(Circular) (Six-base) MAPSORT of: pFN21AB3965.seq Check: 6202 from: 1 to: 6997 pFN21AB3965 6997 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2132 2132 Size: 6997 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2501 4807 278 Size: 53 83 186 936 965 2306 2468 Fragments arranged by size: 2468 2306 965 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 3423 3533 4340 1937 Size: 739 747 110 807 4594 Fragments arranged by size: 4594 807 747 739 110 Acc65I G'GTAC_C Cuts at: 4318 4318 Size: 6997 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 2195 2559 2570 2771 5060 6444 6712 Size: 466 97 364 11 201 2289 1384 268 Cuts at: 6712 1632 Size: 1917 Fragments arranged by size: 2289 1917 1384 466 364 268 201 97 11 AfeI AGC'GCT Cuts at: 3560 3560 Size: 6997 AflII C'TTAA_G Cuts at: 820 1017 2783 820 Size: 197 1766 5034 Fragments arranged by size: 5034 1766 197 AflIII A'CryG_T Cuts at: 1203 2700 5896 6873 1203 Size: 1497 3196 977 1327 Fragments arranged by size: 3196 1497 1327 977 AgeI A'CCGG_T Cuts at: 5828 5828 Size: 6997 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 2853 3618 1166 Size: 1687 765 4545 Fragments arranged by size: 4545 1687 765 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3403 3636 1184 Size: 2219 233 4545 Fragments arranged by size: 4545 2219 233 AlwNI CAG_nnn'CTG Cuts at: 2173 2278 3479 3708 4740 6312 2173 Size: 105 1201 229 1032 1572 2858 Fragments arranged by size: 2858 1572 1201 1032 229 105 ApaLI G'TGCA_C Cuts at: 5054 6210 5054 Size: 1156 5841 ApoI r'AATT_y Cuts at: 1493 2325 4306 4532 4684 6793 1493 Size: 832 1981 226 152 2109 1697 Fragments arranged by size: 2109 1981 1697 832 226 152 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 4322 1870 Size: 73 2379 4545 Fragments arranged by size: 4545 2379 73 BamHI G'GATC_C Cuts at: 4327 4694 4327 Size: 367 6630 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4318 5773 618 Size: 325 217 627 2531 1455 1842 Fragments arranged by size: 2531 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2379 3114 4316 1391 Size: 988 735 1202 4072 Fragments arranged by size: 4072 1202 988 735 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3058 3192 3237 928 Size: 971 1159 134 45 4688 Fragments arranged by size: 4688 1159 971 134 45 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5188 5222 1903 Size: 34 3251 34 3678 Fragments arranged by size: 3678 3251 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3031 4889 6099 3031 Size: 1858 1210 3929 Fragments arranged by size: 3929 1858 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6997 BfrBI ATG'CAT Cuts at: 2216 2663 3912 2216 Size: 447 1249 5301 Fragments arranged by size: 5301 1249 447 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5608 136 Size: 107 122 71 631 605 3936 1525 Fragments arranged by size: 3936 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3813 6992 3813 Size: 3179 3818 BlpI GC'TnA_GC Cuts at: 4414 4414 Size: 6997 Bme1580I G_kGCm'C Cuts at: 1790 5058 6214 1790 Size: 3268 1156 2573 Fragments arranged by size: 3268 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2701 1318 Size: 1383 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2154 4728 5686 Size: 416 53 83 399 24 737 2574 958 Cuts at: 5686 442 Size: 1753 Fragments arranged by size: 2574 1753 958 737 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4155 4155 Size: 6997 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2762 3575 5657 1239 Size: 1523 813 2082 2579 Fragments arranged by size: 2579 2082 1523 813 Bpu10I CC'TnA_GC Cuts at: 4113 4113 Size: 6997 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2824 2912 5122 5987 6285 2824 Size: 88 2210 865 298 3536 Fragments arranged by size: 3536 2210 865 298 88 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3722 4202 5660 882 Size: 617 2223 480 1458 2219 Fragments arranged by size: 2223 2219 1458 617 480 BsaAI yAC'GTr Cuts at: 493 1204 2251 4788 493 Size: 711 1047 2537 2702 Fragments arranged by size: 2702 2537 1047 711 BsaBI GATnn'nnATC Cuts at: 3818 6991 3818 Size: 3173 3824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2498 4804 5186 5821 Size: 53 83 186 936 965 2306 382 635 Cuts at: 5821 275 Size: 1451 Fragments arranged by size: 2306 1451 965 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3594 5430 5828 6102 6249 6660 3594 Size: 1836 398 274 147 411 3931 Fragments arranged by size: 3931 1836 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3548 3578 1178 Size: 30 165 30 2145 30 4597 Fragments arranged by size: 4597 2145 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2685 3311 3398 1186 Size: 934 565 626 87 4785 Fragments arranged by size: 4785 934 626 565 87 BseYI C'CCAG_C Cuts at: 2147 3639 6200 6696 2147 Size: 1492 2561 496 2448 Fragments arranged by size: 2561 2448 1492 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4447 5208 6236 1540 Size: 275 186 2446 761 1028 2301 Fragments arranged by size: 2446 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 2748 3114 5058 5143 6214 2379 Size: 369 366 1944 85 1071 3162 Fragments arranged by size: 3162 1944 1071 369 366 85 BsmI GAATG_Cn' Cuts at: 2518 2956 4508 4601 2518 Size: 438 1552 93 4914 Fragments arranged by size: 4914 1552 438 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2085 2085 Size: 6997 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2379 2748 3114 4316 5058 5143 6214 Size: 399 589 369 366 1202 742 85 1071 Cuts at: 6214 1391 Size: 2174 Fragments arranged by size: 2174 1202 1071 742 589 399 369 366 85 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 4886 6616 4886 Size: 1730 5267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 2132 3411 4352 844 Size: 1211 77 1279 941 3489 Fragments arranged by size: 3489 1279 1211 941 77 BsrBI CCG'CTC Cuts at: 1394 1823 4884 1394 Size: 429 3061 3507 Fragments arranged by size: 3507 3061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 3304 5492 5666 65 Size: 1183 1291 765 2188 174 1396 Fragments arranged by size: 2188 1396 1291 1183 765 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 5641 5828 1700 Size: 110 201 3630 187 2869 Fragments arranged by size: 3630 2869 201 187 110 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 4537 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6997 BssSI C'ACGA_G Cuts at: 5057 6069 5057 Size: 1012 5985 BstAPI GCAn_nnn'nTGC Cuts at: 794 2032 2929 794 Size: 1238 897 4862 Fragments arranged by size: 4862 1238 897 BstBI TT'CG_AA Cuts at: 5801 5801 Size: 6997 BstEII G'GTnAC_C Cuts at: 1184 4174 1184 Size: 2990 4007 BstXI CCAn_nnnn'nTGG Cuts at: 2059 2740 3589 3880 2059 Size: 681 849 291 5176 Fragments arranged by size: 5176 849 681 291 BstYI r'GATC_y Cuts at: 1972 3600 3813 4327 4694 5079 5096 6548 6634 Size: 1628 213 514 367 385 17 1452 86 Cuts at: 6634 6646 6992 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1628 1452 514 385 367 346 213 86 17 12 BstZ17I GTA'TAC Cuts at: 2677 3534 2677 Size: 857 6140 Bsu36I CC'TnA_GG Cuts at: 3608 3608 Size: 6997 BtgI C'CryG_G Cuts at: 513 1065 3717 513 Size: 552 2652 3793 Fragments arranged by size: 3793 2652 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3397 487 Size: 683 56 2171 4087 Fragments arranged by size: 4087 2171 683 56 BtsI GCAGTG_nn' Cuts at: 2718 2848 4513 5307 5327 2718 Size: 130 1665 794 20 4388 Fragments arranged by size: 4388 1665 794 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6997 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2784 2819 529 Size: 35 2220 35 4707 Fragments arranged by size: 4707 2220 35 35 DraI TTT'AAA Cuts at: 4301 4301 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2117 6004 809 Size: 507 801 3887 1802 Fragments arranged by size: 3887 1802 801 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 4444 5333 Size: 54 1425 50 275 18 1273 1341 889 Cuts at: 5333 6962 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1341 1273 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4444 1537 Size: 275 2632 4090 Fragments arranged by size: 4090 2632 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 3123 4004 4269 4927 1353 Size: 1626 144 881 265 658 3423 Fragments arranged by size: 3423 1626 881 658 265 144 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2079 3593 5584 5958 6104 Size: 1064 138 441 49 1514 1991 374 146 Cuts at: 6104 387 Size: 1280 Fragments arranged by size: 1991 1514 1280 1064 441 374 146 138 49 EcoICRI GAG'CTC Cuts at: 2377 3112 2377 Size: 735 6262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2195 2559 2570 2762 2771 3575 Size: 393 466 97 364 11 192 9 804 Cuts at: 3575 5060 5657 6444 6712 1239 Size: 1485 597 787 268 1524 Fragments arranged by size: 1524 1485 804 787 597 466 393 364 268 192 97 11 9 EcoNI CCTnn'n_nnAGG Cuts at: 2972 4060 2972 Size: 1088 5909 EcoO109I rG'GnC_Cy Cuts at: 2056 3476 2056 Size: 1420 5577 EcoRI G'AATT_C Cuts at: 4306 4684 4306 Size: 378 6619 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 3562 6144 1678 Size: 1884 2582 2531 Fragments arranged by size: 2582 2531 1884 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4180 4212 5638 5670 5712 Size: 32 135 32 2608 32 1426 32 42 Cuts at: 5712 5744 1373 Size: 32 2626 Fragments arranged by size: 2626 2608 1426 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4341 4587 669 Size: 988 281 2403 246 3079 Fragments arranged by size: 3079 2403 988 281 246 HindIII A'AGCT_T Cuts at: 748 2177 2689 3141 748 Size: 1429 512 452 4604 Fragments arranged by size: 4604 1429 512 452 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KpnI G_GTAC'C Cuts at: 4322 4322 Size: 6997 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6111 6295 1305 Size: 4806 184 2007 Fragments arranged by size: 4806 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3105 10 Size: 54 1425 343 1273 3902 Fragments arranged by size: 3902 1425 1273 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2853 3395 3618 3911 4955 5314 5473 Size: 648 1687 542 223 293 1044 359 159 Cuts at: 5473 518 Size: 2042 Fragments arranged by size: 2042 1687 1044 648 542 359 293 223 159 MspA1I CmG'CkG Cuts at: 1606 2029 2278 4413 5090 6238 6483 1606 Size: 423 249 2135 677 1148 245 2120 Fragments arranged by size: 2135 2120 1148 677 423 249 245 NaeI GCC'GGC Cuts at: 2013 2013 Size: 6997 NcoI C'CATG_G Cuts at: 513 1065 3717 513 Size: 552 2652 3793 Fragments arranged by size: 3793 2652 552 NdeI CA'TA_TG Cuts at: 387 3170 3908 387 Size: 2783 738 3476 Fragments arranged by size: 3476 2783 738 NgoMIV G'CCGG_C Cuts at: 2011 2011 Size: 6997 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NsiI A_TGCA'T Cuts at: 2218 2665 3914 2218 Size: 447 1249 5301 Fragments arranged by size: 5301 1249 447 NspI r_CATG'y Cuts at: 1132 1229 3621 4355 5900 1132 Size: 97 2392 734 1545 2229 Fragments arranged by size: 2392 2229 1545 734 97 PasI CC'CwG_GG Cuts at: 1348 2052 3479 3848 1348 Size: 704 1427 369 4497 Fragments arranged by size: 4497 1427 704 369 PciI A'CATG_T Cuts at: 5896 5896 Size: 6997 PflMI CCAn_nnn'nTGG Cuts at: 1108 3465 4207 1108 Size: 2357 742 3898 Fragments arranged by size: 3898 2357 742 PfoI T'CCnGG_A Cuts at: 3033 3033 Size: 6997 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5066 5098 6579 6611 5066 Size: 32 1481 32 5452 Fragments arranged by size: 5452 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2056 Size: 6997 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2333 2365 2333 Size: 32 6965 PstI C_TGCA'G Cuts at: 830 2028 2274 4349 830 Size: 1198 246 2075 3478 Fragments arranged by size: 3478 2075 1198 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 PvuII CAG'CTG Cuts at: 2029 2278 2029 Size: 249 6748 SacI G_AGCT'C Cuts at: 2379 3114 2379 Size: 735 6262 SalI G'TCGA_C Cuts at: 1936 4339 1936 Size: 2403 4594 SbfI CC_TGCA'GG Cuts at: 4349 4349 Size: 6997 ScaI AGT'ACT Cuts at: 3446 5245 6957 3446 Size: 1799 1712 3486 Fragments arranged by size: 3486 1799 1712 SfcI C'TryA_G Cuts at: 826 1045 2024 2256 2270 3420 4345 5480 6161 Size: 219 979 232 14 1150 925 1135 681 Cuts at: 6161 6352 826 Size: 191 1471 Fragments arranged by size: 1471 1150 1135 979 925 681 232 219 191 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 4324 1872 Size: 2452 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2783 2803 2891 5101 6002 6264 Size: 197 926 840 20 88 2210 901 262 Cuts at: 6264 820 Size: 1553 Fragments arranged by size: 2210 1553 926 901 840 262 197 88 20 SnaBI TAC'GTA Cuts at: 493 2251 4788 493 Size: 1758 2537 2702 Fragments arranged by size: 2702 2537 1758 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 2236 4921 5 Size: 47 2184 2685 2081 Fragments arranged by size: 2685 2184 2081 47 StuI AGG'CCT Cuts at: 1517 2739 4055 1517 Size: 1222 1316 4459 Fragments arranged by size: 4459 1316 1222 StyI C'CwwG_G Cuts at: 513 1065 2395 2581 3717 4050 4436 513 Size: 552 1330 186 1136 333 386 3074 Fragments arranged by size: 3074 1330 1136 552 386 333 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2984 3471 5024 5041 5194 5379 5824 Size: 1255 1404 487 1553 17 153 185 445 Cuts at: 5824 325 Size: 1498 Fragments arranged by size: 1553 1498 1404 1255 487 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 3444 3840 Size: 274 80 33 51 1443 579 888 396 Cuts at: 3840 5243 6955 96 Size: 1403 1712 138 Fragments arranged by size: 1712 1443 1403 888 579 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2710 2742 6957 6989 2710 Size: 32 4215 32 2718 Fragments arranged by size: 4215 2718 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6997 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3988 3988 Size: 6997 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6997 XmaI C'CCGG_G Cuts at: 1870 4322 1870 Size: 2452 4545 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2499 4805 276 Size: 53 83 186 936 965 2306 2468 Fragments arranged by size: 2468 2306 965 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI