(Circular) (Six-base) MAPSORT of: pFN21AB4802.seq Check: 7054 from: 1 to: 8221 pFN21AB4802 8221 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4495 5522 4495 Size: 1027 7194 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6031 278 Size: 53 83 186 936 4495 2468 Fragments arranged by size: 4495 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3805 5564 1937 Size: 1868 1759 4594 Fragments arranged by size: 4594 1868 1759 Acc65I G'GTAC_C Cuts at: 5542 5542 Size: 8221 AclI AA'CG_TT Cuts at: 1563 6348 6721 1563 Size: 4785 373 3063 Fragments arranged by size: 4785 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4122 6284 7668 7936 1632 Size: 2490 2162 1384 268 1917 Fragments arranged by size: 2490 2162 1917 1384 268 AfeI AGC'GCT Cuts at: 2408 2879 3230 3541 4610 2408 Size: 471 351 311 1069 6019 Fragments arranged by size: 6019 1069 471 351 311 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8024 AflIII A'CryG_T Cuts at: 1203 2975 7120 8097 1203 Size: 1772 4145 977 1327 Fragments arranged by size: 4145 1772 1327 977 AgeI A'CCGG_T Cuts at: 5274 7052 5274 Size: 1778 6443 AhdI GACnn_n'nnGTC Cuts at: 6950 6950 Size: 8221 AleI CACnn'nnGTG Cuts at: 1166 2819 3294 1166 Size: 1653 475 6093 Fragments arranged by size: 6093 1653 475 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 4887 1184 Size: 1690 2013 4518 Fragments arranged by size: 4518 2013 1690 AlwNI CAG_nnn'CTG Cuts at: 2449 2530 3793 3842 3997 4784 5068 5964 7536 Size: 81 1263 49 155 787 284 896 1572 Cuts at: 7536 2449 Size: 3134 Fragments arranged by size: 3134 1572 1263 896 787 284 155 81 49 ApaI G_GGCC'C Cuts at: 2382 3635 3690 2382 Size: 1253 55 6913 Fragments arranged by size: 6913 1253 55 ApaLI G'TGCA_C Cuts at: 2883 6278 7434 2883 Size: 3395 1156 3670 Fragments arranged by size: 3670 3395 1156 ApoI r'AATT_y Cuts at: 1493 2625 5530 5756 5908 8017 1493 Size: 1132 2905 226 152 2109 1697 Fragments arranged by size: 2905 2109 1697 1132 226 152 AseI AT'TA_AT Cuts at: 160 1032 6775 160 Size: 872 5743 1606 Fragments arranged by size: 5743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8221 AvaI C'yCGr_G Cuts at: 1870 1943 2478 3106 3634 5546 1870 Size: 73 535 628 528 1912 4545 Fragments arranged by size: 4545 1912 628 535 528 73 BamHI G'GATC_C Cuts at: 3873 5316 5551 5918 3873 Size: 1443 235 367 6176 Fragments arranged by size: 6176 1443 367 235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2520 3102 4286 5012 5201 Size: 325 217 627 733 582 1184 726 189 Cuts at: 5201 5309 5542 6997 618 Size: 108 233 1455 1842 Fragments arranged by size: 1842 1455 1184 733 726 627 582 325 233 217 189 108 BanII G_rGCy'C Cuts at: 1391 2382 3175 3635 3690 4924 5142 5540 1391 Size: 991 793 460 55 1234 218 398 4072 Fragments arranged by size: 4072 1234 991 793 460 398 218 55 BbeI G_GCGC'C Cuts at: 3106 3106 Size: 8221 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4320 5336 928 Size: 971 2421 1016 3813 Fragments arranged by size: 3813 2421 1016 971 BbvCI CC'TCA_GC Cuts at: 3470 4896 4902 5052 3470 Size: 1426 6 150 6639 Fragments arranged by size: 6639 1426 150 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2498 2532 4562 4596 4700 4734 6412 Size: 34 561 34 2030 34 104 34 1678 Cuts at: 6412 6446 1903 Size: 34 3678 Fragments arranged by size: 3678 2030 1678 561 104 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6113 7323 6113 Size: 1210 7011 BclI T'GATC_A Cuts at: 1548 1548 Size: 8221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2539 3675 3711 Size: 107 122 71 631 605 867 1136 36 Cuts at: 3711 4484 4754 6832 136 Size: 773 270 2078 1525 Fragments arranged by size: 2078 1525 1136 867 773 631 605 270 122 107 71 36 BglII A'GATC_T Cuts at: 8216 8216 Size: 8221 BlpI GC'TnA_GC Cuts at: 4157 4191 4512 5434 5638 4157 Size: 34 321 922 204 6740 Fragments arranged by size: 6740 922 321 204 34 Bme1580I G_kGCm'C Cuts at: 1790 2382 2688 2887 2967 3506 3635 3690 4606 Size: 592 306 199 80 539 129 55 916 Cuts at: 4606 4882 5227 5314 6282 7438 1790 Size: 276 345 87 968 1156 2573 Fragments arranged by size: 2573 1156 968 916 592 539 345 306 276 199 129 87 80 55 BmgBI CAC'GTC Cuts at: 1318 3583 1318 Size: 2265 5956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2170 3179 5952 Size: 416 53 83 399 24 753 1009 2773 Cuts at: 5952 6910 442 Size: 958 1753 Fragments arranged by size: 2773 1753 1009 958 753 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8221 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2428 3932 2428 Size: 1504 6717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 2929 3869 3974 4037 4070 4340 4360 Size: 961 729 940 105 63 33 270 20 Cuts at: 4360 4526 5474 6881 1239 Size: 166 948 1407 2579 Fragments arranged by size: 2579 1407 961 948 940 729 270 166 105 63 33 20 Bpu10I CC'TnA_GC Cuts at: 3470 3951 4229 4896 4902 4995 5052 3470 Size: 481 278 667 6 93 57 6639 Fragments arranged by size: 6639 667 481 278 93 57 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2674 6346 7211 7509 2674 Size: 3672 865 298 3386 Fragments arranged by size: 3672 3386 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2002 2156 2905 3119 4038 4082 4204 Size: 617 503 154 749 214 919 44 122 Cuts at: 4204 4610 5351 6884 882 Size: 406 741 1533 2219 Fragments arranged by size: 2219 1533 919 749 741 617 503 406 214 154 122 44 BsaAI yAC'GTr Cuts at: 493 1204 6012 493 Size: 711 4808 2702 Fragments arranged by size: 4808 2702 711 BsaBI GATnn'nnATC Cuts at: 8215 8215 Size: 8221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2931 3103 6028 6410 Size: 53 83 186 936 1398 172 2925 382 Cuts at: 6410 7045 275 Size: 635 1451 Fragments arranged by size: 2925 1451 1398 936 635 382 186 172 83 53 BsaWI w'CCGG_w Cuts at: 3093 3151 3567 4149 5274 6654 7052 7326 7473 Size: 58 416 582 1125 1380 398 274 147 Cuts at: 7473 7884 3093 Size: 411 3430 Fragments arranged by size: 3430 1380 1125 582 416 411 398 274 147 58 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 4013 4016 4043 Size: 30 165 30 1047 30 1533 3 27 Cuts at: 4043 4046 1178 Size: 3 5353 Fragments arranged by size: 5353 1533 1047 165 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2401 3356 4034 4037 4290 4293 4296 5150 Size: 1215 955 678 3 253 3 3 854 Cuts at: 5150 5357 1186 Size: 207 4050 Fragments arranged by size: 4050 1215 955 854 678 253 207 3 3 3 BseYI C'CCAG_C Cuts at: 2708 2730 4536 4605 5110 5184 5296 7424 7920 Size: 22 1806 69 505 74 112 2128 496 Cuts at: 7920 2708 Size: 3009 Fragments arranged by size: 3009 2128 1806 505 496 112 74 69 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2155 2446 2843 3068 3239 3556 1163 Size: 573 419 291 397 225 171 317 5828 Fragments arranged by size: 5828 573 419 397 317 291 225 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5671 6432 7460 1540 Size: 275 186 3670 761 1028 2301 Fragments arranged by size: 3670 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2887 3403 4163 5142 5502 6282 6367 7438 2887 Size: 516 760 979 360 780 85 1071 3670 Fragments arranged by size: 3670 1071 979 780 760 516 360 85 BsmI GAATG_Cn' Cuts at: 2945 5732 5825 2945 Size: 2787 93 5341 Fragments arranged by size: 5341 2787 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3573 4019 3573 Size: 446 7775 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2688 2887 2967 3175 3403 3506 Size: 399 592 306 199 80 208 228 103 Cuts at: 3506 3635 3690 4163 4606 4882 4924 5142 5227 Size: 129 55 473 443 276 42 218 85 Cuts at: 5227 5314 5502 5540 6282 6367 7438 1391 Size: 87 188 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 473 443 399 306 276 228 218 208 199 188 129 103 87 85 85 80 55 42 38 BspEI T'CCGG_A Cuts at: 3093 3151 4149 7884 3093 Size: 58 998 3735 3430 Fragments arranged by size: 3735 3430 998 58 BspHI T'CATG_A Cuts at: 2361 6110 7840 2361 Size: 3749 1730 2742 Fragments arranged by size: 3749 2742 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2815 3137 4186 4495 4675 4971 5053 Size: 1265 706 322 1049 309 180 296 82 Cuts at: 5053 5522 5576 844 Size: 469 54 3489 Fragments arranged by size: 3489 1265 1049 706 469 322 309 296 180 82 54 BsrBI CCG'CTC Cuts at: 1394 1823 3073 4638 5022 6108 1394 Size: 429 1250 1565 384 1086 3507 Fragments arranged by size: 3507 1565 1250 1086 429 384 BsrDI GCAATG_nn' Cuts at: 65 1248 2965 3820 4815 4869 6716 6890 65 Size: 1183 1717 855 995 54 1847 174 1396 Fragments arranged by size: 1847 1717 1396 1183 995 855 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 2390 2861 2901 2914 3099 4289 4478 Size: 110 580 471 40 13 185 1190 189 Cuts at: 4478 4884 5274 6865 7052 1700 Size: 406 390 1591 187 2869 Fragments arranged by size: 2869 1591 1190 580 471 406 390 189 187 185 110 40 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8221 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8221 BssSI C'ACGA_G Cuts at: 6281 7293 6281 Size: 1012 7209 BstAPI GCAn_nnn'nTGC Cuts at: 794 3138 3793 5068 5222 794 Size: 2344 655 1275 154 3793 Fragments arranged by size: 3793 2344 1275 655 154 BstBI TT'CG_AA Cuts at: 7025 7025 Size: 8221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8221 BstXI CCAn_nnnn'nTGG Cuts at: 3376 4306 4384 4433 5110 5489 3376 Size: 930 78 49 677 379 6108 Fragments arranged by size: 6108 930 677 379 78 49 BstYI r'GATC_y Cuts at: 1972 3873 3903 4006 4806 5316 5472 5551 5918 Size: 1901 30 103 800 510 156 79 367 Cuts at: 5918 6303 6320 7772 7858 7870 8216 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1901 1452 800 510 385 367 346 156 103 86 79 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3977 4313 3977 Size: 336 7885 BtgI C'CryG_G Cuts at: 513 1065 2071 3856 5361 513 Size: 552 1006 1785 1505 3373 Fragments arranged by size: 3373 1785 1505 1006 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7482 Fragments arranged by size: 7482 683 56 BtsI GCAGTG_nn' Cuts at: 2856 3168 3203 3506 4132 4935 4982 5737 6531 Size: 312 35 303 626 803 47 755 794 Cuts at: 6531 6551 2856 Size: 20 4526 Fragments arranged by size: 4526 803 794 755 626 312 303 47 35 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8221 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4348 4383 529 Size: 35 3784 35 4367 Fragments arranged by size: 4367 3784 35 35 DraI TTT'AAA Cuts at: 5525 5525 Size: 8221 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7228 809 Size: 507 5912 1802 Fragments arranged by size: 5912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3420 3427 Size: 54 1425 50 275 18 934 656 7 Cuts at: 3427 3585 3957 5072 5668 6557 8186 8 Size: 158 372 1115 596 889 1629 43 Fragments arranged by size: 1629 1425 1115 934 889 656 596 372 275 158 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 5668 1537 Size: 275 3856 4090 Fragments arranged by size: 4090 3856 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4094 6151 1353 Size: 2741 2057 3423 Fragments arranged by size: 3423 2741 2057 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2474 2933 3542 5132 6808 7182 Size: 1064 138 885 459 609 1590 1676 374 Cuts at: 7182 7328 387 Size: 146 1280 Fragments arranged by size: 1676 1590 1280 1064 885 609 459 374 146 138 EcoICRI GAG'CTC Cuts at: 5140 5140 Size: 8221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2929 3869 3974 4037 4070 4122 Size: 393 568 729 940 105 63 33 52 Cuts at: 4122 4340 4360 4526 5474 6284 6881 7668 7936 Size: 218 20 166 948 810 597 787 268 Cuts at: 7936 1239 Size: 1524 Fragments arranged by size: 1524 948 940 810 787 729 597 568 393 268 218 166 105 63 52 33 20 EcoNI CCTnn'n_nnAGG Cuts at: 4019 4967 4019 Size: 948 7273 EcoO109I rG'GnC_Cy Cuts at: 2076 2098 2115 2378 2796 2807 3315 3380 3631 Size: 22 17 263 418 11 508 65 251 Cuts at: 3631 3687 3790 3839 3994 4485 2076 Size: 56 103 49 155 491 5812 Fragments arranged by size: 5812 508 491 418 263 251 155 103 65 56 49 22 17 11 EcoRI G'AATT_C Cuts at: 5530 5908 5530 Size: 378 7843 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3268 3268 Size: 8221 FspI TGC'GCA Cuts at: 2782 6727 2782 Size: 3945 4276 HaeII r_GCGC'y Cuts at: 1678 2410 2715 2881 3106 3232 3355 3543 4612 Size: 732 305 166 225 126 123 188 1069 Cuts at: 4612 4632 7368 1678 Size: 20 2736 2531 Fragments arranged by size: 2736 2531 1069 732 305 225 188 166 126 123 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 2579 2611 3635 Size: 32 135 32 878 32 97 32 1024 Cuts at: 3635 3667 4016 4048 6862 6894 6936 6968 1373 Size: 32 349 32 2814 32 42 32 2626 Fragments arranged by size: 2814 2626 1024 878 349 135 97 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5565 5811 669 Size: 988 281 3627 246 3079 Fragments arranged by size: 3627 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4066 4301 748 Size: 3318 235 4668 Fragments arranged by size: 4668 3318 235 HpaI GTT'AAC Cuts at: 5811 5811 Size: 8221 KasI G'GCGC_C Cuts at: 3102 3102 Size: 8221 KpnI G_GTAC'C Cuts at: 5546 5546 Size: 8221 MfeI C'AATT_G Cuts at: 3037 5820 3037 Size: 2783 5438 MluI A'CGCG_T Cuts at: 8097 8097 Size: 8221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2596 4227 5376 7335 7519 1305 Size: 1291 1631 1149 1959 184 2007 Fragments arranged by size: 2007 1959 1631 1291 1149 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3422 3429 3587 3959 10 Size: 54 1425 343 1590 7 158 372 4272 Fragments arranged by size: 4272 1590 1425 372 343 158 54 7 MslI CAynn'nnrTG Cuts at: 518 1166 2766 2819 3294 5366 5487 6179 6538 Size: 648 1600 53 475 2072 121 692 359 Cuts at: 6538 6697 518 Size: 159 2042 Fragments arranged by size: 2072 2042 1600 692 648 475 359 159 121 53 MspA1I CmG'CkG Cuts at: 1606 2326 2819 2993 3082 3294 3660 3693 4003 Size: 720 493 174 89 212 366 33 310 Cuts at: 4003 4455 4519 4546 5110 5438 5637 6314 7462 Size: 452 64 27 564 328 199 677 1148 Cuts at: 7462 7707 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 720 677 564 493 452 366 328 310 245 212 199 174 89 64 33 27 NaeI GCC'GGC Cuts at: 2392 2863 2392 Size: 471 7750 NarI GG'CG_CC Cuts at: 3103 3103 Size: 8221 NcoI C'CATG_G Cuts at: 513 1065 5361 513 Size: 552 4296 3373 Fragments arranged by size: 4296 3373 552 NdeI CA'TA_TG Cuts at: 387 3019 387 Size: 2632 5589 NgoMIV G'CCGG_C Cuts at: 2390 2861 2390 Size: 471 7750 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8221 NotI GC'GGCC_GC Cuts at: 5668 5668 Size: 8221 NspI r_CATG'y Cuts at: 1132 1229 2054 2398 2979 3218 3331 5579 7124 Size: 97 825 344 581 239 113 2248 1545 Cuts at: 7124 1132 Size: 2229 Fragments arranged by size: 2248 2229 1545 825 581 344 239 113 97 PasI CC'CwG_GG Cuts at: 1348 4473 1348 Size: 3125 5096 PciI A'CATG_T Cuts at: 2975 7120 2975 Size: 4145 4076 PflMI CCAn_nnn'nTGG Cuts at: 1108 2093 2283 2449 3831 4612 4930 1108 Size: 985 190 166 1382 781 318 4399 Fragments arranged by size: 4399 1382 985 781 318 190 166 PfoI T'CCnGG_A Cuts at: 3317 3780 3869 4689 4731 5037 3317 Size: 463 89 820 42 306 6501 Fragments arranged by size: 6501 820 463 306 89 42 PmeI GTTT'AAAC Cuts at: 5525 5525 Size: 8221 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2300 2332 6290 6322 7803 7835 2300 Size: 32 3958 32 1481 32 2686 Fragments arranged by size: 3958 2686 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2115 3315 3380 2098 Size: 17 1200 65 6939 Fragments arranged by size: 6939 1200 65 17 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8221 PsiI TTA'TAA Cuts at: 5791 5791 Size: 8221 PspOMI G'GGCC_C Cuts at: 2378 3631 3686 2378 Size: 1253 55 6913 Fragments arranged by size: 6913 1253 55 PstI C_TGCA'G Cuts at: 830 2325 2745 2806 3547 4002 4460 4794 4989 Size: 1495 420 61 741 455 458 334 195 Cuts at: 4989 5573 830 Size: 584 3478 Fragments arranged by size: 3478 1495 741 584 458 455 420 334 195 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8221 PvuII CAG'CTG Cuts at: 2326 3082 4003 4519 5110 2326 Size: 756 921 516 591 5437 Fragments arranged by size: 5437 921 756 591 516 SacI G_AGCT'C Cuts at: 5142 5142 Size: 8221 SalI G'TCGA_C Cuts at: 1936 5563 1936 Size: 3627 4594 SanDI GG'GwC_CC Cuts at: 3315 3380 3315 Size: 65 8156 SbfI CC_TGCA'GG Cuts at: 5573 5573 Size: 8221 ScaI AGT'ACT Cuts at: 6469 8181 6469 Size: 1712 6509 SfcI C'TryA_G Cuts at: 826 1045 2236 2321 2741 2802 3543 3998 4456 Size: 219 1191 85 420 61 741 455 458 Cuts at: 4456 4790 4985 5569 6704 7385 7576 826 Size: 334 195 584 1135 681 191 1471 Fragments arranged by size: 1471 1191 1135 741 681 584 458 455 420 334 219 195 191 85 61 SfiI GGCCn_nnn'nGGCC Cuts at: 4484 4754 4484 Size: 270 7951 SfoI GGC'GCC Cuts at: 3104 3104 Size: 8221 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8221 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8221 SmaI CCC'GGG Cuts at: 1872 3108 3636 5548 1872 Size: 1236 528 1912 4545 Fragments arranged by size: 4545 1912 1236 528 SmlI C'TyrA_G Cuts at: 820 1017 1943 2653 6325 7226 7488 820 Size: 197 926 710 3672 901 262 1553 Fragments arranged by size: 3672 1553 926 901 710 262 197 SnaBI TAC'GTA Cuts at: 493 6012 493 Size: 5519 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8221 SphI G_CATG'C Cuts at: 1132 2054 2398 5579 1132 Size: 922 344 3181 3774 Fragments arranged by size: 3774 3181 922 344 SrfI GCCC'GGGC Cuts at: 3636 3636 Size: 8221 SspI AAT'ATT Cuts at: 5 52 6145 5 Size: 47 6093 2081 Fragments arranged by size: 6093 2081 47 StuI AGG'CCT Cuts at: 1517 2542 1517 Size: 1025 7196 StyI C'CwwG_G Cuts at: 513 1065 5361 5660 513 Size: 552 4296 299 3074 Fragments arranged by size: 4296 3074 552 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 2800 4363 5102 5288 6248 6265 Size: 1255 609 611 1563 739 186 960 17 Cuts at: 6265 6418 6603 7048 325 Size: 153 185 445 1498 Fragments arranged by size: 1563 1498 1255 960 739 611 609 445 186 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6467 8179 96 Size: 274 80 33 51 1443 4490 1712 138 Fragments arranged by size: 4490 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 3317 3349 5308 5340 8181 8213 2504 Size: 32 781 32 1959 32 2841 32 2512 Fragments arranged by size: 2841 2512 1959 781 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4030 1316 Size: 2714 5507 XbaI T'CTAG_A Cuts at: 5557 5557 Size: 8221 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3017 4840 5368 3017 Size: 1823 528 5870 Fragments arranged by size: 5870 1823 528 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8221 XmaI C'CCGG_G Cuts at: 1870 3106 3634 5546 1870 Size: 1236 528 1912 4545 Fragments arranged by size: 4545 1912 1236 528 XmnI GAAnn'nnTTC Cuts at: 6350 6350 Size: 8221 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6029 276 Size: 53 83 186 936 4495 2468 Fragments arranged by size: 4495 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SwaI