Restriction map for
pFN21AB3619
(Circular) (Six-base) MAPSORT of: pFN21AB3619.seq Check: 6558 from: 1 to: 7411
pFN21AB3619 7411 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5221 278
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4754 1937
Size: 2817 4594
Acc65I G'GTAC_C
Cuts at: 2403 3633 4732 2403
Size: 1230 1099 5082
Fragments arranged by size:
5082 1230 1099
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 3323 4187 4392 5474 6858 7126 1632
Size: 544 1147 864 205 1082 1384 268 1917
Fragments arranged by size:
1917 1384 1147 1082 864 544 268 205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 3142 6310 7287 1203
Size: 1939 3168 977 1327
Fragments arranged by size:
3168 1939 1327 977
AgeI A'CCGG_T
Cuts at: 3459 6242 3459
Size: 2783 4628
AhdI GACnn_n'nnGTC
Cuts at: 6140 6140
Size: 7411
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7411
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 4256 4288 3407
Size: 32 817 32 6530
Fragments arranged by size:
6530 817 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2250 5154 6726 2250
Size: 2904 1572 2935
Fragments arranged by size:
2935 2904 1572
ApaLI G'TGCA_C
Cuts at: 5468 6624 5468
Size: 1156 6255
ApoI r'AATT_y
Cuts at: 1493 2256 2482 2500 3353 3507 4550 4720 4946
Size: 763 226 18 853 154 1043 170 226
Cuts at: 4946 5098 7207 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1043 853 763 226 226 170
154 152 18
AseI AT'TA_AT
Cuts at: 160 1032 3324 5965 160
Size: 872 2292 2641 1606
Fragments arranged by size:
2641 2292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 3949 4736 1870
Size: 73 2006 787 4545
Fragments arranged by size:
4545 2006 787 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3001 3034 3617 3650 3001
Size: 33 583 33 6762
Fragments arranged by size:
6762 583 33 33
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2403 3633 4184 4732 6187
Size: 325 217 627 616 1230 551 548 1455
Cuts at: 6187 618
Size: 1842
Fragments arranged by size:
1842 1455 1230 627 616 551 548 325
217
BanII G_rGCy'C
Cuts at: 1391 2032 2088 4302 4730 1391
Size: 641 56 2214 428 4072
Fragments arranged by size:
4072 2214 641 428 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2469 4134 4598 4713 928
Size: 971 570 1665 464 115 3626
Fragments arranged by size:
3626 1665 971 570 464 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4318 5303 6513 4318
Size: 985 1210 5216
Fragments arranged by size:
5216 1210 985
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6022 136
Size: 107 122 71 631 605 4350 1525
Fragments arranged by size:
4350 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4218 7406 4218
Size: 3188 4223
BlpI GC'TnA_GC
Cuts at: 4828 4828
Size: 7411
Bme1580I G_kGCm'C
Cuts at: 1790 5472 6628 1790
Size: 3682 1156 2573
Fragments arranged by size:
3682 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2529 5142 6100
Size: 416 53 83 399 24 1112 2613 958
Cuts at: 6100 442
Size: 1753
Fragments arranged by size:
2613 1753 1112 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2751 4286 6071 1239
Size: 1512 1535 1785 2579
Fragments arranged by size:
2579 1785 1535 1512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 5536 6401 6699 2869
Size: 2667 865 298 3581
Fragments arranged by size:
3581 2667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2675 4189 6074 882
Size: 617 1176 1514 1885 2219
Fragments arranged by size:
2219 1885 1514 1176 617
BsaAI yAC'GTr
Cuts at: 493 1204 2576 3145 5202 493
Size: 711 1372 569 2057 2702
Fragments arranged by size:
2702 2057 1372 711 569
BsaBI GATnn'nnATC
Cuts at: 2805 7405 2805
Size: 4600 2811
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5218 5600 6235 275
Size: 53 83 186 936 3685 382 635 1451
Fragments arranged by size:
3685 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3459 3636 5844 6242 6516 6663 7074 3459
Size: 177 2208 398 274 147 411 3796
Fragments arranged by size:
3796 2208 411 398 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3710 3740 4256 4286 1178
Size: 30 165 30 2307 30 516 30 4303
Fragments arranged by size:
4303 2307 516 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7411
BseYI C'CCAG_C
Cuts at: 2031 4485 6614 7110 2031
Size: 2454 2129 496 2332
Fragments arranged by size:
2454 2332 2129 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3123 1163
Size: 573 1387 5451
Fragments arranged by size:
5451 1387 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2025 4861 5622 6650 1540
Size: 275 186 24 2836 761 1028 2301
Fragments arranged by size:
2836 2301 1028 761 275 186 24
BsiHKAI G_wGCw'C
Cuts at: 2855 4302 5472 5557 6628 2855
Size: 1447 1170 85 1071 3638
Fragments arranged by size:
3638 1447 1170 1071 85
BsmI GAATG_Cn'
Cuts at: 2923 4293 4332 4529 4922 5015 2923
Size: 1370 39 197 393 93 5319
Fragments arranged by size:
5319 1370 393 197 93 39
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2088 2855 4302 4730 5472 5557
Size: 399 242 56 767 1447 428 742 85
Cuts at: 5557 6628 1391
Size: 1071 2174
Fragments arranged by size:
2174 1447 1071 767 742 428 399 242
85 56
BspEI T'CCGG_A
Cuts at: 7074 7074
Size: 7411
BspHI T'CATG_A
Cuts at: 3240 5300 7030 3240
Size: 2060 1730 3621
Fragments arranged by size:
3621 2060 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4766 844
Size: 3922 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 5298 1394
Size: 429 194 3281 3507
Fragments arranged by size:
3507 3281 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 5906 6080 65
Size: 1183 4658 174 1396
Fragments arranged by size:
4658 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3459 6055 6242 1700
Size: 110 1649 2596 187 2869
Fragments arranged by size:
2869 2596 1649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7411
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 5471 6483 5471
Size: 1012 6399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7411
BstBI TT'CG_AA
Cuts at: 6215 6215
Size: 7411
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7411
BstXI CCAn_nnnn'nTGG
Cuts at: 4283 4633 4283
Size: 350 7061
BstYI r'GATC_y
Cuts at: 1972 4218 4464 4741 5108 5493 5510 6962 7048
Size: 2246 246 277 367 385 17 1452 86
Cuts at: 7048 7060 7406 1972
Size: 12 346 1977
Fragments arranged by size:
2246 1977 1452 385 367 346 277 246
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4613 4613
Size: 7411
BtgI C'CryG_G
Cuts at: 513 1065 2004 2901 3845 513
Size: 552 939 897 944 4079
Fragments arranged by size:
4079 944 939 897 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4450 487
Size: 683 56 3224 3448
Fragments arranged by size:
3448 3224 683 56
BtsI GCAGTG_nn'
Cuts at: 4376 4927 5721 5741 4376
Size: 551 794 20 6046
Fragments arranged by size:
6046 794 551 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7376
DraI TTT'AAA
Cuts at: 4715 4715
Size: 7411
DraIII CAC_nnn'GTG
Cuts at: 4366 4366
Size: 7411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6418 809
Size: 507 5102 1802
Fragments arranged by size:
5102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2758 4858 5747
Size: 54 1425 50 275 18 928 2100 889
Cuts at: 5747 7376 8
Size: 1629 43
Fragments arranged by size:
2100 1629 1425 928 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3602 4290 4601 5341 1353
Size: 2249 688 311 740 3423
Fragments arranged by size:
3423 2249 740 688 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5998 6372 6518 387
Size: 1064 138 4409 374 146 1280
Fragments arranged by size:
4409 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4300 4300
Size: 7411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2751 3323 4187 4286 4392 5474
Size: 393 544 575 572 864 99 106 1082
Cuts at: 5474 6071 6858 7126 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1082 864 787 597 575 572 544
393 268 106 99
EcoNI CCTnn'n_nnAGG
Cuts at: 4142 4142
Size: 7411
EcoO109I rG'GnC_Cy
Cuts at: 2072 4267 2072
Size: 2195 5216
EcoRI G'AATT_C
Cuts at: 3507 4720 5098 3507
Size: 1213 378 5820
Fragments arranged by size:
5820 1213 378
EcoRV GAT'ATC
Cuts at: 2170 2818 2170
Size: 648 6763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2237
Size: 7411
FspI TGC'GCA
Cuts at: 5917 5917
Size: 7411
HaeII r_GCGC'y
Cuts at: 1678 2290 3594 6558 1678
Size: 612 1304 2964 2531
Fragments arranged by size:
2964 2531 1304 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 3151 3183 4464
Size: 32 135 32 484 32 1063 32 1281
Cuts at: 4464 4496 4576 4608 6052 6084 6126 6158 1373
Size: 32 80 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 1281 1063 484 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2713 3400 4755 5001 669
Size: 988 281 775 687 1355 246 3079
Fragments arranged by size:
3079 1355 988 775 687 281 246
HindIII A'AGCT_T
Cuts at: 748 2586 3390 748
Size: 1838 804 4769
Fragments arranged by size:
4769 1838 804
HpaI GTT'AAC
Cuts at: 5001 5001
Size: 7411
KpnI G_GTAC'C
Cuts at: 2407 3637 4736 2407
Size: 1230 1099 5082
Fragments arranged by size:
5082 1230 1099
MfeI C'AATT_G
Cuts at: 5010 5010
Size: 7411
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2005 2719 3019 6525 6709 1305
Size: 700 714 300 3506 184 2007
Fragments arranged by size:
3506 2007 714 700 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2760 10
Size: 54 1425 343 928 4661
Fragments arranged by size:
4661 1425 928 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4506 5369 5728 5887 518
Size: 648 3340 863 359 159 2042
Fragments arranged by size:
3340 2042 863 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2336 2662 4827 5504 6652 6897 1606
Size: 730 326 2165 677 1148 245 2120
Fragments arranged by size:
2165 2120 1148 730 677 326 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2901 3845 513
Size: 552 939 897 944 4079
Fragments arranged by size:
4079 944 939 897 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 2162 3295 4769 6314 1132
Size: 97 933 1133 1474 1545 2229
Fragments arranged by size:
2229 1545 1474 1133 933 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7411
PciI A'CATG_T
Cuts at: 6310 6310
Size: 7411
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 4366 1108
Size: 1734 1524 4153
Fragments arranged by size:
4153 1734 1524
PfoI T'CCnGG_A
Cuts at: 2079 2079
Size: 7411
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PmlI CAC'GTG
Cuts at: 2576 3145 2576
Size: 569 6842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5480 5512 6993 7025 4256
Size: 32 1192 32 1481 32 4642
Fragments arranged by size:
4642 1481 1192 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 4267 2072
Size: 2195 5216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7411
PsiI TTA'TAA
Cuts at: 3177 3438 4981 3177
Size: 261 1543 5607
Fragments arranged by size:
5607 1543 261
PstI C_TGCA'G
Cuts at: 830 4763 830
Size: 3933 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2336 2336
Size: 7411
SacI G_AGCT'C
Cuts at: 4302 4302
Size: 7411
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SapI GCTCTTCn'nnn_
Cuts at: 4290 4290
Size: 7411
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 3836 5659 7371 3836
Size: 1823 1712 3876
Fragments arranged by size:
3876 1823 1712
SfcI C'TryA_G
Cuts at: 826 1045 2907 3027 4759 5894 6575 6766 826
Size: 219 1862 120 1732 1135 681 191 1471
Fragments arranged by size:
1862 1732 1471 1135 681 219 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2848 5515 6416 6678 820
Size: 197 926 905 2667 901 262 1553
Fragments arranged by size:
2667 1553 926 905 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 4601 152
Size: 4449 2962
SphI G_CATG'C
Cuts at: 1132 3295 4769 1132
Size: 2163 1474 3774
Fragments arranged by size:
3774 2163 1474
SspI AAT'ATT
Cuts at: 5 52 3370 5335 5
Size: 47 3318 1965 2081
Fragments arranged by size:
3318 2081 1965 47
StuI AGG'CCT
Cuts at: 1517 4618 1517
Size: 3101 4310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2786 2901 3845 4212 4850 513
Size: 552 939 782 115 944 367 638 3074
Fragments arranged by size:
3074 944 939 782 638 552 367 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3519 4639 5438 5455 5608 5793 6238
Size: 1255 1939 1120 799 17 153 185 445
Cuts at: 6238 325
Size: 1498
Fragments arranged by size:
1939 1498 1255 1120 799 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3834 5657 7369
Size: 274 80 33 51 1443 1857 1823 1712
Cuts at: 7369 96
Size: 138
Fragments arranged by size:
1857 1823 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7371 7403 7371
Size: 32 7379
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7411
XbaI T'CTAG_A
Cuts at: 4747 4747
Size: 7411
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7411
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 3972 5540 3972
Size: 1568 5843
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5219 276
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI