(Circular) (Six-base) MAPSORT of: pFN21AB4121.seq Check: 9073 from: 1 to: 6514 pFN21AB4121 6514 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2072 4324 278 Size: 53 83 186 936 536 2252 2468 Fragments arranged by size: 2468 2252 936 536 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 3857 1937 Size: 1095 825 4594 Fragments arranged by size: 4594 1095 825 Acc65I G'GTAC_C Cuts at: 3835 3835 Size: 6514 AclI AA'CG_TT Cuts at: 1563 4641 5014 1563 Size: 3078 373 3063 Fragments arranged by size: 3078 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3084 4577 5961 6229 1632 Size: 1452 1493 1384 268 1917 Fragments arranged by size: 1917 1493 1452 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6317 AflIII A'CryG_T Cuts at: 1203 2424 5413 6390 1203 Size: 1221 2989 977 1327 Fragments arranged by size: 2989 1327 1221 977 AgeI A'CCGG_T Cuts at: 5345 5345 Size: 6514 AhdI GACnn_n'nnGTC Cuts at: 5243 5243 Size: 6514 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6514 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6514 AlwNI CAG_nnn'CTG Cuts at: 4257 5829 4257 Size: 1572 4942 ApaLI G'TGCA_C Cuts at: 4571 5727 4571 Size: 1156 5358 ApoI r'AATT_y Cuts at: 1493 2317 2676 2814 3254 3374 3527 3823 4049 Size: 824 359 138 440 120 153 296 226 Cuts at: 4049 4201 6310 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 824 440 359 296 226 153 152 138 120 AseI AT'TA_AT Cuts at: 160 1032 2568 5068 160 Size: 872 1536 2500 1606 Fragments arranged by size: 2500 1606 1536 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2275 3459 3839 1870 Size: 73 130 202 1184 380 4545 Fragments arranged by size: 4545 1184 380 202 130 73 BamHI G'GATC_C Cuts at: 3844 4211 3844 Size: 367 6147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3835 5290 618 Size: 325 217 627 2048 1455 1842 Fragments arranged by size: 2048 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2586 3684 3833 1391 Size: 1195 1098 149 4072 Fragments arranged by size: 4072 1195 1098 149 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4705 4739 1903 Size: 34 2768 34 3678 Fragments arranged by size: 3678 2768 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4406 5616 4406 Size: 1210 5304 BclI T'GATC_A Cuts at: 1548 1548 Size: 6514 BfrBI ATG'CAT Cuts at: 2903 2903 Size: 6514 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2870 5125 136 Size: 107 122 71 631 605 1198 2255 1525 Fragments arranged by size: 2255 1525 1198 631 605 122 107 71 BglII A'GATC_T Cuts at: 2092 6509 2092 Size: 4417 2097 BlpI GC'TnA_GC Cuts at: 3153 3931 3153 Size: 778 5736 Bme1580I G_kGCm'C Cuts at: 1790 4575 5731 1790 Size: 2785 1156 2573 Fragments arranged by size: 2785 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6514 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4245 5203 442 Size: 416 53 83 399 24 2828 958 1753 Fragments arranged by size: 2828 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6514 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2088 2088 Size: 6514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3305 5174 1239 Size: 2066 1869 2579 Fragments arranged by size: 2579 2066 1869 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3737 4639 5504 5802 3737 Size: 902 865 298 4449 Fragments arranged by size: 4449 902 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5177 882 Size: 617 3678 2219 Fragments arranged by size: 3678 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4305 493 Size: 711 3101 2702 Fragments arranged by size: 3101 2702 711 BsaBI GATnn'nnATC Cuts at: 2904 6508 2904 Size: 3604 2910 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 4321 4703 5338 Size: 53 83 186 936 536 2252 382 635 Cuts at: 5338 275 Size: 1451 Fragments arranged by size: 2252 1451 936 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2643 4947 5345 5619 5766 6177 2643 Size: 2304 398 274 147 411 2980 Fragments arranged by size: 2980 2304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 1178 Size: 30 165 30 636 30 5623 Fragments arranged by size: 5623 636 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2325 2895 1186 Size: 1139 570 4805 Fragments arranged by size: 4805 1139 570 BseYI C'CCAG_C Cuts at: 2846 5717 6213 2846 Size: 2871 496 3147 Fragments arranged by size: 3147 2871 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5941 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3964 4725 5753 1540 Size: 275 186 1963 761 1028 2301 Fragments arranged by size: 2301 1963 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2586 3684 4575 4660 5731 2586 Size: 1098 891 85 1071 3369 Fragments arranged by size: 3369 1098 1071 891 85 BsmI GAATG_Cn' Cuts at: 4025 4118 4025 Size: 93 6421 BsmBI CGTCTCn'nnnn_ Cuts at: 2076 2076 Size: 6514 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2586 3684 3833 4575 4660 5731 1391 Size: 399 796 1098 149 742 85 1071 2174 Fragments arranged by size: 2174 1098 1071 796 742 399 149 85 BspEI T'CCGG_A Cuts at: 2643 6177 2643 Size: 3534 2980 BspHI T'CATG_A Cuts at: 4403 6133 4403 Size: 1730 4784 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3869 844 Size: 3025 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4401 1394 Size: 429 2578 3507 Fragments arranged by size: 3507 2578 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3124 5009 5183 65 Size: 1183 1876 1885 174 1396 Fragments arranged by size: 1885 1876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5158 5345 1700 Size: 110 3348 187 2869 Fragments arranged by size: 3348 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6514 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6514 BssSI C'ACGA_G Cuts at: 2416 4574 5586 2416 Size: 2158 1012 3344 Fragments arranged by size: 3344 2158 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 3790 794 Size: 1742 1254 3518 Fragments arranged by size: 3518 1742 1254 BstBI TT'CG_AA Cuts at: 3213 5318 3213 Size: 2105 4409 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6514 BstYI r'GATC_y Cuts at: 1972 2092 2409 3844 4211 4596 4613 6065 6151 Size: 120 317 1435 367 385 17 1452 86 Cuts at: 6151 6163 6509 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1435 385 367 346 317 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2360 2360 Size: 6514 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5023 Fragments arranged by size: 5023 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5775 Fragments arranged by size: 5775 683 56 BtsI GCAGTG_nn' Cuts at: 2865 3136 4030 4824 4844 2865 Size: 271 894 794 20 4535 Fragments arranged by size: 4535 894 794 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6479 DraI TTT'AAA Cuts at: 2466 2967 3818 2466 Size: 501 851 5162 Fragments arranged by size: 5162 851 501 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5521 809 Size: 507 4205 1802 Fragments arranged by size: 4205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2862 3168 3961 Size: 54 1425 50 275 18 1032 306 793 Cuts at: 3961 4850 6479 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 793 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3961 1537 Size: 275 2149 4090 Fragments arranged by size: 4090 2149 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2389 3101 4444 1353 Size: 829 207 712 1343 3423 Fragments arranged by size: 3423 1343 829 712 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5101 5475 5621 387 Size: 1064 138 3512 374 146 1280 Fragments arranged by size: 3512 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2584 3682 2584 Size: 1098 5416 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3084 3305 4577 5174 5961 6229 1239 Size: 393 1452 221 1272 597 787 268 1524 Fragments arranged by size: 1524 1452 1272 787 597 393 268 221 EcoO109I rG'GnC_Cy Cuts at: 2664 2664 Size: 6514 EcoRI G'AATT_C Cuts at: 2676 3374 3823 4201 2676 Size: 698 449 378 4989 Fragments arranged by size: 4989 698 449 378 FspI TGC'GCA Cuts at: 5020 5020 Size: 6514 HaeII r_GCGC'y Cuts at: 1678 5661 1678 Size: 3983 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2067 2071 2099 2181 Size: 32 135 32 467 28 4 28 82 Cuts at: 2181 2213 5155 5187 5229 5261 1373 Size: 32 2942 32 42 32 2626 Fragments arranged by size: 2942 2626 467 135 82 42 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 3858 4104 669 Size: 988 281 1920 246 3079 Fragments arranged by size: 3079 1920 988 281 246 HindIII A'AGCT_T Cuts at: 748 3746 748 Size: 2998 3516 HpaI GTT'AAC Cuts at: 4104 4104 Size: 6514 KpnI G_GTAC'C Cuts at: 3839 3839 Size: 6514 MfeI C'AATT_G Cuts at: 4113 4113 Size: 6514 MluI A'CGCG_T Cuts at: 2424 6390 2424 Size: 3966 2548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3162 5628 5812 1305 Size: 1857 2466 184 2007 Fragments arranged by size: 2466 2007 1857 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2864 3170 10 Size: 54 1425 343 1032 306 3354 Fragments arranged by size: 3354 1425 1032 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 3129 3176 3294 4472 4831 4990 518 Size: 648 1963 47 118 1178 359 159 2042 Fragments arranged by size: 2042 1963 1178 648 359 159 118 47 MspA1I CmG'CkG Cuts at: 1606 3157 3930 4607 5755 6000 1606 Size: 1551 773 677 1148 245 2120 Fragments arranged by size: 2120 1551 1148 773 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5023 Fragments arranged by size: 5023 939 552 NdeI CA'TA_TG Cuts at: 387 2159 387 Size: 1772 4742 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6514 NotI GC'GGCC_GC Cuts at: 3961 3961 Size: 6514 NsiI A_TGCA'T Cuts at: 2905 2905 Size: 6514 NspI r_CATG'y Cuts at: 1132 1229 3872 5417 1132 Size: 97 2643 1545 2229 Fragments arranged by size: 2643 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6514 PciI A'CATG_T Cuts at: 5413 5413 Size: 6514 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6514 PfoI T'CCnGG_A Cuts at: 2119 2119 Size: 6514 PmeI GTTT'AAAC Cuts at: 3818 3818 Size: 6514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3714 3746 4583 4615 6096 6128 3714 Size: 32 837 32 1481 32 4100 Fragments arranged by size: 4100 1481 837 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2664 2664 Size: 6514 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6514 PsiI TTA'TAA Cuts at: 3398 4084 3398 Size: 686 5828 PstI C_TGCA'G Cuts at: 830 3729 3866 830 Size: 2899 137 3478 Fragments arranged by size: 3478 2899 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6514 PvuII CAG'CTG Cuts at: 3157 3157 Size: 6514 SacI G_AGCT'C Cuts at: 2586 3684 2586 Size: 1098 5416 SalI G'TCGA_C Cuts at: 1936 3856 1936 Size: 1920 4594 SbfI CC_TGCA'GG Cuts at: 3866 3866 Size: 6514 ScaI AGT'ACT Cuts at: 4762 6474 4762 Size: 1712 4802 SfcI C'TryA_G Cuts at: 826 1045 3193 3363 3725 3862 4997 5678 5869 Size: 219 2148 170 362 137 1135 681 191 Cuts at: 5869 826 Size: 1471 Fragments arranged by size: 2148 1471 1135 681 362 219 191 170 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6514 SmaI CCC'GGG Cuts at: 1872 3841 1872 Size: 1969 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 2275 3459 3716 4618 5519 Size: 197 926 130 202 1184 257 902 901 Cuts at: 5519 5781 820 Size: 262 1553 Fragments arranged by size: 1553 1184 926 902 901 262 257 202 197 130 SnaBI TAC'GTA Cuts at: 493 4305 493 Size: 3812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6514 SphI G_CATG'C Cuts at: 1132 3872 1132 Size: 2740 3774 SspI AAT'ATT Cuts at: 5 52 2944 4438 5 Size: 47 2892 1494 2081 Fragments arranged by size: 2892 2081 1494 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6514 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 3165 3953 513 Size: 552 939 39 1122 788 3074 Fragments arranged by size: 3074 1122 939 788 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2255 3595 4541 4558 4711 4896 5341 Size: 1255 675 1340 946 17 153 185 445 Cuts at: 5341 325 Size: 1498 Fragments arranged by size: 1498 1340 1255 946 675 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4760 6472 96 Size: 274 80 33 51 1443 2783 1712 138 Fragments arranged by size: 2783 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6474 6506 6474 Size: 32 6482 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6514 XbaI T'CTAG_A Cuts at: 2329 3850 2329 Size: 1521 4993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2688 3178 2688 Size: 490 6024 XhoI C'TCGA_G Cuts at: 1943 2073 2275 3459 1943 Size: 130 202 1184 4998 Fragments arranged by size: 4998 1184 202 130 XmaI C'CCGG_G Cuts at: 1870 3839 1870 Size: 1969 4545 XmnI GAAnn'nnTTC Cuts at: 4643 4643 Size: 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2070 4322 276 Size: 53 83 186 936 536 2252 2468 Fragments arranged by size: 2468 2252 936 536 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI