(Circular) (Six-base) MAPSORT of: pFN21AB3962.seq Check: 9428 from: 1 to: 6220 pFN21AB3962 6220 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2432 3563 1937 Size: 495 1131 4594 Fragments arranged by size: 4594 1131 495 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3177 3411 4283 5667 5935 1632 Size: 654 891 234 872 1384 268 1917 Fragments arranged by size: 1917 1384 891 872 654 268 234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2020 5119 6096 1203 Size: 817 3099 977 1327 Fragments arranged by size: 3099 1327 977 817 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 2765 1166 Size: 1599 4621 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 3416 3464 3963 5535 3416 Size: 48 499 1572 4101 Fragments arranged by size: 4101 1572 499 48 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2009 2026 2165 2288 3529 3755 3907 6016 Size: 516 17 139 123 1241 226 152 2109 Cuts at: 6016 1493 Size: 1697 Fragments arranged by size: 2109 1697 1241 516 226 152 139 123 17 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2605 2767 3541 4996 618 Size: 325 217 627 818 162 774 1455 1842 Fragments arranged by size: 1842 1455 818 774 627 325 217 162 BanII G_rGCy'C Cuts at: 1391 3539 1391 Size: 2148 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3165 3327 928 Size: 971 1266 162 3821 Fragments arranged by size: 3821 1266 971 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 2203 1548 Size: 655 5565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 4281 5437 1790 Size: 2491 1156 2573 Fragments arranged by size: 2573 2491 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3493 3951 4909 Size: 416 53 83 399 24 2076 458 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 2076 1753 958 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2850 2973 4880 1239 Size: 1611 123 1907 2579 Fragments arranged by size: 2579 1907 1611 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2530 2569 3184 4345 5210 5508 2530 Size: 39 615 1161 865 298 3242 Fragments arranged by size: 3242 1161 865 615 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2180 4011 493 Size: 711 976 1831 2702 Fragments arranged by size: 2702 1831 976 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2962 2992 1178 Size: 30 165 30 1559 30 4406 Fragments arranged by size: 4406 1559 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 1186 Size: 1204 5016 BseYI C'CCAG_C Cuts at: 2853 5423 5919 2853 Size: 2570 496 3154 Fragments arranged by size: 3154 2570 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2457 1163 Size: 573 721 4926 Fragments arranged by size: 4926 721 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2939 3670 4431 5459 1540 Size: 275 186 938 731 761 1028 2301 Fragments arranged by size: 2301 1028 938 761 731 275 186 BsiHKAI G_wGCw'C Cuts at: 4281 4366 5437 4281 Size: 85 1071 5064 Fragments arranged by size: 5064 1071 85 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3539 4281 4366 5437 1391 Size: 399 1749 742 85 1071 2174 Fragments arranged by size: 2174 1749 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 2471 794 Size: 1677 4543 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstYI r'GATC_y Cuts at: 1972 3052 3550 3917 4302 4319 5771 5857 5869 Size: 1080 498 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1080 498 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3511 3511 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2763 513 Size: 552 1698 3970 Fragments arranged by size: 3970 1698 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 3736 4530 4550 3736 Size: 794 20 5406 Fragments arranged by size: 5406 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 3667 4556 Size: 54 1425 50 275 18 271 1566 889 Cuts at: 4556 6185 8 Size: 1629 43 Fragments arranged by size: 1629 1566 1425 889 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 3031 4150 1353 Size: 1305 373 1119 3423 Fragments arranged by size: 3423 1305 1119 373 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3461 4807 5181 5327 387 Size: 1064 138 1872 1346 374 146 1280 Fragments arranged by size: 1872 1346 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2850 2973 3177 3411 4283 4880 Size: 393 654 564 123 204 234 872 597 Cuts at: 4880 5667 5935 1239 Size: 787 268 1524 Fragments arranged by size: 1524 872 787 654 597 564 393 268 234 204 123 EcoNI CCTnn'n_nnAGG Cuts at: 2617 2637 2617 Size: 20 6200 EcoO109I rG'GnC_Cy Cuts at: 2047 2792 2047 Size: 745 5475 EcoRI G'AATT_C Cuts at: 2026 2288 3529 3907 2026 Size: 262 1241 378 4339 Fragments arranged by size: 4339 1241 378 262 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373 Size: 32 135 32 3289 32 42 32 2626 Fragments arranged by size: 3289 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 2298 748 Size: 1550 4670 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 2625 3819 2625 Size: 1194 5026 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3011 5334 5518 1305 Size: 1706 2323 184 2007 Fragments arranged by size: 2323 2007 1706 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2103 10 Size: 54 1425 343 271 4127 Fragments arranged by size: 4127 1425 343 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2765 3329 3440 4178 4537 4696 Size: 648 982 617 564 111 738 359 159 Cuts at: 4696 518 Size: 2042 Fragments arranged by size: 2042 982 738 648 617 564 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2735 3184 3226 3636 4313 5461 5706 1606 Size: 1129 449 42 410 677 1148 245 2120 Fragments arranged by size: 2120 1148 1129 677 449 410 245 42 NcoI C'CATG_G Cuts at: 513 1065 2763 513 Size: 552 1698 3970 Fragments arranged by size: 3970 1698 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2024 2729 3578 5123 1132 Size: 97 795 705 849 1545 2229 Fragments arranged by size: 2229 1545 849 795 705 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 2020 5119 2020 Size: 3099 3121 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6220 PfoI T'CCnGG_A Cuts at: 2779 2779 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2792 2047 Size: 745 5475 PshAI GACnn'nnGTC Cuts at: 1613 2941 1613 Size: 1328 4892 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PstI C_TGCA'G Cuts at: 830 2740 3189 3572 830 Size: 1910 449 383 3478 Fragments arranged by size: 3478 1910 449 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2735 3184 3226 2735 Size: 449 42 5729 Fragments arranged by size: 5729 449 42 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SapI GCTCTTCn'nnn_ Cuts at: 3031 3031 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 2590 4468 6180 2590 Size: 1878 1712 2630 Fragments arranged by size: 2630 1878 1712 SfcI C'TryA_G Cuts at: 826 1045 2736 2970 3185 3417 3568 4703 5384 Size: 219 1691 234 215 232 151 1135 681 Cuts at: 5384 5575 826 Size: 191 1471 Fragments arranged by size: 1691 1471 1135 681 234 232 219 215 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2545 2584 3163 4324 5225 5487 Size: 197 926 602 39 579 1161 901 262 Cuts at: 5487 820 Size: 1553 Fragments arranged by size: 1553 1161 926 901 602 579 262 197 39 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2763 3405 3659 513 Size: 552 1698 642 254 3074 Fragments arranged by size: 3074 1698 642 552 254 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2439 2615 3361 3452 4247 4264 4417 Size: 1255 859 176 746 91 795 17 153 Cuts at: 4417 4602 5047 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 859 795 746 445 185 176 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2084 2588 2812 Size: 274 80 33 51 1443 107 504 224 Cuts at: 2812 3017 3455 4466 6178 96 Size: 205 438 1011 1712 138 Fragments arranged by size: 1712 1443 1011 504 438 274 224 205 138 107 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2047 2079 6180 6212 2047 Size: 32 4101 32 2055 Fragments arranged by size: 4101 2055 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2189 2920 1316 Size: 873 731 4616 Fragments arranged by size: 4616 873 731 XbaI T'CTAG_A Cuts at: 2329 3556 2329 Size: 1227 4993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3020 3020 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2288 4349 2288 Size: 2061 4159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI