Restriction map for
pFN21AB4066
(Linear) (Six-base) MAPSORT of: KIBB4066.seq Check: 2855 from: 1 to: 1272
KIBB4066.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
Acc65I G'GTAC_C
Cuts at: 0 1120 1272
Size: 1120 152
AclI AA'CG_TT
Cuts at: 0 44 1272
Size: 44 1228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1272
Size: 229 1043
AflII C'TTAA_G
Cuts at: 0 948 1272
Size: 948 324
AlwNI CAG_nnn'CTG
Cuts at: 0 162 1272
Size: 162 1110
ApoI r'AATT_y
Cuts at: 0 690 733 988 1073 1272
Size: 690 43 255 85 199
Fragments arranged by size:
690 255 199 85 43
AseI AT'TA_AT
Cuts at: 0 1163 1272
Size: 1163 109
BamHI G'GATC_C
Cuts at: 0 657 1272
Size: 657 615
BanI G'GyrC_C
Cuts at: 0 1120 1272
Size: 1120 152
BanII G_rGCy'C
Cuts at: 0 137 193 1272
Size: 137 56 1079
Fragments arranged by size:
1079 137 56
BglI GCCn_nnn'nGGC
Cuts at: 0 641 1272
Size: 641 631
BglII A'GATC_T
Cuts at: 0 900 1272
Size: 900 372
BlpI GC'TnA_GC
Cuts at: 0 754 1272
Size: 754 518
BmrI ACTGGGnnnn_n'
Cuts at: 0 1256 1272
Size: 1256 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 142 874 1272
Size: 1 141 732 398
Fragments arranged by size:
732 398 141 1
Bpu10I CC'TnA_GC
Cuts at: 0 254 344 1272
Size: 254 90 928
Fragments arranged by size:
928 254 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1272
Size: 455 817
BsaI GGTCTCn'nnnn_
Cuts at: 0 117 363 841 1272
Size: 117 246 478 431
Fragments arranged by size:
478 431 246 117
BsaAI yAC'GTr
Cuts at: 0 62 1272
Size: 62 1210
BsaWI w'CCGG_w
Cuts at: 0 300 1272
Size: 300 972
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 1272
Size: 145 1127
BseYI C'CCAG_C
Cuts at: 0 857 1272
Size: 857 415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1272
Size: 424 848
Bsp1286I G_dGCh'C
Cuts at: 0 137 193 1272
Size: 137 56 1079
Fragments arranged by size:
1079 137 56
BspEI T'CCGG_A
Cuts at: 0 300 1272
Size: 300 972
BsrDI GCAATG_nn'
Cuts at: 0 1184 1272
Size: 1184 88
BsrGI T'GTAC_A
Cuts at: 0 546 819 1272
Size: 546 273 453
Fragments arranged by size:
546 453 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 750 1238 1272
Size: 750 488 34
Fragments arranged by size:
750 488 34
BstYI r'GATC_y
Cuts at: 0 657 900 1272
Size: 657 243 372
Fragments arranged by size:
657 372 243
BtgI C'CryG_G
Cuts at: 0 196 594 1272
Size: 196 398 678
Fragments arranged by size:
678 398 196
BtsI GCAGTG_nn'
Cuts at: 0 1249 1272
Size: 1249 23
EarI CTCTTCn'nnn_
Cuts at: 0 205 1272
Size: 205 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 1272
Size: 105 1167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 142 229 874 1272
Size: 1 141 87 645 398
Fragments arranged by size:
645 398 141 87 1
FspI TGC'GCA
Cuts at: 0 836 1272
Size: 836 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 1272
Size: 366 32 874
Fragments arranged by size:
874 366 32
KpnI G_GTAC'C
Cuts at: 0 1124 1272
Size: 1124 148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1272
Size: 1035 237
MslI CAynn'nnrTG
Cuts at: 0 446 1272
Size: 446 826
MspA1I CmG'CkG
Cuts at: 0 491 761 861 1272
Size: 491 270 100 411
Fragments arranged by size:
491 411 270 100
NspI r_CATG'y
Cuts at: 0 820 841 1272
Size: 820 21 431
Fragments arranged by size:
820 431 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 1201 1272
Size: 519 682 71
Fragments arranged by size:
682 519 71
PfoI T'CCnGG_A
Cuts at: 0 17 1272
Size: 17 1255
PmlI CAC'GTG
Cuts at: 0 62 1272
Size: 62 1210
PstI C_TGCA'G
Cuts at: 0 746 1272
Size: 746 526
PvuII CAG'CTG
Cuts at: 0 761 861 1272
Size: 761 100 411
Fragments arranged by size:
761 411 100
SapI GCTCTTCn'nnn_
Cuts at: 0 205 1272
Size: 205 1067
SfcI C'TryA_G
Cuts at: 0 742 876 1156 1272
Size: 742 134 280 116
Fragments arranged by size:
742 280 134 116
SmlI C'TyrA_G
Cuts at: 0 470 948 1272
Size: 470 478 324
Fragments arranged by size:
478 470 324
StuI AGG'CCT
Cuts at: 0 252 644 1272
Size: 252 392 628
Fragments arranged by size:
628 392 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 704 871 1272
Size: 704 167 401
Fragments arranged by size:
704 401 167
TatI w'GTAC_w
Cuts at: 0 546 819 1272
Size: 546 273 453
Fragments arranged by size:
546 453 273
XbaI T'CTAG_A
Cuts at: 0 942 1272
Size: 942 330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 284 1272
Size: 284 988
XmnI GAAnn'nnTTC
Cuts at: 0 324 1145 1272
Size: 324 821 127
Fragments arranged by size:
821 324 127
Enzymes that do cut:
Acc65I AclI AcuI AflII AlwNI ApoI AseI BamHI
BanI BanII BglI BglII BlpI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI Bsp1286I
BspEI BsrDI BsrGI BstAPI BstYI BtgI BtsI EarI
EciI Eco57MI FspI Hin4I KpnI MmeI MslI MspA1I
NspI PflMI PfoI PmlI PstI PvuII SapI SfcI
SmlI StuI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
Bme1580I BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TstI Tth111I XhoI XmaI ZraI