Restriction map for
pFN21AB4786
(Linear) (Six-base) MAPSORT of: KIBB4786.seq Check: 6924 from: 1 to: 4392
KIBB4786.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 124 3781 4392
Size: 124 3657 611
Fragments arranged by size:
3657 611 124
AatII G_ACGT'C
Cuts at: 0 2754 4392
Size: 2754 1638
Acc65I G'GTAC_C
Cuts at: 0 137 4392
Size: 137 4255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 1109 2161 3576 4392
Size: 623 486 1052 1415 816
Fragments arranged by size:
1415 1052 816 623 486
AfeI AGC'GCT
Cuts at: 0 2601 3579 4392
Size: 2601 978 813
Fragments arranged by size:
2601 978 813
AflIII A'CryG_T
Cuts at: 0 152 4392
Size: 152 4240
AleI CACnn'nnGTG
Cuts at: 0 3632 4392
Size: 3632 760
ApaI G_GGCC'C
Cuts at: 0 907 998 1079 1267 3004 3491 4392
Size: 907 91 81 188 1737 487 901
Fragments arranged by size:
1737 907 901 487 188 91 81
ApaLI G'TGCA_C
Cuts at: 0 581 4392
Size: 581 3811
ApoI r'AATT_y
Cuts at: 0 521 4357 4392
Size: 521 3836 35
Fragments arranged by size:
3836 521 35
AvaI C'yCGr_G
Cuts at: 0 163 259 356 656 755 1079 1241 1412
Size: 163 96 97 300 99 324 162 171
Cuts at: 1412 2087 2845 3419 3685 4392
Size: 675 758 574 266 707
Fragments arranged by size:
758 707 675 574 324 300 266 171
163 162 99 97 96
BamHI G'GATC_C
Cuts at: 0 2803 4392
Size: 2803 1589
BanI G'GyrC_C
Cuts at: 0 137 247 823 1192 1237 1606 1714 2002
Size: 137 110 576 369 45 369 108 288
Cuts at: 2002 2137 2164 2326 2389 3055 3091 3154 3244
Size: 135 27 162 63 666 36 63 90
Cuts at: 3244 4342 4392
Size: 1098 50
Fragments arranged by size:
1098 666 576 369 369 288 162 137
135 110 108 90 63 63 50 45
36 27
BanII G_rGCy'C
Cuts at: 0 170 891 907 945 998 1079 1267 1413
Size: 170 721 16 38 53 81 188 146
Cuts at: 1413 1775 1854 2151 2385 3004 3051 3491 3742
Size: 362 79 297 234 619 47 440 251
Cuts at: 3742 4027 4177 4392
Size: 285 150 215
Fragments arranged by size:
721 619 440 362 297 285 251 234
215 188 170 150 146 81 79 53
47 38 16
BbeI G_GCGC'C
Cuts at: 0 251 2006 2168 3095 3248 4392
Size: 251 1755 162 927 153 1144
Fragments arranged by size:
1755 1144 927 251 162 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 2203 2914 3858 4392
Size: 104 2099 711 944 534
Fragments arranged by size:
2099 944 711 534 104
BglI GCCn_nnn'nGGC
Cuts at: 0 290 359 386 395 741 911 1127 1271
Size: 290 69 27 9 346 170 216 144
Cuts at: 1271 1550 1910 2432 2792 3170 3179 3242 3368
Size: 279 360 522 360 378 9 63 126
Cuts at: 3368 3746 4042 4392
Size: 378 296 350
Fragments arranged by size:
522 378 378 360 360 350 346 296
290 279 216 170 144 126 69 63
27 9 9
Bme1580I G_kGCm'C
Cuts at: 0 585 907 998 1079 1242 1267 1719 2142
Size: 585 322 91 81 163 25 452 423
Cuts at: 2142 2331 2394 3004 3159 3491 4347 4392
Size: 189 63 610 155 332 856 45
Fragments arranged by size:
856 610 585 452 423 332 322 189
163 155 91 81 63 45 25
BmgBI CAC'GTC
Cuts at: 0 153 216 3040 4392
Size: 153 63 2824 1352
Fragments arranged by size:
2824 1352 153 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 3908 4392
Size: 3908 484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 959 1409 1787 1805 1868 1937 2012
Size: 653 306 450 378 18 63 69 75
Cuts at: 2012 2039 2165 2399 2597 2984 3331 3416 4153
Size: 27 126 234 198 387 347 85 737
Cuts at: 4153 4392
Size: 239
Fragments arranged by size:
737 653 450 387 378 347 306 239
234 198 126 85 75 69 63 27
18
Bpu10I CC'TnA_GC
Cuts at: 0 1052 1340 1853 2123 3712 4392
Size: 1052 288 513 270 1589 680
Fragments arranged by size:
1589 1052 680 513 288 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2837 3599 3688 3769 4135 4392
Size: 2837 762 89 81 366 257
Fragments arranged by size:
2837 762 366 257 89 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 223 568 1936 1988 2393 3106 3475 3883
Size: 223 345 1368 52 405 713 369 408
Cuts at: 3883 4392
Size: 509
Fragments arranged by size:
1368 713 509 408 405 369 345 223
52
BsaBI GATnn'nnATC
Cuts at: 0 4189 4392
Size: 4189 203
BsaHI Gr'CG_yC
Cuts at: 0 248 326 2003 2165 2282 2751 3092 3245
Size: 248 78 1677 162 117 469 341 153
Cuts at: 3245 4392
Size: 1147
Fragments arranged by size:
1677 1147 469 341 248 162 153 117
78
BsaWI w'CCGG_w
Cuts at: 0 514 3734 4255 4392
Size: 514 3220 521 137
Fragments arranged by size:
3220 521 514 137
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1421 2915 4156 4392
Size: 22 1399 1494 1241 236
Fragments arranged by size:
1494 1399 1241 236 22
BseYI C'CCAG_C
Cuts at: 0 482 2356 3574 3605 4392
Size: 482 1874 1218 31 787
Fragments arranged by size:
1874 1218 787 482 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2222 4227 4392
Size: 2222 2005 165
Fragments arranged by size:
2222 2005 165
BsiEI CG_ry'CG
Cuts at: 0 3507 4392
Size: 3507 885
BsiHKAI G_wGCw'C
Cuts at: 0 585 756 891 963 1206 1224 1314 1413
Size: 585 171 135 72 243 18 90 99
Cuts at: 1413 1854 2106 2151 2385 2574 2592 2835 3141
Size: 441 252 45 234 189 18 243 306
Cuts at: 3141 3150 3447 4392
Size: 9 297 945
Fragments arranged by size:
945 585 441 306 297 252 243 243
234 189 171 135 99 90 72 45
18 18 9
BsmI GAATG_Cn'
Cuts at: 0 2189 4392
Size: 2189 2203
BsmBI CGTCTCn'nnnn_
Cuts at: 0 143 3071 4392
Size: 143 2928 1321
Fragments arranged by size:
2928 1321 143
Bsp1286I G_dGCh'C
Cuts at: 0 170 585 756 891 907 945 963 998
Size: 170 415 171 135 16 38 18 35
Cuts at: 998 1079 1206 1224 1242 1267 1314 1413 1719
Size: 81 127 18 18 25 47 99 306
Cuts at: 1719 1775 1854 2106 2142 2151 2331 2385 2394
Size: 56 79 252 36 9 180 54 9
Cuts at: 2394 2574 2592 2835 3004 3051 3141 3150 3159
Size: 180 18 243 169 47 90 9 9
Cuts at: 3159 3447 3491 3742 4027 4177 4347 4392
Size: 288 44 251 285 150 170 45
Fragments arranged by size:
415 306 288 285 252 251 243 180
180 171 170 170 169 150 135 127
99 90 81 79 56 54 47 47
45 44 38 36 35 25 18 18
18 18 16 9 9 9 9
BspEI T'CCGG_A
Cuts at: 0 3734 4392
Size: 3734 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 124 3781 3901 4392
Size: 124 3657 120 491
Fragments arranged by size:
3657 491 124 120
BsrFI r'CCGG_y
Cuts at: 0 322 1009 1675 2296 3115 3223 4392
Size: 322 687 666 621 819 108 1169
Fragments arranged by size:
1169 819 687 666 621 322 108
BssSI C'ACGA_G
Cuts at: 0 557 962 2384 4245 4392
Size: 557 405 1422 1861 147
Fragments arranged by size:
1861 1422 557 405 147
BstEII G'GTnAC_C
Cuts at: 0 3100 3325 4392
Size: 3100 225 1067
Fragments arranged by size:
3100 1067 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4141 4392
Size: 4141 251
BstYI r'GATC_y
Cuts at: 0 176 1561 2803 4190 4392
Size: 176 1385 1242 1387 202
Fragments arranged by size:
1387 1385 1242 202 176
Bsu36I CC'TnA_GG
Cuts at: 0 133 746 854 2222 4392
Size: 133 613 108 1368 2170
Fragments arranged by size:
2170 1368 613 133 108
BtgI C'CryG_G
Cuts at: 0 539 647 909 1476 1719 2286 2403 2727
Size: 539 108 262 567 243 567 117 324
Cuts at: 2727 2996 3105 3249 3276 3330 4392
Size: 269 109 144 27 54 1062
Fragments arranged by size:
1062 567 567 539 324 269 262 243
144 117 109 108 54 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 224 2522 4392
Size: 224 2298 1870
Fragments arranged by size:
2298 1870 224
BtsI GCAGTG_nn'
Cuts at: 0 4104 4392
Size: 4104 288
ClaI AT'CG_AT
Cuts at: 0 4319 4392
Size: 4319 73
DraIII CAC_nnn'GTG
Cuts at: 0 1714 4392
Size: 1714 2678
DrdI GACnn_nn'nnGTC
Cuts at: 0 3467 3557 4392
Size: 3467 90 835
Fragments arranged by size:
3467 835 90
EaeI y'GGCC_r
Cuts at: 0 41 63 387 2460 2640 3636 4017 4043
Size: 41 22 324 2073 180 996 381 26
Cuts at: 4043 4392
Size: 349
Fragments arranged by size:
2073 996 381 349 324 180 41 26
22
EarI CTCTTCn'nnn_
Cuts at: 0 3802 4392
Size: 3802 590
EcoICRI GAG'CTC
Cuts at: 0 889 1411 1852 2149 2383 4392
Size: 889 522 441 297 234 2009
Fragments arranged by size:
2009 889 522 441 297 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 653 959 1109 1409 1787 1805 1868
Size: 623 30 306 150 300 378 18 63
Cuts at: 1868 1937 2012 2039 2161 2165 2399 2597 2984
Size: 69 75 27 122 4 234 198 387
Cuts at: 2984 3331 3416 3576 4153 4392
Size: 347 85 160 577 239
Fragments arranged by size:
623 577 387 378 347 306 300 239
234 198 160 150 122 85 75 69
63 30 27 18 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4153 4392
Size: 4153 239
EcoO109I rG'GnC_Cy
Cuts at: 0 334 370 379 544 607 643 652 661
Size: 334 36 9 165 63 36 9 9
Cuts at: 661 670 904 995 1075 1076 1093 1246 1263
Size: 9 234 91 80 1 17 153 17
Cuts at: 1263 1264 1372 1516 1858 1903 2065 2218 2722
Size: 1 108 144 342 45 162 153 504
Cuts at: 2722 2956 3001 3271 3352 3388 3424 3488 4158
Size: 234 45 270 81 36 36 64 670
Cuts at: 4158 4392
Size: 234
Fragments arranged by size:
670 504 342 334 270 234 234 234
165 162 153 153 144 108 91 81
80 64 63 45 45 36 36 36
36 17 17 9 9 9 9 1
1
EcoRI G'AATT_C
Cuts at: 0 4357 4392
Size: 4357 35
HaeII r_GCGC'y
Cuts at: 0 251 1817 2006 2168 2603 3095 3248 3581
Size: 251 1566 189 162 435 492 153 333
Cuts at: 3581 4392
Size: 811
Fragments arranged by size:
1566 811 492 435 333 251 189 162
153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 493 525 2788 2820 3177 3209
Size: 383 32 78 32 2263 32 357 32
Cuts at: 3209 4392
Size: 1183
Fragments arranged by size:
2263 1183 383 357 78 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 247 2002 2164 3091 3244 4392
Size: 247 1755 162 927 153 1148
Fragments arranged by size:
1755 1148 927 247 162 153
KpnI G_GTAC'C
Cuts at: 0 141 4392
Size: 141 4251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4052 4202 4392
Size: 4052 150 190
Fragments arranged by size:
4052 190 150
MscI TGG'CCA
Cuts at: 0 4045 4392
Size: 4045 347
MslI CAynn'nnrTG
Cuts at: 0 3632 4321 4392
Size: 3632 689 71
Fragments arranged by size:
3632 689 71
MspA1I CmG'CkG
Cuts at: 0 486 775 1018 1298 1522 1882 2206 2360
Size: 486 289 243 280 224 360 324 154
Cuts at: 2360 2539 3124 4053 4392
Size: 179 585 929 339
Fragments arranged by size:
929 585 486 360 339 324 289 280
243 224 179 154
NarI GG'CG_CC
Cuts at: 0 248 2003 2165 3092 3245 4392
Size: 248 1755 162 927 153 1147
Fragments arranged by size:
1755 1147 927 248 162 153
NcoI C'CATG_G
Cuts at: 0 539 647 2996 4392
Size: 539 108 2349 1396
Fragments arranged by size:
2349 1396 539 108
NspI r_CATG'y
Cuts at: 0 3970 4392
Size: 3970 422
PasI CC'CwG_GG
Cuts at: 0 1098 1260 4392
Size: 1098 162 3132
Fragments arranged by size:
3132 1098 162
PfoI T'CCnGG_A
Cuts at: 0 420 447 1800 1989 2574 3546 4392
Size: 420 27 1353 189 585 972 846
Fragments arranged by size:
1353 972 846 585 420 189 27
PpuMI rG'GwC_Cy
Cuts at: 0 334 370 544 607 643 652 661 670
Size: 334 36 174 63 36 9 9 9
Cuts at: 670 1093 1372 1516 1858 2065 2722 2956 3352
Size: 423 279 144 342 207 657 234 396
Cuts at: 3352 3388 3424 4392
Size: 36 36 968
Fragments arranged by size:
968 657 423 396 342 334 279 234
207 174 144 63 36 36 36 36
9 9 9
PspOMI G'GGCC_C
Cuts at: 0 903 994 1075 1263 3000 3487 4392
Size: 903 91 81 188 1737 487 905
Fragments arranged by size:
1737 905 903 487 188 91 81
PspXI vC'TCGA_Gb
Cuts at: 0 755 1412 3685 4392
Size: 755 657 2273 707
Fragments arranged by size:
2273 755 707 657
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4084 4116 4392
Size: 4084 32 276
Fragments arranged by size:
4084 276 32
PvuII CAG'CTG
Cuts at: 0 486 775 2206 4053 4392
Size: 486 289 1431 1847 339
Fragments arranged by size:
1847 1431 486 339 289
SacI G_AGCT'C
Cuts at: 0 891 1413 1854 2151 2385 4392
Size: 891 522 441 297 234 2007
Fragments arranged by size:
2007 891 522 441 297 234
SanDI GG'GwC_CC
Cuts at: 0 334 370 544 652 1516 1858 3352 4392
Size: 334 36 174 108 864 342 1494 1040
Fragments arranged by size:
1494 1040 864 342 334 174 108 36
ScaI AGT'ACT
Cuts at: 0 3820 4392
Size: 3820 572
SexAI A'CCwGG_T
Cuts at: 0 585 711 1305 1467 1710 1971 2466 2484
Size: 585 126 594 162 243 261 495 18
Cuts at: 2484 4392
Size: 1908
Fragments arranged by size:
1908 594 585 495 261 243 162 126
18
SfcI C'TryA_G
Cuts at: 0 4221 4392
Size: 4221 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 359 386 741 911 1127 1271 1550 3170
Size: 359 27 355 170 216 144 279 1620
Cuts at: 3170 4392
Size: 1222
Fragments arranged by size:
1620 1222 359 355 279 216 170 144
27
SfoI GGC'GCC
Cuts at: 0 249 2004 2166 3093 3246 4392
Size: 249 1755 162 927 153 1146
Fragments arranged by size:
1755 1146 927 249 162 153
SgrAI Cr'CCGG_yG
Cuts at: 0 322 4392
Size: 322 4070
SmaI CCC'GGG
Cuts at: 0 2847 4392
Size: 2847 1545
SmlI C'TyrA_G
Cuts at: 0 755 1412 2852 3614 3685 3703 3784 4150
Size: 755 657 1440 762 71 18 81 366
Cuts at: 4150 4392
Size: 242
Fragments arranged by size:
1440 762 755 657 366 242 81 71
18
StuI AGG'CCT
Cuts at: 0 4084 4392
Size: 4084 308
StyI C'CwwG_G
Cuts at: 0 338 364 539 594 603 647 827 1071
Size: 338 26 175 55 9 44 180 244
Cuts at: 1071 1286 1511 1610 1998 2996 3095 3267 3837
Size: 215 225 99 388 998 99 172 570
Cuts at: 3837 3953 4331 4392
Size: 116 378 61
Fragments arranged by size:
998 570 388 378 338 244 225 215
180 175 172 116 99 99 61 55
44 26 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 161 318 1810 2638 4006 4392
Size: 161 157 1492 828 1368 386
Fragments arranged by size:
1492 1368 828 386 161 157
TatI w'GTAC_w
Cuts at: 0 3818 4392
Size: 3818 574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 544 4392
Size: 512 32 3848
Fragments arranged by size:
3848 512 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 490 610 1105 1933 2167 3274 3355 4009
Size: 490 120 495 828 234 1107 81 654
Cuts at: 4009 4320 4392
Size: 311 72
Fragments arranged by size:
1107 828 654 495 490 311 234 120
81 72
XhoI C'TCGA_G
Cuts at: 0 755 1412 3685 4392
Size: 755 657 2273 707
Fragments arranged by size:
2273 755 707 657
XmaI C'CCGG_G
Cuts at: 0 2845 4392
Size: 2845 1547
ZraI GAC'GTC
Cuts at: 0 2752 4392
Size: 2752 1640
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrFI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NspI PasI PfoI PpuMI PspOMI PspXI
PsrI PvuII SacI SanDI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AlfI AloI AlwNI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaXI
BsiWI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I CspCI DraI EagI EciI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
RsrII SacII SalI SapI SbfI SgfI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmnI