Restriction map for
pFN21AB4613
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 741 GTAC GT^AC
Alw44I 55 GTGCAC G^TGCAC
AlwNI 112 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 55 GTGCAC G^TGCAC
AspHI 55 GTGCAC G[AT]GC[AT]^C
AvaI 480 CTCGGG C^[CT]CG[GA]G
BalI 213 TGGCCA TGG^CCA
BanII 566 GGGCTC G[GA]GC[CT]^C
BbeI 727 GGCGCC GGCGC^C
BglI 215 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 463 GAGGAG GAGGAG(10/8)
BsiEI 5 CGACCG CG[GA][CT]^CG
BsiHKAI 55 GTGCAC G[AT]GC[AT]^C
BsmAI 681 GAGAC GTCTC(1/5)
BsoBI 480 CTCGGG C^[CT]CG[GA]G
BspLU11I 425 ACATGT A^CATGT
BspMI 392 GCAGGT ACCTGC(4/8)
BsrBI 160 CCGCTC CCGCTC(-3/-3)
BssHII 224 GCGCGC G^CGCGC
BssSI 492 CACGAG CACGAG(-5/-1)
DraII 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 772 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6 GACCGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 771 CCACGG C^C[GA][CT]GG
EaeI 213 TGGCCA [CT]^GGCC[GA]
EcoO109I 401 AGGCCCT [GA]G^G[ACGT]CC[CT]
KasI 727 GGCGCC G^GCGCC
MaeIII 674 GTAAC ^GT[ACGT]AC
MluNI 213 TGGCCA TGG^CCA
MscI 213 TGGCCA TGG^CCA
MslI 546 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 727 GGCGCC GG^CGCC
PshAI 783 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 741 GTAC GT^AC
SfoI 727 GGCGCC GGC^GCC
SphI 77 GCATGC GCATG^C
TaqI 548 TCGA T^CGA
TspRI 178 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 548 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 727 GGCGCC G[GA]^CG[CT]C
783 GACGCC
AflIII 368 ACGTGT A^C[GA][CT]GT
425 ACATGT
AlwI 182 GGATC GGATC(4/5)
308 GGATC
AvaII 205 GGTCC G^G[AT]CC
539 GGACC
BanI 352 GGCACC G^G[CT][GA]CC
727 GGCGCC
BlpI 162 GCTCAGC GC^T[ACGT]AGC
411 GCTGAGC
Bpu1102I 162 GCTCAGC GC^T[ACGT]AGC
411 GCTGAGC
BsaHI 727 GGCGCC G[GA]^CG[CT]C
783 GACGCC
BsgI 121 GTGCAG GTGCAG(16/14)
778 GTGCAG
BsmFI 715 GGGAC GGGAC(10/14)
755 GGGAC
BsrI 315 ACTGG ACTGG(1/-1)
177 CCAGT
BstF5I 698 GGATG GGATG(2/0)
735 GGATG
CelII 162 GCTCAGC GC^T[ACGT]AGC
411 GCTGAGC
DdeI 163 CTCAG C^T[ACGT]AG
412 CTGAG
EcoNI 117 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
706 CCTCCCGGAGG
FokI 698 GGATG GGATG(9/13)
735 GGATG
Hin1I 727 GGCGCC G[GA]^CG[CT]C
783 GACGCC
HinfI 472 GACTC G^A[ACGT]TC
717 GACTC
MaeII 335 ACGT A^CGT
368 ACGT
NlaIII 78 CATG CATG^
426 CATG
NspI 77 GCATGC [GA]CATG^[CT]
425 ACATGT
PleI 472 GACTC GAGTC(4/5)
717 GACTC
PvuII 409 CAGCTG CAG^CTG
640 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TseI