Restriction map for
pFN21AB4815
(Linear) (Six-base) MAPSORT of: KIBB4815.seq Check: 4068 from: 1 to: 4422
KIBB4815.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1092 1760 2236 4422
Size: 1092 668 476 2186
Fragments arranged by size:
2186 1092 668 476
AccI GT'mk_AC
Cuts at: 0 494 2106 3965 4422
Size: 494 1612 1859 457
Fragments arranged by size:
1859 1612 494 457
Acc65I G'GTAC_C
Cuts at: 0 1246 4422
Size: 1246 3176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1489 1698 2120 2339 2648 2741 2804
Size: 952 537 209 422 219 309 93 63
Cuts at: 2804 3157 3830 4195 4422
Size: 353 673 365 227
Fragments arranged by size:
952 673 537 422 365 353 309 227
219 209 93 63
AflIII A'CryG_T
Cuts at: 0 786 2109 4422
Size: 786 1323 2313
Fragments arranged by size:
2313 1323 786
AleI CACnn'nnGTG
Cuts at: 0 3763 4422
Size: 3763 659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3743 4422
Size: 3743 679
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 4422
Size: 286 32 4104
Fragments arranged by size:
4104 286 32
AlwNI CAG_nnn'CTG
Cuts at: 0 76 175 2275 2671 4041 4278 4422
Size: 76 99 2100 396 1370 237 144
Fragments arranged by size:
2100 1370 396 237 144 99 76
ApoI r'AATT_y
Cuts at: 0 337 433 667 865 1085 2604 3865 4422
Size: 337 96 234 198 220 1519 1261 557
Fragments arranged by size:
1519 1261 557 337 234 220 198 96
AseI AT'TA_AT
Cuts at: 0 1949 4422
Size: 1949 2473
AvaI C'yCGr_G
Cuts at: 0 2831 3155 4422
Size: 2831 324 1267
Fragments arranged by size:
2831 1267 324
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1955 1988 4422
Size: 1955 33 2434
Fragments arranged by size:
2434 1955 33
BamHI G'GATC_C
Cuts at: 0 504 4422
Size: 504 3918
BanI G'GyrC_C
Cuts at: 0 1246 1453 2658 3570 4422
Size: 1246 207 1205 912 852
Fragments arranged by size:
1246 1205 912 852 207
BanII G_rGCy'C
Cuts at: 0 593 604 1388 1838 2387 3247 4422
Size: 593 11 784 450 549 860 1175
Fragments arranged by size:
1175 860 784 593 549 450 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 915 1559 1925 3145 3403 3836 4422
Size: 272 643 644 366 1220 258 433 586
Fragments arranged by size:
1220 644 643 586 433 366 272 258
BbvCI CC'TCA_GC
Cuts at: 0 2255 3611 4422
Size: 2255 1356 811
Fragments arranged by size:
2255 1356 811
BfrBI ATG'CAT
Cuts at: 0 2091 2462 4422
Size: 2091 371 1960
Fragments arranged by size:
2091 1960 371
BglII A'GATC_T
Cuts at: 0 4201 4320 4407 4422
Size: 4201 119 87 15
Fragments arranged by size:
4201 119 87 15
BlpI GC'TnA_GC
Cuts at: 0 1832 4422
Size: 1832 2590
BmgBI CAC'GTC
Cuts at: 0 1650 4422
Size: 1650 2772
BmrI ACTGGGnnnn_n'
Cuts at: 0 1532 2506 3824 4306 4422
Size: 1532 974 1318 482 116
Fragments arranged by size:
1532 1318 974 482 116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1952 2881 2975 3349 3403 3955 4422
Size: 250 1702 929 94 374 54 552 467
Fragments arranged by size:
1702 929 552 467 374 250 94 54
Bpu10I CC'TnA_GC
Cuts at: 0 2255 3611 4422
Size: 2255 1356 811
Fragments arranged by size:
2255 1356 811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1402 2413 2621 3484 4422
Size: 544 858 1011 208 863 938
Fragments arranged by size:
1011 938 863 858 544 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 2179 3912 4161 4422
Size: 118 2061 1733 249 261
Fragments arranged by size:
2061 1733 261 249 118
BsaAI yAC'GTr
Cuts at: 0 2110 4422
Size: 2110 2312
BsaHI Gr'CG_yC
Cuts at: 0 65 1127 1843 4422
Size: 65 1062 716 2579
Fragments arranged by size:
2579 1062 716 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 286 316 1165 1195 1499 1529 1715 1745
Size: 286 30 849 30 304 30 186 30
Cuts at: 1745 1752 1782 1950 1980 2255 2285 4422
Size: 7 30 168 30 275 30 2137
Fragments arranged by size:
2137 849 304 286 275 186 168 30
30 30 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 307 1760 3293 3628 3735 4422
Size: 31 276 1453 1533 335 107 687
Fragments arranged by size:
1533 1453 687 335 276 107 31
BseYI C'CCAG_C
Cuts at: 0 1461 1734 3656 4422
Size: 1461 273 1922 766
Fragments arranged by size:
1922 1461 766 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2497 3809 4054 4422
Size: 2497 1312 245 368
Fragments arranged by size:
2497 1312 368 245
BsiEI CG_ry'CG
Cuts at: 0 4139 4422
Size: 4139 283
BsiHKAI G_wGCw'C
Cuts at: 0 650 1838 2387 3247 3638 4422
Size: 650 1188 549 860 391 784
Fragments arranged by size:
1188 860 784 650 549 391
BsiWI C'GTAC_G
Cuts at: 0 2190 4422
Size: 2190 2232
BsmI GAATG_Cn'
Cuts at: 0 2029 3732 4422
Size: 2029 1703 690
Fragments arranged by size:
2029 1703 690
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4354 4422
Size: 4354 68
Bsp1286I G_dGCh'C
Cuts at: 0 593 604 650 1388 1838 2387 3247 3638
Size: 593 11 46 738 450 549 860 391
Cuts at: 3638 4422
Size: 784
Fragments arranged by size:
860 784 738 593 549 450 391 46
11
BspHI T'CATG_A
Cuts at: 0 1395 1817 4422
Size: 1395 422 2605
Fragments arranged by size:
2605 1395 422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1092 1760 1868 2236 3460 3894 4422
Size: 1092 668 108 368 1224 434 528
Fragments arranged by size:
1224 1092 668 528 434 368 108
BsrBI CCG'CTC
Cuts at: 0 4118 4422
Size: 4118 304
BsrDI GCAATG_nn'
Cuts at: 0 1598 3372 3910 4422
Size: 1598 1774 538 512
Fragments arranged by size:
1774 1598 538 512
BsrGI T'GTAC_A
Cuts at: 0 1212 1997 4422
Size: 1212 785 2425
Fragments arranged by size:
2425 1212 785
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4041 4422
Size: 4041 381
BstEII G'GTnAC_C
Cuts at: 0 3031 3472 4422
Size: 3031 441 950
Fragments arranged by size:
3031 950 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1242 1701 4422
Size: 360 882 459 2721
Fragments arranged by size:
2721 882 459 360
BstYI r'GATC_y
Cuts at: 0 504 974 2216 4201 4320 4407 4422
Size: 504 470 1242 1985 119 87 15
Fragments arranged by size:
1985 1242 504 470 119 87 15
BstZ17I GTA'TAC
Cuts at: 0 495 4422
Size: 495 3927
BtgI C'CryG_G
Cuts at: 0 449 659 834 888 1193 2435 3417 4422
Size: 449 210 175 54 305 1242 982 1005
Fragments arranged by size:
1242 1005 982 449 305 210 175 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 507 3303 4422
Size: 507 2796 1119
Fragments arranged by size:
2796 1119 507
BtsI GCAGTG_nn'
Cuts at: 0 1337 2345 2535 3253 3442 4422
Size: 1337 1008 190 718 189 980
Fragments arranged by size:
1337 1008 980 718 190 189
ClaI AT'CG_AT
Cuts at: 0 3173 4422
Size: 3173 1249
DraIII CAC_nnn'GTG
Cuts at: 0 2291 4422
Size: 2291 2131
DrdI GACnn_nn'nnGTC
Cuts at: 0 70 382 2102 3538 3840 4422
Size: 70 312 1720 1436 302 582
Fragments arranged by size:
1720 1436 582 312 302 70
EaeI y'GGCC_r
Cuts at: 0 1410 1691 4422
Size: 1410 281 2731
Fragments arranged by size:
2731 1410 281
EarI CTCTTCn'nnn_
Cuts at: 0 223 725 928 1319 2136 2165 2346 4004
Size: 223 502 203 391 817 29 181 1658
Cuts at: 4004 4422
Size: 418
Fragments arranged by size:
1658 817 502 418 391 223 203 181
29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 3294 4422
Size: 250 3044 1128
Fragments arranged by size:
3044 1128 250
EcoICRI GAG'CTC
Cuts at: 0 1836 2385 3245 4422
Size: 1836 549 860 1177
Fragments arranged by size:
1836 1177 860 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 952 1489 1698 1952 2120 2339 2648
Size: 250 702 537 209 254 168 219 309
Cuts at: 2648 2741 2804 2881 2975 3157 3349 3403 3830
Size: 93 63 77 94 182 192 54 427
Cuts at: 3830 3955 4195 4422
Size: 125 240 227
Fragments arranged by size:
702 537 427 309 254 250 240 227
219 209 192 182 168 125 94 93
77 63 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4258 4422
Size: 4258 164
EcoO109I rG'GnC_Cy
Cuts at: 0 1875 2303 3678 4422
Size: 1875 428 1375 744
Fragments arranged by size:
1875 1375 744 428
EcoRI G'AATT_C
Cuts at: 0 1085 4422
Size: 1085 3337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1497 4422
Size: 1497 2925
HaeII r_GCGC'y
Cuts at: 0 3555 4422
Size: 3555 867
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1375 1407 1499 1531 2255 2287 2321 2353
Size: 1375 32 92 32 724 32 34 32
Cuts at: 2353 2825 2857 2986 3018 3149 3181 3610 3642
Size: 472 32 129 32 131 32 429 32
Cuts at: 3642 3670 3702 4029 4061 4249 4281 4422
Size: 28 32 327 32 188 32 141
Fragments arranged by size:
1375 724 472 429 327 188 141 131
129 92 34 32 32 32 32 32
32 32 32 32 32 32 28
KpnI G_GTAC'C
Cuts at: 0 1250 4422
Size: 1250 3172
MfeI C'AATT_G
Cuts at: 0 4376 4422
Size: 4376 46
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 4151 4422
Size: 851 3300 271
Fragments arranged by size:
3300 851 271
MscI TGG'CCA
Cuts at: 0 1412 1693 4422
Size: 1412 281 2729
Fragments arranged by size:
2729 1412 281
MslI CAynn'nnrTG
Cuts at: 0 358 3062 3763 3943 4422
Size: 358 2704 701 180 479
Fragments arranged by size:
2704 701 479 358 180
MspA1I CmG'CkG
Cuts at: 0 930 1765 2481 4422
Size: 930 835 716 1941
Fragments arranged by size:
1941 930 835 716
NcoI C'CATG_G
Cuts at: 0 449 888 3417 4422
Size: 449 439 2529 1005
Fragments arranged by size:
2529 1005 449 439
NdeI CA'TA_TG
Cuts at: 0 765 3202 4422
Size: 765 2437 1220
Fragments arranged by size:
2437 1220 765
NsiI A_TGCA'T
Cuts at: 0 2093 2464 4422
Size: 2093 371 1958
Fragments arranged by size:
2093 1958 371
NspI r_CATG'y
Cuts at: 0 790 2167 4422
Size: 790 1377 2255
Fragments arranged by size:
2255 1377 790
PasI CC'CwG_GG
Cuts at: 0 1891 3732 4422
Size: 1891 1841 690
Fragments arranged by size:
1891 1841 690
PciI A'CATG_T
Cuts at: 0 786 4422
Size: 786 3636
PflMI CCAn_nnn'nTGG
Cuts at: 0 2770 4422
Size: 2770 1652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 1512 1544 4422
Size: 286 32 1194 32 2878
Fragments arranged by size:
2878 1194 286 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1875 3678 4422
Size: 1875 1803 744
Fragments arranged by size:
1875 1803 744
PshAI GACnn'nnGTC
Cuts at: 0 4225 4422
Size: 4225 197
PspXI vC'TCGA_Gb
Cuts at: 0 3155 4422
Size: 3155 1267
PstI C_TGCA'G
Cuts at: 0 3902 4422
Size: 3902 520
PvuI CG_AT'CG
Cuts at: 0 4139 4422
Size: 4139 283
PvuII CAG'CTG
Cuts at: 0 930 2481 4422
Size: 930 1551 1941
Fragments arranged by size:
1941 1551 930
SacI G_AGCT'C
Cuts at: 0 1838 2387 3247 4422
Size: 1838 549 860 1175
Fragments arranged by size:
1838 1175 860 549
SapI GCTCTTCn'nnn_
Cuts at: 0 2165 4422
Size: 2165 2257
ScaI AGT'ACT
Cuts at: 0 2200 3497 4422
Size: 2200 1297 925
Fragments arranged by size:
2200 1297 925
SfcI C'TryA_G
Cuts at: 0 3072 3898 4422
Size: 3072 826 524
Fragments arranged by size:
3072 826 524
SmlI C'TyrA_G
Cuts at: 0 559 1381 2428 2636 3155 3499 4422
Size: 559 822 1047 208 519 344 923
Fragments arranged by size:
1047 923 822 559 519 344 208
SpeI A'CTAG_T
Cuts at: 0 489 4422
Size: 489 3933
SspI AAT'ATT
Cuts at: 0 3993 4422
Size: 3993 429
StuI AGG'CCT
Cuts at: 0 2419 4422
Size: 2419 2003
StyI C'CwwG_G
Cuts at: 0 188 449 888 917 3005 3417 3566 4422
Size: 188 261 439 29 2088 412 149 856
Fragments arranged by size:
2088 856 439 412 261 188 149 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1296 3766 4422
Size: 1296 2470 656
Fragments arranged by size:
2470 1296 656
TatI w'GTAC_w
Cuts at: 0 690 1212 1978 1997 2198 2955 3495 4385
Size: 690 522 766 19 201 757 540 890
Cuts at: 4385 4422
Size: 37
Fragments arranged by size:
890 766 757 690 540 522 201 37
19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 980 1012 1526 1558 1715 1747 1752 1784
Size: 980 32 514 32 157 32 5 32
Cuts at: 1784 1950 1982 4422
Size: 166 32 2440
Fragments arranged by size:
2440 980 514 166 157 32 32 32
32 32 5
Tth111I GACn'n_nGTC
Cuts at: 0 2692 4422
Size: 2692 1730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 550 1239 2905 3324 3444 3573 4422
Size: 550 689 1666 419 120 129 849
Fragments arranged by size:
1666 849 689 550 419 129 120
XhoI C'TCGA_G
Cuts at: 0 3155 4422
Size: 3155 1267
XmnI GAAnn'nnTTC
Cuts at: 0 461 671 4422
Size: 461 210 3751
Fragments arranged by size:
3751 461 210
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BfrBI BglII BlpI BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrGI BstAPI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I KpnI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PflMI PpiI
PpuMI PshAI PspXI PstI PvuI PvuII SacI SapI
ScaI SfcI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI ApaI ApaLI
AscI AsiSI AvrII BbeI BcgI BciVI BclI BglI
Bme1580I BmtI BplI BsaBI BsaWI BspEI BsrFI BssHII
BssSI BstBI Bsu36I CspCI DraI EagI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PfoI PmeI
PmlI PsiI PspOMI PsrI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI XbaI XmaI ZraI