Restriction map for pFN21AB3567
 (Linear) (Six-base) MAPSORT of: KIBB3567.seq  Check: 4197  from: 1  to: 2142



KIBB3567.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     831    2142 
   Size:        831    1311
 
AccI GT'mk_AC

Cuts at:      0    1334    2009    2142 
   Size:       1334     675     133

  Fragments arranged by size: 

                1334     675     133
 
Acc65I G'GTAC_C

Cuts at:      0     189    2142 
   Size:        189    1953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1635    1737    2142 
   Size:       1635     102     405

  Fragments arranged by size: 

                1635     405     102
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1239    2142 
   Size:       1239     903
 
AleI CACnn'nnGTG

Cuts at:      0      22      37    2142 
   Size:         22      15    2105

  Fragments arranged by size: 

                2105      22      15
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1273    2142 
   Size:       1273     869
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     982    1014    2142 
   Size:        982      32    1128

  Fragments arranged by size: 

                1128     982      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     201    2142 
   Size:        201    1941
 
ApaLI G'TGCA_C

Cuts at:      0     532    2142 
   Size:        532    1610
 
AvaI C'yCGr_G

Cuts at:      0    1409    1471    2142 
   Size:       1409      62     671

  Fragments arranged by size: 

                1409     671      62
 
BanI G'GyrC_C

Cuts at:      0     189    1117    1488    2142 
   Size:        189     928     371     654

  Fragments arranged by size: 

                 928     654     371     189
 
BanII G_rGCy'C

Cuts at:      0     736    1508    1973    1997    2142 
   Size:        736     772     465      24     145

  Fragments arranged by size: 

                 772     736     465     145      24
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1301    2142 
   Size:       1301     841
 
BglII A'GATC_T

Cuts at:      0    1046    2082    2142 
   Size:       1046    1036      60

  Fragments arranged by size: 

                1046    1036      60
 
Bme1580I G_kGCm'C

Cuts at:      0     536    1493    2142 
   Size:        536     957     649

  Fragments arranged by size: 

                 957     649     536
 
BmgBI CAC'GTC

Cuts at:      0     483    2142 
   Size:        483    1659
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      44     279     343     686    2142 
   Size:         44     235      64     343    1456

  Fragments arranged by size: 

                1456     343     235      64      44
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     750    2142 
   Size:        750    1392
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     745     747     939    1063    2142 
   Size:        579     166       2     192     124    1079

  Fragments arranged by size: 

                1079     579     192     166     124       2
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     700    1057    2142 
   Size:        592     108     357    1085

  Fragments arranged by size: 

                1085     592     357     108
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2041    2142 
   Size:       2041     101
 
BsaHI Gr'CG_yC

Cuts at:      0    1067    2142 
   Size:       1067    1075
 
BsaWI w'CCGG_w

Cuts at:      0     875    1347    1850    1871    2142 
   Size:        875     472     503      21     271

  Fragments arranged by size: 

                 875     503     472     271      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     921    1636    2142 
   Size:         22     899     715     506

  Fragments arranged by size: 

                 899     715     506      22
 
BseYI C'CCAG_C

Cuts at:      0      75     741    1161    1555    1812    2142 
   Size:         75     666     420     394     257     330

  Fragments arranged by size: 

                 666     420     394     330     257      75
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15      73     376    2142 
   Size:         15      58     303    1766

  Fragments arranged by size: 

                1766     303      58      15
 
BsiEI CG_ry'CG

Cuts at:      0    1305    1370    2009    2142 
   Size:       1305      65     639     133

  Fragments arranged by size: 

                1305     639     133      65
 
BsiHKAI G_wGCw'C

Cuts at:      0     333     536    1138    1262    1613    1973    2142 
   Size:        333     203     602     124     351     360     169

  Fragments arranged by size: 

                 602     360     351     333     203     169     124
 
BsmI GAATG_Cn'

Cuts at:      0     102    2142 
   Size:        102    2040
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1201    1412    2142 
   Size:       1201     211     730

  Fragments arranged by size: 

                1201     730     211
 
Bsp1286I G_dGCh'C

Cuts at:      0     333     536     736    1138    1262    1493    1508    1613 
   Size:        333     203     200     402     124     231      15     105

Cuts at:   1613    1973    1997    2142 
   Size:        360      24     145

  Fragments arranged by size: 

                 402     360     333     231     203     200     145     124
                 105      24      15
 
BspEI T'CCGG_A

Cuts at:      0     875    1347    1850    1871    2142 
   Size:        875     472     503      21     271

  Fragments arranged by size: 

                 875     503     472     271      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     506     831    1445    1745    2142 
   Size:        506     325     614     300     397

  Fragments arranged by size: 

                 614     506     397     325     300
 
BsrBI CCG'CTC

Cuts at:      0     729    2142 
   Size:        729    1413
 
BsrDI GCAATG_nn'

Cuts at:      0     359    1809    2142 
   Size:        359    1450     333

  Fragments arranged by size: 

                1450     359     333
 
BsrFI r'CCGG_y

Cuts at:      0      21     436    1365    2142 
   Size:         21     415     929     777

  Fragments arranged by size: 

                 929     777     415      21
 
BssHII G'CGCG_C

Cuts at:      0    1414    2142 
   Size:       1414     728
 
BstEII G'GTnAC_C

Cuts at:      0     660    2142 
   Size:        660    1482
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      75    2142 
   Size:         75    2067
 
BstYI r'GATC_y

Cuts at:      0    1046    1193    1580    2082    2142 
   Size:       1046     147     387     502      60

  Fragments arranged by size: 

                1046     502     387     147      60
 
BtgI C'CryG_G

Cuts at:      0     263    1139    2142 
   Size:        263     876    1003

  Fragments arranged by size: 

                1003     876     263
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     156    2142 
   Size:        156    1986
 
DraIII CAC_nnn'GTG

Cuts at:      0     399    2142 
   Size:        399    1743
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2005    2142 
   Size:       2005     137
 
EaeI y'GGCC_r

Cuts at:      0     356     411    1302    1367    1397    1925    2142 
   Size:        356      55     891      65      30     528     217

  Fragments arranged by size: 

                 891     528     356     217      65      55      30
 
EagI C'GGCC_G

Cuts at:      0    1302    1367    2142 
   Size:       1302      65     775

  Fragments arranged by size: 

                1302     775      65
 
EarI CTCTTCn'nnn_

Cuts at:      0     169    1543    1613    1654    2142 
   Size:        169    1374      70      41     488

  Fragments arranged by size: 

                1374     488     169      70      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1450    2142 
   Size:       1450     692
 
EcoICRI GAG'CTC

Cuts at:      0    1971    2142 
   Size:       1971     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     745     747     939    1063    1635    1737    2142 
   Size:        579     166       2     192     124     572     102     405

  Fragments arranged by size: 

                 579     572     405     192     166     124     102       2
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115    1441    2142 
   Size:        115    1326     701

  Fragments arranged by size: 

                1326     701     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     158     213     220     609    1341    1644    2142 
   Size:        158      55       7     389     732     303     498

  Fragments arranged by size: 

                 732     498     389     303     158      55       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1404    1665    2142 
   Size:       1404     261     477

  Fragments arranged by size: 

                1404     477     261
 
FspI TGC'GCA

Cuts at:      0     682    2142 
   Size:        682    1460
 
HaeII r_GCGC'y

Cuts at:      0     566     724    1296    2142 
   Size:        566     158     572     846

  Fragments arranged by size: 

                 846     572     566     158
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     598     630     916     948    1225    1257    2142 
   Size:        598      32     286      32     277      32     885

  Fragments arranged by size: 

                 885     598     286     277      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2010    2142 
   Size:       2010     132
 
KpnI G_GTAC'C

Cuts at:      0     193    2142 
   Size:        193    1949
 
MscI TGG'CCA

Cuts at:      0    1399    1927    2142 
   Size:       1399     528     215

  Fragments arranged by size: 

                1399     528     215
 
MslI CAynn'nnrTG

Cuts at:      0      22      37      95    2142 
   Size:         22      15      58    2047

  Fragments arranged by size: 

                2047      58      22      15
 
MspA1I CmG'CkG

Cuts at:      0      63     132     456    1141    1659    2127    2142 
   Size:         63      69     324     685     518     468      15

  Fragments arranged by size: 

                 685     518     468     324      69      63      15
 
NaeI GCC'GGC

Cuts at:      0     438    1367    2142 
   Size:        438     929     775

  Fragments arranged by size: 

                 929     775     438
 
NdeI CA'TA_TG

Cuts at:      0     957    2142 
   Size:        957    1185
 
NgoMIV G'CCGG_C

Cuts at:      0     436    1365    2142 
   Size:        436     929     777

  Fragments arranged by size: 

                 929     777     436
 
PasI CC'CwG_GG

Cuts at:      0     890    2142 
   Size:        890    1252
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     201    1172    2142 
   Size:        201     971     970

  Fragments arranged by size: 

                 971     970     201
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2038    2070    2142 
   Size:       2038      32      72

  Fragments arranged by size: 

                2038      72      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     158     213     220    1341    2142 
   Size:        158      55       7    1121     801

  Fragments arranged by size: 

                1121     801     158      55       7
 
PshAI GACnn'nnGTC

Cuts at:      0     218    2142 
   Size:        218    1924
 
PstI C_TGCA'G

Cuts at:      0     152    1252    2142 
   Size:        152    1100     890

  Fragments arranged by size: 

                1100     890     152
 
PvuII CAG'CTG

Cuts at:      0     132     456    1659    2127    2142 
   Size:        132     324    1203     468      15

  Fragments arranged by size: 

                1203     468     324     132      15
 
SacI G_AGCT'C

Cuts at:      0    1973    2142 
   Size:       1973     169
 
SacII CC_GC'GG

Cuts at:      0    1142    2142 
   Size:       1142    1000
 
SalI G'TCGA_C

Cuts at:      0    2008    2142 
   Size:       2008     134
 
SanDI GG'GwC_CC

Cuts at:      0     158     220    2142 
   Size:        158      62    1922

  Fragments arranged by size: 

                1922     158      62
 
SfcI C'TryA_G

Cuts at:      0     148    1248    2142 
   Size:        148    1100     894

  Fragments arranged by size: 

                1100     894     148
 
SgrAI Cr'CCGG_yG

Cuts at:      0      21    2142 
   Size:         21    2121
 
SmaI CCC'GGG

Cuts at:      0    1473    2142 
   Size:       1473     669
 
SmlI C'TyrA_G

Cuts at:      0     571     715    1072    2142 
   Size:        571     144     357    1070

  Fragments arranged by size: 

                1070     571     357     144
 
StuI AGG'CCT

Cuts at:      0     178     580    2142 
   Size:        178     402    1562

  Fragments arranged by size: 

                1562     402     178
 
StyI C'CwwG_G

Cuts at:      0     587    1745    2142 
   Size:        587    1158     397

  Fragments arranged by size: 

                1158     587     397
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     465    1164    2142 
   Size:        465     699     978

  Fragments arranged by size: 

                 978     699     465
 
TatI w'GTAC_w

Cuts at:      0      26    2142 
   Size:         26    2116
 
Tth111I GACn'n_nGTC

Cuts at:      0     484    2142 
   Size:        484    1658
 
XbaI T'CTAG_A

Cuts at:      0     771    2142 
   Size:        771    1371
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108    1169    2142 
   Size:        108    1061     973

  Fragments arranged by size: 

                1061     973     108
 
XmaI C'CCGG_G

Cuts at:      0    1471    2142 
   Size:       1471     671
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI     AlfI     AloI
    AlwNI    ApaLI     AvaI     BanI    BanII     BglI    BglII Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstEII    BstXI
    BstYI     BtgI    BtgZI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII     KpnI     MscI     MslI   MspA1I     NaeI     NdeI
   NgoMIV     PasI    PflMI     PpiI    PpuMI    PshAI     PstI    PvuII
     SacI    SacII     SalI    SanDI     SfcI    SgrAI     SmaI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     ApaI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI   Bpu10I
    BsaAI    BsaBI    BsaXI    BsiWI    BspHI    BsrGI    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI    EcoRI
    EcoRV     FseI    FspAI  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     NarI     NcoI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XhoI     XmnI     ZraI
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