(Circular) (Six-base) MAPSORT of: pFN21AB4613.seq Check: 7146 from: 1 to: 5500 pFN21AB4613 5500 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3310 278 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2843 1937 Size: 906 4594 Acc65I G'GTAC_C Cuts at: 2821 2821 Size: 5500 AclI AA'CG_TT Cuts at: 1563 3627 4000 1563 Size: 2064 373 3063 Fragments arranged by size: 3063 2064 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 3563 4947 5215 1632 Size: 830 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1101 830 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5303 AflIII A'CryG_T Cuts at: 1203 2373 2430 4399 5376 1203 Size: 1170 57 1969 977 1327 Fragments arranged by size: 1969 1327 1170 977 57 AgeI A'CCGG_T Cuts at: 4331 4331 Size: 5500 AhdI GACnn_n'nnGTC Cuts at: 4229 4229 Size: 5500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5500 AlwNI CAG_nnn'CTG Cuts at: 2122 3243 4815 2122 Size: 1121 1572 2807 Fragments arranged by size: 2807 1572 1121 ApaLI G'TGCA_C Cuts at: 2060 3557 4713 2060 Size: 1497 1156 2847 Fragments arranged by size: 2847 1497 1156 ApoI r'AATT_y Cuts at: 1493 2809 3035 3187 5296 1493 Size: 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 AseI AT'TA_AT Cuts at: 160 1032 4054 160 Size: 872 3022 1606 Fragments arranged by size: 3022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 AvaI C'yCGr_G Cuts at: 1870 1943 2485 2825 1870 Size: 73 542 340 4545 Fragments arranged by size: 4545 542 340 73 BamHI G'GATC_C Cuts at: 2830 3197 2830 Size: 367 5133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2357 2732 2821 4276 618 Size: 325 217 627 570 375 89 1455 1842 Fragments arranged by size: 1842 1455 627 570 375 325 217 89 BanII G_rGCy'C Cuts at: 1391 2575 2819 1391 Size: 1184 244 4072 Fragments arranged by size: 4072 1184 244 BbeI G_GCGC'C Cuts at: 2736 2736 Size: 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3691 3725 1903 Size: 34 1754 34 3678 Fragments arranged by size: 3678 1754 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3392 4602 3392 Size: 1210 4290 BclI T'GATC_A Cuts at: 1548 1548 Size: 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2226 4111 136 Size: 107 122 71 631 605 554 1885 1525 Fragments arranged by size: 1885 1525 631 605 554 122 107 71 BglII A'GATC_T Cuts at: 5495 5495 Size: 5500 BlpI GC'TnA_GC Cuts at: 2168 2417 2917 2168 Size: 249 500 4751 Fragments arranged by size: 4751 500 249 Bme1580I G_kGCm'C Cuts at: 1790 2064 2360 3561 4717 1790 Size: 274 296 1201 1156 2573 Fragments arranged by size: 2573 1201 1156 296 274 BmgBI CAC'GTC Cuts at: 1318 2341 1318 Size: 1023 4477 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2330 3231 4189 Size: 416 53 83 399 24 913 901 958 Cuts at: 4189 442 Size: 1753 Fragments arranged by size: 1753 958 913 901 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2494 2641 2494 Size: 147 5353 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 2300 2597 2669 4160 1239 Size: 790 271 297 72 1491 2579 Fragments arranged by size: 2579 1491 790 297 271 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3625 4490 4788 3625 Size: 865 298 4337 Fragments arranged by size: 4337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4163 882 Size: 617 2664 2219 Fragments arranged by size: 2664 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3291 493 Size: 711 2087 2702 Fragments arranged by size: 2702 2087 711 BsaBI GATnn'nnATC Cuts at: 5494 5494 Size: 5500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2733 2789 3307 3689 Size: 53 83 186 936 1200 56 518 382 Cuts at: 3689 4324 275 Size: 635 1451 Fragments arranged by size: 1451 1200 936 635 518 382 186 83 56 53 BsaWI w'CCGG_w Cuts at: 3933 4331 4605 4752 5163 3933 Size: 398 274 147 411 4270 Fragments arranged by size: 4270 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5275 Fragments arranged by size: 5275 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 1186 Size: 1297 4203 BseYI C'CCAG_C Cuts at: 4703 5199 4703 Size: 496 5004 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2804 1163 Size: 573 411 657 3859 Fragments arranged by size: 3859 657 573 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 2950 3711 4739 1540 Size: 275 186 12 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2064 3561 3646 4717 2064 Size: 1497 85 1071 2847 Fragments arranged by size: 2847 1497 1071 85 BsmI GAATG_Cn' Cuts at: 3011 3104 3011 Size: 93 5407 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2360 2575 2819 3561 3646 4717 Size: 399 274 296 215 244 742 85 1071 Cuts at: 4717 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 296 274 244 215 85 BspEI T'CCGG_A Cuts at: 5163 5163 Size: 5500 BspHI T'CATG_A Cuts at: 3389 5119 3389 Size: 1730 3770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2855 844 Size: 1544 467 3489 Fragments arranged by size: 3489 1544 467 BsrBI CCG'CTC Cuts at: 1394 1823 2167 3387 1394 Size: 429 344 1220 3507 Fragments arranged by size: 3507 1220 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 3995 4169 65 Size: 1183 2747 174 1396 Fragments arranged by size: 2747 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4144 4331 1700 Size: 110 2334 187 2869 Fragments arranged by size: 2869 2334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5500 BssHII G'CGCG_C Cuts at: 1925 2229 1925 Size: 304 5196 BssSI C'ACGA_G Cuts at: 2497 3560 4572 2497 Size: 1063 1012 3425 Fragments arranged by size: 3425 1063 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5500 BstBI TT'CG_AA Cuts at: 4304 4304 Size: 5500 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5500 BstYI r'GATC_y Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495 Size: 858 367 385 17 1452 86 12 346 Cuts at: 5495 1972 Size: 1977 Fragments arranged by size: 1977 1452 858 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2776 513 Size: 552 939 772 3237 Fragments arranged by size: 3237 939 772 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4761 Fragments arranged by size: 4761 683 56 BtsI GCAGTG_nn' Cuts at: 3016 3810 3830 3016 Size: 794 20 4686 Fragments arranged by size: 4686 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5465 DraI TTT'AAA Cuts at: 2804 2804 Size: 5500 DraIII CAC_nnn'GTG Cuts at: 2782 2782 Size: 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2017 4507 809 Size: 507 701 2490 1802 Fragments arranged by size: 2490 1802 701 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2947 3836 Size: 54 1425 50 275 18 388 729 889 Cuts at: 3836 5465 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 729 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2947 1537 Size: 275 1135 4090 Fragments arranged by size: 4090 1135 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3430 1353 Size: 2077 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2202 2326 2489 4087 4461 4607 Size: 1064 138 613 124 163 1598 374 146 Cuts at: 4607 387 Size: 1280 Fragments arranged by size: 1598 1280 1064 613 374 163 146 138 124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2300 2462 2597 2669 3563 4160 Size: 393 397 271 162 135 72 894 597 Cuts at: 4160 4947 5215 1239 Size: 787 268 1524 Fragments arranged by size: 1524 894 787 597 397 393 271 268 162 135 72 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2715 2126 Size: 589 4911 EcoO109I rG'GnC_Cy Cuts at: 2407 2407 Size: 5500 EcoRI G'AATT_C Cuts at: 2809 3187 2809 Size: 378 5122 FspI TGC'GCA Cuts at: 4006 4006 Size: 5500 HaeII r_GCGC'y Cuts at: 1678 2052 2145 2736 4647 1678 Size: 374 93 591 1911 2531 Fragments arranged by size: 2531 1911 591 374 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2151 2183 2231 2263 2468 Size: 32 135 32 579 32 48 32 205 Cuts at: 2468 2500 2695 2727 4141 4173 4215 4247 1373 Size: 32 195 32 1414 32 42 32 2626 Fragments arranged by size: 2626 1414 579 205 195 135 48 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2844 3090 669 Size: 988 281 906 246 3079 Fragments arranged by size: 3079 988 906 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5500 HpaI GTT'AAC Cuts at: 3090 3090 Size: 5500 KasI G'GCGC_C Cuts at: 2732 2732 Size: 5500 KpnI G_GTAC'C Cuts at: 2825 2825 Size: 5500 MfeI C'AATT_G Cuts at: 3099 3099 Size: 5500 MluI A'CGCG_T Cuts at: 5376 5376 Size: 5500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2445 4614 4798 1305 Size: 1140 2169 184 2007 Fragments arranged by size: 2169 2007 1140 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2220 10 Size: 54 1425 343 388 3290 Fragments arranged by size: 3290 1425 388 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2555 3458 3817 3976 518 Size: 648 1389 903 359 159 2042 Fragments arranged by size: 2042 1389 903 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2416 2647 2916 3593 4741 4986 1606 Size: 702 108 231 269 677 1148 245 2120 Fragments arranged by size: 2120 1148 702 677 269 245 231 108 NarI GG'CG_CC Cuts at: 2733 2733 Size: 5500 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4009 Fragments arranged by size: 4009 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5500 NotI GC'GGCC_GC Cuts at: 2947 2947 Size: 5500 NspI r_CATG'y Cuts at: 1132 1229 2086 2434 2858 4403 1132 Size: 97 857 348 424 1545 2229 Fragments arranged by size: 2229 1545 857 424 348 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5500 PciI A'CATG_T Cuts at: 2430 4399 2430 Size: 1969 3531 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5500 PfoI T'CCnGG_A Cuts at: 2713 2713 Size: 5500 PmeI GTTT'AAAC Cuts at: 2804 2804 Size: 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 5082 5114 3569 Size: 32 1481 32 3955 Fragments arranged by size: 3955 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2792 1613 Size: 1179 4321 PsiI TTA'TAA Cuts at: 3070 3070 Size: 5500 PstI C_TGCA'G Cuts at: 830 2151 2399 2415 2852 830 Size: 1321 248 16 437 3478 Fragments arranged by size: 3478 1321 437 248 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5500 PvuII CAG'CTG Cuts at: 2416 2647 2416 Size: 231 5269 SalI G'TCGA_C Cuts at: 1936 2842 1936 Size: 906 4594 SbfI CC_TGCA'GG Cuts at: 2852 2852 Size: 5500 ScaI AGT'ACT Cuts at: 3748 5460 3748 Size: 1712 3788 SfcI C'TryA_G Cuts at: 826 1045 2029 2147 2395 2411 2848 3983 4664 Size: 219 984 118 248 16 437 1135 681 Cuts at: 4664 4855 826 Size: 191 1471 Fragments arranged by size: 1471 1135 984 681 437 248 219 191 118 16 SfoI GGC'GCC Cuts at: 2734 2734 Size: 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5500 SmaI CCC'GGG Cuts at: 1872 2827 1872 Size: 955 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3604 4505 4767 820 Size: 197 926 1661 901 262 1553 Fragments arranged by size: 1661 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3291 493 Size: 2798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5500 SphI G_CATG'C Cuts at: 1132 2086 2858 1132 Size: 954 772 3774 Fragments arranged by size: 3774 954 772 SspI AAT'ATT Cuts at: 5 52 3424 5 Size: 47 3372 2081 Fragments arranged by size: 3372 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5500 StyI C'CwwG_G Cuts at: 513 1065 2004 2939 513 Size: 552 939 935 3074 Fragments arranged by size: 3074 939 935 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2238 3527 3544 3697 3882 4327 325 Size: 1255 658 1289 17 153 185 445 1498 Fragments arranged by size: 1498 1289 1255 658 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3746 5458 96 Size: 274 80 33 51 1443 1769 1712 138 Fragments arranged by size: 1769 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 5468 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5500 XbaI T'CTAG_A Cuts at: 2836 2836 Size: 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5500 XmaI C'CCGG_G Cuts at: 1870 2825 1870 Size: 955 4545 XmnI GAAnn'nnTTC Cuts at: 3629 3629 Size: 5500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3308 276 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI