Restriction map for
pFN21AB4802
(Linear) (Six-base) MAPSORT of: KIBB4802.seq Check: 8327 from: 1 to: 3516
KIBB4802.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2490 3516
Size: 2490 1026
AccI GT'mk_AC
Cuts at: 0 1800 3516
Size: 1800 1716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2117 3516
Size: 2117 1399
AfeI AGC'GCT
Cuts at: 0 403 874 1225 1536 2605 3516
Size: 403 471 351 311 1069 911
Fragments arranged by size:
1069 911 471 403 351 311
AflIII A'CryG_T
Cuts at: 0 970 3516
Size: 970 2546
AgeI A'CCGG_T
Cuts at: 0 3269 3516
Size: 3269 247
AleI CACnn'nnGTG
Cuts at: 0 814 1289 3516
Size: 814 475 2227
Fragments arranged by size:
2227 814 475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 869 2882 3516
Size: 869 2013 634
Fragments arranged by size:
2013 869 634
AlwNI CAG_nnn'CTG
Cuts at: 0 444 525 1788 1837 1992 2779 3063 3516
Size: 444 81 1263 49 155 787 284 453
Fragments arranged by size:
1263 787 453 444 284 155 81 49
ApaI G_GGCC'C
Cuts at: 0 377 1630 1685 3516
Size: 377 1253 55 1831
Fragments arranged by size:
1831 1253 377 55
ApaLI G'TGCA_C
Cuts at: 0 878 3516
Size: 878 2638
ApoI r'AATT_y
Cuts at: 0 620 3516
Size: 620 2896
AvaI C'yCGr_G
Cuts at: 0 473 1101 1629 3516
Size: 473 628 528 1887
Fragments arranged by size:
1887 628 528 473
BamHI G'GATC_C
Cuts at: 0 1868 3311 3516
Size: 1868 1443 205
Fragments arranged by size:
1868 1443 205
BanI G'GyrC_C
Cuts at: 0 515 1097 2281 3007 3196 3304 3516
Size: 515 582 1184 726 189 108 212
Fragments arranged by size:
1184 726 582 515 212 189 108
BanII G_rGCy'C
Cuts at: 0 377 1170 1630 1685 2919 3137 3516
Size: 377 793 460 55 1234 218 379
Fragments arranged by size:
1234 793 460 379 377 218 55
BbeI G_GCGC'C
Cuts at: 0 1101 3516
Size: 1101 2415
BbsI GAAGACnn'nnnn_
Cuts at: 0 2315 3331 3516
Size: 2315 1016 185
Fragments arranged by size:
2315 1016 185
BbvCI CC'TCA_GC
Cuts at: 0 1465 2891 2897 3047 3516
Size: 1465 1426 6 150 469
Fragments arranged by size:
1465 1426 469 150 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 2557 2591 2695 2729 3516
Size: 493 34 2030 34 104 34 787
Fragments arranged by size:
2030 787 493 104 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 534 1670 1706 2479 2749 3516
Size: 534 1136 36 773 270 767
Fragments arranged by size:
1136 773 767 534 270 36
BlpI GC'TnA_GC
Cuts at: 0 2152 2186 2507 3429 3516
Size: 2152 34 321 922 87
Fragments arranged by size:
2152 922 321 87 34
Bme1580I G_kGCm'C
Cuts at: 0 377 683 882 962 1501 1630 1685 2601
Size: 377 306 199 80 539 129 55 916
Cuts at: 2601 2877 3222 3309 3516
Size: 276 345 87 207
Fragments arranged by size:
916 539 377 345 306 276 207 199
129 87 80 55
BmgBI CAC'GTC
Cuts at: 0 1578 3516
Size: 1578 1938
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 1174 3516
Size: 165 1009 2342
Fragments arranged by size:
2342 1009 165
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 423 1927 3516
Size: 423 1504 1589
Fragments arranged by size:
1589 1504 423
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 924 1864 1969 2032 2065 2335 2355
Size: 195 729 940 105 63 33 270 20
Cuts at: 2355 2521 3469 3516
Size: 166 948 47
Fragments arranged by size:
948 940 729 270 195 166 105 63
47 33 20
Bpu10I CC'TnA_GC
Cuts at: 0 1465 1946 2224 2891 2897 2990 3047 3516
Size: 1465 481 278 667 6 93 57 469
Fragments arranged by size:
1465 667 481 469 278 93 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 3516
Size: 669 2847
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 900 1114 2033 2077 2199 2605 3346
Size: 151 749 214 919 44 122 406 741
Cuts at: 3346 3516
Size: 170
Fragments arranged by size:
919 749 741 406 214 170 151 122
44
BsaHI Gr'CG_yC
Cuts at: 0 926 1098 3516
Size: 926 172 2418
Fragments arranged by size:
2418 926 172
BsaWI w'CCGG_w
Cuts at: 0 1088 1146 1562 2144 3269 3516
Size: 1088 58 416 582 1125 247
Fragments arranged by size:
1125 1088 582 416 247 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 2008 2011 2038 2041 3516
Size: 445 30 1533 3 27 3 1475
Fragments arranged by size:
1533 1475 445 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 396 1351 2029 2032 2285 2288 2291 3145
Size: 396 955 678 3 253 3 3 854
Cuts at: 3145 3352 3516
Size: 207 164
Fragments arranged by size:
955 854 678 396 253 207 164 3
3 3
BseYI C'CCAG_C
Cuts at: 0 703 725 2531 2600 3105 3179 3291 3516
Size: 703 22 1806 69 505 74 112 225
Fragments arranged by size:
1806 703 505 225 112 74 69 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 441 838 1063 1234 1551 3516
Size: 150 291 397 225 171 317 1965
Fragments arranged by size:
1965 397 317 291 225 171 150
BsiHKAI G_wGCw'C
Cuts at: 0 882 1398 2158 3137 3497 3516
Size: 882 516 760 979 360 19
Fragments arranged by size:
979 882 760 516 360 19
BsmI GAATG_Cn'
Cuts at: 0 940 3516
Size: 940 2576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1568 2014 3516
Size: 1568 446 1502
Fragments arranged by size:
1568 1502 446
Bsp1286I G_dGCh'C
Cuts at: 0 377 683 882 962 1170 1398 1501 1630
Size: 377 306 199 80 208 228 103 129
Cuts at: 1630 1685 2158 2601 2877 2919 3137 3222 3309
Size: 55 473 443 276 42 218 85 87
Cuts at: 3309 3497 3516
Size: 188 19
Fragments arranged by size:
473 443 377 306 276 228 218 208
199 188 129 103 87 85 80 55
42 19
BspEI T'CCGG_A
Cuts at: 0 1088 1146 2144 3516
Size: 1088 58 998 1372
Fragments arranged by size:
1372 1088 998 58
BspHI T'CATG_A
Cuts at: 0 356 3516
Size: 356 3160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 810 1132 2181 2490 2670 2966 3048
Size: 104 706 322 1049 309 180 296 82
Cuts at: 3048 3516
Size: 468
Fragments arranged by size:
1049 706 468 322 309 296 180 104
82
BsrBI CCG'CTC
Cuts at: 0 1068 2633 3017 3516
Size: 1068 1565 384 499
Fragments arranged by size:
1565 1068 499 384
BsrDI GCAATG_nn'
Cuts at: 0 960 1815 2810 2864 3516
Size: 960 855 995 54 652
Fragments arranged by size:
995 960 855 652 54
BsrFI r'CCGG_y
Cuts at: 0 385 856 896 909 1094 2284 2473 2879
Size: 385 471 40 13 185 1190 189 406
Cuts at: 2879 3269 3516
Size: 390 247
Fragments arranged by size:
1190 471 406 390 385 247 189 185
40 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1133 1788 3063 3217 3516
Size: 1133 655 1275 154 299
Fragments arranged by size:
1275 1133 655 299 154
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1371 2301 2379 2428 3105 3484 3516
Size: 1371 930 78 49 677 379 32
Fragments arranged by size:
1371 930 677 379 78 49 32
BstYI r'GATC_y
Cuts at: 0 1868 1898 2001 2801 3311 3467 3516
Size: 1868 30 103 800 510 156 49
Fragments arranged by size:
1868 800 510 156 103 49 30
Bsu36I CC'TnA_GG
Cuts at: 0 1972 2308 3516
Size: 1972 336 1208
Fragments arranged by size:
1972 1208 336
BtgI C'CryG_G
Cuts at: 0 66 1851 3356 3516
Size: 66 1785 1505 160
Fragments arranged by size:
1785 1505 160 66
BtsI GCAGTG_nn'
Cuts at: 0 851 1163 1198 1501 2127 2930 2977 3516
Size: 851 312 35 303 626 803 47 539
Fragments arranged by size:
851 803 626 539 312 303 47 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2343 2378 3516
Size: 2343 35 1138
Fragments arranged by size:
2343 1138 35
EaeI y'GGCC_r
Cuts at: 0 759 1415 1422 1580 1952 3067 3516
Size: 759 656 7 158 372 1115 449
Fragments arranged by size:
1115 759 656 449 372 158 7
EarI CTCTTCn'nnn_
Cuts at: 0 2089 3516
Size: 2089 1427
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 928 1537 3127 3516
Size: 469 459 609 1590 389
Fragments arranged by size:
1590 609 469 459 389
EcoICRI GAG'CTC
Cuts at: 0 3135 3516
Size: 3135 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 924 1864 1969 2032 2065 2117 2335
Size: 195 729 940 105 63 33 52 218
Cuts at: 2335 2355 2521 3469 3516
Size: 20 166 948 47
Fragments arranged by size:
948 940 729 218 195 166 105 63
52 47 33 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2014 2962 3516
Size: 2014 948 554
Fragments arranged by size:
2014 948 554
EcoO109I rG'GnC_Cy
Cuts at: 0 71 93 110 373 791 802 1310 1375
Size: 71 22 17 263 418 11 508 65
Cuts at: 1375 1626 1682 1785 1834 1989 2480 3516
Size: 251 56 103 49 155 491 1036
Fragments arranged by size:
1036 508 491 418 263 251 155 103
71 65 56 49 22 17 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1263 3516
Size: 1263 2253
FspI TGC'GCA
Cuts at: 0 777 3516
Size: 777 2739
HaeII r_GCGC'y
Cuts at: 0 405 710 876 1101 1227 1350 1538 2607
Size: 405 305 166 225 126 123 188 1069
Cuts at: 2607 2627 3516
Size: 20 889
Fragments arranged by size:
1069 889 405 305 225 188 166 126
123 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 477 574 606 1630 1662 2011 2043
Size: 445 32 97 32 1024 32 349 32
Cuts at: 2043 3516
Size: 1473
Fragments arranged by size:
1473 1024 445 349 97 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2061 2296 3516
Size: 2061 235 1220
Fragments arranged by size:
2061 1220 235
KasI G'GCGC_C
Cuts at: 0 1097 3516
Size: 1097 2419
MfeI C'AATT_G
Cuts at: 0 1032 3516
Size: 1032 2484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 2222 3371 3516
Size: 591 1631 1149 145
Fragments arranged by size:
1631 1149 591 145
MscI TGG'CCA
Cuts at: 0 1417 1424 1582 1954 3516
Size: 1417 7 158 372 1562
Fragments arranged by size:
1562 1417 372 158 7
MslI CAynn'nnrTG
Cuts at: 0 761 814 1289 3361 3482 3516
Size: 761 53 475 2072 121 34
Fragments arranged by size:
2072 761 475 121 53 34
MspA1I CmG'CkG
Cuts at: 0 321 814 988 1077 1289 1655 1688 1998
Size: 321 493 174 89 212 366 33 310
Cuts at: 1998 2450 2514 2541 3105 3433 3516
Size: 452 64 27 564 328 83
Fragments arranged by size:
564 493 452 366 328 321 310 212
174 89 83 64 33 27
NaeI GCC'GGC
Cuts at: 0 387 858 3516
Size: 387 471 2658
Fragments arranged by size:
2658 471 387
NarI GG'CG_CC
Cuts at: 0 1098 3516
Size: 1098 2418
NcoI C'CATG_G
Cuts at: 0 3356 3516
Size: 3356 160
NdeI CA'TA_TG
Cuts at: 0 1014 3516
Size: 1014 2502
NgoMIV G'CCGG_C
Cuts at: 0 385 856 3516
Size: 385 471 2660
Fragments arranged by size:
2660 471 385
NspI r_CATG'y
Cuts at: 0 49 393 974 1213 1326 3516
Size: 49 344 581 239 113 2190
Fragments arranged by size:
2190 581 344 239 113 49
PasI CC'CwG_GG
Cuts at: 0 2468 3516
Size: 2468 1048
PciI A'CATG_T
Cuts at: 0 970 3516
Size: 970 2546
PflMI CCAn_nnn'nTGG
Cuts at: 0 88 278 444 1826 2607 2925 3516
Size: 88 190 166 1382 781 318 591
Fragments arranged by size:
1382 781 591 318 190 166 88
PfoI T'CCnGG_A
Cuts at: 0 1312 1775 1864 2684 2726 3032 3516
Size: 1312 463 89 820 42 306 484
Fragments arranged by size:
1312 820 484 463 306 89 42
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 3516
Size: 295 32 3189
Fragments arranged by size:
3189 295 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 110 1310 1375 3516
Size: 93 17 1200 65 2141
Fragments arranged by size:
2141 1200 93 65 17
PspOMI G'GGCC_C
Cuts at: 0 373 1626 1681 3516
Size: 373 1253 55 1835
Fragments arranged by size:
1835 1253 373 55
PstI C_TGCA'G
Cuts at: 0 320 740 801 1542 1997 2455 2789 2984
Size: 320 420 61 741 455 458 334 195
Cuts at: 2984 3516
Size: 532
Fragments arranged by size:
741 532 458 455 420 334 320 195
61
PvuII CAG'CTG
Cuts at: 0 321 1077 1998 2514 3105 3516
Size: 321 756 921 516 591 411
Fragments arranged by size:
921 756 591 516 411 321
SacI G_AGCT'C
Cuts at: 0 3137 3516
Size: 3137 379
SanDI GG'GwC_CC
Cuts at: 0 1310 1375 3516
Size: 1310 65 2141
Fragments arranged by size:
2141 1310 65
SfcI C'TryA_G
Cuts at: 0 231 316 736 797 1538 1993 2451 2785
Size: 231 85 420 61 741 455 458 334
Cuts at: 2785 2980 3516
Size: 195 536
Fragments arranged by size:
741 536 458 455 420 334 231 195
85 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2479 2749 3516
Size: 2479 270 767
Fragments arranged by size:
2479 767 270
SfoI GGC'GCC
Cuts at: 0 1099 3516
Size: 1099 2417
SmaI CCC'GGG
Cuts at: 0 1103 1631 3516
Size: 1103 528 1885
Fragments arranged by size:
1885 1103 528
SmlI C'TyrA_G
Cuts at: 0 648 3516
Size: 648 2868
SphI G_CATG'C
Cuts at: 0 49 393 3516
Size: 49 344 3123
Fragments arranged by size:
3123 344 49
SrfI GCCC'GGGC
Cuts at: 0 1631 3516
Size: 1631 1885
StuI AGG'CCT
Cuts at: 0 537 3516
Size: 537 2979
StyI C'CwwG_G
Cuts at: 0 3356 3516
Size: 3356 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 795 2358 3097 3283 3516
Size: 184 611 1563 739 186 233
Fragments arranged by size:
1563 739 611 233 186 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1312 1344 3303 3335 3516
Size: 499 32 781 32 1959 32 181
Fragments arranged by size:
1959 781 499 181 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2025 3516
Size: 2025 1491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1012 2835 3363 3516
Size: 1012 1823 528 153
Fragments arranged by size:
1823 1012 528 153
XmaI C'CCGG_G
Cuts at: 0 1101 1629 3516
Size: 1101 528 1887
Fragments arranged by size:
1887 1101 528
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AloI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI
BsaAI BsaBI BsiEI BsiWI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspAI HincII HpaI KpnI MluI
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SspI
SwaI TatI XbaI XhoI XmnI ZraI