Restriction map for
pFN21AB4121
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# NO CUT ENZYMES ( 119 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BpuAI
BsaAI BsaI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MfeI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 63 GACGTC GACGT^C
AccI 1026 GTCTAC GT^[AC][GT]AC
AccIII 638 TCCGGA T^CCGGA
AcyI 63 GACGTC G[GA]^CG[CT]C
AflIII 419 ACGCGT A^C[GA][CT]GT
AocI 354 CCTGAGG CC^T[ACGT]AGG
AseI 562 ATTAAT AT^TAAT
AsnI 562 ATTAAT AT^TAAT
AvaII 659 GGTCC G^G[AT]CC
BfaI 325 CTAG C^TAG
BglI 859 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 87 AGATCT A^GATCT
BlpI 1147 GCTCAGC GC^T[ACGT]AGC
BpmI 1315 CTCCAG CTGGAG(16/14)
Bpu1102I 1147 GCTCAGC GC^T[ACGT]AGC
BsaBI 895 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 63 GACGTC G[GA]^CG[CT]C
BsaWI 638 TCCGGA [AT]^CCGG[AT]
BseAI 638 TCCGGA T^CCGGA
BsmBI 65 CGTCTC CGTCTC(1/5)
BspEI 638 TCCGGA T^CCGGA
BsrDI 1120 CATTGC GCAATG(2/0)
BssSI 411 CACGAG CACGAG(-5/-1)
BstBI 1207 TTCGAA TT^CGAA
Bsu36I 354 CCTGAGG CC^T[ACGT]AGG
CelII 1147 GCTCAGC GC^T[ACGT]AGC
CfoI 293 GCGC GCG^C
DraII 658 AGGTCCC [GA]G^G[ACGT]CC[CT]
Eco81I 354 CCTGAGG CC^T[ACGT]AGG
EcoO109I 658 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 896 ATGCAT ATGCA^T
FauI 951 GCGGG CCCGC(4/6)
HapII 639 CCGG C^CGG
HgaI 1584 GCGTC GACGC(5/10)
HhaI 293 GCGC GCG^C
Hin1I 63 GACGTC G[GA]^CG[CT]C
HinP1I 293 GCGC G^CGC
HindIII 1741 AAGCTT A^AGCTT
HpaII 639 CCGG C^CGG
MaeI 325 CTAG C^TAG
MamI 895 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 419 ACGCGT A^CGCGT
MroI 638 TCCGGA T^CCGGA
MspA1I 1150 CAGCTG C[AC]G^C[GT]G
MspI 639 CCGG C^CGG
NdeI 153 CATATG CA^TATG
NsiI 896 ATGCAT ATGCA^T
NspV 1207 TTCGAA TT^CGAA
PpuMI 658 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 562 ATTAAT AT^TAAT
PstI 1720 CTGCAG CTGCA^G
PvuII 1150 CAGCTG CAG^CTG
SfuI 1207 TTCGAA TT^CGAA
SspI 937 AATATT AAT^ATT
XbaI 324 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 951 GCGG CCGC(-3/-1)
1707 GCGG
AfaI 1219 GTAC GT^AC
1427 GTAC
AspHI 577 GAGCTC G[AT]GC[AT]^C
1675 GAGCTC
BalI 857 TGGCCA TGG^CCA
1163 TGGCCA
BanII 577 GAGCTC G[GA]GC[CT]^C
1675 GAGCTC
BbvI 1662 GCAGC GCAGC(8/12)
1722 GCAGC
BmyI 577 GAGCTC G[AGT]GC[ACT]^C
1675 GAGCTC
BseRI 305 GAGGAG GAGGAG(10/8)
875 GAGGAG
BsiHKAI 577 GAGCTC G[AT]GC[AT]^C
1675 GAGCTC
BsmAI 796 GAGAC GTCTC(1/5)
66 GTCTC
BsmFI 1136 GGGAC GGGAC(10/14)
660 GTCCC
Bsp1286I 577 GAGCTC G[AGT]GC[ACT]^C
1675 GAGCTC
BstAPI 525 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1779 GCAAATGATGC
BstYI 87 AGATCT [GA]^GATC[CT]
404 AGATCC
Cac8I 530 GCTTGC GC[ACGT]^[ACGT]GC
1725 GCTCGC
Cfr13I 169 GGCCC G^G[ACGT]CC
659 GGTCC
DraI 459 TTTAAA TTT^AAA
960 TTTAAA
EaeI 857 TGGCCA [CT]^GGCC[GA]
1163 TGGCCA
EcoRI 671 GAATTC G^AATTC
1369 GAATTC
EcoT14I 38 CCTTGG C^C[AT][AT]GG
1160 CCTTGG
MaeII 64 ACGT A^CGT
479 ACGT
MflI 87 AGATCT [GA]^GATC[CT]
404 AGATCC
MluNI 857 TGGCCA TGG^CCA
1163 TGGCCA
MscI 857 TGGCCA TGG^CCA
1163 TGGCCA
RsaI 1219 GTAC GT^AC
1427 GTAC
SacI 577 GAGCTC GAGCT^C
1675 GAGCTC
Sau96I 169 GGCCC G^G[ACGT]CC
659 GGTCC
StyI 38 CCTTGG C^C[AT][AT]GG
1160 CCTTGG
TseI 1662 GCAGC G^C[AT]GC
1722 GCAGC
XcmI 676 CCAGTGCTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1166 CCATGCTGATGTTGG
XhoII 87 AGATCT [GA]^GATC[CT]
404 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AcsI AluI AlwI ApoI
AvaI BsaJI BsiYI BslI BsoBI
BsrI BssKI BstF5I BstNI BstUI
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV PaeR7I PleI
Sau3AI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoI