(Circular) (Six-base) MAPSORT of: pFN21AB4102.seq Check: 8689 from: 1 to: 6850 pFN21AB4102 6850 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3684 3684 Size: 6850 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4660 278 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4193 1937 Size: 2256 4594 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 4977 5350 1563 Size: 3414 373 3063 Fragments arranged by size: 3414 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2316 3282 4913 6297 6565 1632 Size: 684 966 1631 1384 268 1917 Fragments arranged by size: 1917 1631 1384 966 684 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6653 AflIII A'CryG_T Cuts at: 1203 2022 3843 5749 6726 1203 Size: 819 1821 1906 977 1327 Fragments arranged by size: 1906 1821 1327 977 819 AgeI A'CCGG_T Cuts at: 5681 5681 Size: 6850 AhdI GACnn_n'nnGTC Cuts at: 5579 5579 Size: 6850 AleI CACnn'nnGTG Cuts at: 1166 2744 3416 4078 1166 Size: 1578 672 662 3938 Fragments arranged by size: 3938 1578 672 662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3107 1184 Size: 1923 4927 AlwNI CAG_nnn'CTG Cuts at: 2179 2290 2786 2834 2894 3127 3260 3686 4593 Size: 111 496 48 60 233 133 426 907 Cuts at: 4593 6165 2179 Size: 1572 2864 Fragments arranged by size: 2864 1572 907 496 426 233 133 111 60 48 ApaLI G'TGCA_C Cuts at: 4907 6063 4907 Size: 1156 5694 ApoI r'AATT_y Cuts at: 1493 4121 4159 4385 4537 6646 1493 Size: 2628 38 226 152 2109 1697 Fragments arranged by size: 2628 2109 1697 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 5404 160 Size: 872 4372 1606 Fragments arranged by size: 4372 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 4175 1870 Size: 73 2232 4545 Fragments arranged by size: 4545 2232 73 BamHI G'GATC_C Cuts at: 4180 4547 4180 Size: 367 6483 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2732 3019 3377 4171 5626 Size: 325 217 627 945 287 358 794 1455 Cuts at: 5626 618 Size: 1842 Fragments arranged by size: 1842 1455 945 794 627 358 325 287 217 BanII G_rGCy'C Cuts at: 1391 2043 4169 1391 Size: 652 2126 4072 Fragments arranged by size: 4072 2126 652 BbeI G_GCGC'C Cuts at: 3381 3381 Size: 6850 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3508 928 Size: 971 1609 4270 Fragments arranged by size: 4270 1609 971 BbvCI CC'TCA_GC Cuts at: 2827 2895 3080 2827 Size: 68 185 6597 Fragments arranged by size: 6597 185 68 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3436 3470 3553 3587 4095 4129 5041 Size: 34 1499 34 83 34 508 34 912 Cuts at: 5041 5075 1903 Size: 34 3678 Fragments arranged by size: 3678 1499 912 508 83 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4742 5952 4742 Size: 1210 5640 BclI T'GATC_A Cuts at: 1548 1548 Size: 6850 BfrBI ATG'CAT Cuts at: 3976 3976 Size: 6850 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 2175 2219 Size: 107 122 71 631 605 448 55 44 Cuts at: 2219 2567 3100 3317 5461 136 Size: 348 533 217 2144 1525 Fragments arranged by size: 2144 1525 631 605 533 448 348 217 122 107 71 55 44 BglII A'GATC_T Cuts at: 3735 6845 3735 Size: 3110 3740 BlpI GC'TnA_GC Cuts at: 4267 4267 Size: 6850 Bme1580I G_kGCm'C Cuts at: 1790 2724 2910 3190 3540 3566 4911 6067 1790 Size: 934 186 280 350 26 1345 1156 2573 Fragments arranged by size: 2573 1345 1156 934 350 280 186 26 BmgBI CAC'GTC Cuts at: 1318 3844 1318 Size: 2526 4324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2445 3571 4072 Size: 416 53 83 399 24 1028 1126 501 Cuts at: 4072 4581 5539 442 Size: 509 958 1753 Fragments arranged by size: 1753 1126 1028 958 509 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3982 4087 3982 Size: 105 6745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2201 2267 2323 2656 2677 3149 5510 Size: 941 21 66 56 333 21 472 2361 Cuts at: 5510 1239 Size: 2579 Fragments arranged by size: 2579 2361 941 472 333 66 56 21 21 Bpu10I CC'TnA_GC Cuts at: 2827 2895 2960 3080 3713 2827 Size: 68 65 120 633 5964 Fragments arranged by size: 5964 633 120 68 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3350 3812 4975 5840 6138 3350 Size: 462 1163 865 298 4062 Fragments arranged by size: 4062 1163 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2063 2303 2544 4061 5513 882 Size: 617 564 240 241 1517 1452 2219 Fragments arranged by size: 2219 1517 1452 617 564 241 240 BsaAI yAC'GTr Cuts at: 493 1204 2146 4641 493 Size: 711 942 2495 2702 Fragments arranged by size: 2702 2495 942 711 BsaBI GATnn'nnATC Cuts at: 6844 6844 Size: 6850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3168 3378 4657 5039 Size: 53 83 186 936 1635 210 1279 382 Cuts at: 5039 5674 275 Size: 635 1451 Fragments arranged by size: 1635 1451 1279 936 635 382 210 186 83 53 BsaWI w'CCGG_w Cuts at: 2301 5283 5681 5955 6102 6513 2301 Size: 2982 398 274 147 411 2638 Fragments arranged by size: 2982 2638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3519 3549 1178 Size: 30 165 30 2116 30 4479 Fragments arranged by size: 4479 2116 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2620 2894 3044 3242 3353 4076 4079 1186 Size: 1434 274 150 198 111 723 3 3957 Fragments arranged by size: 3957 1434 723 274 198 150 111 3 BseYI C'CCAG_C Cuts at: 2423 3171 4132 4145 6053 6549 2423 Size: 748 961 13 1908 496 2724 Fragments arranged by size: 2724 1908 961 748 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3406 3656 1163 Size: 573 1670 250 4357 Fragments arranged by size: 4357 1670 573 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4300 5061 6089 1540 Size: 275 186 2299 761 1028 2301 Fragments arranged by size: 2301 2299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3558 4911 4996 6067 3558 Size: 1353 85 1071 4341 Fragments arranged by size: 4341 1353 1071 85 BsmI GAATG_Cn' Cuts at: 4361 4454 4361 Size: 93 6757 BsmBI CGTCTCn'nnnn_ Cuts at: 2129 2129 Size: 6850 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2724 2910 3190 3540 3558 3566 Size: 399 253 681 186 280 350 18 8 Cuts at: 3566 4169 4911 4996 6067 1391 Size: 603 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 603 399 350 280 253 186 85 18 8 BspEI T'CCGG_A Cuts at: 6513 6513 Size: 6850 BspHI T'CATG_A Cuts at: 4739 6469 4739 Size: 1730 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 3037 3094 3660 3678 3684 4011 4038 Size: 1325 868 57 566 18 6 327 27 Cuts at: 4038 4205 844 Size: 167 3489 Fragments arranged by size: 3489 1325 868 566 327 167 57 27 18 6 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2434 4737 1394 Size: 429 208 403 2303 3507 Fragments arranged by size: 3507 2303 429 403 208 BsrDI GCAATG_nn' Cuts at: 65 1248 5345 5519 65 Size: 1183 4097 174 1396 Fragments arranged by size: 4097 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3022 4017 5494 5681 1700 Size: 110 1212 995 1477 187 2869 Fragments arranged by size: 2869 1477 1212 995 187 110 BsrGI T'GTAC_A Cuts at: 96 2224 96 Size: 2128 4722 BssHII G'CGCG_C Cuts at: 1925 2013 2097 2123 1925 Size: 88 84 26 6652 Fragments arranged by size: 6652 88 84 26 BssSI C'ACGA_G Cuts at: 4910 5922 4910 Size: 1012 5838 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6850 BstBI TT'CG_AA Cuts at: 5654 5654 Size: 6850 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6850 BstXI CCAn_nnnn'nTGG Cuts at: 2050 2050 Size: 6850 BstYI r'GATC_y Cuts at: 1972 2490 3735 4180 4547 4932 4949 6401 6487 Size: 518 1245 445 367 385 17 1452 86 Cuts at: 6487 6499 6845 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1245 518 445 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3097 3097 Size: 6850 BtgI C'CryG_G Cuts at: 513 1065 2061 3510 3522 513 Size: 552 996 1449 12 3841 Fragments arranged by size: 3841 1449 996 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3907 487 Size: 683 56 2681 3430 Fragments arranged by size: 3430 2681 683 56 BtsI GCAGTG_nn' Cuts at: 2134 3347 3540 4366 5160 5180 2134 Size: 1213 193 826 794 20 3804 Fragments arranged by size: 3804 1213 826 794 193 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6815 DraI TTT'AAA Cuts at: 4154 4154 Size: 6850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3695 5857 809 Size: 507 2379 2162 1802 Fragments arranged by size: 2379 2162 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2584 2982 Size: 54 1425 50 275 18 282 472 398 Cuts at: 2982 3024 3507 3858 4297 5186 6815 8 Size: 42 483 351 439 889 1629 43 Fragments arranged by size: 1629 1425 889 483 472 439 398 351 282 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 4297 1537 Size: 275 2485 4090 Fragments arranged by size: 4090 2485 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4780 1353 Size: 3427 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 5437 5811 5957 387 Size: 1064 138 462 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 462 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2201 2267 2316 2323 2656 2677 Size: 393 548 21 66 49 7 333 21 Cuts at: 2677 3149 3282 4913 5510 6297 6565 1239 Size: 472 133 1631 597 787 268 1524 Fragments arranged by size: 1631 1524 787 597 548 472 393 333 268 133 66 49 21 21 7 EcoNI CCTnn'n_nnAGG Cuts at: 2183 4058 2183 Size: 1875 4975 EcoO109I rG'GnC_Cy Cuts at: 2176 2327 2560 3049 3076 2176 Size: 151 233 489 27 5950 Fragments arranged by size: 5950 489 233 151 27 EcoRI G'AATT_C Cuts at: 4121 4159 4537 4121 Size: 38 378 6434 Fragments arranged by size: 6434 378 38 FspI TGC'GCA Cuts at: 3788 3953 5356 3788 Size: 165 1403 5282 Fragments arranged by size: 5282 1403 165 FspAI rTGC'GCAy Cuts at: 3788 3788 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 2548 3381 5997 1678 Size: 870 833 2616 2531 Fragments arranged by size: 2616 2531 870 833 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 3284 3316 5491 Size: 32 135 32 728 32 952 32 2175 Cuts at: 5491 5523 5565 5597 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2175 952 728 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4194 4440 669 Size: 988 281 2256 246 3079 Fragments arranged by size: 3079 2256 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KasI G'GCGC_C Cuts at: 3377 3377 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 4449 4449 Size: 6850 MluI A'CGCG_T Cuts at: 2022 6726 2022 Size: 4704 2146 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5964 6148 1305 Size: 4659 184 2007 Fragments arranged by size: 4659 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2586 2984 3509 10 Size: 54 1425 343 754 398 525 3351 Fragments arranged by size: 3351 1425 754 525 398 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2382 2744 2996 3416 3726 3887 Size: 648 1071 145 362 252 420 310 161 Cuts at: 3887 3911 4078 4808 5167 5326 518 Size: 24 167 730 359 159 2042 Fragments arranged by size: 2042 1071 730 648 420 362 359 310 252 167 161 159 145 24 MspA1I CmG'CkG Cuts at: 1606 2063 2625 2697 2894 3216 3631 3683 3866 Size: 457 562 72 197 322 415 52 183 Cuts at: 3866 4266 4943 6091 6336 1606 Size: 400 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 562 457 415 400 322 245 197 183 72 52 NaeI GCC'GGC Cuts at: 3024 3024 Size: 6850 NarI GG'CG_CC Cuts at: 3378 3378 Size: 6850 NcoI C'CATG_G Cuts at: 513 1065 3510 3522 513 Size: 552 2445 12 3841 Fragments arranged by size: 3841 2445 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6850 NgoMIV G'CCGG_C Cuts at: 3022 3022 Size: 6850 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6850 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NsiI A_TGCA'T Cuts at: 3978 3978 Size: 6850 NspI r_CATG'y Cuts at: 1132 1229 3457 3980 4208 5753 1132 Size: 97 2228 523 228 1545 2229 Fragments arranged by size: 2229 2228 1545 523 228 97 PasI CC'CwG_GG Cuts at: 1348 2851 1348 Size: 1503 5347 PciI A'CATG_T Cuts at: 5749 5749 Size: 6850 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 1108 Size: 1272 5578 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4919 4951 6432 6464 4919 Size: 32 1481 32 5305 Fragments arranged by size: 5305 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3049 3076 3049 Size: 27 6823 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6850 PsiI TTA'TAA Cuts at: 4420 4420 Size: 6850 PstI C_TGCA'G Cuts at: 830 2175 2395 2800 3840 4008 4035 4202 830 Size: 1345 220 405 1040 168 27 167 3478 Fragments arranged by size: 3478 1345 1040 405 220 168 167 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 2697 2894 3631 3683 3866 2697 Size: 197 737 52 183 5681 Fragments arranged by size: 5681 737 197 183 52 SacII CC_GC'GG Cuts at: 2064 2064 Size: 6850 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SbfI CC_TGCA'GG Cuts at: 2175 3840 4035 4202 2175 Size: 1665 195 167 4823 Fragments arranged by size: 4823 1665 195 167 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SfcI C'TryA_G Cuts at: 826 1045 2171 2391 2796 3435 3836 3981 4004 Size: 219 1126 220 405 639 401 145 23 Cuts at: 4004 4031 4198 5333 6014 6205 826 Size: 27 167 1135 681 191 1471 Fragments arranged by size: 1471 1135 1126 681 639 405 401 220 219 191 167 145 27 23 SfiI GGCCn_nnn'nGGCC Cuts at: 2567 3317 2567 Size: 750 6100 SfoI GGC'GCC Cuts at: 3379 3379 Size: 6850 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 4177 1872 Size: 2305 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3365 3827 4954 5855 6117 820 Size: 197 926 1422 462 1127 901 262 1553 Fragments arranged by size: 1553 1422 1127 926 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 3980 4208 1132 Size: 2848 228 3774 Fragments arranged by size: 3774 2848 228 SspI AAT'ATT Cuts at: 5 52 4774 5 Size: 47 4722 2081 Fragments arranged by size: 4722 2081 47 StuI AGG'CCT Cuts at: 1517 2481 3014 1517 Size: 964 533 5353 Fragments arranged by size: 5353 964 533 StyI C'CwwG_G Cuts at: 513 1065 2772 3312 3510 3522 3612 4289 513 Size: 552 1707 540 198 12 90 677 3074 Fragments arranged by size: 3074 1707 677 552 540 198 90 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2533 2719 2750 4877 4894 5047 5232 Size: 1255 953 186 31 2127 17 153 185 Cuts at: 5232 5677 325 Size: 445 1498 Fragments arranged by size: 2127 1498 1255 953 445 186 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2224 2256 5096 Size: 274 80 33 51 1443 247 32 2840 Cuts at: 5096 6808 96 Size: 1712 138 Fragments arranged by size: 2840 1712 1443 274 247 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3519 3551 3807 3839 6810 6842 3519 Size: 32 256 32 2971 32 3527 Fragments arranged by size: 3527 2971 256 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2314 1316 Size: 998 5852 XbaI T'CTAG_A Cuts at: 4186 4186 Size: 6850 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3388 3388 Size: 6850 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6850 XmaI C'CCGG_G Cuts at: 1870 4175 1870 Size: 2305 4545 XmnI GAAnn'nnTTC Cuts at: 4979 4979 Size: 6850 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4658 276 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI