Restriction map for pFN21AB3962
 (Circular) (Six-base) MAPSORT of: pFN21AB3962.seq  Check: 9428  from: 1  to: 6220



pFN21AB3962  6220 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:35  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4030     278 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2432    3563    1937 
   Size:        495    1131    4594

  Fragments arranged by size: 

                4594    1131     495
 
Acc65I G'GTAC_C

Cuts at:   3541    3541 
   Size:       6220
 
AclI AA'CG_TT

Cuts at:   1563    4347    4720    1563 
   Size:       2784     373    3063

  Fragments arranged by size: 

                3063    2784     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2286    3177    3411    4283    5667    5935    1632 
   Size:        654     891     234     872    1384     268    1917

  Fragments arranged by size: 

                1917    1384     891     872     654     268     234
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6023
 
AflIII A'CryG_T

Cuts at:   1203    2020    5119    6096    1203 
   Size:        817    3099     977    1327

  Fragments arranged by size: 

                3099    1327     977     817
 
AgeI A'CCGG_T

Cuts at:   5051    5051 
   Size:       6220
 
AhdI GACnn_n'nnGTC

Cuts at:   4949    4949 
   Size:       6220
 
AleI CACnn'nnGTG

Cuts at:   1166    2765    1166 
   Size:       1599    4621
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6220
 
AlwNI CAG_nnn'CTG

Cuts at:   3416    3464    3963    5535    3416 
   Size:         48     499    1572    4101

  Fragments arranged by size: 

                4101    1572     499      48
 
ApaLI G'TGCA_C

Cuts at:   4277    5433    4277 
   Size:       1156    5064
 
ApoI r'AATT_y

Cuts at:   1493    2009    2026    2165    2288    3529    3755    3907    6016 
   Size:        516      17     139     123    1241     226     152    2109

Cuts at:   6016    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697    1241     516     226     152     139     123
                  17
 
AseI AT'TA_AT

Cuts at:    160    1032    4774     160 
   Size:        872    3742    1606

  Fragments arranged by size: 

                3742    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3545    1870 
   Size:         73    1602    4545

  Fragments arranged by size: 

                4545    1602      73
 
BamHI G'GATC_C

Cuts at:   3550    3917    3550 
   Size:        367    5853
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2605    2767    3541    4996     618 
   Size:        325     217     627     818     162     774    1455    1842

  Fragments arranged by size: 

                1842    1455     818     774     627     325     217     162
            
 
BanII G_rGCy'C

Cuts at:   1391    3539    1391 
   Size:       2148    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3165    3327     928 
   Size:        971    1266     162    3821

  Fragments arranged by size: 

                3821    1266     971     162
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4411    4445    1903 
   Size:         34    2474      34    3678

  Fragments arranged by size: 

                3678    2474      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4112    5322    4112 
   Size:       1210    5010
 
BclI T'GATC_A

Cuts at:   1548    2203    1548 
   Size:        655    5565
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4831     136 
   Size:        107     122      71     631     605    3159    1525

  Fragments arranged by size: 

                3159    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6215    6215 
   Size:       6220
 
BlpI GC'TnA_GC

Cuts at:   3637    3637 
   Size:       6220
 
Bme1580I G_kGCm'C

Cuts at:   1790    4281    5437    1790 
   Size:       2491    1156    2573

  Fragments arranged by size: 

                2573    2491    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6220
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3493    3951    4909 
   Size:        416      53      83     399      24    2076     458     958

Cuts at:   4909     442 
   Size:       1753

  Fragments arranged by size: 

                2076    1753     958     458     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6220
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2850    2973    4880    1239 
   Size:       1611     123    1907    2579

  Fragments arranged by size: 

                2579    1907    1611     123
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2530    2569    3184    4345    5210    5508    2530 
   Size:         39     615    1161     865     298    3242

  Fragments arranged by size: 

                3242    1161     865     615     298      39
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4883     882 
   Size:        617    3384    2219

  Fragments arranged by size: 

                3384    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2180    4011     493 
   Size:        711     976    1831    2702

  Fragments arranged by size: 

                2702    1831     976     711
 
BsaBI GATnn'nnATC

Cuts at:   6214    6214 
   Size:       6220
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4027    4409    5044     275 
   Size:         53      83     186     936    2494     382     635    1451

  Fragments arranged by size: 

                2494    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4653    5051    5325    5472    5883    4653 
   Size:        398     274     147     411    4990

  Fragments arranged by size: 

                4990     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2962    2992    1178 
   Size:         30     165      30    1559      30    4406

  Fragments arranged by size: 

                4406    1559     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2390    1186 
   Size:       1204    5016
 
BseYI C'CCAG_C

Cuts at:   2853    5423    5919    2853 
   Size:       2570     496    3154

  Fragments arranged by size: 

                3154    2570     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2457    1163 
   Size:        573     721    4926

  Fragments arranged by size: 

                4926     721     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2939    3670    4431    5459    1540 
   Size:        275     186     938     731     761    1028    2301

  Fragments arranged by size: 

                2301    1028     938     761     731     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4281    4366    5437    4281 
   Size:         85    1071    5064

  Fragments arranged by size: 

                5064    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3731    3824    3731 
   Size:         93    6127
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3539    4281    4366    5437    1391 
   Size:        399    1749     742      85    1071    2174

  Fragments arranged by size: 

                2174    1749    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5883    5883 
   Size:       6220
 
BspHI T'CATG_A

Cuts at:   4109    5839    4109 
   Size:       1730    4490
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3575     844 
   Size:       2731    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4107    1394 
   Size:        429    2284    3507

  Fragments arranged by size: 

                3507    2284     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4715    4889      65 
   Size:       1183    3467     174    1396

  Fragments arranged by size: 

                3467    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4864    5051    1700 
   Size:        110    3054     187    2869

  Fragments arranged by size: 

                3054    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6220
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6220
 
BssSI C'ACGA_G

Cuts at:   4280    5292    4280 
   Size:       1012    5208
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2471     794 
   Size:       1677    4543
 
BstBI TT'CG_AA

Cuts at:   5024    5024 
   Size:       6220
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6220
 
BstYI r'GATC_y

Cuts at:   1972    3052    3550    3917    4302    4319    5771    5857    5869 
   Size:       1080     498     367     385      17    1452      86      12

Cuts at:   5869    6215    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452    1080     498     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3511    3511 
   Size:       6220
 
BtgI C'CryG_G

Cuts at:    513    1065    2763     513 
   Size:        552    1698    3970

  Fragments arranged by size: 

                3970    1698     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5481

  Fragments arranged by size: 

                5481     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3736    4530    4550    3736 
   Size:        794      20    5406

  Fragments arranged by size: 

                5406     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6220
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6185
 
DraI TTT'AAA

Cuts at:   3524    3524 
   Size:       6220
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5227     809 
   Size:        507    3911    1802

  Fragments arranged by size: 

                3911    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2101    3667    4556 
   Size:         54    1425      50     275      18     271    1566     889

Cuts at:   4556    6185       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1566    1425     889     275     271      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3667    1537 
   Size:        275    1855    4090

  Fragments arranged by size: 

                4090    1855     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2658    3031    4150    1353 
   Size:       1305     373    1119    3423

  Fragments arranged by size: 

                3423    1305    1119     373
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3461    4807    5181    5327     387 
   Size:       1064     138    1872    1346     374     146    1280

  Fragments arranged by size: 

                1872    1346    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2286    2850    2973    3177    3411    4283    4880 
   Size:        393     654     564     123     204     234     872     597

Cuts at:   4880    5667    5935    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     872     787     654     597     564     393     268
                 234     204     123
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2617    2637    2617 
   Size:         20    6200
 
EcoO109I rG'GnC_Cy

Cuts at:   2047    2792    2047 
   Size:        745    5475
 
EcoRI G'AATT_C

Cuts at:   2026    2288    3529    3907    2026 
   Size:        262    1241     378    4339

  Fragments arranged by size: 

                4339    1241     378     262
 
FspI TGC'GCA

Cuts at:   4726    4726 
   Size:       6220
 
HaeII r_GCGC'y

Cuts at:   1678    5367    1678 
   Size:       3689    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4861    4893    4935    4967    1373 
   Size:         32     135      32    3289      32      42      32    2626

  Fragments arranged by size: 

                3289    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3564    3810     669 
   Size:        988     281    1626     246    3079

  Fragments arranged by size: 

                3079    1626     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2298     748 
   Size:       1550    4670
 
HpaI GTT'AAC

Cuts at:   3810    3810 
   Size:       6220
 
KpnI G_GTAC'C

Cuts at:   3545    3545 
   Size:       6220
 
MfeI C'AATT_G

Cuts at:   2625    3819    2625 
   Size:       1194    5026
 
MluI A'CGCG_T

Cuts at:   6096    6096 
   Size:       6220
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3011    5334    5518    1305 
   Size:       1706    2323     184    2007

  Fragments arranged by size: 

                2323    2007    1706     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2103      10 
   Size:         54    1425     343     271    4127

  Fragments arranged by size: 

                4127    1425     343     271      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2148    2765    3329    3440    4178    4537    4696 
   Size:        648     982     617     564     111     738     359     159

Cuts at:   4696     518 
   Size:       2042

  Fragments arranged by size: 

                2042     982     738     648     617     564     359     159
                 111
 
MspA1I CmG'CkG

Cuts at:   1606    2735    3184    3226    3636    4313    5461    5706    1606 
   Size:       1129     449      42     410     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1129     677     449     410     245      42
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2763     513 
   Size:        552    1698    3970

  Fragments arranged by size: 

                3970    1698     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6220
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6220
 
NotI GC'GGCC_GC

Cuts at:   3667    3667 
   Size:       6220
 
NspI r_CATG'y

Cuts at:   1132    1229    2024    2729    3578    5123    1132 
   Size:         97     795     705     849    1545    2229

  Fragments arranged by size: 

                2229    1545     849     795     705      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6220
 
PciI A'CATG_T

Cuts at:   2020    5119    2020 
   Size:       3099    3121
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6220
 
PfoI T'CCnGG_A

Cuts at:   2779    2779 
   Size:       6220
 
PmeI GTTT'AAAC

Cuts at:   3524    3524 
   Size:       6220
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4289    4321    5802    5834    4289 
   Size:         32    1481      32    4675

  Fragments arranged by size: 

                4675    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2047    2792    2047 
   Size:        745    5475
 
PshAI GACnn'nnGTC

Cuts at:   1613    2941    1613 
   Size:       1328    4892
 
PsiI TTA'TAA

Cuts at:   3790    3790 
   Size:       6220
 
PstI C_TGCA'G

Cuts at:    830    2740    3189    3572     830 
   Size:       1910     449     383    3478

  Fragments arranged by size: 

                3478    1910     449     383
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6220
 
PvuII CAG'CTG

Cuts at:   2735    3184    3226    2735 
   Size:        449      42    5729

  Fragments arranged by size: 

                5729     449      42
 
SalI G'TCGA_C

Cuts at:   1936    3562    1936 
   Size:       1626    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3031    3031 
   Size:       6220
 
SbfI CC_TGCA'GG

Cuts at:   3572    3572 
   Size:       6220
 
ScaI AGT'ACT

Cuts at:   2590    4468    6180    2590 
   Size:       1878    1712    2630

  Fragments arranged by size: 

                2630    1878    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2736    2970    3185    3417    3568    4703    5384 
   Size:        219    1691     234     215     232     151    1135     681

Cuts at:   5384    5575     826 
   Size:        191    1471

  Fragments arranged by size: 

                1691    1471    1135     681     234     232     219     215
                 191     151
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6220
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6220
 
SmaI CCC'GGG

Cuts at:   1872    3547    1872 
   Size:       1675    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2545    2584    3163    4324    5225    5487 
   Size:        197     926     602      39     579    1161     901     262

Cuts at:   5487     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1161     926     901     602     579     262     197
                  39
 
SnaBI TAC'GTA

Cuts at:    493    4011     493 
   Size:       3518    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6220
 
SphI G_CATG'C

Cuts at:   1132    3578    1132 
   Size:       2446    3774
 
SspI AAT'ATT

Cuts at:      5      52    4144       5 
   Size:         47    4092    2081

  Fragments arranged by size: 

                4092    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6220
 
StyI C'CwwG_G

Cuts at:    513    1065    2763    3405    3659     513 
   Size:        552    1698     642     254    3074

  Fragments arranged by size: 

                3074    1698     642     552     254
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2439    2615    3361    3452    4247    4264    4417 
   Size:       1255     859     176     746      91     795      17     153

Cuts at:   4417    4602    5047     325 
   Size:        185     445    1498

  Fragments arranged by size: 

                1498    1255     859     795     746     445     185     176
                 153      91      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2084    2588    2812 
   Size:        274      80      33      51    1443     107     504     224

Cuts at:   2812    3017    3455    4466    6178      96 
   Size:        205     438    1011    1712     138

  Fragments arranged by size: 

                1712    1443    1011     504     438     274     224     205
                 138     107      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2047    2079    6180    6212    2047 
   Size:         32    4101      32    2055

  Fragments arranged by size: 

                4101    2055      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2189    2920    1316 
   Size:        873     731    4616

  Fragments arranged by size: 

                4616     873     731
 
XbaI T'CTAG_A

Cuts at:   2329    3556    2329 
   Size:       1227    4993
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3020    3020 
   Size:       6220
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6220
 
XmaI C'CCGG_G

Cuts at:   1870    3545    1870 
   Size:       1675    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2288    4349    2288 
   Size:       2061    4159
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4028     276 
   Size:         53      83     186     936    2494    2468

  Fragments arranged by size: 

                2494    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   DraIII  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI
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