Restriction map for
pFN21AB3619
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI Hin1I HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 1137 ACACGT A^C[GA][CT]GT
AgeI 1454 ACCGGT A^CCGGT
AlwNI 240 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2607 CCTGAGG CC^T[ACGT]AGG
AseI 1318 ATTAAT AT^TAAT
AsnI 1318 ATTAAT AT^TAAT
Asp700I 1963 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1944 CTCGGG C^[CT]CG[GA]G
BalI 753 TGGCCA TGG^CCA
BciVI 2319 GGATAC GTATCC(6/5)
BglII 2213 AGATCT A^GATCT
BsaBI 796 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1133 CTGCAC GTGCAG(16/14)
BsiEI 17 CGGTCG CG[GA][CT]^CG
BsoBI 1944 CTCGGG C^[CT]CG[GA]G
BspHI 1235 TCATGA T^CATGA
BsrBI 10 CCGCTC CCGCTC(-3/-3)
BsrFI 1454 ACCGGT [GA]^CCGG[CT]
Bsu36I 2607 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1454 ACCGGT [GA]^CCGG[CT]
DraIII 2356 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 753 TGGCCA [CT]^GGCC[GA]
Eco81I 2607 CCTGAGG CC^T[ACGT]AGG
EcoNI 2133 CCTAGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1502 GAATTC G^AATTC
HgaI 2564 GCGTC GACGC(5/10)
MamI 796 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 753 TGGCCA TGG^CCA
MscI 753 TGGCCA TGG^CCA
MslI 2497 CATAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PinAI 1454 ACCGGT A^CCGGT
PshBI 1318 ATTAAT AT^TAAT
PvuII 329 CAGCTG CAG^CTG
RcaI 1235 TCATGA T^CATGA
SacI 2293 GAGCTC GAGCT^C
SapI 2290 GAAGAGC GCTCTTC(1/4)
ScaI 1829 AGTACT AGT^ACT
SmlI 843 CTTGAG C^T[CT][GA]AG
SpeI 2596 ACTAGT A^CTAGT
SphI 1286 GCATGC GCATG^C
SspI 1363 AATATT AAT^ATT
StuI 2611 AGGCCT AGG^CCT
XmnI 1963 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 398 GGTACC G^GTACC
1628 GGTACC
Asp718I 398 GGTACC G^GTACC
1628 GGTACC
AspHI 846 GAGCAC G[AT]GC[AT]^C
2293 GAGCTC
BbrPI 569 CACGTG CAC^GTG
1138 CACGTG
BpmI 761 CTCCAG CTGGAG(16/14)
2296 CTCCAG
BsaAI 569 CACGTG [CT]AC^GT[GA]
1138 CACGTG
BsaI 2190 GAGACC GGTCTC(1/5)
664 GGTCTC
BsaWI 1454 ACCGGT [AT]^CCGG[AT]
1631 ACCGGA
BsiHKAI 846 GAGCAC G[AT]GC[AT]^C
2293 GAGCTC
BsmFI 266 GGGAC GGGAC(10/14)
1204 GTCCC
BstXI 2271 CCAGACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2621 CCAACCTTATGG
BstYI 2213 AGATCT [GA]^GATC[CT]
2459 GGATCT
DraII 66 GGGTCCT [GA]G^G[ACGT]CC[CT]
2261 AGGACCT
DsaI 896 CCATGG C^C[GA][CT]GG
1840 CCATGG
EcoO109I 66 GGGTCCT [GA]G^G[ACGT]CC[CT]
2261 AGGACCT
EcoRV 163 GATATC GAT^ATC
811 GATATC
HaeII 281 AGCGCC [GA]GCGC^[CT]
1585 AGCGCC
HaeIII 754 GGCC GG^CC
2612 GGCC
HincII 706 GTCAAC GT[CT]^[GA]AC
1393 GTTGAC
HindII 706 GTCAAC GT[CT]^[GA]AC
1393 GTTGAC
HindIII 581 AAGCTT A^AGCTT
1385 AAGCTT
KpnI 398 GGTACC GGTAC^C
1628 GGTACC
MaeII 570 ACGT A^CGT
1139 ACGT
MflI 2213 AGATCT [GA]^GATC[CT]
2459 GGATCT
MspA1I 329 CAGCTG C[AC]G^C[GT]G
655 CAGCGG
NcoI 896 CCATGG C^CATGG
1840 CCATGG
NspI 153 GCATGT [GA]CATG^[CT]
1286 GCATGC
PflMI 831 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2355 CCACTCTGTGG
PmaCI 569 CACGTG CAC^GTG
1138 CACGTG
PmlI 569 CACGTG CAC^GTG
1138 CACGTG
PpuMI 66 GGGTCCT [GA]G^G[AT]CC[CT]
2261 AGGACCT
SfcI 902 CTGTAG C^T[GA][CT]AG
1022 CTACAG
Van91I 831 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2355 CCACTCTGTGG
XhoII 2213 AGATCT [GA]^GATC[CT]
2459 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I