Restriction map for
pFN21AB4592
(Linear) (Six-base) MAPSORT of: KIBB4592.seq Check: 2101 from: 1 to: 1803
KIBB4592.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1093 1400 1803
Size: 1093 307 403
Fragments arranged by size:
1093 403 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 944 1803
Size: 659 285 859
Fragments arranged by size:
859 659 285
AflIII A'CryG_T
Cuts at: 0 1053 1803
Size: 1053 750
AlwNI CAG_nnn'CTG
Cuts at: 0 940 1803
Size: 940 863
ApaI G_GGCC'C
Cuts at: 0 1470 1803
Size: 1470 333
ApoI r'AATT_y
Cuts at: 0 258 607 1010 1345 1365 1803
Size: 258 349 403 335 20 438
Fragments arranged by size:
438 403 349 335 258 20
AseI AT'TA_AT
Cuts at: 0 1550 1803
Size: 1550 253
AvaI C'yCGr_G
Cuts at: 0 19 1803
Size: 19 1784
BanII G_rGCy'C
Cuts at: 0 20 95 845 1470 1803
Size: 20 75 750 625 333
Fragments arranged by size:
750 625 333 75 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 1754 1803
Size: 110 1644 49
Fragments arranged by size:
1644 110 49
BciVI GTATCCnnnnn_n'
Cuts at: 0 1047 1803
Size: 1047 756
BglII A'GATC_T
Cuts at: 0 377 1803
Size: 377 1426
BlpI GC'TnA_GC
Cuts at: 0 245 438 1803
Size: 245 193 1365
Fragments arranged by size:
1365 245 193
Bme1580I G_kGCm'C
Cuts at: 0 1470 1803
Size: 1470 333
BmgBI CAC'GTC
Cuts at: 0 108 583 1803
Size: 108 475 1220
Fragments arranged by size:
1220 475 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 726 1803
Size: 726 1077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1803
Size: 829 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1803
Size: 775 1028
BsaI GGTCTCn'nnnn_
Cuts at: 0 985 1619 1803
Size: 985 634 184
Fragments arranged by size:
985 634 184
BsaBI GATnn'nnATC
Cuts at: 0 1046 1222 1803
Size: 1046 176 581
Fragments arranged by size:
1046 581 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1616 1646 1803
Size: 1616 30 157
Fragments arranged by size:
1616 157 30
BseYI C'CCAG_C
Cuts at: 0 252 1335 1803
Size: 252 1083 468
Fragments arranged by size:
1083 468 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1803
Size: 130 1673
BsiEI CG_ry'CG
Cuts at: 0 74 1803
Size: 74 1729
BsiHKAI G_wGCw'C
Cuts at: 0 20 95 559 845 1803
Size: 20 75 464 286 958
Fragments arranged by size:
958 464 286 75 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 95 559 845 1470 1803
Size: 20 75 464 286 625 333
Fragments arranged by size:
625 464 333 286 75 20
BspHI T'CATG_A
Cuts at: 0 1433 1803
Size: 1433 370
BsrDI GCAATG_nn'
Cuts at: 0 105 1803
Size: 105 1698
BsrFI r'CCGG_y
Cuts at: 0 25 1803
Size: 25 1778
BssSI C'ACGA_G
Cuts at: 0 1387 1803
Size: 1387 416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 514 940 1276 1599 1803
Size: 514 426 336 323 204
Fragments arranged by size:
514 426 336 323 204
BstYI r'GATC_y
Cuts at: 0 377 1803
Size: 377 1426
BstZ17I GTA'TAC
Cuts at: 0 1401 1803
Size: 1401 402
BtgI C'CryG_G
Cuts at: 0 854 1803
Size: 854 949
DrdI GACnn_nn'nnGTC
Cuts at: 0 1756 1803
Size: 1756 47
EaeI y'GGCC_r
Cuts at: 0 1298 1655 1803
Size: 1298 357 148
Fragments arranged by size:
1298 357 148
EarI CTCTTCn'nnn_
Cuts at: 0 329 1803
Size: 329 1474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1282 1803
Size: 1282 521
EcoICRI GAG'CTC
Cuts at: 0 18 93 843 1803
Size: 18 75 750 960
Fragments arranged by size:
960 750 75 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 829 944 1803
Size: 659 170 115 859
Fragments arranged by size:
859 659 170 115
EcoO109I rG'GnC_Cy
Cuts at: 0 173 280 859 1803
Size: 173 107 579 944
Fragments arranged by size:
944 579 173 107
EcoRI G'AATT_C
Cuts at: 0 1345 1365 1803
Size: 1345 20 438
Fragments arranged by size:
1345 438 20
HindIII A'AGCT_T
Cuts at: 0 410 713 1803
Size: 410 303 1090
Fragments arranged by size:
1090 410 303
MfeI C'AATT_G
Cuts at: 0 146 1803
Size: 146 1657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1803
Size: 141 1662
MscI TGG'CCA
Cuts at: 0 1300 1657 1803
Size: 1300 357 146
Fragments arranged by size:
1300 357 146
MslI CAynn'nnrTG
Cuts at: 0 474 571 1000 1803
Size: 474 97 429 803
Fragments arranged by size:
803 474 429 97
MspA1I CmG'CkG
Cuts at: 0 40 252 937 1320 1335 1803
Size: 40 212 685 383 15 468
Fragments arranged by size:
685 468 383 212 40 15
NcoI C'CATG_G
Cuts at: 0 854 1803
Size: 854 949
NspI r_CATG'y
Cuts at: 0 479 1057 1803
Size: 479 578 746
Fragments arranged by size:
746 578 479
PasI CC'CwG_GG
Cuts at: 0 503 1803
Size: 503 1300
PciI A'CATG_T
Cuts at: 0 1053 1803
Size: 1053 750
PpuMI rG'GwC_Cy
Cuts at: 0 173 280 859 1803
Size: 173 107 579 944
Fragments arranged by size:
944 579 173 107
PspOMI G'GGCC_C
Cuts at: 0 1466 1803
Size: 1466 337
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1115 1147 1625 1657 1803
Size: 1115 32 478 32 146
Fragments arranged by size:
1115 478 146 32 32
PvuI CG_AT'CG
Cuts at: 0 74 1803
Size: 74 1729
PvuII CAG'CTG
Cuts at: 0 252 937 1335 1803
Size: 252 685 398 468
Fragments arranged by size:
685 468 398 252
SacI G_AGCT'C
Cuts at: 0 20 95 845 1803
Size: 20 75 750 958
Fragments arranged by size:
958 750 75 20
ScaI AGT'ACT
Cuts at: 0 1724 1803
Size: 1724 79
SfcI C'TryA_G
Cuts at: 0 1726 1803
Size: 1726 77
SmlI C'TyrA_G
Cuts at: 0 754 1803
Size: 754 1049
SpeI A'CTAG_T
Cuts at: 0 664 1440 1803
Size: 664 776 363
Fragments arranged by size:
776 664 363
StuI AGG'CCT
Cuts at: 0 1567 1803
Size: 1567 236
StyI C'CwwG_G
Cuts at: 0 854 1803
Size: 854 949
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 1327 1803
Size: 592 735 476
Fragments arranged by size:
735 592 476
TatI w'GTAC_w
Cuts at: 0 1722 1803
Size: 1722 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 165 197 1803
Size: 165 32 1606
Fragments arranged by size:
1606 165 32
XbaI T'CTAG_A
Cuts at: 0 1686 1803
Size: 1686 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1263 1803
Size: 1263 540
XmnI GAAnn'nnTTC
Cuts at: 0 650 1127 1803
Size: 650 477 676
Fragments arranged by size:
676 650 477
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI AseI AvaI
BanII BbsI BciVI BglII BlpI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssSI BstAPI BstYI
BstZ17I BtgI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HindIII MfeI MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PpuMI PspOMI PsrI PvuI
PvuII SacI ScaI SfcI SmlI SpeI StuI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI
BmtI BplI Bpu10I BsaAI BsaHI BsaWI BseRI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BstBI
BstEII BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoNI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PstI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI SwaI Tth111I XhoI XmaI ZraI