Restriction map for
pFN21AB4815
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaBI
BsaWI BseAI BspEI BsrFI BssHII
BssSI BstBI BstUI Bsu36I Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1246 GGTACC G^GTACC
AseI 1948 ATTAAT AT^TAAT
AsnI 1948 ATTAAT AT^TAAT
Asp718I 1246 GGTACC G^GTACC
AspI 2689 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 504 GGATCC G^GATCC
BlpI 1831 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1831 GCTGAGC GC^T[ACGT]AGC
BsaAI 2108 TACGTG [CT]AC^GT[GA]
BsiEI 4136 CGATCG CG[GA][CT]^CG
BsiWI 2190 CGTACG C^GTACG
BsmBI 4360 GAGACG CGTCTC(1/5)
BspDI 3172 ATCGAT AT^CGAT
BspLU11I 786 ACATGT A^CATGT
BsrBI 4116 CCGCTC CCGCTC(-3/-3)
Bst1107I 493 GTATAC GTA^TAC
BstAPI 4035 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 493 GTATAC GTA^TAC
CelII 1831 GCTGAGC GC^T[ACGT]AGC
ClaI 3172 ATCGAT AT^CGAT
DraIII 2286 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 4254 CCTTGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1085 GAATTC G^AATTC
FauI 2494 CCCGC CCCGC(4/6)
HaeII 3551 AGCGCC [GA]GCGC^[CT]
KpnI 1246 GGTACC GGTAC^C
MfeI 4376 CAATTG C^AATTG
MunI 4376 CAATTG C^AATTG
PaeR7I 3155 CTCGAG C^TCGAG
PflFI 2689 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2764 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4221 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1948 ATTAAT AT^TAAT
PstI 3898 CTGCAG CTGCA^G
PvuI 4136 CGATCG CGAT^CG
SapI 2170 GAAGAGC GCTCTTC(1/4)
SpeI 489 ACTAGT A^CTAGT
SspI 3991 AATATT AAT^ATT
StuI 2417 AGGCCT AGG^CCT
Tth111I 2689 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2764 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3155 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 786 ACATGT A^C[GA][CT]GT
2109 ACGTGT
Asp700I 457 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
667 GAATTTGTTC
AvaI 2831 CCCGAG C^[CT]CG[GA]G
3155 CTCGAG
BalI 1410 TGGCCA TGG^CCA
1691 TGGCCA
BbvCI 2254 GCTGAGG CCTCAGC(-5/-2)
3610 GCTGAGG
BcnI 1643 CCGGG CC^[GC]GG
2466 CCGGG
BsmI 3726 GAATGC GAATGC(1/-1)
2029 GCATTC
BsoBI 2831 CCCGAG C^[CT]CG[GA]G
3155 CTCGAG
Bsp1407I 1212 TGTACA T^GTACA
1997 TGTACA
BspHI 1395 TCATGA T^CATGA
1817 TCATGA
BsrGI 1212 TGTACA T^GTACA
1997 TGTACA
BstEII 3031 GGTTACC G^GT[ACGT]ACC
3472 GGTAACC
BstPI 3031 GGTTACC G^GT[ACGT]ACC
3472 GGTAACC
EaeI 1410 TGGCCA [CT]^GGCC[GA]
1691 TGGCCA
EcoO65I 3031 GGTTACC G^GT[ACGT]ACC
3472 GGTAACC
EcoT22I 2089 ATGCAT ATGCA^T
2460 ATGCAT
MluNI 1410 TGGCCA TGG^CCA
1691 TGGCCA
MscI 1410 TGGCCA TGG^CCA
1691 TGGCCA
NciI 1643 CCGGG CC^[GC]GG
2466 CCGGG
NdeI 764 CATATG CA^TATG
3201 CATATG
NsiI 2089 ATGCAT ATGCA^T
2460 ATGCAT
NspI 786 ACATGT [GA]CATG^[CT]
2163 GCATGT
PpuMI 1874 AGGACCT [GA]G^G[AT]CC[CT]
3677 AGGACCT
PvuII 928 CAGCTG CAG^CTG
2479 CAGCTG
RcaI 1395 TCATGA T^CATGA
1817 TCATGA
ScaI 2198 AGTACT AGT^ACT
3495 AGTACT
SfcI 3072 CTACAG C^T[GA][CT]AG
3898 CTGCAG
SspBI 1212 TGTACA T^GTACA
1997 TGTACA
XmnI 457 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
667 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII