Restriction map for
pFN21AB4158
(Linear) (Six-base) MAPSORT of: KIBB4158.seq Check: 1471 from: 1 to: 1476
KIBB4158.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 348 863 1476
Size: 348 515 613
Fragments arranged by size:
613 515 348
AclI AA'CG_TT
Cuts at: 0 822 1476
Size: 822 654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 749 820 1208 1476
Size: 430 319 71 388 268
Fragments arranged by size:
430 388 319 268 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1384 1476
Size: 1384 92
ApoI r'AATT_y
Cuts at: 0 98 420 877 936 1476
Size: 98 322 457 59 540
Fragments arranged by size:
540 457 322 98 59
AvaI C'yCGr_G
Cuts at: 0 1449 1476
Size: 1449 27
BanI G'GyrC_C
Cuts at: 0 904 1406 1476
Size: 904 502 70
Fragments arranged by size:
904 502 70
BanII G_rGCy'C
Cuts at: 0 989 1476
Size: 989 487
BbsI GAAGACnn'nnnn_
Cuts at: 0 305 656 1476
Size: 305 351 820
Fragments arranged by size:
820 351 305
BciVI GTATCCnnnnn_n'
Cuts at: 0 641 1476
Size: 641 835
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1476
Size: 793 683
BlpI GC'TnA_GC
Cuts at: 0 477 1476
Size: 477 999
BmtI G_CTAG'C
Cuts at: 0 437 1476
Size: 437 1039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 530 704 1476
Size: 302 228 174 772
Fragments arranged by size:
772 302 228 174
BsaAI yAC'GTr
Cuts at: 0 559 1476
Size: 559 917
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 440 470 708 738 1476
Size: 440 30 238 30 738
Fragments arranged by size:
738 440 238 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1476
Size: 67 1409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1476
Size: 3 1473
BsmI GAATG_Cn'
Cuts at: 0 70 1373 1476
Size: 70 1303 103
Fragments arranged by size:
1303 103 70
Bsp1286I G_dGCh'C
Cuts at: 0 989 1476
Size: 989 487
BspHI T'CATG_A
Cuts at: 0 228 1476
Size: 228 1248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 25 1476
Size: 25 1451
BsrGI T'GTAC_A
Cuts at: 0 1296 1476
Size: 1296 180
BssSI C'ACGA_G
Cuts at: 0 1397 1476
Size: 1397 79
BstEII G'GTnAC_C
Cuts at: 0 1454 1476
Size: 1454 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 641 1476
Size: 641 835
BstYI r'GATC_y
Cuts at: 0 564 812 1476
Size: 564 248 664
Fragments arranged by size:
664 564 248
BstZ17I GTA'TAC
Cuts at: 0 349 1476
Size: 349 1127
BtgI C'CryG_G
Cuts at: 0 808 1476
Size: 808 668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1429 1476
Size: 1429 47
BtsI GCAGTG_nn'
Cuts at: 0 1368 1476
Size: 1368 108
DrdI GACnn_nn'nnGTC
Cuts at: 0 1383 1476
Size: 1383 93
EarI CTCTTCn'nnn_
Cuts at: 0 239 1385 1476
Size: 239 1146 91
Fragments arranged by size:
1146 239 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 430 530 704 749 820 1208 1476
Size: 302 128 100 174 45 71 388 268
Fragments arranged by size:
388 302 268 174 128 100 71 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 21 1476
Size: 21 1455
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1476
Size: 405 1071
EcoRV GAT'ATC
Cuts at: 0 538 1476
Size: 538 938
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 685 717 1476
Size: 223 32 430 32 759
Fragments arranged by size:
759 430 223 32 32
HincII GTy'rAC
Cuts at: 0 820 1031 1476
Size: 820 211 445
Fragments arranged by size:
820 445 211
HindIII A'AGCT_T
Cuts at: 0 145 1190 1476
Size: 145 1045 286
Fragments arranged by size:
1045 286 145
MfeI C'AATT_G
Cuts at: 0 1052 1476
Size: 1052 424
MslI CAynn'nnrTG
Cuts at: 0 386 708 1476
Size: 386 322 768
Fragments arranged by size:
768 386 322
MspA1I CmG'CkG
Cuts at: 0 454 1476
Size: 454 1022
NcoI C'CATG_G
Cuts at: 0 808 1476
Size: 808 668
NheI G'CTAG_C
Cuts at: 0 433 1476
Size: 433 1043
NspI r_CATG'y
Cuts at: 0 599 1476
Size: 599 877
PpuMI rG'GwC_Cy
Cuts at: 0 405 1476
Size: 405 1071
PvuII CAG'CTG
Cuts at: 0 454 1476
Size: 454 1022
SexAI A'CCwGG_T
Cuts at: 0 22 1476
Size: 22 1454
SfcI C'TryA_G
Cuts at: 0 321 860 1476
Size: 321 539 616
Fragments arranged by size:
616 539 321
SnaBI TAC'GTA
Cuts at: 0 559 1476
Size: 559 917
SspI AAT'ATT
Cuts at: 0 216 1476
Size: 216 1260
StuI AGG'CCT
Cuts at: 0 1169 1476
Size: 1169 307
StyI C'CwwG_G
Cuts at: 0 443 808 1476
Size: 443 365 668
Fragments arranged by size:
668 443 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 821 1476
Size: 821 655
TatI w'GTAC_w
Cuts at: 0 1296 1349 1476
Size: 1296 53 127
Fragments arranged by size:
1296 127 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 440 472 566 598 708 740 1408 1440
Size: 440 32 94 32 110 32 668 32
Cuts at: 1440 1476
Size: 36
Fragments arranged by size:
668 440 110 94 36 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 360 1476
Size: 360 1116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 587 707 1012 1476
Size: 587 120 305 464
Fragments arranged by size:
587 464 305 120
Enzymes that do cut:
AccI AclI AcuI AlfI ApoI AvaI BanI BanII
BbsI BciVI BglI BlpI BmtI BpmI BsaAI BsaXI
BseRI BsgI BsmI Bsp1286I BspHI BspMI BsrGI BssSI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DrdI
EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII HindIII
MfeI MslI MspA1I NcoI NheI NspI PpuMI PvuII
SexAI SfcI SnaBI SspI StuI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BstAPI BstBI Bsu36I
ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI
EcoRI FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI SwaI Tth111I XhoI XmaI XmnI
ZraI