Restriction map for
pFN21AB4066
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccII AcyI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AspHI
AspI AvaI AvrII BalI BbeI
BbsI BbvCI BcgI BciVI BclI
BlnI BpuAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1120 GGTACC G^GTACC
AccIII 300 TCCGGA T^CCGGA
AclI 43 AACGTT AA^CGTT
AflII 948 CTTAAG C^TTAAG
AlwNI 157 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1162 ATTAAT AT^TAAT
AsnI 1162 ATTAAT AT^TAAT
Asp718I 1120 GGTACC G^GTACC
AviII 834 TGCGCA TGC^GCA
BamHI 657 GGATCC G^GATCC
BanI 1120 GGTACC G^G[CT][GA]CC
BbrPI 60 CACGTG CAC^GTG
BfrI 948 CTTAAG C^TTAAG
BglI 635 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 900 AGATCT A^GATCT
BlpI 753 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 753 GCTAAGC GC^T[ACGT]AGC
BsaAI 60 CACGTG [CT]AC^GT[GA]
BsaWI 300 TCCGGA [AT]^CCGG[AT]
BseAI 300 TCCGGA T^CCGGA
BseRI 130 GAGGAG GAGGAG(10/8)
BsgI 439 CTGCAC GTGCAG(16/14)
BsmFI 290 GGGAC GGGAC(10/14)
BspEI 300 TCCGGA T^CCGGA
BsrDI 1185 CATTGC GCAATG(2/0)
CelII 753 GCTAAGC GC^T[ACGT]AGC
CfoI 835 GCGC GCG^C
EarI 210 GAAGAG CTCTTC(1/4)
FspI 834 TGCGCA TGC^GCA
HgaI 391 GCGTC GACGC(5/10)
HhaI 835 GCGC GCG^C
HinP1I 835 GCGC G^CGC
KpnI 1120 GGTACC GGTAC^C
Ksp632I 210 GAAGAG CTCTTC(1/4)
MroI 300 TCCGGA T^CCGGA
MslI 442 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 60 CACGTG CAC^GTG
PmlI 60 CACGTG CAC^GTG
PshBI 1162 ATTAAT AT^TAAT
Psp1406I 43 AACGTT AA^CGTT
PstI 742 CTGCAG CTGCA^G
SapI 210 GAAGAGC GCTCTTC(1/4)
XbaI 942 TCTAGA T^CTAGA
XcmI 277 CCAGGTGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwI 658 GATCC GGATC(4/5)
657 GGATC
Asp700I 320 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1141 GAAGCCCTTC
AvaII 510 GGACC G^G[AT]CC
558 GGACC
BanII 133 GAGCCC G[GA]GC[CT]^C
189 GAGCCC
BcnI 183 CCCGG CC^[GC]GG
444 CCCGG
BfaI 388 CTAG C^TAG
943 CTAG
BmyI 133 GAGCCC G[AGT]GC[ACT]^C
189 GAGCCC
Bsp1286I 133 GAGCCC G[AGT]GC[ACT]^C
189 GAGCCC
Bsp1407I 546 TGTACA T^GTACA
819 TGTACA
BsrGI 546 TGTACA T^GTACA
819 TGTACA
BstAPI 744 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1232 GCATCAAATGC
BstYI 657 GGATCC [GA]^GATC[CT]
900 AGATCT
Cac8I 248 GCAGGC GC[ACGT]^[ACGT]GC
644 GCCTGC
DsaI 196 CCACGG C^C[GA][CT]GG
594 CCACGG
MaeI 388 CTAG C^TAG
943 CTAG
MaeII 44 ACGT A^CGT
61 ACGT
MaeIII 224 GTGAC ^GT[ACGT]AC
451 GTGAC
MboII 210 GAAGA GAAGA(8/7)
920 GAAGA
MflI 657 GGATCC [GA]^GATC[CT]
900 AGATCT
NciI 183 CCCGG CC^[GC]GG
444 CCCGG
NspI 816 GCATGT [GA]CATG^[CT]
837 GCATGT
PflMI 513 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1195 CCAACTATTGG
PleI 292 GACTC GAGTC(4/5)
400 GAGTC
PvuII 759 CAGCTG CAG^CTG
859 CAGCTG
SfaNI 923 GATGC GCATC(5/9)
1232 GCATC
SmlI 470 CTCAAG C^T[CT][GA]AG
948 CTTAAG
SspBI 546 TGTACA T^GTACA
819 TGTACA
StuI 250 AGGCCT AGG^CCT
642 AGGCCT
TaqI 264 TCGA T^CGA
565 TCGA
Tsp45I 224 GTGAC ^GT[GC]AC
451 GTGAC
TthHB8I 264 TCGA T^CGA
565 TCGA
Van91I 513 CCAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1195 CCAACTATTGG
XhoII 657 GGATCC [GA]^GATC[CT]
900 AGATCT
XmnI 320 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1141 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MboI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp509I TspRI