 35 35 35 DraI TTT'AAA Cuts at: 3804 5832 6466 9251 3804 Size: 2028 634 2785 6500 Fragments arranged by size: 6500 2785 2028 634 DraIII CAC_nnn'GTG Cuts at: 3182 6326 6960 7033 3182 Size: 3144 634 73 8096 Fragments arranged by size: 8096 3144 634 73 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3234 7001 8179 10954 809 Size: 507 1918 3767 1178 2775 1802 Fragments arranged by size: 3767 2775 1918 1802 1178 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2316 4150 Size: 54 1425 50 275 18 177 309 1834 Cuts at: 4150 7500 7582 8619 8954 9394 10283 11912 8 Size: 3350 82 1037 335 440 889 1629 43 Fragments arranged by size: 3350 1834 1629 1425 1037 889 440 335 309 275 177 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9394 1537 Size: 275 7582 4090 Fragments arranged by size: 7582 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2676 4206 4488 5037 5279 6573 6934 9877 Size: 1323 1530 282 549 242 1294 361 2943 Cuts at: 9877 1353 Size: 3423 Fragments arranged by size: 3423 2943 1530 1323 1294 549 361 282 242 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 6788 10534 10908 11054 387 Size: 1064 138 482 4717 3746 374 146 1280 Fragments arranged by size: 4717 3746 1280 1064 482 374 146 138 EcoICRI GAG'CTC Cuts at: 4235 4235 Size: 11947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2180 2657 2804 2940 3000 3318 Size: 393 390 158 477 147 136 60 318 Cuts at: 3318 4187 4251 4356 4416 5085 5109 6498 6941 Size: 869 64 105 60 669 24 1389 443 Cuts at: 6941 8008 8450 8783 8962 10010 10607 11394 11662 Size: 1067 442 333 179 1048 597 787 268 Cuts at: 11662 1239 Size: 1524 Fragments arranged by size: 1524 1389 1067 1048 869 787 669 597 477 443 442 393 390 333 318 268 179 158 147 136 105 64 60 60 24 EcoNI CCTnn'n_nnAGG Cuts at: 2346 2892 5955 2346 Size: 546 3063 8338 Fragments arranged by size: 8338 3063 546 EcoO109I rG'GnC_Cy Cuts at: 2441 2717 3665 4019 7540 7763 7764 7928 8609 Size: 276 948 354 3521 223 1 164 681 Cuts at: 8609 8814 2441 Size: 205 5574 Fragments arranged by size: 5574 3521 948 681 354 276 223 205 164 1 EcoRI G'AATT_C Cuts at: 3702 4492 4891 9112 9211 9256 9634 3702 Size: 790 399 4221 99 45 378 6015 Fragments arranged by size: 6015 4221 790 399 378 99 45 FspI TGC'GCA Cuts at: 10453 10453 Size: 11947 HaeII r_GCGC'y Cuts at: 1678 3064 7216 7825 9174 11094 1678 Size: 1386 4152 609 1349 1920 2531 Fragments arranged by size: 4152 2531 1920 1386 1349 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2455 2487 2653 Size: 32 135 32 518 32 333 32 166 Cuts at: 2653 2685 3374 3406 3523 3555 4099 4131 5201 Size: 32 689 32 117 32 544 32 1070 Cuts at: 5201 5233 6865 6897 7912 7944 8674 8706 8986 Size: 32 1632 32 1015 32 730 32 280 Cuts at: 8986 9018 10588 10620 10662 10694 1373 Size: 32 1570 32 42 32 2626 Fragments arranged by size: 2626 1632 1570 1070 1015 730 689 544 518 333 280 166 135 117 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2454 2915 3344 5234 6112 8072 Size: 988 281 516 461 429 1890 878 1960 Cuts at: 8072 9291 9537 669 Size: 1219 246 3079 Fragments arranged by size: 3079 1960 1890 1219 988 878 516 461 429 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 11947 HpaI GTT'AAC Cuts at: 9537 9537 Size: 11947 KpnI G_GTAC'C Cuts at: 9272 9272 Size: 11947 MfeI C'AATT_G Cuts at: 2589 7258 9546 2589 Size: 4669 2288 4990 Fragments arranged by size: 4990 4669 2288 MluI A'CGCG_T Cuts at: 11823 11823 Size: 11947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3289 4305 5487 7229 7592 7751 7885 11061 Size: 1984 1016 1182 1742 363 159 134 3176 Cuts at: 11061 11245 1305 Size: 184 2007 Fragments arranged by size: 3176 2007 1984 1742 1182 1016 363 184 159 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 4152 7502 7584 8621 Size: 54 1425 343 486 1834 3350 82 1037 Cuts at: 8621 10 Size: 3336 Fragments arranged by size: 3350 3336 1834 1425 1037 486 343 82 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 2495 2507 4886 5604 6620 6962 Size: 648 1248 81 12 2379 718 1016 342 Cuts at: 6962 6968 7901 9905 10264 10423 518 Size: 6 933 2004 359 159 2042 Fragments arranged by size: 2379 2042 2004 1248 1016 933 718 648 359 342 159 81 12 6 MspA1I CmG'CkG Cuts at: 1606 2245 2993 4362 4795 4835 7456 7513 7811 Size: 639 748 1369 433 40 2621 57 298 Cuts at: 7811 8242 8266 8680 9363 10040 11188 11433 1606 Size: 431 24 414 683 677 1148 245 2120 Fragments arranged by size: 2621 2120 1369 1148 748 683 677 639 433 431 414 298 245 57 40 24 NcoI C'CATG_G Cuts at: 513 1065 2004 2490 4210 5292 6765 7887 7896 Size: 552 939 486 1720 1082 1473 1122 9 Cuts at: 7896 8475 9078 9160 513 Size: 579 603 82 3300 Fragments arranged by size: 3300 1720 1473 1122 1082 939 603 579 552 486 82 9 NdeI CA'TA_TG Cuts at: 387 3835 8853 387 Size: 3448 5018 3481 Fragments arranged by size: 5018 3481 3448 NheI G'CTAG_C Cuts at: 1052 9134 1052 Size: 8082 3865 NotI GC'GGCC_GC Cuts at: 9394 9394 Size: 11947 NsiI A_TGCA'T Cuts at: 5769 6135 5769 Size: 366 11581 NspI r_CATG'y Cuts at: 1132 1229 3047 3514 3862 3976 6133 6137 6318 Size: 97 1818 467 348 114 2157 4 181 Cuts at: 6318 6967 7510 7654 8323 8698 9305 10850 1132 Size: 649 543 144 669 375 607 1545 2229 Fragments arranged by size: 2229 2157 1818 1545 669 649 607 543 467 375 348 181 144 114 97 4 PasI CC'CwG_GG Cuts at: 1348 3661 7696 8306 8326 1348 Size: 2313 4035 610 20 4969 Fragments arranged by size: 4969 4035 2313 610 20 PciI A'CATG_T Cuts at: 3858 10846 3858 Size: 6988 4959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2971 4133 7117 7228 8245 8749 9050 1108 Size: 1863 1162 2984 111 1017 504 301 4005 Fragments arranged by size: 4005 2984 1863 1162 1017 504 301 111 PfoI T'CCnGG_A Cuts at: 2341 2527 2785 5125 9049 2341 Size: 186 258 2340 3924 5239 Fragments arranged by size: 5239 3924 2340 258 186 PmeI GTTT'AAAC Cuts at: 9251 9251 Size: 11947 PmlI CAC'GTG Cuts at: 7837 7837 Size: 11947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10016 10048 11529 11561 10016 Size: 32 1481 32 10402 Fragments arranged by size: 10402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2441 3665 7928 8814 2441 Size: 1224 4263 886 5574 Fragments arranged by size: 5574 4263 1224 886 PshAI GACnn'nnGTC Cuts at: 1613 6239 1613 Size: 4626 7321 PsiI TTA'TAA Cuts at: 9517 9517 Size: 11947 PspOMI G'GGCC_C Cuts at: 7763 7763 Size: 11947 PstI C_TGCA'G Cuts at: 830 2545 3991 6241 6396 7171 7461 7777 8202 Size: 1715 1446 2250 155 775 290 316 425 Cuts at: 8202 9299 830 Size: 1097 3478 Fragments arranged by size: 3478 2250 1715 1446 1097 775 425 316 290 155 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11947 PvuII CAG'CTG Cuts at: 2245 2993 4835 7456 8242 2245 Size: 748 1842 2621 786 5950 Fragments arranged by size: 5950 2621 1842 786 748 SacI G_AGCT'C Cuts at: 4237 4237 Size: 11947 SalI G'TCGA_C Cuts at: 1936 9289 1936 Size: 7353 4594 SanDI GG'GwC_CC Cuts at: 8814 8814 Size: 11947 SapI GCTCTTCn'nnn_ Cuts at: 4488 5279 6934 4488 Size: 791 1655 9501 Fragments arranged by size: 9501 1655 791 SbfI CC_TGCA'GG Cuts at: 9299 9299 Size: 11947 ScaI AGT'ACT Cuts at: 5098 10195 11907 5098 Size: 5097 1712 5138 Fragments arranged by size: 5138 5097 1712 SfcI C'TryA_G Cuts at: 826 1045 2154 2541 3959 3987 6237 6392 7167 Size: 219 1109 387 1418 28 2250 155 775 Cuts at: 7167 7457 7469 7773 8021 8198 8342 8774 9295 Size: 290 12 304 248 177 144 432 521 Cuts at: 9295 10430 11111 11302 826 Size: 1135 681 191 1471 Fragments arranged by size: 2250 1471 1418 1135 1109 775 681 521 432 387 304 290 248 219 191 177 155 144 28 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2324 2324 Size: 11947 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11947 SmaI CCC'GGG Cuts at: 1872 6718 9274 1872 Size: 4846 2556 4545 Fragments arranged by size: 4846 4545 2556 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 4380 6229 7674 8551 10051 Size: 197 926 786 1651 1849 1445 877 1500 Cuts at: 10051 10952 11214 820 Size: 901 262 1553 Fragments arranged by size: 1849 1651 1553 1500 1445 926 901 877 786 262 197 SnaBI TAC'GTA Cuts at: 493 9738 493 Size: 9245 2702 SpeI A'CTAG_T Cuts at: 152 2423 4874 152 Size: 2271 2451 7225 Fragments arranged by size: 7225 2451 2271 SphI G_CATG'C Cuts at: 1132 3047 3514 6133 6967 7510 7654 8323 9305 Size: 1915 467 2619 834 543 144 669 982 Cuts at: 9305 1132 Size: 3774 Fragments arranged by size: 3774 2619 1915 982 834 669 543 467 144 SspI AAT'ATT Cuts at: 5 52 9871 5 Size: 47 9819 2081 Fragments arranged by size: 9819 2081 47 StuI AGG'CCT Cuts at: 1517 2946 7131 8747 8780 1517 Size: 1429 4185 1616 33 4684 Fragments arranged by size: 4684 4185 1616 1429 33 StyI C'CwwG_G Cuts at: 513 1065 2004 2490 2812 2965 3505 4210 4649 Size: 552 939 486 322 153 540 705 439 Cuts at: 4649 5292 5482 5629 6627 6765 7132 7308 7887 Size: 643 190 147 998 138 367 176 579 Cuts at: 7887 7896 7993 8036 8475 8647 8748 8990 9078 Size: 9 97 43 439 172 101 242 88 Cuts at: 9078 9160 9386 513 Size: 82 226 3074 Fragments arranged by size: 3074 998 939 705 643 579 552 540 486 439 439 367 322 242 226 190 176 172 153 147 138 101 97 88 82 43 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6181 7190 7975 8488 9974 9991 10144 Size: 1255 4601 1009 785 513 1486 17 153 Cuts at: 10144 10329 10774 325 Size: 185 445 1498 Fragments arranged by size: 4601 1498 1486 1255 1009 785 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2839 3221 5096 Size: 274 80 33 51 1443 862 382 1875 Cuts at: 5096 5744 7052 9230 10193 11905 96 Size: 648 1308 2178 963 1712 138 Fragments arranged by size: 2178 1875 1712 1443 1308 963 862 648 382 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5707 5739 8286 8318 9008 9040 11907 11939 5707 Size: 32 2547 32 690 32 2867 32 5715 Fragments arranged by size: 5715 2867 2547 690 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11947 XbaI T'CTAG_A Cuts at: 5908 9283 5908 Size: 3375 8572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 4951 6964 7894 7939 8053 2413 Size: 2538 2013 930 45 114 6307 Fragments arranged by size: 6307 2538 2013 930 114 45 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 11947 XmaI C'CCGG_G Cuts at: 1870 6716 9272 1870 Size: 4846 2556 4545 Fragments arranged by size: 4846 4545 2556 XmnI GAAnn'nnTTC Cuts at: 4051 5220 5615 6060 10076 4051 Size: 1169 395 445 4016 5922 Fragments arranged by size: 5922 4016 1169 445 395 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9755 276 Size: 53 83 186 936 8221 2468 Fragments arranged by size: 8221 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BsiWI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI