##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AccI        AclI        AflII       AgeI        AhdI        
AlwNI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsiWI       Bsp1407I    BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MaeI        MfeI        MluI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AatII        2750  GACGTC               GACGT^C
Acc65I        137  GGTACC               G^GTACC
AccIII       3734  TCCGGA               T^CCGGA
AflIII        152  ACGTGT               A^C[GA][CT]GT
Alw44I        581  GTGCAC               G^TGCAC
ApaLI         581  GTGCAC               G^TGCAC
Asp718I       137  GGTACC               G^GTACC
BalI         4043  TGGCCA               TGG^CCA
BamHI        2803  GGATCC               G^GATCC
BsaBI        4185  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3734  TCCGGA               T^CCGGA
BsiEI        3504  CGGTCG               CG[GA][CT]^CG
BsmI         2183  GAATGC               GAATGC(1/-1)
BspDI        4318  ATCGAT               AT^CGAT
BspEI        3734  TCCGGA               T^CCGGA
BstXI        4134  CCAGCAGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         4318  ATCGAT               AT^CGAT
DraIII       1709  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         3807  GAAGAG               CTCTTC(1/4)
EcoNI        4149  CCTCAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        4357  GAATTC               G^AATTC
KpnI          137  GGTACC               GGTAC^C
Ksp632I      3807  GAAGAG               CTCTTC(1/4)
MamI         4185  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        4043  TGGCCA               TGG^CCA
MroI         3734  TCCGGA               T^CCGGA
MscI         4043  TGGCCA               TGG^CCA
NspI         3966  GCATGT               [GA]CATG^[CT]
ScaI         3818  AGTACT               AGT^ACT
SfcI         4221  CTACAG               C^T[GA][CT]AG
SgrAI         321  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         2845  CCCGGG               CCC^GGG
StuI         4082  AGGCCT               AGG^CCT
XmaCI        2845  CCCGGG               C^CCGGG
XmaI         2845  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          521  GAATTT               [GA]^AATT[CT]
             4357  GAATTC              
Aor51HI      2599  AGCGCT               AGC^GCT
             3577  AGCGCT              
ApoI          521  GAATTT               [GA]^AATT[CT]
             4357  GAATTC              
BsgI         4242  CTGCAC               GTGCAG(16/14)
             2201  GTGCAG              
BsmBI         149  GAGACG               CGTCTC(1/5)
             3077  GAGACG              
BstEII       3100  GGTGACC              G^GT[ACGT]ACC
             3325  GGTCACC             
BstPI        3100  GGTGACC              G^GT[ACGT]ACC
             3325  GGTCACC             
DrdI         3461  GACTCAACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3551  GACCTCCTGGTC        
Eco47III     2599  AGCGCT               AGC^GCT
             3577  AGCGCT              
EcoO65I      3100  GGTGACC              G^GT[ACGT]ACC
             3325  GGTCACC             
MslI         3628  CACGATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4317  CATCGATGTG          
Tsp509I       522  AATT                 ^AATT
             4358  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AocI        ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BglI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PaeR7I      PleI        
PpuMI       PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfiI        
SfoI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XhoI        XhoII