(Circular) (Six-base) MAPSORT of: pFN21AB4158.seq Check: 7105 from: 1 to: 6181 pFN21AB4158 6181 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2353 2868 3524 1937 Size: 416 515 656 4594 Fragments arranged by size: 4594 656 515 416 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 2827 4308 4681 1563 Size: 1264 1481 373 3063 Fragments arranged by size: 3063 1481 1264 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2435 2754 2825 3213 4244 5628 5896 1632 Size: 803 319 71 388 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 803 388 319 268 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3389 1184 Size: 2205 3976 AlwNI CAG_nnn'CTG Cuts at: 3924 5496 3924 Size: 1572 4609 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2103 2425 2882 2941 3490 3716 3868 5977 Size: 610 322 457 59 549 226 152 2109 Cuts at: 5977 1493 Size: 1697 Fragments arranged by size: 2109 1697 610 549 457 322 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 3454 3506 1870 Size: 73 1511 52 4545 Fragments arranged by size: 4545 1511 73 52 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2909 3411 3502 4957 618 Size: 325 217 627 1122 502 91 1455 1842 Fragments arranged by size: 1842 1455 1122 627 502 325 217 91 BanII G_rGCy'C Cuts at: 1391 2994 3500 1391 Size: 1603 506 4072 Fragments arranged by size: 4072 1603 506 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2310 2661 928 Size: 971 411 351 4448 Fragments arranged by size: 4448 971 411 351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2646 4073 5283 2646 Size: 1427 1210 3544 Fragments arranged by size: 3544 1427 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 4792 136 Size: 107 122 71 631 605 1126 1994 1525 Fragments arranged by size: 1994 1525 1126 631 605 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 2482 3598 2482 Size: 1116 5065 Bme1580I G_kGCm'C Cuts at: 1790 4242 5398 1790 Size: 2452 1156 2573 Fragments arranged by size: 2573 2452 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3912 4870 442 Size: 416 53 83 399 24 2495 958 1753 Fragments arranged by size: 2495 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2442 1056 Size: 1386 4795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2535 2709 4841 1239 Size: 1068 228 174 2132 2579 Fragments arranged by size: 2579 2132 1068 228 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2564 3972 493 Size: 711 1360 1408 2702 Fragments arranged by size: 2702 1408 1360 711 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2445 2475 2713 2743 1178 Size: 30 165 30 1042 30 238 30 4616 Fragments arranged by size: 4616 1042 238 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 1186 Size: 886 5295 BseYI C'CCAG_C Cuts at: 5384 5880 5384 Size: 496 5685 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2008 1163 Size: 573 272 5336 Fragments arranged by size: 5336 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4242 4327 5398 4242 Size: 85 1071 5025 Fragments arranged by size: 5025 1071 85 BsmI GAATG_Cn' Cuts at: 2075 3378 3692 3785 2075 Size: 1303 314 93 4471 Fragments arranged by size: 4471 1303 314 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2994 3500 4242 4327 5398 1391 Size: 399 1204 506 742 85 1071 2174 Fragments arranged by size: 2174 1204 1071 742 506 399 85 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 2233 4070 5800 2233 Size: 1837 1730 2614 Fragments arranged by size: 2614 1837 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 3536 844 Size: 1186 1506 3489 Fragments arranged by size: 3489 1506 1186 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4825 5012 1700 Size: 110 3015 187 2869 Fragments arranged by size: 3015 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3301 96 Size: 3205 2976 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 3402 4241 5253 3402 Size: 839 1012 4330 Fragments arranged by size: 4330 1012 839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 3459 1184 Size: 2275 3906 BstXI CCAn_nnnn'nTGG Cuts at: 2646 2646 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2569 2817 3511 3878 4263 4280 5732 5818 Size: 597 248 694 367 385 17 1452 86 Cuts at: 5818 5830 6176 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 694 597 385 367 346 248 86 17 12 BstZ17I GTA'TAC Cuts at: 2354 2354 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 2813 513 Size: 552 939 809 3881 Fragments arranged by size: 3881 939 809 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3434 487 Size: 683 56 2208 3234 Fragments arranged by size: 3234 2208 683 56 BtsI GCAGTG_nn' Cuts at: 3373 3697 4491 4511 3373 Size: 324 794 20 5043 Fragments arranged by size: 5043 794 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3388 5188 809 Size: 507 2072 1800 1802 Fragments arranged by size: 2072 1802 1800 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3628 4517 6146 Size: 54 1425 50 275 18 1798 889 1629 Cuts at: 6146 8 Size: 43 Fragments arranged by size: 1798 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 3390 4111 1353 Size: 891 1146 721 3423 Fragments arranged by size: 3423 1146 891 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2435 2535 2709 2754 2825 3213 Size: 393 675 128 100 174 45 71 388 Cuts at: 3213 4244 4841 5628 5896 1239 Size: 1031 597 787 268 1524 Fragments arranged by size: 1524 1031 787 675 597 393 388 268 174 128 100 71 45 EcoNI CCTnn'n_nnAGG Cuts at: 2026 2026 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 2410 2410 Size: 6181 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 EcoRV GAT'ATC Cuts at: 2543 2543 Size: 6181 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 5328 1678 Size: 3650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2690 2722 4822 Size: 32 135 32 656 32 430 32 2100 Cuts at: 4822 4854 4896 4928 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2100 656 430 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2825 3036 3525 3771 669 Size: 988 281 887 211 489 246 3079 Fragments arranged by size: 3079 988 887 489 281 246 211 HindIII A'AGCT_T Cuts at: 748 2150 3195 748 Size: 1402 1045 3734 Fragments arranged by size: 3734 1402 1045 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3057 3780 3057 Size: 723 5458 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4359 Fragments arranged by size: 4359 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2391 2713 4139 4498 4657 518 Size: 648 1225 322 1426 359 159 2042 Fragments arranged by size: 2042 1426 1225 648 359 322 159 MspA1I CmG'CkG Cuts at: 1606 2459 3597 4274 5422 5667 1606 Size: 853 1138 677 1148 245 2120 Fragments arranged by size: 2120 1148 1138 853 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2813 513 Size: 552 939 809 3881 Fragments arranged by size: 3881 939 809 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NheI G'CTAG_C Cuts at: 1052 2438 1052 Size: 1386 4795 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 2604 3539 5084 1132 Size: 97 1375 935 1545 2229 Fragments arranged by size: 2229 1545 1375 935 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2410 Size: 6181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PstI C_TGCA'G Cuts at: 830 3533 830 Size: 2703 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2459 2459 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SexAI A'CCwGG_T Cuts at: 2027 2027 Size: 6181 SfcI C'TryA_G Cuts at: 826 1045 2326 2865 3529 4664 5345 5536 826 Size: 219 1281 539 664 1135 681 191 1471 Fragments arranged by size: 1471 1281 1135 681 664 539 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4285 5186 5448 820 Size: 197 926 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2564 3972 493 Size: 2071 1408 2702 Fragments arranged by size: 2702 2071 1408 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 2221 4105 5 Size: 47 2169 1884 2081 Fragments arranged by size: 2169 2081 1884 47 StuI AGG'CCT Cuts at: 1517 3174 1517 Size: 1657 4524 StyI C'CwwG_G Cuts at: 513 1065 2004 2448 2813 3620 513 Size: 552 939 444 365 807 3074 Fragments arranged by size: 3074 939 807 552 444 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2826 4208 4225 4378 4563 5008 325 Size: 1255 1246 1382 17 153 185 445 1498 Fragments arranged by size: 1498 1382 1255 1246 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3301 3354 4427 Size: 274 80 33 51 1443 1324 53 1073 Cuts at: 4427 6139 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1324 1073 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2445 2477 2571 2603 2713 2745 3413 3445 6141 Size: 32 94 32 110 32 668 32 2696 Cuts at: 6141 6173 2445 Size: 32 2453 Fragments arranged by size: 2696 2453 668 110 94 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 2365 3517 2365 Size: 1152 5029 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2592 2712 3017 2592 Size: 120 305 5756 Fragments arranged by size: 5756 305 120 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI